PSI FluorPen FP 110 Manual

Page | 2

Manual Version: 2021/12

www.psi.cz

This document and its parts can be copied or provided to a third party only with the express permission of PSI.

The contents of this manual have been verified to correspond to the specifications of the device. However, deviations cannot be ruled out.

Therefore, a complete correspondence between the manual and the real device cannot be guaranteed. The information in this manual is

regularly checked, and corrections may be made in subsequent versions.

The visualizations shown in this manual are only illustrative.

This manual is an integral part of the purchase and delivery of equipment and its accessories and both Parties must abide by it.

 
 
Page | 3 
 
 
TABLE OF CONTENT 
 
1Information before using FluorPen device........................................................................................................ 5 
2General Description ......................................................................................................................................... 6 
2.1 Technical Specification..............................................................................................................................................8
3Device Description ......................................................................................................................................... 11 
3.1 List of equipment and customer information..........................................................................................................12 
3.2 Care and maintenance............................................................................................................................................12 
4Principle of measurement.............................................................................................................................. 13 
5Getting started............................................................................................................................................... 15 
5.1 Pulses description and setting ................................................................................................................................15 
5.2 Measurement .........................................................................................................................................................18 
5.3 Protocols explanation .............................................................................................................................................18 
5.3.1 OJIP protocol ..........................................................................................................................................................18 
5.3.2 Non-photochemical quenching (NPQ) protocols....................................................................................................20 
5.3.3 Light curve (LC) protocols .......................................................................................................................................23 
5.4 Multiple measurement ...........................................................................................................................................26 
6Control menu tree.......................................................................................................................................... 27 
7USB Connection ............................................................................................................................................. 35 
8Bluetooth connection..................................................................................................................................... 36 
8.1 Bluetooth pairing....................................................................................................................................................36 
9FluorPen software.......................................................................................................................................... 39 
9.1 Software installation...............................................................................................................................................39 
9.2 Menu and icons explanation...................................................................................................................................40 
9.2.1 Main menu .............................................................................................................................................................40 
9.2.2 Menu settings.........................................................................................................................................................41 
9.2.3 Menu online control...............................................................................................................................................41 
9.3 Data transfer and visualization...............................................................................................................................44 
9.4 Firmware update ....................................................................................................................................................46 
10 GPS module ................................................................................................................................................... 48 
10.1 GPS/FluorPen operation .........................................................................................................................................48 
10.2 Data download .......................................................................................................................................................48 
11 Warranty terms and conditions...................................................................................................................... 50 

 
 
Page | 4 
 
 
Troubleshooting and Customer Support......................................................................................................... 50 
Appendix ....................................................................................................................................................... 51 
1 Battery pack for Monitoring Pen ............................................................................................................................51 
1.1 Standard battery pack ............................................................................................................................................51 
1.2 Extended temperature range battery pack ............................................................................................................52 
2 Installation and operation of the Monitoring Pen MP 100-A/B..............................................................................53 
2.1 Device control.........................................................................................................................................................53 
2.2 Device installation ..................................................................................................................................................55 
3 Detachable leaf clips...............................................................................................................................................55 
 
 

Page | 5

1INFORMATION BEFORE USING FLUORPEN DEVICE

Read this manual carefully before operating the device. If you are not sure about something in the manual, contact the manufacturer for

clarification.

By accepting the device, the customer agrees to follow the instructions in this guide.

Always follow corresponding manuals while working with the FluorPen device or doing the maintenance.

It is forbidden to interfere with the hardware or software of the FluorPen device in any way without previous agreement with the

manufacturer.

The following table presents basic highlight symbols used in this manual:

Symbol

Description

Important information, read carefully.

Complementary and additional information.

Table 1 Used symbols.

Page | 6

2GENERAL DESCRIPTION

FluorPen FP 110 is a portable, battery-powered fluorometer that enables quick and precise measurement of chlorophyll fluorescence

parameters (Ft, QY, NPQ, OJIP, and LC of (QY) in plants. The FluorPen can be used in the laboratory, greenhouse, or in the field, where

data can be mapped precisely to the location with the built in GPS module. With the built-in GPS module, the FluorPen is a great device

for studying photosynthetic activity, stress detection, herbicide testing, or mutant/variety/transgenic screening in the field. Affordable

price and user-friendly two-button operation make the FluorPen a perfect tool for teaching photosynthesis. Because of its rapid

measurement capability and large internal memory, the FluorPen is also an invaluable tool for large plant-screening programs.

