PSI PolyPen RP400 Manual

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Manual Version: 2022/01

www.psi.cz

This document and its parts can be copied or provided to a third party only with the express permission of PSI.

The contents of this manual have been verified to correspond to the specifications of the device. However, deviations cannot be ruled out.

Therefore, a complete correspondence between the manual and the real device cannot be guaranteed. The information in this manual is

regularly checked, and corrections may be made in subsequent versions.

The visualizations shown in this manual are only illustrative.

This manual is an integral part of the purchase and delivery of equipment and its accessories and both Parties must abide by it.

 
 
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TABLE OF CONTENT 
 
1Information before using PolyPen device ......................................................................................................... 4 
2General Description ......................................................................................................................................... 5 
2.1 Technical Specification..............................................................................................................................................7
3Device Description ........................................................................................................................................... 8 
3.1 List of equipment and customer information..........................................................................................................10 
3.2 Care and maintenance............................................................................................................................................10 
4Getting started............................................................................................................................................... 11 
4.1 Calibration ..............................................................................................................................................................11 
4.2 Measurement .........................................................................................................................................................12 
5Menu description........................................................................................................................................... 13 
6PC communication ......................................................................................................................................... 17 
6.1 USB connection.......................................................................................................................................................17 
6.2 Driver installation ...................................................................................................................................................17 
7SpectraPen software...................................................................................................................................... 20 
7.1 Software installation...............................................................................................................................................20 
7.2 Software menu .......................................................................................................................................................22 
7.3 Data transfer and visualization...............................................................................................................................23 
7.4 Online control .........................................................................................................................................................27 
7.5 Firmware update ....................................................................................................................................................28 
8GPS module ................................................................................................................................................... 29 
8.1 GPS/PolyPen operation...........................................................................................................................................29 
9Warranty terms and conditions...................................................................................................................... 31 
10 Troubleshooting and Customer Support......................................................................................................... 31 
11 Appendix ....................................................................................................................................................... 32 
11.1 Programming custom index in PolyPen ..................................................................................................................32 
11.1.1 Function description...............................................................................................................................................33 
 
 

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1INFORMATION BEFORE USING POLYPEN DEVICE

Read this manual carefully before operating the device. If you are not sure about something in the manual, contact the manufacturer for

clarification.

By accepting the device, the customer agrees to follow the instructions in this guide.

Always follow corresponding manuals while working with the PolyPen device or doing the maintenance.

It is forbidden to interfere with the hardware or software of the PolyPen device in any way without previous agreement with the

manufacturer.

The following table presents basic highlight symbols used in this manual:

Symbol

Description

Important information, read carefully.

Complementary and additional information.

Tab. 1 Used symbols.

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2GENERAL DESCRIPTION

PolyPen RP400 & RP410 (in further text - PolyPen) is a portable, handheld spectro-radiometer. The PolyPen features a complete system

primarily designed for a measurement of spectral reflectance of leaves or other planar samples. The system is convenient for both, indoor

as well as field applications.

The PolyPen is a self-contained instrument powered by a Lithium-ion battery which is rechargeable via a PC and a USB cable. However,

it does not require a PC for operation. Reflectance spectra and calculated reflectance indices are instantly displayed on the PolyPen touch

screen and all stored in the device internal memory. Integrated USB communication allows data transfer to a PC at a later time. Measured

spectra and calculated indices are stored as full spectrum and individual values (respectively).

The PolyPen device integrates an internal light source (Xenon incandescent lamp) with radiation range 380 –1050 nm. Two versions of

detectors are available for UV/VIS and NIR spectral regions.

The built-in GPS module and splash-proof case are ideal for field use of the PolyPen and make this device well suited for wide scope of

environmental, agricultural and ecological applications such as monitoring of artificial lighting used in horticulture industry or light source

testing.

PolyPen versions:

PolyPen RP 410 UVIS

Spectral response range: 380 to 790 nm.

PolyPen RP 410 NIR

Spectral response range: 640 to 1050 nm.

