Diesse Chorus Trio User manual

SERVICE MANUAL

FOR CHORUS TRIO (REF. 81200) AND CHORUS TRIO RAS (REF. 81220)

REVISION 1 Issued in April 2021

Software version 7.xx

THIS MANUAL IS TO BE USED FOR CHORUS TRIO INSTRUMENTS STARTING

FROM S/N 4000 OR UPDATED WITH CHORUS EXPANDER UPGRADE KIT

(REF 83600)

FOR IN VITRO DIAGNOSTIC USE ONLY

This page is intentionally left blank

Rev. 1.0 (04-2021)

CHORUS TRIO EXPANDER | SERVICE MANUAL

CHORUS TRIO models:

The present manual refers to the following CHORUS TRIO models with the 7.xx family

of software installed:

Catalog number

Description

81200

CHORUS TRIO

81220

CHORUS TRIO RAS

Manual revisions list:

Manual revision

Description of changes

0 of 03/2021

First issue relative to Chorus TRIO with Expander

1 of 04/2021

Correction of paragraph 2.2 following the replacement of the

internal barcode reader

CHORUS TRIO EXPANDER | SERVICE MANUAL

Rev. 1.0 (04-2021)

Manufacturer

DIESSE DIAGNOSTICA SENESE SpA

Strada dei Laghi 39, 53035 Monteriggioni (SI) Italy

Tel. +39 0577 307109, Fax. + 39 0577 307106

www.diesse.it

TECHNICAL SUPPORT

Strada dei Laghi 39, 53035 Monteriggioni (SI) Italy

Tel. +39 0577 307109, Fax. + 39 0577 307106

Numero Verde: 800 606932

e-mail: technicalsupport@diesse.it

No page in this manual may be reproduced in any form or by any means, electronic,

mechanical or otherwise, for any use whatsoever without prior written permission

from DIESSE Diagnostica Senese S.p.A.

Rev. 1.0 (04-2021)

CHORUS TRIO EXPANDER | SERVICE MANUAL

Symbols:

Key of graphic symbols

Instrument meeting the requirements of the European Directive

98/79/EC on in vitro diagnostic medical devices (98/79/CE).

In vitro

diagnostic medical device

Manufacturing date

Serial number

Manufacturer

Key of electric and safety symbols

Protective conductor

WEEE: Waste electrical and electronic equipment –Separate

Collection is required pursuant to Legislative Decree No. 49 of

March 14. 2014 (Italy), on “implementation of Directive 2012/19/EC.”

Attention: please read this manual carefully and comply with the

safety symbols.

Caution: risk of electrical shock

Caution: laser beam

BIOHAZARD: potential risk of contamination handling infectious

substances

Crush hazard: moving parts can crush and cut; keep hands away

Risk of thermal shock due to the warming of the tray for samples

Risk of puncture to hands and fingers due to the presence of

needles for collection and dispensing

CHORUS TRIO EXPANDER | SERVICE MANUAL

Rev. 1.0 (04-2021)

LIMITATIONS AND WARNINGS

Version 7.00, revision 0 of this manual corresponds to the CHORUS TRIO model of the

instrument with the 7.xx family of software installed.

It was drafted and carefully reviewed and this version is closely related to the

instrument model

(data can be obtained from the instrument’s ID plate)

and the

version of the software that controls it

(data can be obtained through a procedure on

the instrument itself)

It must be read carefully before using the instrument, especially the parts relating to

safety.

ATTENTION

Do not use this manual except in its complete form.

If this manual is used in an incomplete form, DIESSE Diagnostica Senese S.p.A.

declines any responsibility for adverse results.

ATTENTION

Any instrument updating done with the customer’s authorization requires that the

user manual be updated in a corresponding manner.

DIESSE Diagnostica Senese SpA accepts no responsibility for damage caused

directly or indirectly by errors, defects, or incidents due to any use of this manual that

does not correspond with the version of the supplied instrument.

 
 
 
Rev. 1.0 (04-2021) 
CHORUS TRIO EXPANDER | SERVICE MANUAL 

 
 
