Optika B-1000 Series User manual

Model

B-1000

B-1000BF

B-1000PH

B-1000TI-2

B-1000TI-3

B-1000TI-5

B-1000TI-10

B-1000 Series

INSTRUCTION MANUAL

Ver. 3.2 2020

Page 2

1. Warning 3

2. Symbols and conventions 3

3. Safety Information 3

4. Intended use 3

5. Instrument description 4

5.1 Manual version 4

5.2 Motorized version 6

5.3 B-1000TI-2 / 3 / 5 / 10 8

6. Unpacking 9

7. Assembling 9

7.1 Manual version 9

7.2 Motorized version 10

7.3 B-1000TI-2/3/5/10 11

7.4 Assembling the microscope 12

7.4.1 Manual version 12

7.4.2 Motorized version 14

7.4.3 B-1000TI-2 / 3 / 5 / 10 15

8. SummaryofBrighteldobservationprocedures 18

9. Use of the microscope 19

9.1 General switch on 19

9.2 Control keyboard 19

9.3 Brightness adjustment 19

9.4 Adjust the observation head 20

9.5 Adjust the interpupillary distance 20

9.6 Diopter adjustment 20

9.7 Use of eyeshields 20

9.8 Light path selection 21

9.9 Coarse focus tension adjustment 21

9.10 Focus stop lever 22

9.11 Stage 22

9.12 Centering the condenser 23

9.13 Effectofelddiaphragm 23

9.14 Aperture diaphragm 23

9.15 Use of oil immersion objective 24

9.16 Use of ALC system (optional) 24

9.17 Only for motorized version 25

9.17.1 Nosepiece rotation 25

9.17.2 Focusing 25

9.17.3 Stage 25

9.18 Use of the pointer (B-1000TI-2 / 3 / 5 / 10) 26

10. UseofuniversalcondenserforBrighteld/Darkeld/Phasecontrast 27

10.1 Brighteldobservation(BF) 27

10.2 Darkeldobservation(DF) 27

10.3 PhaseContrastobservation(PH) 28

10.4 Useofgreenlter 29

11. DIC observation 30

11.1 Koehler DIC transmitted light 30

11.2 Nomarski DIC transmitted light 31

12. Microphotography 33

12.1 Use of C-mount cameras 33

12.2 Useofreexcamera 33

13. Maintenance 34

14. Troubleshooting 35

Equipment disposal 37

Summary

Page 3

1. Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of

maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you

that this manual contains important information on safety and maintenance, and that it must therefore be made

accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses

that does not comply with this manual.

2. Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICALSHOCK

This symbol indicates a risk of electrical shock.

3. Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety

regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility

to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure

safe operation of the unit.

4. Intended use

Standard models

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

IVD Models

Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the

subject.

Page 4

EYEPIECE

PHOTO / TV

PORT

OBSERVATION

HEAD

ALC SYSTEM

DIOPTER

ADJUSTMENT

RING

DIC PRISM

SLOT

CONTROL

KEYBOARD

MAIN

SWITCH

FOCUS LOCK

LEVER INTENSITY

ADJUSTMENT

DIAL

FIELD

DIAPHRAGM

CONDENSER

STAGE

CONDENSER

HEIGHT

ADJUSTMENT

KNOB

NOSEPIECE

OBJECTIVES

5. Instrument description

5.1 Manual version

Page 5

FILTER

HOLDER

COARSE

FOCUS KNOB

FINE FOCUS

KNOB

TENSION

ADJUSTMENT

RING

APERTURE

DIAPHRAGM

LIGHT PATH

SELECTOR

LEVER

SLIDE

HOLDER

X-Y STAGE

MOVEMENT

KNOBS

CONDENSER

CENTERING

SCREWS

ALC SOCKET

Opposite side

Page 6

NOSEPIECE

FOCUS

MOTOR

X-AXIS MOVEMENT

KNOB

(MANUAL MOVEMENT)

STAGE

5.2 Motorized version

Only the parts related to the motorized version are highlighted.

Page 7

STAGE

CONNECTION

CABLE

NOSEPIECE

ROTATION

BUTTONS

Y-AXIS MOVEMENT

KNOB

(MANUAL MOVEMENT)

Opposite side

Page 8

POINTER BRIGHTNESS

ADJUSTMENT / POINTER

MOVEMENT

SIDE

OBSERVATION

HEADS

POINTER COLOR

SELECTOR

5.3 B-1000TI-2 / 3 / 5 / 10

Only the multi-head parts are highlighted.

Page 9

① Frame

② Objectives

③ Stage

④ Condenser

⑤ Observation head

⑥ Eyepieces

⑦ ALC system (M-1030) (Optional)

⑧ Power supply

⑨ Dust cover

⑩ Allen wrench

⑪ Immersion oil

③

④

⑤

⑥

⑦

⑧

①

②

⑪

⑩

⑨

6. Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container

and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)

fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope

from the container and put it on a stable desk.

Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or

other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.

7. Assembling

Once opened the box, the microscope parts are the following:

7.1 Manual version

Page 10

⑪⑩

⑨

① Frame

② Objectives

③ Stage

④ Condenser

⑤ Observation head

⑥ Eyepieces

⑦ ALC system (M-1030) (Optional)

⑧ Microscope power supply

⑨ Motorized parts power supply

⑩ Serial cable

⑪ PS/2 mouse

⑫ Dust cover

⑬ Allen wrench

⑭ Immersion oil

①②

③

④

⑤

⑥

⑦

⑧

⑫⑬⑭

7.2 Motorized version

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