Milwaukee MA871 User manual

MA871

Refractometer for SucroseRefractometer for Sucrose

Refractometer for Sucrose

MeasurementsMeasurements

Measurements

INSTRUCTION MANUAL

Milwaukee Refractometer

Test Equipment Depot - 800.517.8431 - 99 Washington Street Melrose, MA 02176

TestEquipmentDepot.com

Instruction Manual MA871 Refractometer for Sucrose Measurements

TABLE OF CONTENTS

FUNCTIONAL DESCRIPTION ............................................................................. 3

GENERAL DESCRIPTION .................................................................................... 5

SPECIFICATIONS .............................................................................................. 6

PRINCIPLE OF OPERATION ................................................................................ 7

MEASUREMENT GUIDELINES .............................................................................. 8

CALIBRATION PROCEDURE ............................................................................... 9

MEASUREMENT PROCEDURE ........................................................................... 10

MAKING A STANDARD % BRIX SOLUTION ........................................................ 11

CHANGING TEMPERATURE UNIT ..................................................................... 12

ERROR MESSAGES ........................................................................................... 13

BATTERY REPLACEMENT ................................................................................... 14

WARRANTY ..................................................................................................... 15

FUNCTIONAL DESCRIPTION

DISPLAYDISPLAY

DISPLAY

A. BATTERY STATUS ICON (BLINKS WHEN LOW BATTERY CONDITION DETECTED)

B. MEASUREMENT IN PROGRESS TAG

C. SETUP: FACTORY CALIBRATION TAG

D. CAL: CALIBRATION TAG

E. PRIMARY DISPLAY (DISPLAYS MEASUREMENT AND ERROR MESSAGES)

F. AUTOMATIC TEMPERATURE COMPENSATION

(BLINKS WHEN TEMPERATURE EXCEEDS 10-40 °C / 50-104 °F RANGE)

G. TEMPERATURE UNITS

H. SECONDARY DISPLAY (DISPLAYS TEMPERATURE MEASUREMENTS; WHEN BLINKING,

TEMPERATURE HAS EXCEEDED OPERATION RANGE: 0-80 °C / 32-176 °F)

Instruction Manual MA871 Refractometer for Sucrose Measurements

H. BATTERY COVER

I. BATTERY COMPARTMENT

BOTTOMBOTTOM

BOTTOM

A. LIQUID CRYSTAL DISPLAY (LCD)

B. READ KEY (USER MEASUREMENT)

C. ZERO KEY (USER CALIBRATION)

D. ON/OFF

E. PRIMARY DISPLAY

F. SECONDARY DISPLAY

G. STAINLESS STEEL SAMPLE WELL AND PRISM

FRONT PANELFRONT PANEL

FRONT PANEL

GENERAL DESCRIPTION

SIGNIFICANCE OF USE

Thank you for choosing Milwaukee. This instruction manual will provide you the necessary

information for correct use of the meter.

The MA871 is an optical instrument that employs the measurement of refractive index to

determine the % Brix of sugar in aqueous solutions. The method is both simple and quick.

Samples are measured after a simple user calibration with deionized or distilled water.

Within seconds the instrument measures the refractive index of the sample and converts it

to % Brix concentration units. The MA871 digital refractometer eliminates the uncertainity

associated with mechanical refractometers and is easily portable for measurements in the

field.

The measurement technique and temperature compensation employ methodology

recommended in the ICUMSA Methods Book (Internationally recognized body for Sugar

Analysis).

Temperature (in °C or °F) is displayed simultaneously with the measurement on the large

dual level display along with icons for Low Power and other helpful message codes.

Key features include:

• Dual-level LCD

• Automatic Temperature Compensation (ATC)

• Easy setup and storage

• Battery operation with Low Power indicator (BEPS)

• Automatically turns off after 3 minutes of non-use.

Remove the instrument from the packing materials and examine carefully to

ensure no damage has occurred during shipping. If any damage has

occurred, notify your Dealer.

Each MA871 instrument is supplied with:

• 9 V battery

• Instruction manual

Note: Save all packing material until you are sure that the instrument

functions correctly. A defective instrument must be returned in its

original packing.

