Sper scientific 300003 User manual

SUGAR/BRIX

REFRACTOMETER

300003

INSTRUCTION MANUAL

SPER

SCIENTIFIC

- 2-

TABLE OF CONTENTS

Introduction ............................................... 3

Panel Description ...................................... 3

Operating Procedures ............................... 4-5

Temperature Correction ............................ 5

Temperature Correction Table .................. 6

Precautions ............................................... 7

Specifications............................................ 7

Warranty ................................................... 8

- 3-

Introduction: Your portable refractometer is a

precision optical instrument designed to measure

the concentration of sugar in aqueous solutions.

It utilized the standardized Brix scale which is ac-

curate and easy to read. The unit’s lightweight,

ergonomic design makes it convenient for both

laboratory and field applications. It is excellent

for quality assurance, process control require-

ments, and scientific research.

The refractometer operates on the principle that

as the concentration or density of a solution in-

creases, its refractive index changes proportion-

ately. The refractive angle measured by your re-

fractometer registers on the scale. The larger the

concentration of sugar in solution, the higher the

reading on the scale.

Panel Description:

Cover Plate (1)

Light reflecting window (2)

Refracting prism (3)

Calibration screw (4)

Telescopic barrel (5)

Scale selector (6)

Eyepiece with focus ring (7)

- 4-

Operating Procedures

1. With the COVER PLATE (1) open, carefully clean

the PRISM (3) and REFLECTING WINDOW (2)

with a soft cloth. Avoid scratching the surfaces.

2. Aim the refractometer toward a light source and

select the 0-50% scale by rotating the SCALE SE-

LECTOR (6).

3. Rotate the EYEPIECE (7) to obtain the clearest

focus.

4. Adjustment of the null (reference point):

A. Open the COVER PLATE (1).

B. Apply a few drops of pure distilled water onto

the REFRACTING PRISM (3).

C. Close the COVER PLATE (1).

D. Turn the CALIBRATION SCREW (4) so that

the dark and light boundary line coincides ex-

actly with the 0% line reading on the scale in

the 0-50% range.

5. Carefully dry the prism platform and the LIGHT

REFLECTING WINDOW (2) with a soft cloth.

6. To test your sugar sample, rotate the SCALE

SELECTOR (6) to select the 0-50% range or the

50-80% range.

7. Place a few drops of the test solution on the prism

and close the COVER PLATE (1) so the solution

spreads evenly on the prism.

8. Aim the light reflecting windows toward the light

source and focus the eyepiece on the boundary

line of the light and dark hemispheres.

9. The boundary line indicates the concentration of

sugar in the test sample.

10. If the boundary line is too low or too high to be

read on the scale you selected, rotate the SCALE

SELECTOR (6) to the other scale.

0-50%

scale

-50

-45

-40

-35

-30

-25

-15

-10

-5

-0

85—

80—

75—

70—

65—

60—

55—

50—

50-80%

scale

- 5-

11. After use, clean the refracting and illuminating

prisms with a soft cloth to remove any surface

residue.

12. The temperature of the null reference liquid should

be at the same temperature as the sample solu-

tion. For variations in temperature during use, the

null point should be recalibrated once every 30

minutes.

Temperature Correction

Your refractometer is designed to operate with distilled

water and sample solutions at a standard temperature

of 20°C (68°F). To compensate for temperature con-

ditions other than 20°C, use the Temperature Correc-

tion Table on Page 6.

Add to the reading when the temperature is above

20°C. Example: Actual temperature is 30°C. The

boundary line reads a concentration of 15%. Refer to

the Temperature Correction Table and locate the 15%

concentration column. Follow this column down to the

row that corresponds to the temperature of 30°C. At

the 0.78 correction point, the actual concentration per-

cent is 15% + 0.78%, or 15.78%.

Subtract from the reading when the temperature is be-

low 20°C Example: Actual temperature is 16°C. The

boundary line reads a concentration of 40%. Refer to

the Temperature Correction Table and locate the 40%

concentration column. Follow this column down to the

row that corresponds to the temperature of 16°C. At

the 0.30 correction point, the actual concentration per-

cent is 40% - 0.30%, or 39.70%.

If the null liquid (distilled water) is the same tempera-

ture as the test sample and the null line of distilled wa-

ter reads 0%, it is not necessary to refer to the correc-

tion table.

