Optika B-380 Series User manual

OPTIKA MICROSCOPES - ITALY

www.optikamicroscopes.com - [email protected]

Ver. 2.0.0

OPERATION MANUAL

GUIDA UTENTE

MANUEL D’INSTRUCTIONS

MANUAL DE INSTRUCCIONES

BEDIENUNGSANLEITUNG

B-380 - B-380ALC

Page 2

INDEX

1.0 DESCRIPTION pag. 3

2.0 INTRODUCTION pag. 5

3.0 UNPACKING AND ASSEMBLY pag. 6

4.0 USING THE MICROSCOPE pag. 7

4.1 Observation head

4.2 Place the specimen on the stage

4.3 Illumination system settings

4.4 Interpupillary distance

4.5 Focus tension adjustment

4.6 Diopter adjustment

4.7 Condenser

4.8 Numerical aperture setting

4.9 Phase rings centering (models B-380PH)

5.0 AUTOMATIC LIGHT CONTROL (B-382-ALC MODELS)

pag. 10

6.0 MAINTENANCE

pag. 11

6.1 Microscopy environment

6.2 Before and after using the microscope

6.3 Precautions for a safe use

6.4 Cleaning the optics

7.0 RECOVERY AND RECYCLING pag. 12

Page 3

1.0 DESCRIPTION

PHOTO/VIDEO PORT

HEAD LOCKING

SCREW (1)

REVOLVING NOSEPIECE

STAGE

TRANSLATION KNOBS

BRIGHTNESS

ADJUSTMENT

(LEFT SIDE)

CONDENSER CENTERING

SCREWS (2)

IRIS DIAPHRAGM (3)

EYEPIECE

CONDENSER

LED ILLUMINATOR

OBJECTIVE

DIOPTRIC

ADJUSTMENT RING

Page 4

SLIDE CLAMP

FINE FOCUSING KNOB

CONDENSER HEIGHT

ADJUSTMENT (4)

TENSION

ADJUSTMENT KNOB (5)

COARSE FOCUSING KNOB

1.0 DESCRIPTION

FOCUS STOP KNOB

INTERPUPILLARY DISTANCE

Page 5

This microscope is a scientic precision instrument designed to last for many years with a minimum of main-

tenance. It is built to high optical and mechanical standards and to withstand daily use.

Optika reminds you that this manual contains important information on safety and maintenance, and that it

must therefore be made accessible to the instrument users.

Optika declines any responsibility deriving from instrument uses that do not comply with this ma-nual.

2.1 Safety guidelines

This manual contains important information and warnings regarding safety about installation, use and

maintenance of the microscope B-380. Please read this manual carefully before using the equipment.

To ensure safe use, the user must read and follow all instructions in this manual. OPTIKA products

are designed for safe use in normal operating conditions. The equipment and accessories described

in the manual are manufactured and tested according to industry standards for safety instrumentation

laboratory. Misuse can cause personal injury or damage to the instrument. Keep this manual at hand

close to the instrument, for an easy consultation.

2.2 Electrical safety

Before connecting the power cord to wall outlet, ensure that your mains voltage for your region corre-

sponds to the voltage supply of the instrument, and that the illuminator’s switch is in position OFF. The

user must observe the safety regulations in force in his region. The instrument is equipped with CE

safety marking, in any case the user has full responsibility concerning the safe use of that instrument.

2.3 Warning/Caution symbols used in this manual

The user should be aware of safety aspects when using the instrument. Warning or hazard symbols

are shown below. These symbols are used in this manual.

The instructions on this symbol to avoid possible severe personal injuries.

Warning of use; the incorrect operation on the instrument can cause damages

to the person or instrument.

Possibility of electric shock.

Attention: high temperature surfaces. Avoid direct contact.

Technical notes or usage tips.

2.0 INTRODUCTION

Page 6

3.0 UNPACKING AND ASSEMBLY

The microscope is located in a styrofoam moulded packaging. After removing the adhesive tape from

all packaging, lift the top half of the packaging. Pay attention not to drop or damage the optical com-

ponents (objectives and eyepieces). Extract the microscope from its packaging with both hands (one

around the top arm and one around the base) and place it on a stable surface. Keep it away from

solvents, chemical vapors and excessive moisture. Avoid high temperature environments, the direct

sunshine and excessive vibrations, which could affect the performance of the instrument.

3.1 Operating environment

Temperature: : 10 - 36°C (50 – 96.8°F)

Relative humidity: 0 – 85% up to 30°C (86°F)

3.2 Unpacking microscope

Control the packaging to ensure that all material is present. We recommend that you take note of all

the accessories to facilitate any future orders of spare parts and technical support calls. Make sure

that in the packaging no small accessories or small parts remain. Please keep the original packaging

in a safe place for future transport needs of microscope or accessories.

Never touch the glass surfaces such as lenses or lters. Traces of grease or other residues can re-

duce the vision quality of the nal image and corrode the surface of lenses in a short time.