PolyPen measures:

Transmittance –calculated using the following formula

T= I/I0

Where: I0is reference light intensity

I is measured light intensity

Absorbance –calculated using the following formula

A=log (I0/I)

Where: I0is reference light intensity

I is measured light intensity

The PolyPen device incorporates formulas of commonly used reflectance indices (e.g. NDVI, NDGI, PRI etc.) directly in its software and

displays values of the selected indices instantly. The PolyPen allow calculation of the following pre-defined vegetation indices:

Normalized Difference Vegetation Index (NDVI)▪

Reference: Rouse et al. (1974)

Equation: NDVI = (RNIR - RRED ) / (RNIR + RRED )

Simple Ratio Index (SR)▪

Reference: Jordan (1969); Rouse et al. (1974)

Equation: SR = RNIR / RRED

Modified Chlorophyll Absorption in Reflectance Index (MCARI1)

Reference: Haboudane et al. (2004)

Equation: MCARI1 = 1.2 * [2.5 * (R790- R670) - 1.3 * (R790- R550)]

Optimized Soil-Adjusted Vegetation Index (OSAVI)▪

Reference: Rondeaux et al. (1996) )

Equation: OSAVI = (1 + 0.16) * (R790- R670) / (R790- R670 + 0.16)

Greenness Index (G)

Equation: G = R554 / R677

Modified Chlorophyll Absorption in Reflectance Index (MCARI)

Reference: Daughtry et al. (2000)

Equation: MCARI = [(R700- R670) - 0.2 * (R700- R550)] * (R700/ R670)

Transformed CAR Index (TCARI)

Reference: Haboudane et al. (2002)

Page | 6

Equation: TSARI = 3 * [(R700- R670) - 0.2 * (R700- R550) * (R700/ R670)]

Triangular Vegetation Index (TVI)

Reference:Broge and Leblanc (2000)

Equation: TVI = 0.5 * [120 * (R750- R550) - 200 * (R670- R550)]

Zarco-Tejada & Miller Index (ZMI)▪

Reference: Zarco-Tejada et al. (2001)

Equation: ZMI = R750 / R710

Simple Ratio Pigment Index (SRPI)

Reference: Peñuelas et al. (1995)

Equation: SRPI = R430 / R680

Normalized Phaeophytinization Index (NPQI)

Reference: Barnes et al. (1992)

Equation: NPQI = (R415- R435) / (R415+ R435)

Photochemical Reflectance Index (PRI)

Reference: Gamon et al. (1992)

Equation: PRI = (R531- R570) / (R531+ R570)

Normalized Pigment Chlorophyll Index (NPCI)

Reference: Peñuelas et al. (1994)

Equation: NPCI = (R680- R430) / (R680+ R430)

Carter Indices

Reference: Carter (1994), Carter et al. (1996)

Equation: Ctr1 = R695 / R420; Ctr2▪ = R695 / R760

Lichtenthaler Indices

Reference: Lichtenthaler et al. (1996)

Equation: Lic1▪ = (R790 - R680) / (R790 + R680); Lic2 = R440 / R690

Structure Intensive Pigment Index (SIPI)

Reference: Peñuelas et al. (1995)

Equation: SIPI = (R790- R450) / (R790+ R650)

Gitelson and Merzlyak Indices

Reference: Gitelson & Merzlyak (1997)

Equation: GM1 = R750/ R550; GM2 ▪= R750/ R700

Anthocyanin Reflectance Indices (ARI1; ARI2)

Reference: Gitelson et al. (2001)

Equation: ARI1 = 1/R550-1/R700; ARI2 = R790*(1/R550-1/R700)

Carotenoid Reflectance Indices (CRI1; CRI2)

Reference: Gitelson et al. (2002)

Equation: CRI1 = 1/R510-1/R550; CRI2 = 1/R510-1/R700

Renormalized Difference Vegetation Index (RDVI)

Reference: Roujean & Breon (1995)

