Optika IM Series User manual

Version: 3

Issued: 03, 05, 2019

Model

IM-3LD

IM Series

INSTRUCTION MANUAL

Page 2

Table of Contents

Warning

Symbols and conventions

Safety Information

Intended use

List of accessories and spare parts

Overview

Unpacking

Assembling

Using the microscope

Maintenance

Troubleshooting

Equipment disposal

Page 3

Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-

nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this

manual contains important information on safety and maintenance, and that it must therefore be made acces-

sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does

not comply with this manual.

Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety

regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-

ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure

safe operation of the unit.

Intended use

Standard models

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

IVD Models

Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the sub-

ject.

Page 4

List of accessories and spare parts

CAT. NO. DESCRIPTION

M-780 Eyepiece EWF10x/22mm.

M-781 Eyepiece micrometer EWF10x/22mm.

M-005 26x76 mm micrometric slide. Range 1 mm, div. 0,01 mm.

M-782 Objective IOS LWD PLAN Achromatic 4x/0,10 (w.d. 22mm).

M-782.1 Objective IOS LWD PLAN Achromatic for phase contrast 4x/0.13 (w.d. 16.9mm).

M-783N Objective IOS LWD PLAN Achromatic for phase contrast 10x/0,25 (w.d. 7,94mm).

M-784N Objective IOS LWD PLAN Achromatic for phase contrast 20x/0,40 (w.d. 7,66mm).

M-785 Objective IOS LWD PLAN Achromatic for phase contrast 40x/0,60 (w.d. 3,71mm).

M-786 Objective IOS LWD PLAN Achromatic 60x/0,70 (w.d. 2,50mm).

M-801 Objective IOS LWD FLUOR PLAN Achromatic 10x/0,30 (w.d. 10mm)

M-802 Objective IOS LWD FLUOR PLAN Achromatic 20x/0,45 (w.d. 5,1mm)

M-803 Objective IOS LWD FLUOR PLAN Achromatic 40x/0,65 (w.d. 2,6mm)

M-804 Objective IOS LWD FLUOR PLAN Achromatic 60x/0,7

M-785.2N Slider with phase rings (4x/10x, 20x/40x, BF position)

M-788 Photo adapter for REFLEX camera with FULL FRAME sensor.

M-789 Focusable C-Mount adapter for 1/3” sensor.

M-789.1 Focusable C-Mount adapter for 1/2” sensor.

M-789.2 Focusable C-Mount adapter for 2/3” sensor.

M-699 Universal adapter for M-114, M-115, M-116, M-173 and eyepiece cameras.

DC-004 TNT dust cover, large - 700(l)x550(h) mm.

M-792 Mechanical stage for IM-3 series

M-793.1 Holder for Petri diameter 38mm (M-793.2 needed).

M-793.2 Holder for Terasaki and Petri diameter 65mm.

M-793.3 Holder for slide and Petri diameter 54mm.

M-793.4 Holder for 2+2 slides.

M-793.6 Holder for Utermöhl-Chamber (M-793.3 needed).

M-793.7 Load-bearing side extension for IM-3 series.

M-173 Photo adapter for APS-C and Full Frame Reflex cameras.

M-114 C-Mount adapter for 1/2” sensor.

M-115 C-Mount adapter for 1/3” sensor.

M-116 C-Mount adapter for 2/3” sensor.

VP-IM IQ/OQ/PQ Validation Protocols.

Page 5

Overview

TRINOCULAR

VIEWING

TUBE

(FIXED)

DIOPTRIC

ADJUSTMENT

RING

EYEPIECE

PHASE

CONTRAST

SLIDER

LED

HOUSING

FILTER HOLDER

COARSE

FOCUS KNOB

FINE FOCUS KNOB

TENSION

ADJUSTMENT COLLAR

OBJECTIVE

STAGE

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

GLASS STAGE

INSERT

ANTI-GLOW CAP

CONDENSATOR

Page 6

Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container

and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)

fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope

from the container and put it on a stable desk.

Assembling

Once you open the box, these are the microscope’s components:

① Microscope body

② Condenser

③ LED illuminator

④ Filter protection covers

⑤ Anti-glow cap

⑥ Filter holder

⑦ Glass insert for stage

⑧ Fluorescence lters

⑨ Objectives

⑩ Eyepieces

⑪ Power supply

①

②

⑪

⑩

③

④

⑤

⑥⑦

⑧

⑨

Page 7

③

Fig.2

Fig.3

Fig.4

Fig.5

Fig.1

1. Turning the coarse focusing knob ① till the

nosepiece reaches its lowest position.

► For a safe transport, the nosepiece is

placed in the lowest position and the

tension adjustment collar ②is adjust-

ed to the appropriate tension when the

microscope leaves the factory. (Fig.1)

2. Screw the lowest magnication objective

on to the turret from the right side, then

turn the turret clockwise. Mount the other

objectives in the same way, following the

sequence from low to high.

