Optika B-510 Series User manual

Ver. 3.2 2022

INSTRUCTION MANUAL

B-510 Series

Model

B-510BF

B-510ERGO

B-510ASB

B-510PH

B-510FL

B-510LD4

B-510LD4-SA

B-510LD4D

B-510-2

B-510-2F

B-510-3

B-510-5

Page 2

Table of contents

1. Warning 4

2. Safety Information 4

3. Package content 5

3.1 B-510BF/B-510ERGO 5

3.2 B-510PH 6

3.3 B-510ASB 7

3.4 B-510-2/B-510-2F/B-510-3/B-510-5 8

3.5 B-510FL 9

3.6 B-510LD4/B-510LD4-SA 10

3.7 B-510LD4D 11

4. Unpacking 12

5. Intended use 12

6. Symbols and conventions 12

7. Instrument description 13

7.1 B-510BF/B-510ERGO 13

7.2 B-510PH 15

7.3 B-510ASB 17

7.4 B-510-2/B-510-2F/B-510-3/B-510-5 18

7.5 B-510FL 19

7.6 B-510LD4/B-510LD4-SA 20

7.7 B-510LD4D 21

8. Assembling 23

8.1 B-510BF/ERGO/PH/ASB 23

8.2 B-510-2/B-510-2F/B-510-3/B-510-5 24

8.3 B-510FL 27

8.4 B-510LD4/B-510LD4-SA 29

8.5 B-510LD4D 30

8.6 Installing the PC screen (B-510LD4D) 30

8.7 Cable connection (B-510LD4D) 30

8.8 Installing uorescence lter (B-510FL) 32

8.9 Installing uorescence lter (B-510LD series) 35

8.10 Replacing uorescence lter (B-510LD series) 36

8.11 Polarizing set (optional) 37

9. Brightfield observation procedures (B-510BF/B-510ERGO) 38

10. Use of the microscope (B-510BF/B-510ERGO/B-510-2-2F-3-5) 39

10.1 Light intensity adjustment 39

10.2 Coarse focus tension adjustment 39

10.3

Coarse upper limit lever 39

10.4 Stage 40

10.5

Diopter adjustment 40

10.6 Adjusting the interpupillary distance 40

10.7 Light path selection (only uorescence models) 40

10.8 Use of eye shields 41

10.9 Centering the condenser 41

10.10 Effects of the eld diaphragm 41

10.11 Aperture diaphragm 42

10.12 Use of oil immersion objective 42

10.13 Use of the pointer (B-510-2/2F/3/5) 43

10.14 Use of the polarizer (optional) 43

11. Condenser for Brightfield/Darkfield/Phase Contrast (B-510PH) 44

11.1 Brighteld Observation (BF) 44

11.2 Darkeld Observation (DF) 44

11.3 Phase Contrast Observation (PH) 45

11.4 Use of the green lter 46

12. Condenser for Brightfield/Phase Contrast (B-510ASB) 46

12.1 Brighteld Observation (BF) 47

12.2 Phase Contrast Observation (PH) 47

13. Fluorescence observation procedures (B-510FL) 48

14. Fluorescence observation procedures (B-510LD series) 48

15. Use of the microscope (B-510FL/B-510LD4/B-510LD4-SA/B-510LD4D) 49

15.1 Microscope setting (B-510FL) 49

15.2 Use of the microscope (B-510FL) 51

15.2.1 Use of the shutter (B-510FL only) 51

Page 3

15.3 Use of the microscope (B-510LD series) 52

15.4 Use of light excluding plate 53

15.5 Use of UV shield 53

16. Simultaneous observation in Phase Contrast + Fluorescence 54

16.1 B-510FL 54

16.2 B-510LD series 54

17. Microphotography 55

17.1 Use of C-mount cameras 55

17.2 Use of reex cameras 55

18. Maintenance 56

19. Troubleshooting 57

Equipment disposal 59

Page 4

1. Warning

This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It

is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains

important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.

We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.

2. Safety Information

Avoiding Electrical Shock

Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage

of the equipment and that the lamp switch is in off position. Users should observe all safety regulations of the region. The

equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please

follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 5

① Microscopeframe

② Eyepieces

③ Objectives

④ Observationhead

⑤ Immersionoil

⑥ Allenwrench

⑦ Tensionadjustmenttool

⑧ Dustcover

⑨ Powersupply

③

⑤

⑥

⑦

⑧

⑨

①

②

④

3. Package content

3.1 B-510BF/B-510ERGO

Page 6

① Microscopeframe

② Eyepieces

③ Objectives

④ Observationhead

⑤ Centeringtelescope

⑥ Immersionoil

⑦ Allenwrench

⑧ Tensionadjustmenttool

⑨ Dustcover

⑩ Greenlter

⑪ Powersupply

①

②

③④

⑤

⑥

⑦

⑧

⑨

⑩

⑪

3.2 B-510PH

Page 7

① Microscopeframe

② Eyepieces

• 10x(onepair)

• 12,5x(onepair)

③ Objectives

④ Observationhead

⑤ Centeringtelescope

⑥ Immersionoil

⑦ Allenwrench

⑧ Tensionadjustmenttool

⑨ Dustcover

⑩ Greenlter

⑪ Powersupply

①

②

③④

⑤

⑥

⑦

⑧

⑨

⑩

⑪

3.3 B-510ASB

Page 8

①

②

③

④

⑤

⑥

⑦

⑧

⑩

⑨

① Microscopeframe

② Eyepieces

• 10x/22(onepairformainhead)

• 10x/20(onepairforB-510-2/2F)

• 10x/20(twopairsforB-510-3)

• 10x/20(fourpairsforB-510-5)

③ Objectives

④ Mainobservationhead

⑤ Sideobservationheads

• oneforB-510-2/2F

• twoforB-510-3

• fourforB-510-5

⑥ Immersionoil

⑦ Allenwrench

⑧ Tensionadjustmenttool

⑨ Dustcover

⑩ Powersupply

②

3.4 B-510-2/B-510-2F/B-510-3/B-510-5

Page 9

⑪

⑫

① Microscopeframe

② Eyepieces

③ Objectives

④ Observationhead

⑤ Lamphousing

⑥ Epi-illuminator

⑦ HBObulbs

⑧ Allenwrench

⑨ Tensionadjustmenttool

⑩ Dustcover

⑪ UVprotectionshield

⑫ Powersupply

⑬ Powercord

⑭ Fluorescencepowersupply

⑮ Lightexcludingplate

⑯ Immersionoil

①

②

③

④

⑤

⑥

⑦

⑧⑨

⑩

⑬

⑭

⑮

⑯

3.5 B-510FL

Page 10

① Microscopeframe

② Eyepieces

③ Objectives

• B-510LD4:W-PLANseries

• B-510LD4-SA:W-PLANFseries

④ Observationhead

⑤ Epi-illuminator

⑥ Allenwrench

⑦ Tensionadjustmenttool

⑧ Immersionoil

⑨ Dustcover

⑩ Powersupply

• 6Vfortransmittedlight

• 12Vforuorescence

⑪ Lightexcludingplate

⑫ UVprotectionshield

①

②

③

④

⑤

⑥

⑦

⑧

⑨

⑩

⑪⑫

⑤

3.6 B-510LD4/B-510LD4-SA

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