LI-COR LI-600 User manual

Using the

Ll-600 Porometer/Fluorometer

Instruction Manual

Using the LI-600

Porometer/Fluorometer

Instruction Manual

LI-COR Biosciences

4647 Superior Street

Lincoln, Nebraska 68504

Phone: +1-402-467-3576

Toll free: 800-447-3576 (U.S. and Canada)

[email protected]

LI-COR Distributor Network:

www.licor.com/envdistributors

Regional Offices

LI-COR Biosciences GmbH

Siemensstraße 25A

61352 Bad Homburg

Germany

Phone: +49 (0) 6172 17 17 771

[email protected]

LI-COR Biosciences UK Ltd.

St. John’s Innovation Centre

Cowley Road

Cambridge

CB4 0WS

United Kingdom

Phone: +44 (0) 1223 422102

[email protected]

Notice

The information in this document is subject to change without

notice.

LI-COR MAKES NO WARRANTY OF ANY KIND WITH

REGARD TO THIS MATERIAL, INCLUDING BUT NOT

LIMITED TO THE IMPLIED WARRANTIES OF

MERCHANTABILITY AND FITNESS FOR A PARTICULAR

PURPOSE. LI-COR shall not be held liable for errors con-

tained herein or for incidental or consequential damages in con-

nection with the furnishing, performance, or use of this

material.

This document contains proprietary information, which is pro-

tected by copyright. All rights are reserved. No part of this doc-

ument may be photocopied, reproduced, or translated to

another language without prior written consent of LI-COR,

Inc.

Windows® is a trademark of Microsoft, Inc. macOS® is a trade-

mark of Apple, Inc., registered in the US and other countries.

Drierite is a registered trademark of the W.A. Hammond Drier-

ite Company. Excelon Bev-a-line is a registered trademark of

Thermoplastic Processes, Inc. All other trademarks and

registered trademarks are property of their respective owners.

This product may be covered by one or more U.S.

pending or issued patents. For more information, visit

www.licor.com/patents.

Printing History

Publication Number: 984-19037

Created on Tuesday, April 19, 2022

iii

Notes on Safety

This LI-COR product has been designed to be safe when oper-

ated in the manner described in this manual. The safety of this

product cannot be assured if the product is used in any other

way than is specified in this manual. The product is intended

to be used by qualified personnel. Read this entire manual

before using the product.

Equipment markings:

The product is marked with this symbol

when it is necessary for you to refer to

the manual or accompanying doc-

uments in order to protect against injury

or damage to the product.

WARNING Warnings must be followed carefully to

avoid bodily injury.

CAUTION Cautions must be observed to avoid

damage to your equipment.

Manual markings:

Warning Warnings must be followed carefully to

avoid bodily injury.

Caution Cautions must be observed to avoid

damage to your equipment.

Note Notes contain important information and

useful tips on the operation of your

equipment.

CE Marking:

This product is a CE-marked product. For conformity inform-

ation, contact LI-COR Support at [email protected]. Out-

side of the U.S., contact your local sales office or distributor.

California Proposition 65 Warning

WARNING: This product contains chemicals known to the

State of California to cause cancer and birth defects or other

reproductive harm.

Federal Communications Commission

Radio Interference Statement

WARNING: This equipment generates, uses, and can radiate

radio frequency energy and if not installed in accordance with

the instruction manual, may cause interference to radio com-

munications. It has been tested and found to comply with the

limits for a Class A computing device pursuant to Subpart J of

Part 15 of FCC rules, which are designed to provide a reas-

onable protection against such interference when operated in a

commercial environment. Operation of this equipment in a res-

idential area is likely to cause interference in which case the

user, at his own expense, will be required to take whatever

measures may be required to correct the interference.

Waste Electronic and Electrical Equip-

ment (WEEE) Notice

This symbol indicates that the product is to be col-

lected separately from unsorted municipal waste. The

following applies to users in European countries: This

product is designated for separate collection at an

appropriate collection point. Do not dispose of as

household waste. For more information, contact your local dis-

tributor or the local authorities in charge of waste man-

agement.