► Note: the objectives can also be in-

stalled through the stage opening.

(Fig.2)

► Clean the objectives regularly. In inverted

microscopes, the objectives are very sen-

sitive to dust.

► To prevent dust and contamination from

entering the microscope, cover all the un-

used holes with dust caps ③. (Fig.3)

► When operating, use the low magnication

objective (10X) to search and focus the

specimen, then switch to higher magnica-

tions.

► When switching between objectives, slowly

turn the nosepiece until it clicks. The click

means that the objective is in the right posi-

tion, in the center of the light path.

①

②

Installing the objectives

The stage extension can be installed on either

side of the stage to enlarge the working sur-

face. The mechanical stage must be installed

on the side opposite the extension.

For right-handed operators, the mechanical

stage is normally installed on the right side.

1. Installing the stage extension: Screw the

bolts on to the extension, then mount the

extension from below the stage. (Fig.4)

2. Installing the mechanical stage: As for the

extension, the mechanical stage is xed

with two bolts under the stage. (Fig.5)

Installing the stage extension and

the mechanical stage (OPTIONAL)

Page 8

► Remove the lter holder, then install the

color lters you need. (Fig.11, Fig.12)

Mount the color lter at as shown in ①,

verifying that they are not tilted.

► If the color lter is tilted or otherwise

out of place ②, it may fall.

The color lters can be stacked in the

holder. This allows to install as many lters

as needed, as long as the whole thickness

is less than 11 mm.

1. When using the glass stage, make sure

that the insert is horizontal.

2. Install the stage insert in the stage

opening. (Fig.6)

Installing the color lters

Installing the stage insert

Installing the eyepieces

Insert both eyepieces into the tubes of the

optical head. (Fig.7)

Fig.6

Fig.11

Fig.12

Fig.7

Fig.8 Fig.9

Fig.10

1. Insert the condenser illumination unit into

the bracket. (Fig.8)

2. Turn the condenser illumination unit

clockwise about 90°, with the “AS” mark

of lter holder facing forwards. Align the

screw of the condenser illumination unit

and the hole of the holder, then screw

the bolt in the hole with the supplied allen

wrench. (Fig.9)

3. Insert the connector plug into the

connector jack.

4. Push the LED housing gently into the

holes of the illumination unit. (Fig.10)

Installing the condenser illumination

unit and the LED housing

①

②

Page 9

Installing the uorescence

Fig.13

• Unscrew the protection cover on the left

side of the microscope, with the provided

allen wrench. (Fig.14)

• When using the epiuorescence, you can

mount the anti-glow cap on the bottom of

the condenser, in order to increase the

contrast of the image. (Fig.13)

• Slide the green lter inside the dovetail

guide, after screwing the lter lever into

the threaded hole of the lter. Mount

the protection cover back in its position.

(Fig.15)

• On top of the lter lever, screw the terminal

with the etched G. Repeat the same steps

for the right side, mounting the Blue

lterset. (Fig.16)

Fig.14

Fig.15

Fig.16

• Connect the external power supply to the

input jack on the rear. (Fig.17)

Fig.17

Page 10

Turning on the LED

Connect the power, turn on the main switch ①.

(Fig.18)

Position I: BF

Position II: Epiuorescence

Adjusting the brightness

Turn the brightness adjustment knob ②to

increase and decrease the brightness. (Fig.19)

Adjusting the tension

► The coarse focusing knob ①is pre

adjusted to a tight tension upon leav-

ing the factory.

If the nosepiece drops down by itself, or the

specimen defocuses while adjusting the ne

focus knob ③, the coarse focus knob is too

loose. Turning the tension adjustment collar ②

in clockwise direction tightens the coarse fo-

cus tension ①. Rotate in the opposite direction

to decrease the tension. (Fig.20)

Setting the specimen

► For the best image quality, use asks,

Petri dishes and slides with a 1.2 mm

thickness.

1. Place the proper insert for your specimen

(according to the table on the right) on the

stage, and x it with the stage clip.

2. Turning the X and Y knobs, move the

specimen to the required position. (Move-

ment Range: 120 (width) × 78 (length)

mm).

Moving the specimen

Move the specimen to the desired position by

freehand or by turning the knobs of the me-

chanical stage.

► When switching objectives, take care

not to touch the adaptor plates with

the objectives, as their weight may

damage the front lens.

M-793.1

Holder for Petri diameter 38mm (M-793.2 needed)

M-793.2

Holder for Terasaki and Petri diameter 65mm.

M-793.3

Holder for slide and Petri diameter 54mm.

M-793.4

Holder for 2+2 slides.

M-793.6

Holder for Utermöhl-Chamber (M-793.3 needed).

M-792

Mechanical stage for IM-3 series.

INITIAL SETUP

STAGE (OPTIONAL)

Using the microscope

Fig.18

Fig.19

②

①

②

③

Fig.20

M-793.7

Load-bearing side extension for IM-3 series.

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