MRC SPECTRO-96 User manual
PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION
MRC.VER.01-5.12
Operation Manual for
FLUORESCENT SPECTROPHOTOMETER
SPECTRO-96
1
Preface
The method of fluorescence analysis (fluorometry) is a modern analytical
method of high PMT level and high selectivity. It much can provide information,
including an excitation spectrum, an emission spectrum, fluorescent intensity,
fluorescence life, and fluorescence polarization. The linear range of working
curve is wide and hence it has been an important analytical method in the field of
trace analysis.
The Spectro 96 Fluorospectrophotometer is a fluorophotometer using filters
with a monochromator. It has three functions, namely, wavelength scan, time
scan for fluorescent intensity, and quantitative analysis. The instrument is
capable of performing two modes of measurement: measuring with a single
instrument only, and measuring with an on-line computer. The amounts of the
functions and the convenience of measuring vary with the different modes of
measurement.
For proper use of the Spectro 96 Fluorospectrophotometer, the user of the
instrument should have a basic knowledge of using optical instruments and a
basic knowledge of molecular fluorescence spectroscopy. The user whose
instrument is equipped with a computer also should have a corresponding
knowledge and skills of operating the computer.
Before installation or the first use of this instrument, the user must read
through this instruction manual carefully. According to the user’s different
equipment, the spectrophotometer main unit may be equipped with an external
computer or a special printer.
Our company guarantees that if the instrument is subject to damage or
fails to work normally due to the imperfect manufacture of our product, the
company will be responsible for the repair of the instrument (excluding
quick-wear parts and consumables) without charge within the warranty period of
12 months from the delivery date under the condition that the user complies with
the rules of transport, maintenance and operation. If the instrument is in need of
repair, please send it and its accessories back to our company, along with the
quality assurance card of our product.
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
2
Alert Symbols of Safe Operation
This symbol indicates important information of
operating the instrument safely.
Please read carefully and operate according to the
instructions following.
This symbol indicates a potentially hazard to the
electrical appliances through the operation, and
indicates that a qualified professional man is needed
to deal with such a hazard according to the
corresponding procedures. (This label is attached on
the power switch and trigger.)
This symbol indicates heating on the surface. Please
perform cautiously according to the instructions
following.
!
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
3
Points for Attention to Operation
1. The Spectro 96 Fluorospectrophotometer is suitably usedin a laboratory room to perform
analytical measurements. If the instrument needs to be used at a worksite, the working
environment of the worksite should accord with the demands of the laboratory environment
basically.
2. If the user needs to move the instrument to other working site for analytical measurements,
please use original packing materials on moving.
3. Be sure to turn on⁄off the power of the instrument according to the operating procedure.
When starting up the instrument, please turn on the power source of the xenon lamp first.
Then turn on the power source of the spectrophotometer main unit after the xenon lamp is
lit. When shutting down the instrument, please turn off the power source of the
spectrophotometer first and then the power source of the xenon lamp. Otherwise, the
instrument may not work normally. After the xenon lamp is lit, it is necessary to wait for 30
minutes over till the lamplight becomes stable. After the power source of the xenon lamp is
turned off, please wait for 60 seconds, and then turn on the lamp if the lamp needs to be
used again.
4. When the power source of the xenon lamp is turned on but the lamp fails to be lit and a
squeaking sound of high frequency or a cracking sound of sparking is produced
continuously, please turn off the power source of xenon lamp immediately and wait for
several seconds, then turn on the lamp again. Because the life of the xenon lamp is closely
related to the number of on-off of the lamp power source, the lamp should avoid being
turned on/off repeatedly under a high voltage and the unnecessary number of on/off of the
xenon lamp power source should be cut down as far as possible.
5. When the instrument is working, the surface temperature of the heat dissipation opening at
the top of the left back side of the instrument (xenon lamp position) becomes very high. Be
sure not to touch the surface of the heat dissipation opening with hands in order not to be
burnt. The air around the heat dissipation opening should be kept circulating.