- 6-

TEMPERATURE CORRECTION TABLE

Concentration(%)

0 5 10 15 20 25 30 35 40 45 50 55 60 65 70

0.50

0.46

0.42

0.37

0.33

0.27

0.22

0.17

0.12

0.06

0.54

0.46

0.45

0.40

0.35

0.29

0.24

0.18

0.13

0.06

0.58

0.53

0.48

0.42

0.37

0.31

0.25

0.19

0.13

0.06

0.61

0.55

0.50

0.44

0.39

0.33

0.26

0.20

0.14

0.07

0.64

0.58

0.52

0.46

0.40

0.34

0.27

0.21

0.14

0.07

0.66

0.60

0.54

0.48

0.41

0.34

0.28

0.21

0.14

0.07

0.68

0.62

0.56

0.49

0.42

0.35

0.28

0.21

0.14

0.07

0.70

0.64

0.57

0.50

0.43

0.36

0.29

0.22

0.15

0.08

0.72

0.65

0.58

0.51

0.44

0.37

0.30

0.22

0.15

0.08

0.73

0.66

0.59

0.52

0.45

0.37

0.30

0.23

0.15

0.08

0.74

0.67

0.60

0.53

0.45

0.38

0.30

0.23

0.15

0.08

0.75

0.68

0.61

0.54

0.46

0.39

0.31

0.23

0.16

0.08

0.76

0.69

0.61

0.54

0.46

0.39

0.31

0.23

0.16

0.08

0.78

0.70

0.63

0.55

0.47

0.40

0.32

0.24

0.16

0.08

0.79

0.71

0.63

0.55

0.48

0.40

0.32

0.24

0.16

0.08

20 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0

0.06

0.13

0.19

0.26

0.33

0.40

0.48

0.56

0.64

0.72

0.07

0.13

0.20

0.27

0.35

0.42

0.50

0.57

0.66

0.74

0.07

0.14

0.21

0.28

0.36

0.43

0.52

0.60

0.68

0.77

0.07

0.14

0.22

0.29

0.37

0.44

0.53

0.61

0.69

0.78

0.07

0.15

0.22

0.30

0.38

0.45

0.54

0.62

0.71

0.79

0.08

0.15

0.23

0.30

0.38

0.46

0.55

0.63

0.72

0.80

0.08

0.15

0.23

0.31

0.39

0.47

0.55

0.63

0.72

0.80

0.08

0.15

0.23

0.31

0.40

0.48

0.56

0.64

0.73

0.81

0.08

0.15

0.23

0.31

0.40

0.48

0.56

0.64

0.73

0.81

0.08

0.16

0.24

0.31

0.40

0.48

0.56

0.64

0.73

0.81

0.08

0.16

0.24

0.31

0.40

0.48

0.56

0.64

0.73

0.81

0.08

0.16

0.24

0.32

0.40

0.48

0.56

0.64

0.73

0.81

0.08

0.16

0.24

0.32

0.40

0.48

0.56

0.64

0.73

0.81

0.08

0.16

0.24

0.32

0.40

0.48

0.56

0.64

0.73

0.81

0.08

0.16

0.24

0.32

0.40

0.48

0.56

0.64

0.73

0.81

Temperature °C

Add to reading Subtract from reading

- 7-

Precautions

With reasonable care, your refractometer’s reliability,

precision and optical performance will not change.

•Never submerge the unit, and do not let liquid to

seep into the unit’s body.

•Clean the refractometer after each use with a soft

cotton cloth. Do not scratch surface of the prisms.

•Store in a dry, clean, and non-corrosive environ-

ment.

•Avoid strong shocks.

Measuring Range 0-80%

Resolution 1%

Accuracy ±1%

Size 7x 1 1/2”

Weight 19 oz

Accessories Screwdriver, Carrying

Case, Transfer Pipette,

Distilled Water, Instruction

Manual, Registration Card

Specifications

- 8-

WARRANTY

Sper Scientific warrants this product against defects

in materials and workmanship for a period of five (5)

years from the date of purchase, and agrees to repair

or replace any defective unit without charge. If your

model has since been discontinued, an equivalent

Sper Scientific product will be substituted if available.

This warranty does not cover damage resulting from

accident, misuse, or abuse of the product. In order to

obtain warranty service, ship the unit postage prepaid

to:

SPER

SCIENTIFIC LTD.

7720 E. Redfield Suite 7

Scottsdale, Arizona 85260

WWW.SPERSCIENTIFIC.COM

[email protected]

Please Note: The defective unit must be accompanied

by a description of the problem and your return

address. Please be sure to return your warranty

registration card within ten (10) days of purchase.

Table of contents

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