Optika IM Series User manual

Ver. 5.0 2019

Model

IM-3

IM-3F

IM-3FL4

IM-3LD

IM Series

INSTRUCTION MANUAL

Page 2

Table of Contents

1. Warning 3

2. Symbols and conventions 3

3. Safety Information 3

4. Intended use 3

5. Overview 4

5.1 IM-3 4

5.2 IM-3F / IM-3FL4 5

5.3 IM-3LD 6

6. Unpacking 7

7. Assembling 7

7.1 IM-3 7

7.2 IM-3F / IM-3FL4 8

7.3 IM-3FLD 9

7.4 Installation procedure 10

7.4.1 Installing the objectives 10

7.4.2 Installing stage extension or mechanical stage 10

7.4.3 Installing the stage insert 11

7.4.4 Installing the eyepieces 11

7.4.5 Installing condenser and LED housing 11

7.4.6Installingcolorlters 12

7.4.7 Connecting the power supply 12

7.4.8Installinguorescence 13

8. Brighteldobservationprocedures 16

9. Using the microscope (BF or PH) 17

9.1 Turning on the microscope 17

9.2 Adjusting the light intensity 17

9.3 Adjusting the coarse focus tension 17

9.4 Diopter adjustment 17

9.5 Adjusting interpupillary distance 18

9.6 Use of eyeshields 18

9.7 Selecting the light path 18

9.8 Mechanical stage and sample holders 19

9.9 Aperture diaphragm 20

9.10 Usingcolorlters 20

9.11 Phase contrast 20

9.11.1 Phase contrast slider 21

9.11.2 Centering the phase ring 21

10. Fluorescence observation procedures (IM-3F / IM-3FL4) 22

11. Fluorescence observation procedures (IM-3LD) 22

12. Using the microscope (FL) 23

12.1 Centering the mercury bulb 23

12.2 Centeringtheelddiaphragm 25

12.3 Using the microscope 25

12.3.1Switchinguorescenceltercubes 25

12.3.2 Filter holder / Shutter 27

12.3.3InsertingaNDlter 27

12.3.4Usingthelterslider 27

13. Simultaneous observation in Phase Contrast + HBO Fluorescence 28

14. Microphotography 29

14.1 Use of C-mount cameras 29

14.2 UseofReexcamera 29

15. Maintenance 30

16. Troubleshooting 31

Equipment disposal 33

Page 3

1. Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-

nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this

manual contains important information on safety and maintenance, and that it must therefore be made accessi-

ble to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does

not comply with this manual.

2. Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

3. Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the ope-

ration voltage of the equipment and that the lamp switch is in off position. Users should observe all safety regu-

lations of the region. The equipment has acquired the CE safety label. However, users have full responsibility to

use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure safe

operation of the unit.

4. Intended use

Standard models

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

IVD Models

Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the

subject.

Page 4

5. Overview

FILTER HOLDER

PHASE

CONTRAST SLIDER

PHOTO/TV

PORT

DIOPTRIC

ADJUSTMENT

RING

EYEPIECE

LED

HOUSING

CONDENSER

COARSE

FOCUS KNOB

FINE FOCUS KNOB

TENSION

ADJUSTMENT

COLLAR

OBJECTIVE

STAGE

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

GLASS STAGE

INSERT

5.1 IM-3

Page 5

5.2 IM-3F / IM-3FL4

PHOTO/TV

PORT

DIOPTRIC

ADJUSTMENT

RING

EYEPIECE

PHASE CONTRAST SLIDER

(OPTIONAL FOR IM-3FL4)

