Optika B-1000 Series User manual
Ver. 1.2 2022
Model
B-1000LD4
B-1000 Series
INSTRUCTION MANUAL
Page 2
Table of contents
1. Warning 3
2. Safety Information 3
3. Package content 4
3.1 Manual version 4
3.2 Motorized version 5
4. Unpacking 6
5. Intended use 6
6. Symbols and conventions 6
7. Instrument description 7
7.1 Manual version 7
7.2 Motorized version 9
8. Assembling 11
8.1 Assembling the microscope 11
8.1.1 Manual version 11
8.1.2 Installingadditionaluorescencelter 13
8.1.3 Replacingauorescencelter 14
8.1.4 Motorized version 15
9. Brightfield (transmitted light) observation procedures 16
10. Use of the microscope in Brightfield (transmitted light) 17
10.1 General switch on 17
10.2 Control keyboard 17
10.3 Brightness adjustment 17
10.4 Adjust the observation head 18
10.5 Adjust the interpupillary distance 18
10.6 Diopter adjustment 18
10.7 Use of eyeshields 18
10.8 Light path selection 19
10.9 Coarse focus tension adjustment 19
10.10 Focus stop lever 20
10.11 Stage 20
10.12 Centering the condenser 21
10.13Eectofelddiaphragm 21
10.14 Aperture diaphragm 21
10.15 Use of oil immersion objective 22
10.16 Only for motorized version 23
10.16.1 Nosepiece rotation 23
10.16.2 Focusing 23
10.16.3 Stage 23
11. Fluorescence (reflected light) observation procedures 24
12. Use of the microscope in Fluorescence (reflected light) 25
12.1 LED switch on 25
12.2 Useofuorescence 25
12.3 Use of light excluding plate 26
12.4 Use of UV shield 26
13. Use of universal condenser for Brightfield / Darkfield / Phase contrast 27
13.1 Brighteldobservation(BF) 27
13.2 Darkeldobservation(DF) 27
13.3 Phase Contrast observation (PH) 28
13.4 Useofgreenlter 29
14. DIC observation 30
14.1 Koehler DIC transmitted light 30
14.2 Nomarski DIC transmitted light 31
15. Simultaneous observation in Phase Contrast + Fluorescence 33
16. Microphotography 34
16.1 Use of “C”mount camera 34
16.2UseofReexcamera 34
17. Maintenance 35
18. Troubleshooting 36
Equipment disposal 38
Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.
Page 4
3. Package content
3.1 Manual version
①
②
③
④
⑤
⑥
⑦
⑧
① Frame
② Objectives
③ Stage
④ Condenser(dependingontheconguration)
⑤ Observationhead
⑥ Eyepieces
⑦ LEDuorescenceattachment
⑧ Lightexcludingplate
⑨ Dustcover
⑩ Allenwrench
⑪ Immersionoil(if100xisincludedinthecongu-
ration)
⑫Powersupply
• 6Vfortransmittedlight
• 12Vforuorescence
⑬ UVshield
⑪
⑩
⑨
⑫
⑬
Page 5
3.2 Motorized version
①
②
③
④
⑤
⑥
⑦
⑧
① Frame
② Objectives
③ Stage
④ Condenser(dependingontheconguration)
⑤ Observationhead
⑥ Eyepieces
⑦ LEDuorescenceattachment
⑧ Lightexcludingplate
⑨ Dustcover
⑩ Allenwrench
⑪ Immersionoil(if100xisincludedinthecongu-
ration)
⑫Powersupply
• 6Vfortransmittedlight
• 12Vforuorescence
⑬UVshield
⑭ Motorizedpartspowersupply
⑮ Serialcable
⑯ PS/2mouse
⑪⑩
⑨
⑫
⑬
⑭⑮
⑯
Page 6
4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Page 7
COARSE
FOCUSING
KNOB
NOSEPIECE
DIOPTER
ADJUSTMENT
RING
PHOTO/TV
PORT
MAIN SWITCH
EYEPIECE
FILTER
SELECTOR
LEVER
TRANSMITTED
LIGHT BRIGHTNESS
ADJUSTMENT KNOB
FOCUS
LOCK
LEVER
FINE
FOCUSING
KNOB
OBJECTIVE
STAGE
7. Instrument description
CONTROL
KEYBOARD
7.1 Manual version
UV SHIELD
CONDENSER
Page 8
FIELD
DIAPHRAGM
APERTURE
DIAPHRAGM
Opposite side
FLUORESCENCE
MAIN SWITCH /
BRIGHTNESS
ADJUSTMENT
KNOB
LIGHT PATH
SELECTOR
LEVER
TENSION
ADJUSTMENT
RING
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
X-Y STAGE
MOVEMENT
KNOBS
DIC SLIDER
SLOT
Page 9
7.2 Motorized version
Only the parts related to the motorized version are highlighted.
NOSEPIECE
FOCUS
MOTOR
X-AXIS MOVEMENT
KNOB
(MANUAL MOVEMENT)
Page 10
Opposite side
Y-AXIS MOVEMENT KNOB
(MANUAL MOVEMENT)
NOSEPIECE
ROTATION
BUTTONS
STAGE
CONNECTION
CABLE
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