PAR-FluorPen FP 110 includes all features of the FluorPen FP 110, and measures the same chlorophyll fluorescence parameters as FP110,

but in addition, the PAR-FluorPen has an integrated Light Meter for direct digital readouts of Photosynthetically Active Radiation (PAR)

in the range from 400 to 700 nm. PAR is measured as Photosynthetic Photon Flux Density (PPFD), which is indicated by units of quanta

(photons) per unit time per unit surface area. The sensor has a uniform response to photons in the 400-700 range. Instant readouts are

provided as average values of 20 measurements. It is recommended to recalibrate the PAR sensor every 2 years.

Monitoring Pen MP 100 is a lightweight, portable and a more durable version of the FluorPen. It is designed for long-term, unattended

monitoring of chlorophyll ﬂuorescence parameters in the field or lab experiments. It features weatherproof construction for use even in

adverse environmental conditions, or in the laboratory /greenhouse long term experiments. It is battery operated (internal or external

battery) and as an option may be used with a solar panel as a power supply.

All measured data are sequentially stored in the internal memory of the FluorPen, PAR-FluorPen or Monitoring Pen all collected data can

be transferred from the devices to the PC computer via both USB and Bluetooth communication -. Comprehensive FluorPen 1.1 software,

included with the device provides data transfer, and visualization protocols.

Unless stated otherwise, the information regarding the FluorPen FP 110 is relevant also to PAR FluorpenFP110

and the Monitoring Pen MP 100.

FluorPen versions:

FluorPen FP 110/S

Equipped with a standard attached leaf-clip.

FluorPen FP 110/D

Adapted for use with detachable leaf-clips; leaf clips sold separately in sets of 10.

FluorPen FP 110/P (Fig. 1c)

Intended for autonomous use in indoor conditions (previously Monitoring Pen-S). It features a

plastic case, measuring probe and thread for tripod attachment.

FluorPen FP 110/X

The “X“ version is mounted with custom-made leaf-clip.

PAR-FluorPen FP 110/S (Fig. 1a)

Same features as the standard FluorPen FP 110/S plus Photosynthetically Active Radiation (PAR)

meter in the range from 400 to 700 nm. equipped with a standard leaf-clip.

PAR-FluorPen FP 110/D (Fig. 1b)

Same features as the FluorPen FP 110/D adapted for use with detachable leaf clips plus

Photosynthetically Active Radiation (PAR) meter in the range from 400 to 700 nm. Leaf clips sold

separately.

PAR-FluorPen FP 110/X

Same features as the PAR-FluorPen FP 110/D but the “X“ version is mounted with custom-made

leaf-clip.

Page | 7

Fig. 1 a) PAR-FluorPen FP 110/S. b) PAR-FluorPen FP 110/D. c) FluorPen FP 110/P.

Monitoring Pen versions:

Monitoring Pen MP 100-E (Fig. 2a)

Monitoring Pen MP 100-E is a modified FluorPen designed for extra durability, battery-powered and intended for autonomous use in

field conditions during extended experiments. It features waterproof metal case, measuring probe, thread for tripod attachment,

external pack with batteries and the FluorPen 1.1 software for data collection and processing.

Monitoring Pen MP 100-A (Fig. 2b)

This is a submersible, battery-powered FluorPen intended for underwater measurements of chlorophyll fluorescence parameters (also

autonomous). It features a waterproof case, measuring probe, and the FluorPen 1.1 software for data collection and processing. External

battery pack with batteries is sold separately. This aquatic version of the monitoring pen is intended for use at maximum water depth of

2 meters. The device is equipped with two buttons that allow direct control of the device (even under water). A customized Version B of

this instrument is also available for use in deeper water (maximum 10 m). There are no control buttons on this version of the device.

Version B is controlled via software and a PC (placed above water). The device is usually fixed in static position under water.

Fig. 2 a) Monitoring Pen MP 100-E. b) Monitoring Pen MP 100-A.

