SPEX NanoSNAP User manual

Operating Manual

Spex NanoSNAP™

Nano Spectrophotometer for Nucleic Acids

and Proteins Measurement

Operating Manual

1300-87076-1A Rev. 1

Contents

1.0 Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

1.1 Intended Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

1.2 General Instrument Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4

1.3 Chemical Waste Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

2.0 General Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.1 Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

2.2 Product Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

3.0 Getting Started . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3.1 Unpacking. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

3.2 Initial Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10

3.3 Main Screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10

3.4 Detection Arm Opening/Closing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

3.5 Adjusting the Pathlength Selector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

3.6 Basic Operation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12

4.0 Application: Nucleic Acid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14

4.1 Overview of Screen Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14

4.2 Protocol Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15

4.3 Calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

5.0 Application: Protein A280 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.1 Overview of Screen Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

5.2 Protocol Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18

5.3 Calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

6.0 Application: Protein Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.1 Overview of Screen Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

6.2 Protocol Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

6.3 Calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24

7.0 Application: OD 600 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7.1 Overview of Screen Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

7.2 Protocol Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

7.3 Calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27

8.0 Application: More Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

8.1 Factor Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

8.2 Std. Curve Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31

8.3 UV-Vis Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35

Operating Manual

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9.0 System Setting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

10.0 History Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39

10.1 Duplicating Report Setting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

10.2 Viewing a Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

11.0 User Folder Management . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

11.1 Creating a New User Folder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

11.2 Viewing a User Folder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

11.3 Editing a User Folder. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

11.4 Deleting a User Folder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40

11.5 Using a USB Flash Drive as a User Folder . . . . . . . . . . . . . . . . . . . . . . . . . . .41

12.0 Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

12.1 Cleaning the Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

12.2 Cleaning the Quartz Glass. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41

12.3 Annual Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

12.4 Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

13.0 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42

13.1 Error Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43

Appendix A: Technical Speciﬁcations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44

Appendix B: Ordering Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45

Operating Manual

1300-87076-1A Rev. 1

1.0 Safety Precautions

Before using the Spex NanoSNAP microvolume spectrophotometer for the ﬁrst time, please read this entire

Operating Manual carefully. To guarantee problem-free, safe operation of the Spex NanoSNAP, it is essential to

observe the following section.

1.1 Intended Use

This instrument is intended to be used by trained personnel to perform solution analysis. In this manual we

assume that the user have knowledge of basic laboratory procedures and spectroscopic analysis.

1.2 General Instrument Safety

PHYSICAL INJURY HAZARD. Using the instrument in a manner not speciﬁed by Spex may result in

personal injury or damage to the instrument.

1.2.1 Transportation and Storage

This instrument should be transported and stored in an environment with a temperature of -10 ºC to 60 ºC,

relative humidity 20% to 80%.

1.2.2 Installation and Operation

1. Indoor use only

2. Do not use the device in a potentially explosive environment or with potentially explosive chemicals or

combustible substances.

3. Maximum altitude 2000 m

4. Pollution degree 2

5. Ensure enough free space around unit for adequate ventilation

6. Avoid placing the device in direct sunlight.

7. Install the device in a location free of excessive dust.

8. Install the device in a room with a temperature of 15 ºC to 30 ºC, relative humidity 20% to 80%.

9. Do not position the device so that it is dicult to disconnect from the mains supply using the mains plug.

10. Choose a ﬂat, stable surface capable of bearing the weight of the device.

11. Do not allow water or any foreign objects to enter the various openings of the device.

Operating Manual

1300-87076-1A Rev. 1

1.2.3 Electrical Installation

THIS INSTRUMENT MUST BE GROUNDED

The appropriate power supply and cord combination should be connected to the equipment BEFORE

connection to the mains supply.

Before connection, please ensure that the electrical supply is suitable for the power requirements below:

CurrentCurrent VoltageVoltage

1.0 A 100 V to 230 V ~ 50/60 Hz

The equipment is provided with a power supply and a selection of four mains cords, consisting of:

- Type B (US) for 120 V a.c. installations

- BS1363 style, 3-pin (UK), Type F (EU) and Type I (Aus / China) for 230 V a.c. installations

Select the power cord appropriate for your electrical installation and discard the others.

Mains Cord Replacement

Should one of the power cords not be suitable for connecting to the power supply, replace the plug with a

suitable alternative.

THIS OPERATION SHOULD ONLY BE UNDERTAKEN BY A QUALIFIED ELECTRICIAN

NOTE: Refer to the equipment speciﬁcation to ensure that the plug and fusing are suitable for the voltage and

current stated. The cores in the mains cord are as follows:

230 V a.c.230 V a.c. 120 V a.c.120 V a.c.

BROWN LIVE/HOT BLACK

BLUE NEUTRAL WHITE

GREEN/YELLOW EARTH GREEN

Should the mains cord require replacement, a cable of of harmonized code H05VV-F with a cross section of

0.75 mm (18 AWG) must be used. The IEC C13 connector (for connecting to the equipment power supply) must

be suitable for the supply voltage and current of the equipment.