3.3 Installing the microscope

Set the optical head on the top arm through the locking screw. Insert the eyepieces into the tubes and

lock them with the small screws which are located to the side of the tubes. Remove the protective lm

from the stage of the microscope.

3.4 Connect the external power supply into the socket at the base

Page 7

Once positioned and installed with the necessary components, the microscope is ready to be used.

Your microscope is a laboratory instrument designed to last. Handle it always carefully and avoid

abrupt vibrations or shocks.

Always disconnect the power cable from the microscope when not in use for long time, while you

clean it or when you perform any maintenance.

AVOID DISASSEMBLING THE INSTRUMENT

Do not disassemble the instrument. This entails the cancellation of the warranty and may cause mal-

function.

4.1 Observation head

Loosen the lock-screw (1), turn the observation head to a comfortable position for observation, and

then lock the lock-screw.

4.2 Place the specimen on the stage

Lock the specimen slide on the mechanical stage using the slide clamp. Ensure that the specimen is

centred over the stage opening by adjusting the coaxial knobs of the stage.

4.3 Illumination system settings

The microscope is tted with a white LED illuminator. Before turning on the illumination system, read

the section 5.3 about electrical safety precautions. Insert the plug of the cable into the power socket

and turn on the switch on the side of the main body. Turn the brightness adjustment knob to a bright-

ness suitable for observation.

4.4 Adjust interpupillary distance

Hold the right and left parts of the observation head with both hands and adjust the interpupillary dis-

tance by turning the two parts until one circle of light can be seen.

The white dot (°) placed on the left eyepiece shows the set interpupillary distance. Just remember this

value to help on later settings.

4.5 Focus tension adjustment

The tension of the coarse focusing knob is preset by factory. To change the tension according to your

preference, just rotate the knob (5) clockwise in order to increase it.

Excessive tension could damage the mechanism of focus. A too loosed tension causes the descent of

the stage by gravity or a sudden loss of focus. In this case, rotate the knob (5) to increase the tension.

4.0 USING THE MICROSCOPE

Page 8

4.6 Diopter adjustment

Turn the dioptric adjustment ring on the left eyepiece to the zero position. Turn the coarse focus knob

in order to focus the slide with an objective with low magnication. Adjust the ne focus knob until you

obtain a clear and dened picture observing with the right eye, and then act on the left dioptric com-

pensation ring observing with the left eye. When the image appears in focus, choose the necessary

objective with the revolving nosepiece.

4.7 Condenser

Raise or lower the condenser through the knob (4) to obtain a clear and uniform illumination of the

sample.

To center the condenser: completely close the iris diaphragm (3). Using the condenser centering

screws (2), move the diaphragm in the center of the eld of view. Then gradually expand the dia-

phragm until it is tangent to the edges of the eld of view. If necessary, you can perform an additional

adjustment.

The condenser is centered when the edges of iris diaphragm are tangent to the eld of view.

4.8 Numerical aperture setting

The value of the numerical aperture (N.A.) of the diaphragm is an indication of the contrast of the il-

lumination system. Matching the value of illumination system’s N.A. with that of the objective ensures

the best results in terms of contrast and image quality. To set the numerical aperture of the Illuminator,

adjust the opening of the iris diaphragm (3). In this way you control contrast and image resolution. For

samples with low contrast set the iris to about 75% of the value of the objective’s numerical aperture.

4.9 Phase rings centering (models B-380PH)

For models equipped with phase contrast set, you have to perform the centering of the phase rings.

Remove an eyepiece from the head and insert the centering telescope in the empty tube.

4.0 USING THE MICROSCOPE

Page 9

Insert the 10x objective rotating the nosepiece.

Rotate the turret of the condenser until you reach the inscription “10”.

Loosening the lock screw of the centering telescope, focus on the light ring that you observe.

Rotate the two centering screws in order to move the bright ring until it is perfectly aligned with the

dark ring.

Repeat for the other objectives (only as a verication of the correct centering).

Phase rings will be centered when you see an image like this:

4.0 USING THE MICROSCOPE

Page 10

B-382-ALC models have a special function called “Automatic Light Control”.

The level of light is adjusted by the microscope in order to maintain the same level as the one the user

has chosen, no matter if the aperture of the diaphragm changes, another objective is inserted, opacity

of the sample changes, etc.

1) Set the focus on the sample as described in the previous chapters, using the objective of your

choice.

2) Rotate the brightness adjustment knob in order to get a comfortable level of illumination. NOTE:

ALC system works properly with medium-to-high light intensity, do not set the brightness to a minimum

level.

3) Press the ALC button on the side of the microscope.

Now the ALC system is working: if you open or close the aperture diaphragm or change objective, the

system will try to maintain the same level of illumination as the one you stored when you pressed the

button.

4) Press the ALC button again to return to a manual control of illumination.

5.0 AUTOMATIC LIGHT CONTROL (B-382-ALC MODELS)

BRIGHTNESS

ADJUSTMENT KNOB

ALC BUTTON

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