Optika B-350 User manual

OPTIKA MICROSCOPES - ITALY

www.optikamicroscopes.com - [email protected]

Ver. 5.0.0

OPERATION MANUAL

GUIDA UTENTE

MANUEL D’INSTRUCTIONS

B-350

MANUAL DE INSTRUCCIONES

BEDIENUNGSANLEITUNG

Page 2

INDEX

1.0 DESCRIPTION pag. 3

2.0 INTRODUCTION pag. 6

3.0 UNPACKING AND ASSEMBLY pag. 7

4.0 USING THE MICROSCOPE pag. 9

4.1 Observation head

4.2 Place the specimen on the stage

4.3 Illumination system settings

4.4 Interpupillary distance

4.5 Focus tension adjustment

4.6 Diopter adjustment

4.7 Condenser

4.8 Numerical aperture setting

4.9 Phase rings centering (models B-352Ph and B353Ph)

 4.10Additionallters

5.0 MAINTENANCE

pag. 12

5.1 Microscopy environment

5.2 Before and after using the microscope

5.3 Precautions for a safe use

5.4 Cleaning the optics

6.0 TECHNICAL SPECIFICATIONS pag 13

7.0 RECOVERY AND RECYCLING pag 14

Page 3

1.0 DESCRIPTION

BINOCULAR OBSERVATION

HEAD

HEAD LOCKING

SCREW (1)

REVOLVING NOSEPIECE

STAGE

TRANSLATION KNOBS

MAIN BODY

BRIGHTNESS

ADJUSTMENT

CONDENSER CENTERING

SCREWS (2)

LED

ILLUMINATOR

IRIS DIAPHRAGM

(3)

EYEPIECE

FLIP OUT FILTER

HOLDER

LIGHT PATH

SELECTOR LEVER

PHOTO PORT

CONDENSER

TRINOCULAR OBSERVATION HEAD

FOCUS STOP KNOB

LAMP COVER

OBJECTIVE

INTERPUPILLARY

DISTANCE SCALE

Page 4

SLIDE CLAMP

FINE FOCUSING KNOB

MAINS CABLE

ATTACHMENT

FUSE

DIOPTRIC

ADJUSTMENT RING

ON-OFF SWITCH

CONDENSER HEIGHT

ADJUSTMENT (4)

TENSION

ADJUSTMENT KNOB (5)

COARSE FOCUSING KNOB

1.0 DESCRIPTION

Page 5

CONDENSER

TURRET (6) PHASE RINGS

CENTERING LEVERS (7)

1.0 DESCRIPTION

Page 6

Thismicroscopeisascienticprecisioninstrumentdesignedtolastformanyyearswithaminimumofmain-

tenance.Itisbuilttohighopticalandmechanicalstandardsandtowithstanddailyuse.

Optika reminds you that this manual contains important information on safety and maintenance, and that it

must therefore be made accessible to the instrument users.

Optikadeclinesanyresponsibilityderivingfrominstrumentusesthatdonotcomplywiththisma-nual.

2.1 Safety guidelines

Thismanualcontainsimportantinformationandwarningsregardingsafetyaboutinstallation,useand

maintenance of the microscope B-350. Please read this manual carefully before using the equipment.

Toensuresafeuse,theusermustreadandfollowallinstructionsinthismanual.OPTIKAproducts

are designed for safe use in normal operating conditions. The equipment and accessories described

in the manual are manufactured and tested according to industry standards for safety instrumentation

laboratory. Misuse can cause personal injury or damage to the instrument. Keep this manual at hand

close to the instrument, for an easy consultation.

2.2 Electrical safety

Beforeconnectingthepowercordtowalloutlet,ensurethatyourmainsvoltageforyourregioncorre-

spondstothevoltagesupplyoftheinstrument,andthattheilluminator’sswitchisinpositionOFF.The

usermustobservethesafetyregulationsinforceinhisregion.TheinstrumentisequippedwithCEsa-

fety marking, in any case the user has full responsibility concerning the safe use of that instrument.

2.3 Warning/Caution symbols used in this manual

Theusershouldbeawareofsafetyaspectswhenusingtheinstrument.Warningorhazardsymbols

areshownbelow.Thesesymbolsareusedinthismanual.

The instructions on this symbol to avoid possible severe personal injuries.

Warning of use; the incorrect operation on the instrument can cause damages

to the person or instrument.

Possibility of electric shock.

Attention: high temperature surfaces. Avoid direct contact.

Technical notes or usage tips.

2.0 INTRODUCTION

Page 7

3.0 UNPACKING AND ASSEMBLY

The microscope is located in a styrofoam moulded packaging. After removing the adhesive tape from

all packaging, lift the top half of the packaging. Pay attention not to drop or damage the optical com-

ponents(objectivesandeyepieces).Extractthemicroscopefromitspackagingwithbothhands(one

aroundthetoparmandonearoundthebase)andplaceitonastablesurface.Keepitawayfrom

solvents, chemical vapors and excessive moisture. Avoid high temperature environments, the direct

sunshineandexcessivevibrations,whichcouldaffecttheperformanceoftheinstrument.