Index: 
SOMMARIO 
1MOVING THE STRIPS................................................................................................................................................................... 9 
1 THE WORKSTATIONS............................................................................................................................................................. 9 
2 THE TRAY.......................................................................................................................................................................................10 
3 TRAY ROTATION .......................................................................................................................................................................10 
3.1 ABSOLUTE SENSOR .............................................................................................................................................................12 
3.2 STEP CHECK................................................................................................................................................................................12 
3.3 SYNCHRONIZATION.............................................................................................................................................................13 
4 STRIP HOUSING........................................................................................................................................................................14 
5 TRAY LOCK...................................................................................................................................................................................15 
6 CALIBRATIONS AND CONTROL PARAMETERS..................................................................................................15 
6.1 ALIGNMENT AND CENTERING OF THE PLATE..............................................................................................15 
2Strip recognition unit..................................................................................................................................................................17 
1 Strip presence sensor (SPS)...............................................................................................................................................17 
1.1 Check SPS sensor (Strip Presence Sensor).........................................................................................................17 
2 Internal barcode reader.......................................................................................................................................................18 
2.1 Internal barcode reader check and calibration ............................................................................................. 18 
3Transfer unit.....................................................................................................................................................................................20 
1 Dispensing hydraulic circuit............................................................................................................................................20 
2 The strip, needles and the well........................................................................................................................................21 
3 Dispenser.......................................................................................................................................................................................21 
4 X-axis movement....................................................................................................................................................................23 
5 The syringe unit........................................................................................................................................................................24 
6 The transfer function ............................................................................................................................................................25 
6.1 Transfer function parameters......................................................................................................................................25 
6.2 The double dispensing needle....................................................................................................................................25 
6.3 Priming of the dispensing and wash circuit................................................................................................... 26 
6.4 Withdrawal................................................................................................................................................................................. 26 
6.5 Drying of the tip...................................................................................................................................................................... 28 
6.6 Perforation of the cuvette membrane ................................................................................................................ 28 
6.7 Level detection........................................................................................................................................................................ 29 
6.8 Mixing............................................................................................................................................................................................. 30 
6.9 Simple transfer..........................................................................................................................................................................31 
7 CALIBRATIONS AND CONTROL PARAMETERS.................................................................................................33 
7.1 Circuit transfer..........................................................................................................................................................................33 
7.2 Dispensers ...................................................................................................................................................................................33 
7.3 X-axis movement .................................................................................................................................................................. 34 
7.4 Level sensor test......................................................................................................................................................................35 
7.5 Transfer test................................................................................................................................................................................35 
7.6 Strip perforation......................................................................................................................................................................37 
4Optical unit .......................................................................................................................................................................................38 
1 Light source................................................................................................................................................................................38 


CHORUS TRIO EXPANDER | SERVICE MANUAL 
Rev. 1.0 (04-2021) 
 
2 The optical device...................................................................................................................................................................38 
3 The optical channel ...............................................................................................................................................................39 
4 Positioning of filters.............................................................................................................................................................. 40 
5 Optical calibration .................................................................................................................................................................40 
5.1 Transmittance and absorbance................................................................................................................................40 
5.2 Alignment of the optical fibres................................................................................................................................... 42 
5.3 Calibration of the optical channels.........................................................................................................................44 
5.4 Calibration of the dark electronic offset..............................................................................................................44 
5.5 Calibration of light emission.........................................................................................................................................44 
5.6 The control ramp................................................................................................................................................................... 45 
5.7 Report of the virtual ramp..............................................................................................................................................46 
5.8 Control window......................................................................................................................................................................48 
6 Optical unit testing procedure ..................................................................................................................................... 49 
6.1 Setting of the optical filter offset...............................................................................................................................49 
6.2 Calibrating the offset (dark reading).....................................................................................................................49 
6.3 Calibrating the light............................................................................................................................................................49 
6.4 Check of absorbance at 650 nm with control solutions.................................................................. 50 
6.5 check of absorbance at 450 nm with control solutions...........................................................................51 
5WASHING unit................................................................................................................................................................................53 
1 Hydraulic wash circuit..........................................................................................................................................................53 
2 Tank probes ................................................................................................................................................................................54 
3 The washer ..................................................................................................................................................................................55 
4 The drying station...................................................................................................................................................................56 
5 Collection wells.........................................................................................................................................................................56 
6 Washing procedure ..............................................................................................................................................................58 
7 Washing circuit testing procedure.............................................................................................................................59 
7.1 Supplying of buffer from washer #1....................................................................................................................... 59 
7.2 Supplying of buffer from washer #2...................................................................................................................... 59 
7.3 Washing of the piping for washer #1..................................................................................................................... 59 
7.4 Washing of the piping for washer #2 ................................................................................................................... 59 
7.5 Aspiration at drying station #3...................................................................................................................................60 
8 CONTROL PARAMETERS.................................................................................................................................................. 60 
8.1 Filling level of washer #1 (step 21) .............................................................................................................................60 
8.2 Filling level of washer #2 (step 25)...........................................................................................................................60 
6The drain circuit.............................................................................................................................................................................62 
1 The main waste well..............................................................................................................................................................62 
1.1 Normal operation.................................................................................................................................................................. 63 
1.2 Obstructed drain...................................................................................................................................................................64 
1.3 Full waste tank........................................................................................................................................................................64 
1.4 Faulty drain pump................................................................................................................................................................ 65 
2 Waste circuit testing procedure...................................................................................................................................65 
2.1 Checking of waste level warning sensor (WS)............................................................................................... 65 
2.2 Checking of waste level error sensor (ES).......................................................................................................... 66 

 
 