IF IN DOUBT CONSULT A QUALIFIED ELECTRICIAN

Operating Manual

1300-87076-1A Rev. 1

1.2.4 Cleaning, Decontaminating and Servicing the Instrument

Before using a cleaning or decontamination method other than those recommended by the manufacturer, verify

with the manufacturer that the proposed method will not damage the equipment.

Switch o and unplug the device before cleaning, servicing or replacing the fuses. Repairs should be carried out

by authorized service personnel only.

1.2.5 Instructions for Removal from Use, Transportation or Disposal

Do not dispose of this product as unsorted municipal waste. Follow local municipal waste ordinances for

proper disposal provisions to reduce the environmental impact of waste electrical and electronic equipment

(WEEE).

European Union Customers: Call your local Customer Service Oce for equipment pick up and recycling.

1.3 Chemical Waste Safety

1.3.1 Chemical Waste Hazard

HAZARDOUS WASTE. Refer to Safety Data Sheets and local regulations for handling and disposal.

Operating Manual

1300-87076-1A Rev. 1

2.0 General Description

The Spex NanoSNAP is a fast, microvolume spectrophotometer that provides accurate and intuitive operational

experience in only 3 seconds. It enables the measuring of samples from 190 to 1,000 nm, a broad range which

oers ﬂexibility for experiment. With the replaceable sample window, users don’t have to worry about residues.

2.1 Features

• Large LCD touch panel enhances visibility and ease of operation

• Robust and modern outlook design

• Simple and easy-to-use graphical interface

• Multiple built-in protocol functions

• Fast measuring time at 3 seconds

• Wide measuring range from 190 to 1,000 nm

• Quartz sample window which protects the optical analysis system

• A nano hydrophobic coating layer on the quartz window

• Replaceable sample window avoids contamination

• Thoughtfully designed assist light

• Auto Run measurement

• Automatically creates operating history

• Cushioning design of detecting arm

2.2 Product Overview

Figure 1. Front View

Table 1. Detailed description for top view.

Name Function

Front Panel 7-inch high resolution color LCD with capacitive touch panel. It displays the current

status of the system and allows the user to operate the instrument.

USB Port For data output via USB ﬂash drive.

Detection Arm Detection arm with cushioning design to reduce closing impact.

Front panel

USB port

Detection Arm

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1300-87076-1A Rev. 1

Figure 2. Front View with Opened Detection Arm

Table 2. Detailed description for top view with opened detection arm.

Name Function

Cover Window Quartz glass with nano hydrophobic coating layer. It protects optical ﬁbers and also

reduces contamination.

Sample Window Quartz glass with nano hydrophobic coating layer. It has an indicating sample adding

design. It protects optical ﬁbers and also reduces contamination.

Pathlength Selector Light path of Spex NanoSNAP can be selected manually by pathlength selector

according to the absorbance (concentration) range dierence.

Indicator Light Assisting LED light which makes up for the lack of ambient light, ensuring sample

adding quality.

LED indicator light

Sample window

Cover window

Pathlength selector

Operating Manual

1300-87076-1A Rev. 1

Figure 3. Rear View

Table 3. Detailed description for rear view.

Name Function

Power Cable Socket Power cable socket, connect device to the AC power supply

Power Switch Power on/o switch

Product Label Indicates the model name, serial number, power speciﬁcation, and other important

information.

3.0 Getting Started

3.1 Unpacking

Once you open the Spex NanoSNAP package, conﬁrm that all of the following items are included:

• Spex NanoSNAP spectrophotometer

• Quick-Start Guide

• Power Adapter

• Four power cords (US, UK, EU, Aus/China)

• Calibration Report

If any of the items are missing, damaged or if any incorrect items are included in the package, please contact

your local Spex distributor or sales representative immediately.

Power switch

Power cable socket Product label

Operating Manual

1300-87076-1A Rev. 1

3.2 Initial Operation

Place the device on a steady, ﬂat table. Connect the power cord to the power socket at the rear of the device.

Switch on the device using the power switch at the rear of the device. The LCD will show the boot screen, start

initiation process, and then the “Spex NanoSNAP” title will be displayed. Please DO NOT open the detection

arm until system diagnosis is complete. Tap on the “Spex NanoSNAP” title to log into the Main Screen and start

your operation. Tap on the Spex NanoSNAP title again on the Main Screen to log out.

Switch o the device when not in use.

Note: The adapter is foolproof, which requires more focus to plug in and out.

3.3 Main Screen

On the Main Screen there are some informational items indicating the status of the Spex NanoSNAP; it also

contains 8 main function icons. Please refer to the following for the detailed descriptions (Figure 4, Table 4).

Figure 4. Main Screen Overview

Table 4. Main function icons.

Main Menu Function Icons

Nucleic Acid

To create and edit nucleic acid protocols

More Assays

To create and edit customized protocols

Protein A280

To create and edit protein A280 protocols

System

System settings

Protein Assay

To create and edit protein standard curve

protocols

History

To access stored reports

OD 600

To create and edit OD 600 protocols

User

User folder management

Lab name

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