Instruction Manual MA871 Refractometer for Sucrose Measurements

Range 0 to 85% Brix 0 to 80 °C (32 to 175 °F)

Resolution 0.1% Brix 0.1 °C (0.1 °F)

Accuracy ± 0.2% Brix ± 0.3 °C (± 0.5 °F)

Light Source Yellow LED

Measurement Time Approximately 1.5 seconds

Minimum Sample Volume 100 PL (cover prism totally)

Sample Cell SS ring and flint glass prism

Temperature Compensation Automatic between 10 and 40 °C (50 to 104 °F)

Case Material ABS

Enclosure Rating IP 65

Battery Type/Life 1 x 9 volt AA batteries / 5000 readings

Auto-Shut off After 3 minutes of non-use

Dimensions 19.2 x 10.2 x 6.7 cm (7.5 x 4 x 2.6")

Weight 420 g (14.8 oz.).

SPECIFICATIONS

PRINCIPLE OF OPERATION

The Brix determination is made by measuring the refractive index of a solution. Refractive

Index is an optical characteristic of a substance and the number of dissolved particles in

it. Refractive Index is defined as the ratio of the speed of light in empty space to the speed

of light in the substance. A result of this property is that light will “bend”, or change

direction, when it travels through a substance of different refractive index. This is called

refraction.

When passing from a material with a higher to lower refractive index, there is a critical

angle at which an incoming beam of light can no longer refract, but will instead be

reflected off the interface. The critical angle can be used to easily calculate the refractive

index according to the equation:

sin (Tcritical) = n2/ n1

Where n2is the refractive index of the lower-density medium; n1is the refractive index of

the higher-density medium.

In the MA871, light from an LED passes through a prism in contact with the sample. An

image sensor determines the critical angle at which the light is no longer refracted

through the sample. The MA871 automatically applies temperature compensation to the

measurement and converts the refractive index of the sample to sucrose concentration in

units of percent (by weight) Brix.

Instruction Manual MA871 Refractometer for Sucrose Measurements

MEASUREMENT GUIDELINES

• Handle instrument carefully. Do not drop.

• Do not immerse instrument under water.

• Do not spray water to any part of instrument except the “sample well” located over

the prism.

• The instrument is intended to measure sugar solutions. Do not expose instrument or

prism to solvents that will damage it. This includes most organic solvents and extremely

hot or cold solutions.

• Particulate matter in a sample may scratch the prism. Absorb sample with a soft

tissue and rinse sample well with deionized or distilled water between samples.

• Use plastic pipettes to transfer all solutions. Do not use metallic tools such as

needles, spoons or tweezers as these will scratch the prism.

CALIBRATION PROCEDURE

Calibration should be performed daily, before measurements are made, when the

battery has been replaced, or between a long series of measurements.

1. Press the ON/OFF key, then release. Two instrument test screens will be displayed

briefly; an “all segment” screen followed by the percentage of remaining battery life.

When LCD displays dashes, the instrument is ready.

2. Using plastic pipettes, fill the sample well with distilled or deionized water.

Note: If the ZERO sample is subject to intense light such as sunlight or another strong

source, cover the sample well with your hand or other shade during the

calibration.

3. Press the ZERO key. If no error messages appear, your unit is calibrated.

(For a description of ERROR MESSAGES see page 13).

Note: The 0.0 screen will remain until a sample is measured or the power is turned off.

1010

Instruction Manual MA871 Refractometer for Sucrose Measurements

MEASUREMENT PROCEDURE

Verify the instrument has been calibrated before taking measurements.

1. Wipe off prism surface located at the bottom of the sample well.

2. Using plastic pipettes, drip sample onto the prism surface. Fill the

well completely.

Note: If the temperature of the sample differs significantly from the

temperature of the instrument, wait approximately 1 minute to

allow thermal equilibration.

3. Press the READ key. Measurement is displayed in units of % BRIX.

Note: The ATC tag blinks and automatic temperature compensation is disabled if the

temperature exceeds the 10-40 °C / 50-104 °F range.

4. Remove sample from the sample well by absorbing with a soft tissue.

4. Gently absorb the ZERO water standard with a soft tissue. Use care not to scratch

the prism surface. Wipe off the surface completely. The instrument is ready for

sample measurement.

Note: If instrument is turned off the calibration will not be lost.

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