6. After each startup of the instrument, please first confirm that the heat dissipation fan at the
side of the instrument runs normally in order to ensure normal work of the instrument. If
the user finds anything wrong with the heat dissipation fan, the instrument should be closed
down for an overhaul.
7. In order to protect the photomultiplier when PMT level is higher (>6), do not let high light
pass into the sample compartment. When a sample of unknown concentration is measured,
PMT level should be set from low grade to high grade (1-17) gradually.
8. After PMT level is adjusted, the original set for zero-point of fluorescence should be
checked and note that zero-point adjustment may be needed again.
9. When and only when a computer error is due to the operator¹s false operation or other
interferences, the power switch of the spectrophotometer main unit should be turned off
immediately. When the software cannot be operated, please use task manager to end the
“f96pc.exe” process, then restart the software and the instrument.
10. Within the monochromator the tightened screws must not be loosened. The optical devices
and the working environment of the instrument should be kept clean.
11. When opening the cover to inspect the instrument, be sure to turn off the power source.
Particularly after the upper cover of the instrument is removed, as there are electrical
appliance parts with high voltage located at the left back of the instrument base, be sure not
to contact these parts while the power source is still turned on.
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
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Contents
1Appearance and Performances of the Instrument..................................................................7
1.1 Appearance of the Complete Instrument and Main Components.............................7
1.1.1 The Complete Instrument .................................................................................7
Main Components................................................................................................................8
Main Components................................................................................................................9
1.2 Operating Panel of the Instrument.............................................................................11
1.3 Working Principles of the Instrument........................................................................12
Signal Processing and Control System...............................................................................12
Optical System...................................................................................................................13
1.4 Measurement Functions of the Instrument................................................................14
1.4.1 Working Modes of Measurement....................................................................14
1.4.2 Modes of Data Measurement...........................................................................14
1.4.3 Self-checking and Self-correcting ...................................................................14
1.5 Performance Index of the Instrument (Specifications).............................................15
2Operation of the Instrument...................................................................................................16
2.1 Operation of the Instrument Panel.............................................................................16
2.1.1 Display Window................................................................................................16
2.1.2 Operation of the Function Keys......................................................................16
2.2 Normally Working Condition of Startup...................................................................17
2.2.1 Startup of the Instrument................................................................................17
2.2.2 Confirmation of Heat Dissipation Fan Running............................................17
2.2.3 Initialization......................................................................................................17
Table 1-2 Fluorospectrophotometer Initialization List.........................................18
2.3 Measurement................................................................................................................19
2.3.1 Pouring the Sample Solution into Sample Cell..............................................19
2.3.2 QuantitativeAnalysis in Measurement Mode with a Single Instrument.....19
2.3.3 Shutdown Instrument......................................................................................21
3Installation of the Instrument.................................................................................................22
3.1 Installation Environment.............................................................................................22
3.1.1 Space Environment of Laboratory.................................................................22
3.1.2 Working Table ..................................................................................................22
3.1.3 Power Source ....................................................................................................22
3.1.4 Environmental Alternation..............................................................................22
3.2 Unpacking for Checking..............................................................................................23
3.2.1 Checking the Outer Packing ...........................................................................23
3.2.2 Unpacking for Counting..................................................................................23
3.3 Installation....................................................................................................................25
3.3.1 Cleaning ............................................................................................................25
3.3.2 Check of Power Source Matching...................................................................25
3.3.3 Connecting the Power Cord ............................................................................25
3.4 Adjustment of Instrument...........................................................................................26
3.4.1 Signal
Noise Ratio Test (Using Raman Spectral Peak of Water).................26
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
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3.4.2 Checking Wavelength of the Instrument........................................................28
4Maintenance of the Instrument...............................................................................................29
4.1
4.14.1
4.1 Routine Maintenance...................................................................................................29
4.2
4.24.2