Equation: RDVI = (R780-R670)/((R780+R670)^0.5)

▪indices measured also by RP 410 NIR

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2.1 TECHNICAL SPECIFICATION

Spectral range

380 nm - 790 nm (RP 410 UVIS)

640 nm - 1050 nm (RP 410 NIR)

Spectral response half width

8 nm

Spectral straylight

-30 dB

Scanning speed

About 100 ms

Dynamic range

High gain: 1:4300

Low gain: 1:13000

Size of aperture

7 mm

Light source

Xenon incandescent lamp

Light source spectral range

380-1050 nm

Internal memory capacity

Up to 16 Mbit

Internal data logging

Up to 4,000 measurements

Data transfer

USB cable

PC software

SpectraPen 1.1 (Windows 7 and higher)

Battery type

Li-Ion rechargeable battery

Battery capacity

2600 mAh

Max. charging current

0.5 A

Charging

Via USB port - PC, power bank, USB charger, etc.

Battery life

48 hours typical with full operation

Low battery indicator

Sample holder

Mechanical leaf clip

Display

Touchscreen 240 x 320 pixel; 65535 colors

Built in GPS module

Ultra-high sensitivity down to -165dBm

High accuracy of <1.5 m in 50% of trials

Size

180 x 75 x 40 mm

Weight

300 g

Operating conditions

Temperature: 0 to +55 °C

Relative humidity: 0 to 95 % (non-condensing)

Storage conditions

Temperature: -10 to +60 °C

Relative humidity: 0 to 95 % (non-condensing)

Warranty

1 year parts and labor

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3DEVICE DESCRIPTION

Fig. 1 Device description.

Page | 9

Leaf clip

PolyPen is equipped with a non-destructive leaf-clip sample holder.

Colour touch screen display

PolyPen is operated via a touch screen by using a supplied stylus. The software operation instructions are available on page 20Error!

Bookmark not defined..

No sharp objects should be used when operating the PolyPen via touch screen. It is recommended to use

provided stylus when making selections on the touch screen.

USB Connector

USB connector is used for operation of the PolyPen device directly from PC via the SpectraPen software and for re-charging the device

batteries. Please note that PolyPen device is automatically re-charged when connected to the PC via the USB connector.

Power button

Power button is the main Switch ON/OFF button.

Optical sensor and light source

The optical sensor of the PolyPen and the light source, are placed inside of the device. See schematic drawing of the principle of

reflectance measurement in PolyPen in Fig. 2. The light source is oriented in 35° to the normal line intersecting the detector.

The sensor should be always dust and dirt free.

Fig. 2 Schema of reflectance measurement.

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3.1 LIST OF EQUIPMENT AND CUSTOMER INFORMATION

Standard version of the PolyPen device package consists:

•PolyPen

•Reflectance standard (Spectralon®)

•Stylus

•Carrying Case

•Textile Strap for Comfortable Wearing

•PolyPen Operating Manual (on a USB flash disc)

•SpectraPen software and driver (on a USB flash disc)

•USB cable

•Other Accessories or Optional Features (according to your specific order)

For data download via USB connection, the USB driver needs to be installed on the PC. It can be found on the

installation disk (USB driver folder).

If any item is missing, please, contact the manufacturer. Also check the carton for any visible external damage. If

any damage is found, notify the carrier and the manufacturer immediately. The carton and all packing materials

should be retained for inspection by the carrier or insurer.

For customer support, please write to: [email protected]

3.2 CARE AND MAINTENANCE

PolyPen device

•Never submerge the device in water!

•The device should not come in contact with any organic solvents, strong acids or bases.

•Keep the optical sensor clean and dry. If cleaning is needed, use soft, non-abrasive tissue.

•Do not use sharp objects for touch screen operation.

Li-ion battery

•Avoid fully discharging of the battery.

•Do not keep the battery at full charge for long periods of time. Allow for it to discharge.

•High temperatures shortens battery life.

•If the battery can no longer be charged, please contact PSI for replacement battery and installation instructions.

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