Optika B-1000 POL User manual

Version: 1

Issued: 25, 02, 2015

Model

B-1000POL

B-1000 Series

INSTRUCTION MANUAL

Page 2

Table of Contents

Warning

Symbols and conventions

Safety Information

Intended use

List of accessories and spare parts

Overview

Unpacking

Assembling

Using the microscope

Maintenance

Troubleshooting

Equipment disposal

Page 3

Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of mainte-

nance. It is built to high optical and mechanical standards and to withstand daily use. We remind you that this

manual contains important information on safety and maintenance, and that it must therefore be made acces-

sible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses that does

not comply with this manual.

Symbols and conventions

The following chart is an illustrated glossary of the symbols that are used in this manual.

CAUTION

This symbol indicates a potential risk and alerts you to proceed with caution.

ELECTRICAL SHOCK

This symbol indicates a risk of electrical shock.

Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the

operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety

regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibil-

ity to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure

safe operation of the unit.

Intended use

For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4

List of accessories and spare parts

CAT. NO. DESCRIPTION

M-1001 WF10x/22mm eyepiece (pair)

M-1002 WF10x/24mm eyepiece (pair)

M-1004.N Centering telescope, 30mm diameter

M-781 WF10x/22mm micrometer eyepiece (10mm, 0.1mm div.)

M-1011 Trinocular Head (3 positions)

M-1012 Binocular ERGO head

M-1033 Bertrand Lens with Analyzer and slot for slides (with Lambda, 1/4 Lambda and Quartz Edge)

M-1044 Quintuple nosepiece with centrable positions for POL objectives

M-1080 4x IOS POL PLAN objective

M-1081 10x IOS POL PLAN objective

M-1082 40x IOS POL PLAN objective

M-1083 60x IOS POL PLAN objective

M-1145 Rotating Stage, centrable

M-1146 Attachable mechanical stage for rotating Stage

M-005 Micrometric slide, 26x76mm, range 1mm, div. 0,01mm

M-690 Eyecup (pair)

M-619 Photo tube adapter for full frame SLR camera

M-173 Photo tube adapter for APS-C SLR camera

M-699 Photo tube adapter for DIGI digital camera series

M-620 CCD camera adapter for 1/3’’ sensor

M-620.1 CCD camera adapter for ½’’ sensor

M-114 CCD camera adapter 0,45x

M-113.1 Ring adapter, 30mm (for monocular and binocular microscopes)

M-617.1N Phase contrast set with IOS PLAN objectives 40x

Page 5

Overview

STAGE LOCK

KNOB

COARSE

FOCUSING

KNOB

NOSEPIECE

DIOPTRIC

ADJUSTMENT

RING

PHOTO PORT

MAIN ON/OFF

EYEPIECE

ANALYZER

FILTER

BERTRAND LENS

DISC

CONDENSER

CENTERING

KNOBS

APERTURE

DIAPHRAGM

POLARIZER

FIELD DIAPHRAGM

BRIGHTNESS

ADJUSTMENT KNOB

FOCUS-STOP KNOB

FINE FOCUSING

KNOB

OBJECTIVE

STAGE

FILTER

PLATE HOLDER

Page 6

Put the microscope stand on a

solid table.

First insert the Bertrand lens

attachment, use the 2mm

Allen wrench to tighten the

screw.

All screws are already inser-

ted into each threaded hole.

(Fig.1)

Unpacking

The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container

and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)

fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope

from the container and put it on a stable desk.

Assembling

Once you open the box, these are the microscope’s components:

① Condenser

② Optical head

③ Main body

④ Eyepieces

⑤ Power supply

⑥ Stage

⑦ Objectives

⑧ Bertrand lens and analyzer

⑨ Retardation plates

①

②

③

④

⑤

⑥

⑦

⑧ ⑨

Fig.1

Page 7

Insert the condenser under

the stage: position until it is

well inserted into its holder

(under the condenser there is

a pin that must fully enter the

guide of the holder). (Fig. 4)

AFTER INSERTING THE

CONDENSER, TIGHTEN

THIS SCREW

Insert the optical head above

the Bertrand lens, using the

other 3mm Allen wrench to

tighten the screw. (Fig.2)

Insert both eyepieces into

the tubes of the optical head.

(Fig.3)

SPRING

Mount the rotating stage: at

the bottom of the stage there

is a spring, push this spring

toward the stage support ①,

then push the stage

downward ②. (Fig.5)

Fig.2

Fig.3

Fig.4

Fig.5

①

②

Page 8

Insert the retardation plates

by sliding them into the slot

under the optical head. (Fig.7)

Center each objective using

the provided screwdrivers

(see chapter using the micro-

scope). (Fig.8)

Insert the power supply jack

on the connector at the rear.

(Fig.9)

Screw each objective into the

thread of the nosepiece, in

order of magnication. (Fig.6)

Fig.6

Fig.7

Fig.8

Fig.9

Page 9

The rotating stage is provided

with:

a) Stage centering knobs;

b) Stage lock knob;

c) 45° rotation clicks;

d) Optional X-Y translator.

(Fig.10)

The Bertrand lens attachment

is provided with

a) Analyzer lter;

b) Retardation plates;

c) Lens focus adjustment;

d) Lens X-Y centering screws.

(Fig.11)

X-Y TRANSLATION KNOBS

STAGE CENTERING KNOBS

BERTRAND LENS ATTACHMENT

RETARDATION PLATES

Fig.10

Fig.11

Page 10

B-1000 illumination can be managed through

the keyboard placed on the left of the stand:

1) ON-OFF: press this button to turn on or off

the LED illuminator.

2) BOOST: press this button in order to incre-

ase the brightness (useful for high-magnica-

tion objectives or very opaque specimens).

2) AUTO-OFF: if you want the illuminator to

switch off automatically, press this button until

15, 30 or 60 minutes delay is set. After this

period of time, the light will turn off. You have

to press the ON-OFF button to turn it on again.

(Fig.12)

In order to activate the transmitted light illu-

minator, insert the plug of the external power

supply into the socket and turn on the main

switch on the side of the main body. Press

the ON-OFF button on the control keyboard

and turn the brightness adjustment knob to a

brightness suitable for observation. (Fig.13)

Do not enable boost mode while observing with

low magnication objectives (4x, 10x) with fully

open diaphgram: the high brightness may hurt

user’s eyes.

Control keyboard

LED settings

Preliminary settings

1) ON-OFF

3) AUTO-OFF

2) BOOST

MAIN

SWITCH

• The eld diaphragm is

fully open. (Fig.14)

• The aperture diaphragm

is fully open. (Fig.15)

• No lter is inserted under

the observation head.

Using the microscope

Fig.12

Fig.13

Fig.14

Fig.15

Before trying to focus the

sample, ensure that:

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