Optika B-500BSp User manual

Version: 7

Issued: 16, 03, 2017

Model

B-500BSp

B-500TSp

B-500Bpl

B-500Tpl

B-500Bi

B-500Ti

B-500ERGO

B-500iERGO

B-500Bph

B-500Tph

B-500Biph

B-500Tiph

B-500Ti-2

B-500Ti-3

B-500Ti-5

B-500ASB

B-500Ti2F

500 Series

INSTRUCTION MANUAL

Page 2

Table of Contents

Warning

Symbols and conventions

Safety Information

Intended use

List of accessories and spare parts

Overview

Unpacking

Assembling

Using the microscope

Maintenance

Troubleshooting

Equipment disposal

Page 3

Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-

nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this

manual contains important information on safety and maintenance, and that it must therefore be made acces-

sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does

not comply with this manual.

Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety

regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-

ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure

safe operation of the unit.

Intended use

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4

List of accessories and spare parts

CAT. NO. DESCRIPTION

M-680 Ergo binocular head 30°-60°

M-625 Eyepiece EWF10x/22mm

M-601 Eyepiece WF15x/16mm

M-602 Eyepiece micrometer EWF10x/22mm

M-005 26x76 mm micrometric slide. Range 1 mm, div. 0,01 mm

M-613 Polarising set ( lters only)

M-614 Rotating table for polarising set

M-618 Dark eld condenser for dry objectives

M-616 Complete phase contrast set with plan obj. 10x, 20x, 40x, 100x

M-617 Complete phase contrast set with plan IOS obj. 10x, 20x, 40x, 100x

M-616.1 Single phase contrast set 40x, plan objective

M-617.1 Single phase contrast set 40x, plan IOS objective

M-619 Photo adapter for REFLEX camera with FULL FRAME sensor

M-699 Universal adapter for OPTIKAM & DIGI cameras (only models with eyepiece adapter, 23mm)

M-620 C-Mount adapter for 1/3” sensor.

M-620.1 C-Mount adapter for 1/2” sensor.

M-515 Halogen bulb 12V/20W, with dichroic mirror

M-034 Dust cover type 5

M-975 Blue filter, 45 mm diameter

M-977 Green filter, 45 mm diameter

M-979 Yellow filter, 45 mm diameter

M-989 Frosted glass fifi lter, 45 mm diameter

ST-036 Eyecups (pair) type 2

M-173 Photo adapter for APS-C and Full Frame Reflfl ex cameras

M-114 C-Mount adapter for 1/2” sensor.

M-116 C-Mount adapter for 2/3” sensor.

M-113.1 Ring adapter, 30mm (for monocular and binocular microscopes)

M-778 C-mount adapter for 1/3”,1/2” and 2/3” sensor

M-666.500 Heating stage, with digital temperature controller B-500 series

M-666.500ph Heating stage, with digital temperature controller B-500Ph

15008 OPTIKA immersion oil 10ml

Page 5

Overview

NOSEPIECE

DIOPTRIC

ADJUSTMENT

RING

EYEPIECE

CONDENSER

CENTERING

KNOBS

APERTURE

DIAPHRAGM

FIELD DIAPHRAGM

TRANSLATION KNOB

FINE FOCUSING

KNOB

COARSE

FOCUSING

KNOB

TENSION

ADJUSTEMENT

KNOB

OBJECTIVE

STAGE

Page 6

Put the microscope stand on a

solid table.

Insert the optical head abo-

ve the stand and tighten the

screw. (Fig.1)

Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container

and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)

fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope

from the container and put it on a stable desk.

Assembling

Once you open the box, these are the microscope’s components:

① Condenser

② Optical head

③ Main body

④ Eyepieces

⑤ Eyepiece cups

⑥ Stage

⑦ Objectives

①

②

③

④

⑤

⑥

⑦

Fig.1

Page 7

Mount the stage: lower the

support using the coarse

focus knob, then place the

stage and rmly tighten the

lock screw ①. (Fig.4)

Insert both eyepieces into

the tubes of the optical head.

(Fig.2)

Insert the condenser under

the stage: position until it is

well inserted into its holder

(under the condenser there is

a pin that must fully enter the

guide of the holder). (Fig. 3)

Screw each objective into

the thread of the nosepiece,

in order of magnication.

(Fig.5)

Fig.2

Fig.3

Fig.4

Fig.5

Page 8

Insert the power supply cable

on the connector at the rear.

(Fig.6)

Fig.6

Page 9

In order to activate the transmitted light illumi-

nator, insert the plug of the power supply cable

into the socket and turn on the main switch on

the back of the main body. Turn the brightness

adjustment knob to a brightness suitable for

observation. (Fig.7)

Loosen the lock-screw, turn

the observation head to

a comfortable position for

observation, and then lock the

lock-screw. (Fig.8)

LED settings

Adjust the observation

head

MAIN

SWITCH

Using the microscope

Fig.7

Fig.8

Hold the right and left parts of

the observation head using

both hands and adjust the

interpupillary distance by

turning the two parts until one

circle of light can be seen.

(Fig.9)

Adjust interpupillary

distance

Fig.9

Place the specimen on

the stage

Fix the specimen slide on the

mechanical stage using the

slide-clamp. Ensure that the

specimen is centred over the

stage opening. (Fig.10)

Fig.10

Page 10

Focus tension

adjustment

Turn the tension-adjust knob

to get a suitable tension for

the focus system. (Fig.11)

NOTE: if the tension is too lo-

ose, the stage could go lower

by itself or the focus easily

lost after ne adjustment. In

this case, rotate the knob in

order to increase the friction. Fig.11

Focus-stop knob

Loosen the focus-stop knob,

rotate the coarse focusing

knob to bring the slide into

focus with a 4X or 10X objecti-

ve. Then rotate the focus-stop

in order to block the height of

the stage. This simplies the

next focusing operations.

The focus-stop knob is also

useful to avoid accidental con-

tacts between objective and

specimen. (Fig.12) Fig.12

Diopter adjustment

Adjust the ne focusing knob

to get the image sharp and

clear while observing with

your right eye, then turn the

left diopter ring to a sharp

and clear image also with the

other eye. The highpoint eye-

pieces allow the user to wear

glasses. (Fig.13)

NOTE :For the optimal para-

focality of the image, it’s sug-

gested to wear your glasses

during the normal use of the

microscope. Fig.13

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