LED

HOUSING

CONDENSER

FILTER HOLDER

COARSE

FOCUS KNOB

FINE FOCUS KNOB

FLUORESCENCE

POWER SUPPLY

TENSION

ADJUSTMENT

COLLAR

OBJECTIVE

STAGE

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

GLASS STAGE INSERT

FLUORESCENCE

LAMP HOUSING

UV SHIELD

Page 6

5.3 IM-3LD

DIOPTRIC

ADJUSTMENT

RING

EYEPIECE

PHASE

CONTRAST

SLIDER

LED

HOUSING

FILTER HOLDER

COARSE

FOCUS KNOB

FINE FOCUS KNOB

TENSION

ADJUSTMENT COLLAR

OBJECTIVE

STAGE

MICROSCOPE

BODY

LIGHT PATH

SELECTOR

LEVER

GLASS INSERT

STAGE

ANTI-GLOW CAP

CONDENSER

PHOTO/TV

PORT

Page 7

6. Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container

and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)

fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope

from the container and put it on a stable desk.

7. Assembling

7.1 IM-3

Once you open the IM-3 box, these are the microscope’s components:

① Microscope body

② Condenser

③ Filter holder

④ LED illuminator

⑤ Phase contrast slider

⑥ Eyepieces

⑦ Centering telescope

⑧ Metal insert for stage

⑨ Glass insert for stage

⑩ Objectives

⑪ Power supply

⑫ Green lter IF550

⑪

⑫⑩

③④

⑤

⑥

⑦⑧ ⑨

①②

Page 8

① Microscope body

② Condenser

③ LED illuminator

④ Fluorescence power supply + cable

⑤ Fluorescence power cable

⑥ Filter holder

⑦ Metal insert for stage

⑧ Glass insert for stage

⑨ Objectives

⑩ Eyepieces

⑪ Fluorescence lters

⑫ Green lter IF550

⑬ UV shield

⑭ Fluorescence illuminator

⑮ Mercury lamp house

⑯ Microscope power supply

⑰ Phase contrast slider

Provided with IM-3F

Optional with IM-3FL4

①

②

⑪

⑫

⑬⑭

⑮

⑩

③

④

⑤⑥

⑦ ⑧

⑨

7.2 IM-3F / IM-3FL4

Once you open the IM-3F / IM-3FL4 box, these are the microscope’s components:

⑯

⑰

Page 9

7.3 IM-3FLD

Once you open the IM-3LD box, these are the microscope’s components:

① Microscope body

② Condenser

③ LED illuminator

④ Filter protection covers

⑤ Anti-glow cap

⑥ Phase contrast slider

⑦ Glass insert for stage

⑧ Fluorescence lters

⑨ Objectives

⑩ Eyepieces

⑪ Power supply

①

②

⑪

⑩

③

④

⑤

⑥⑦

⑧

⑨

Page 10

7.4 Installation procedure

7.4.1 Installing the objectives

1. Turn the coarse focusing knob ① until the nose-

piece reaches its lowest position.

• For a safe transport, the nosepiece is placed

in the lowest position and the tension adjust-

ment collar ②is adjusted to the appropriate

tension when the microscope leaves the fac-

tory. (Fig.1)

2. Screw the lowest magnication objective on the

nosepiece from the right side, then turn the no-

sepiece clockwise. Mount the other objectives in

the same way, following the sequence from low

to high.

• Note: the objectives can also be installed

through the stage opening. (Fig. 2)

• Clean the objectives regularly. In inverted micro-

scopes, the objectives are very sensitive to dust.

• To prevent dust and contamination from entering

the microscope, cover all the unused holes with

dust caps ③. (Fig. 3)

• When operating, use the low power objective

(10X) to search and focus the specimen, then

switch to higher magnications.

• When switching between objectives, slowly turn

the nosepiece until it clicks. The click means that

the objective is in the right position, in the center

of the light path.

Fig. 1

①

②

Fig. 2

Fig. 3

③

7.4.2 Installing stage extension or mechanical stage

• Stage extension and mechanical stage are

optional accessories for all the models.

Stage extension can be installed on either side of the

stage to enlarge the working surface.

Mechanical stage can only be installed on the right

side of the stage.

1. Installing the units: screw the bolts in the xing

holes of the attachments, then mount the unit

from below the stage. (Fig. 4)

Fig. 4

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