4.2 Maintenance and Replacement of Light Source Lamp.............................................30
4.2.1 Maintenance of Light Source Lamp...............................................................30
4.2.2 Replacement of Light Source Lamp...............................................................30
4.3 Maintenance and Replacement of Filter....................................................................31
4.3.1 Maintenance of Filter.......................................................................................31
4.3.2 Replacement of Filter.......................................................................................31
5Environmental Requirements of Using Software and Installation......................................33
5.1 Basic Configuration of Computer Hardware and Software.....................................33
5.1.1 Hardware..........................................................................................................33
5.1.2 Software Requirements....................................................................................33
5.2 Installation of “F96 Software” ....................................................................................34
6Operation of Software..............................................................................................................35
6.1 Preparation before Use................................................................................................35
6.1.1 Connection of Spectro96Main Unit with a Computer......................................35
6.1.2 Procedure for On-line Operation (Startup)...................................................35
6.2 Three Interfaces of Working Mode.............................................................................37
6.2.1 The Interface of Wavelength Scan..................................................................37
6.2.2 The Interface of Time Scan.............................................................................37
6.2.3 The Interface of Quantitative Analysis...........................................................38
7Operation of Wavelength Scan Mode (Connected with a Computer).................................39
7.1 The Interface of Wavelength Scan Mode...................................................................39
7.1.1 The Functions of Shortcuts at the Bottom of the Screen..............................39
7.1.2 The Functions of Shortcut Icons.....................................................................40
7.1.3 Menu Bar at the Top of the Screen.................................................................40
7.2 Wavelength Scanning Operation ................................................................................41
7.2.1 Parameter Setting.............................................................................................41
7.2.2 Spectrum Processing........................................................................................43
7.2.3 Save Processing.................................................................................................45
7.2.4 Display Setting..................................................................................................46
8Operation of Time Scanning Mode (When Connected with a Computer)..........................47
8.1 Interface and Operation of Time Scanning Mode.....................................................47
8.1.1 Interface of Time Scanning Mode...................................................................47
8.1.2 Parameter Setting.............................................................................................47
8.1.3 Spectrum Processing........................................................................................48
8.1.4 Storage Processing............................................................................................48
8.1.5 Display Setting..................................................................................................48
9Operation of QuantitativeAnalysis Mode (When connected with a Computer)................49
9.1 Interface of Quantitative Analysis Mode ...................................................................49
9.1.1 The Functions of Short cuts on the Bottom of the Screen ............................50
9.1.2 The Functions of Shortcuts of Icon.................................................................50
9.1.3 Menu on the Top of the Screen........................................................................51
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
6
9.2 Operation of QuantitativeAnalysis............................................................................53
9.2.1 Setting of Fluorescence Wavelength for Measurement.................................53
9.2.2 PMT Setting......................................................................................................53
9.2.3 Zero-point Adjustment.....................................................................................53
9.2.4 Standard Sample Setting .................................................................................54
9.2.5 Measurement of Standard Samples................................................................54
9.2.6 Method of Undetermined Coefficients ...........................................................55
9.2.7 Measurement of Samples.................................................................................55
9.2.8 Set Sample Name..............................................................................................55
10 Appendix: Data Processing with Microsoft Excel 2000................................................56
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
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1Appearance and Performances of the Instrument
1.1
Appearance of the Complete Instrument and Main Components
1.1.1 The Complete Instrument
Operating Panel
Fig. 1-1 SPECTRO 96 Fluorospectrophotometer Main Unit
Heat Dissipation Opening at the Top of the Instrument
Heat Dissipation Fan
Power Line Lid of Sample
Compartment
Operating Panel
USB Interface
RS232 Communication Connector
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
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Fig. 1-2 The Left Side View of SPECTRO 96 Fluorospectrophotometer Main Unit
Sample Compartment (inside it a cell holder used to hold a sample cell), Power
Switch (used to turn on/off the power source of the instrument), Fuse Holder (used to
mount a fuse), Power Socket (used to interconnect a feed cable), Xenon Lamp Switch
(used to turn on/off the Xenon lamp).
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
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Main Components
1. Xenon Lamp
Fig. 1-3 Xenon Lamp and Lamp Holder
Line
Xenon Lamp (The
lightening part)
Line
Lens
INSTRUCTION MANUAL FOR SPECTRO 96 FLUOROSPECTROPHOTOMETER
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