Optika B-1000 Series User manual

Ver. 1.2 2022

Model

B-1000LD4

B-1000 Series

INSTRUCTION MANUAL

Page 2

Table of contents

1. Warning 3

2. Safety Information 3

3. Package content 4

3.1 Manual version 4

3.2 Motorized version 5

4. Unpacking 6

5. Intended use 6

6. Symbols and conventions 6

7. Instrument description 7

7.1 Manual version 7

7.2 Motorized version 9

8. Assembling 11

8.1 Assembling the microscope 11

8.1.1 Manual version 11

8.1.2 Installingadditionaluorescencelter 13

8.1.3 Replacingauorescencelter 14

8.1.4 Motorized version 15

9. Brightfield (transmitted light) observation procedures 16

10. Use of the microscope in Brightfield (transmitted light) 17

10.1 General switch on 17

10.2 Control keyboard 17

10.3 Brightness adjustment 17

10.4 Adjust the observation head 18

10.5 Adjust the interpupillary distance 18

10.6 Diopter adjustment 18

10.7 Use of eyeshields 18

10.8 Light path selection 19

10.9 Coarse focus tension adjustment 19

10.10 Focus stop lever 20

10.11 Stage 20

10.12 Centering the condenser 21

10.13E󰀨ectofelddiaphragm 21

10.14 Aperture diaphragm 21

10.15 Use of oil immersion objective 22

10.16 Only for motorized version 23

10.16.1 Nosepiece rotation 23

10.16.2 Focusing 23

10.16.3 Stage 23

11. Fluorescence (reflected light) observation procedures 24

12. Use of the microscope in Fluorescence (reflected light) 25

12.1 LED switch on 25

12.2 Useofuorescence 25

12.3 Use of light excluding plate 26

12.4 Use of UV shield 26

13. Use of universal condenser for Brightfield / Darkfield / Phase contrast 27

13.1 Brighteldobservation(BF) 27

13.2 Darkeldobservation(DF) 27

13.3 Phase Contrast observation (PH) 28

13.4 Useofgreenlter 29

14. DIC observation 30

14.1 Koehler DIC transmitted light 30

14.2 Nomarski DIC transmitted light 31

15. Simultaneous observation in Phase Contrast + Fluorescence 33

16. Microphotography 34

16.1 Use of “C”mount camera 34

16.2UseofReexcamera 34

17. Maintenance 35

18. Troubleshooting 36

Equipment disposal 38

Page 3

1. Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It

is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains

important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.

We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.

2. Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage

of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The

equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please

follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 4

3. Package content

3.1 Manual version

①

②

③

④

⑤

⑥

⑦

⑧

① Frame

② Objectives

③ Stage

④ Condenser(dependingontheconguration)

⑤ Observationhead

⑥ Eyepieces

⑦ LEDuorescenceattachment

⑧ Lightexcludingplate

⑨ Dustcover

⑩ Allenwrench

⑪ Immersionoil(if100xisincludedinthecongu-

ration)

⑫Powersupply

• 6Vfortransmittedlight

• 12Vforuorescence

⑬ UVshield

⑪

⑩

⑨

⑫

⑬

Page 5

3.2 Motorized version

①

②

③

④

⑤

⑥

⑦

⑧

① Frame

② Objectives

③ Stage

④ Condenser(dependingontheconguration)

⑤ Observationhead

⑥ Eyepieces

⑦ LEDuorescenceattachment

⑧ Lightexcludingplate

⑨ Dustcover

⑩ Allenwrench

⑪ Immersionoil(if100xisincludedinthecongu-

ration)

⑫Powersupply

• 6Vfortransmittedlight

• 12Vforuorescence

⑬UVshield

⑭ Motorizedpartspowersupply

⑮ Serialcable

⑯ PS/2mouse

⑪⑩

⑨

⑫

⑬

⑭⑮

⑯

Page 6

4. Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the

top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-

aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it

on a stable desk.

Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other

residuals may deteriorate the nal image quality and corrode the optics surface in a short time.

5. Intended use

Standard models

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

IVD Models

Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.

6. Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

Page 7

COARSE

FOCUSING

KNOB

NOSEPIECE

DIOPTER

ADJUSTMENT

RING

PHOTO/TV

PORT

MAIN SWITCH

EYEPIECE

FILTER

SELECTOR

LEVER

TRANSMITTED

LIGHT BRIGHTNESS

ADJUSTMENT KNOB

FOCUS

LOCK

LEVER

FINE

FOCUSING

KNOB

OBJECTIVE

STAGE

7. Instrument description

CONTROL

KEYBOARD

7.1 Manual version

UV SHIELD

CONDENSER

Page 8

FIELD

DIAPHRAGM

APERTURE

DIAPHRAGM

Opposite side

FLUORESCENCE

MAIN SWITCH /

BRIGHTNESS

ADJUSTMENT

KNOB

LIGHT PATH

SELECTOR

LEVER

TENSION

ADJUSTMENT

RING

CONDENSER

HEIGHT

ADJUSTMENT

KNOB

X-Y STAGE

MOVEMENT

KNOBS

DIC SLIDER

SLOT

Page 9

7.2 Motorized version

Only the parts related to the motorized version are highlighted.

NOSEPIECE

FOCUS

MOTOR

X-AXIS MOVEMENT

KNOB

(MANUAL MOVEMENT)

Page 10

Opposite side

Y-AXIS MOVEMENT KNOB

(MANUAL MOVEMENT)

NOSEPIECE

ROTATION

BUTTONS

STAGE

CONNECTION

CABLE

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