3.1 Operating environment

Temperature: : 10 - 36°C (50 – 96.8°F)

Relative humidity: 0 – 85% up to 30°C (86°F)

3.2 Unpacking microscope

Control the packaging to ensure that all material is present. We recommend that you take note of all

the accessories to facilitate any future orders of spare parts and technical support calls. Make sure

that in the packaging no small accessories or small parts remain. Please keep the original packaging

in a safe place for future transport needs of microscope or accessories.

 Nevertouchtheglasssurfacessuchaslensesorlters.Tracesofgreaseorotherresiduescanredu-

cethevisionqualityofthenalimageandcorrodethesurfaceoflensesinashorttime.

3.3 Installing the microscope

 Settheopticalheadonthetoparmthroughthelockingscrew.Inserttheeyepiecesintothetubesand

lockthemwiththesmallscrewswhicharelocatedtothesideofthetubes.Removetheprotectivelm

from the stage of the microscope.

3.4 Connect the mains plug into the socket at the base

Page 8

Make sure, before you turn the illumination on, that the voltage selector is set to the mains voltage for

your region.

 Thepowercordshouldbeusedonlyonnetworksocketsequippedwithadequategrounding.

Contact a technician to check the state of your electrical system.

 Ifthereisnoneedtoinstalladditionalaccessories,theinstrumentisnowreadyforuse.

3.0 UNPACKING AND ASSEMBLY

Page 9

Oncepositionedandinstalledwiththenecessarycomponents,themicroscopeisreadytobeused.

Yourmicroscopeisalaboratoryinstrumentdesigned to last. Handle italwayscarefullyandavoid

abrupt vibrations or shocks.

Alwaysdisconnectthepowercablefromthemicroscopewhennotinuseforlongtime,whileyou

cleanitorwhenyouperformanymaintenance.

AVOID DISASSEMBLING THE INSTRUMENT

Donotdisassembletheinstrument.Thisentailsthecancellationofthewarrantyandmaycausemal-

function.

4.1 Observation head

 Loosenthelock-screw(1),turntheobservationheadtoacomfortablepositionforobservation,and

thenlockthelock-screw.

4.2 Place the specimen on the stage

Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the spe¬cimen is

centred over the stage opening by adjusting the coaxial knobs of the stage.

4.3 Illumination system settings

 ThemicroscopeisttedwithawhiteLEDilluminator.Beforeturningontheilluminationsystem,read

thesection5.3aboutelectricalsafetyprecautions.Inserttheplugofthecableintothepowersocket

andturnontheswitchonthebackofthemainbody.Turnthebrightnessadjustmentknobtoabright-

ness suitable for observation.

4.4 Adjust interpupillary distance

 Holdtherightandleftpartsoftheobservationheadwithbothhandsandadjusttheinterpupil¬lary

distancebyturningthetwopartsuntilonecircleoflightcanbeseen.

 Thewhitedot(°)placedontherighteyepieceshowsthesetinterpupillarydistance.Justremember

this value to help on later settings.

4.5 Focus tension adjustment

The tension of the coarse focusing knob is preset by factory. To change the tension according to your

preference,justrotatetheknob(5)clockwiseinordertoincreaseit.

Excessive tension could damage the mechanism of focus. A too loosed tension causes the descent

of the stage by gravity or a sudden loss of focus. In this case, rotate the knob (5) to increase the ten-

sion.

4.0 USING THE MICROSCOPE

Page 10

4.6 Diopter adjustment

Turnthedioptricadjustmentringontherighteyepiecedioptricuptoalignthebottomwiththegradua-

tedring.Turnthecoarsefocusknobinordertofocustheslidewithanobjectivewithlowmagnica-

tion.Adjustthenefocusknobuntilyouobtainaclearanddenedpictureobservingwiththeright

eye,andthenrepeattheoperationwiththeleftdioptriccompensationringandthelefteye.Whenthe

imageappearsinfocus,choosethenecessaryobjectivewiththerevolvingnosepiece.

4.7 Condenser

Raiseorlowerthecondenserthroughtheknob(4)toobtainaclearanduniformilluminationofthe

sample.

Tocenterthecondenser:completelyclosetheirisdiaphragm(3).Usingthecondensercenteringscrews

(2),movethediaphragminthecenteroftheeldofview.Thengraduallyexpandthediaphragmuntil

itistangenttotheedgesoftheeldofview.Ifnecessary,youcanperformanadditionaladjustment.

Thecondenseriscenteredwhentheedgesofirisdiaphragmaretangenttotheeldofview.

4.8 Numerical aperture setting

The value of the numerical aperture (N.A.) of the diaphragm is an indication of the contrast of the illu-

minationsystem.Matchingthevalueofilluminationsystem’sN.A.withthatoftheobjectiveensures

the best results in terms of contrast and image quality. To set the numerical aperture of the Illuminator,

adjusttheopeningoftheirisdiaphragm(3).Inthiswayyoucontrolcontrastandimageresolution.For

sampleswithlowcontrastsettheiristoabout75%ofthevalueoftheobjective’snumericalapertu-

re.

4.9 Phase rings centering (models B-352Ph and B353Ph)

Formodelsequippedwithphasecontrastset,youhavetoperformthecenteringofthephaserings.

Remove an eyepiece from the head and insert the centering telescope in the empty tube.

4.0 USING THE MICROSCOPE

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