Contents

Section 1. The LI-600

Components 1-2

Accessories kit 1-3

Support 1-4

Power and battery 1-4

Section 2. Using the LI-600 interface

Select Configuration 2-1

Measurement 2-2

Data labels and remarks 2-3

Matching 2-5

Scan Barcode 2-5

Instrument Settings 2-5

Info 2-6

Section 3. Taking Measurements

Measurement precautions and techniques 3-1

Precautions 3-1

Techniques 3-2

Taking a measurement 3-3

Taking a measurement in Auto Mode 3-3

Taking a measurement in Manual Mode 3-4

Using the barcode scanner 3-5

Viewing measurement data on-screen 3-6

Section 4. Using the computer software

Download the software and connect the LI-600 4-1

Connection Overview 4-1

Home screen 4-3

Toolbar 4-3

Drop-down menu 4-3

Buttons 4-4

Configuration Management 4-5

Data Management 4-7

Live View 4-9

Offline Mode 4-10

Barcode generator 4-11

Section 5. Configurations

Configuration settings 5-1

Creating custom configurations 5-4

General 5-4

Data Labels 5-5

Automode Setup 5-6

Remarks 5-7

Measurement Settings 5-7

Display Layout 5-9

Preview 5-10

Save Configuration 5-10

Section 6. Data files

Downloading data from the LI-600 6-1

Exporting data files from the computer software 6-2

Data file structure 6-2

Variables, units, and equations 6-3

Groups, labels and descriptions 6-3

Section 7. Troubleshooting

Instrument issues 7-1

Instrument will not power on 7-1

Instrument will not seal on a leaf 7-1

Instrument will not automatically log a measurement 7-2

The display layout is blank 7-2

vii

Software warning when connecting the LI-600 7-2

Software warning when updating the firmware 7-3

The new RH sensor serial number has not updated 7-4

The RH values and sample RH are high 7-4

Instrument warnings 7-5

Matching. Do not clamp on leaf. 7-5

Low memory 7-5

Low battery 7-5

Blower off 7-5

Pump 7-6

No GPS map pin 7-6

Data download errors 7-6

Section 8. Maintenance

Storing the LI-600 8-1

Cleaning the LI-600 8-1

Removing the instrument head shells 8-3

Removing and inserting a battery 8-5

Replacing the air intake filter 8-6

Calibrating the sensors 8-8

RH sensors 8-8

Flow sensors 8-16

Fluorescence detector 8-17

Changing the RH sensors serial number 8-17

Section 9. Theory of operation

Leaf porometer 9-1

Derivation of equations 9-2

Boundary layer conductance 9-5

Implementation of equations in the LI-600 9-5

Matching RH sensors 9-7

Flow sensors 9-7

Infrared temperature sensor 9-8

PAR sensor 9-8

Additional calculations for porometry 9-8

Stability criteria in auto mode 9-9

User calibration procedures 9-10

Fluorometer 9-13

Implementation on the LI-600 9-14

Calculating light intensity from LED calibration coef-

ficients 9-14

Dark-adapted vs light-adapted measurements 9-15

Steady-state fluorescence 9-16

Logged Variables 9-17

Porometry group 9-18

Fluorometry group 9-19

Leaf angle and GPS 9-20

Accelerometer/Magnetometer 9-20

GPS 9-21

Leaf angle software calculations 9-22

Clocks 9-24

References 9-25

Section 10. Specifications

General 10-1

Porometer 10-2

Fluorometer 10-3

GPS Receiver 10-3

Appendix A. FlashAnalysis App

Appendix B. Warranty

Appendix C. Index

viii

1-1

Section 1.

The LI-600

The LI-600 Porometer/Fluorometer is a lightweight hand-

held instrument that takes combined stomatal con-

ductance, and Pulse Amplitude Modulation (PAM)

chlorophyll afluorescence measurements of a leaf in

seconds. It delivers precise, high-throughput stress eval-

uations over the same leaf area and can quickly survey

plants under ambient conditions.

The LI-600 has a GPS receiver that records the latitude,

longitude, and altitude for each measurement, allowing

users to track, return to, and map locations. The LI-600

includes a magnetometer/accelerometer that measures the

pitch, roll and heading of a leaf. These measurements,

along with information from the GPSreceiver, enable the

LI-600 software to calculate the angle of incidence of a leaf

for each measurement.1

The LI-600 computer software is available for both Win-

dows®and macOS®. It is used to create and upload con-

figurations to the instrument, download data from the

instrument, record live data, and create barcodes. The soft-

ware is available for download at:

licor.com/600software

1GPS and the accelerometer/magnetometer are included in

models with a serial number higher than PFA-00553.

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