 
Rev. 1.0 (04-2021) 
CHORUS TRIO EXPANDER | SERVICE MANUAL 

 
7Temperature control..................................................................................................................................................................67 
1 Temperature control of the instrument..................................................................................................................67 
2 Measuring chamber..............................................................................................................................................................67 
3 Heater ............................................................................................................................................................................................ 68 
3.1 Electrical properties of the heater...........................................................................................................................68 
4 Cycle temperature .................................................................................................................................................................69 
5 Stand-by temperature.........................................................................................................................................................69 
6 Procedure for testing the temperature control system...............................................................................69 
6.1 Checking of the instrument temperature sensor...................................................................................... 69 
6.2 Chamber temperature control.................................................................................................................................. 69 
6.3 Strip temperature correction ...................................................................................................................................... 69 
8Speaker-printer display.............................................................................................................................................................71 
1 Display .............................................................................................................................................................................................71 
2 The speaker..................................................................................................................................................................................71 
3 The printer ....................................................................................................................................................................................71 
3.1 Printer testing...........................................................................................................................................................................72 
9Electronic Parts..............................................................................................................................................................................73 
1 General Map...............................................................................................................................................................................73 
2 Carrier+IMX8 Board ...............................................................................................................................................................75 
2.1 Power supply and connections..................................................................................................................................75 
3 CPU 2010 Board and Driver 2010 Board...................................................................................................................76 
3.1 Power supply............................................................................................................................................................................ 77 
3.2 Description of the Test Points and CPU 2010 board jumpers...........................................................86 
3.3 DESCRIPTION OF THE TEST POINTS AND THE DRIVER 2010 BOARD JUMPERS ...........87 
3.4 Troubleshooting.....................................................................................................................................................................87 
4 Low Power...................................................................................................................................................................................87 
4.1 Description................................................................................................................................................................................. 87 
4.2 Power supply and connections.................................................................................................................................87 
4.3 Troubleshooting.....................................................................................................................................................................94 
5 Power Supply............................................................................................................................................................................ 94 
6 DESCRIPTION............................................................................................................................................................................95 
6.1 Troubleshooting...................................................................................................................................................................100 
7 Connectors board................................................................................................................................................................ 100 
7.1 Description...............................................................................................................................................................................100 
7.2 Troubleshooting................................................................................................................................................................... 103 
Service Procedures................................................................................................................................................................... 104 
1 Programming firmware CPU 2010........................................................................................................................... 104 
1.1 Service application.............................................................................................................................................................104 
1.2 Successful connection with CPU 2010 board.........................................................................................104 
1.3 Unsuccessful Connection with CPU 2010 board.................................................................................. 105 
1.4 Firmware upload on the CPU 2010 board.................................................................................................106 
1.5 Replacement of the Carrier+IMX8 board...................................................................................................108 
1.6 Methods saving...............................................................................................................................................................108 
1.7 Calibrations saving....................................................................................................................................................... 109 


CHORUS TRIO EXPANDER | SERVICE MANUAL 
Rev. 1.0 (04-2021) 
 
1.8 Software upgrade on the Carrier+IMX8 board...................................................................................... 109 
1.9 Methods and Calibrations upload on the Carrier+IMX8 board ...................................................111 
2 Programming BarCode reader Zebex Z 3080................................................................................................... 112 
2.1 Connect the reader to the instrument through the RS232 cable............................................112 
2.2 Disconnect the RS232 cable...................................................................................................................................112 
2.3 PROGRAMMING PARAMETERS..........................................................................................................................113 
3 Maintenance.............................................................................................................................................................................118 
3.1 Routine maintenance..................................................................................................................................................118 
3.2 Periodic maintenance.................................................................................................................................................118 
3.3 Wash well for the dispenser needles...............................................................................................................119 
3.4 Dispensers #1 and #2....................................................................................................................................................121 
3.5 X-axis guide..........................................................................................................................................................................121 
3.6 Washers 21 –25 –28......................................................................................................................................................122 
3.7 Optical unit..........................................................................................................................................................................123 
3.8 Washer wells......................................................................................................................................................................125 
3.9 Upper plate..........................................................................................................................................................................126 
3.10 Carousel .................................................................................................................................................................................126 
3.11 Peristaltic pumps ...........................................................................................................................................................126 
3.12 Diaphragm pump .........................................................................................................................................................127 
3.13 Hydraulic waste and syringe unit......................................................................................................................127 
3.14 Hydraulic unit....................................................................................................................................................................128 
3.15 Syringe Unit ........................................................................................................................................................................128 
3.16 Rotation..................................................................................................................................................................................129 
3.17 Tray synchronization device ..................................................................................................................................129 
3.18 Led box and filter handler .......................................................................................................................................129 
3.19 Tank probes ........................................................................................................................................................................129 
3.20 Tubing .................................................................................................................................................................................... 130 
 
 
 