Page | 8

2.1 TECHNICAL SPECIFICATION

FluorPen and PAR-FluorPen

Protocols

Ft –instantaneous chlorophyll fluorescence

Quantum Yield

OJIP

Non-photochemical quenching

Light curve

Photosynthetically Active Radiation (measured as PPFD) –PAR-FluorPen only

LED emitter

Blue (470 nm), other wavelengths on request

Saturating pulse Illumination

Up to 3,000 µmol(photon).m-2.s-1 (adjustable from 10 to 100%)

Actinic Illumination

Adjustable from 10 to 1,000 µmol(photon).m-2.s-1

Measuring Illumination

Up to 0,09 µmol(photon).m-2.s-1 per pulse (adjustable from 10 to 100%)

Detector

PIN photodiode with bandpass filters

Wavelength range from 667 to 750 nm

Internal memory capacity

Up to 16 Mb

Internal data logging

Up to 149,000 measurements (depending on protocol)

Data transfer

USB cable

Bluetooth (transfer up to 3Mbps for distance up to 20m)

PC software

FluorPen 1.1 (Windows 7 and higher)

Battery

Li-Ion rechargeable battery

Capacity 2000 mAh

Max. charging current 0.5 A

Charging via USB port - PC, power bank, USB charger, etc.

48 hours typical with full operation

Low battery indicator

Sample holder

Standard leaf-clip (FP 110/S)

Detachable leaf-clip (FP 110/D)

Probe (FP 110/P)

Display

Graphical display

Keypad

Sealed, 2-key tactile response

Turns off after 5 minutes of no use

Built in GPS module

Ultra-high sensitivity down to -165dBm

High accuracy of <1.5 m in 50% of trials

Size

134 x 65 x 33 mm

Weight

188 g

Operating conditions

Temperature: 0 to +55 °C

Relative humidity: 0 to 95 % (non-condensing)

Storage conditions

Temperature: -10 to +60 °C

Relative humidity: 0 to 95 % (non-condensing)

Warranty

1-year parts and labor

Page | 9

Monitoring Pen

Protocols

Ft –instantaneous chlorophyll fluorescence

Quantum Yield

OJIP

Non-photochemical quenching

Light curve

LED emitter

Blue (470 nm), other wavelengths on request

Saturating pulse Illumination

Up to 3,000 µmol(photon).m-2.s-1 (adjustable from 10 to 100%)

Actinic Illumination

Adjustable from 10 to 1,000 µmol(photon).m-2.s-1

Measuring Illumination

Up to 0,09 µmol(photon).m-2.s-1 per pulse (adjustable from 10 to 100%)

Detector

PIN photodiode with bandpass filters

Wavelength range from 667 to 750 nm

Internal memory capacity

Up to 16 Mb

Internal data logging

Up to 149,000 measurements (depending on protocol)

Data transfer

Serial cable

PC software

FluorPen 1.1 (Windows 7 and higher)

External Battery

Standard battery pack

operating temperature from 10 to 40 °C

rechargeable

Extended temperature range battery pack

operating temperature from -40 to 60 °C

non-rechargeable (spare battery)

Capacity 12Ah

Battery life up to 2 years of operation (1 QY measurement per hour)

Sample holder

Probe

Display

2 x 8 characters LC display

Keypad

Sealed, 2-key tactile response

Turns off after 5 minutes of no use

Built in GPS module

Ultra-high sensitivity down to -165dBm

High accuracy of <1.5 m in 50% of trials

Size

134 x 65 x 33 mm

Weight

188 g

Warranty

1-year parts and labor

Page | 10

Bluetooth module compliance data:

Popular Measuring Instrument manuals by other brands

Suaoki

Suaoki D40 user manual

Elster

Elster BK-G1.6 operating instructions

Endress+Hauser

Endress+Hauser Deltabar FMD71 technical information

Duratool

Duratool D03128 manual

Vega

Vega VEGAPULS 65 Quick setup guide

Tonghui

Tonghui TH2618B Operation manual

Tenmars

Tenmars TM-801 user manual

MSR

MSR 145 user manual

TPS

TPS WP-82 manual

ELMETRON

ELMETRON CP-511 user manual

PCB Piezotronics

PCB Piezotronics 003C30 Installation and operating manual

Tenma

Tenma 72-10465 manual

Endress+Hauser

Endress+Hauser Proline Promag 50 Brief operating instructions

Siemens

Siemens SITRANS F Coriolis FCT030 Function manual

KLINGER

KLINGER CMF V Series instruction manual

EXFO

EXFO FTB-2 Notice

Keysight

Keysight M8290A Getting started guide

ADTEK

ADTEK MW-5 operating manual