Installation & Operating Procedures
SPECTRONIC 2ar series
.Change in wavelength
It is importantto insertthe blank and resetthe
displayto 100%T or O.OAeverytime the
wavelengthis changed.
Tip: The basic stepsfor taking measurementsare
highlighted inbold text in the following
instructions.
10.
Wipe the cell with a tissueandinsert the cell into
the sample compartment. Align theguide marks
andclosethe lid.
II. Read the appropriate value (%T or A) from the
meter.12.
Removethe cell from thesamplecompartmentand
repeatsteps9 through II for anyremaining
samplesolutions.13.
When all measurementsarecompleted,turnoff the
spectrophotometerby turning the Power
SwitchlZero Control counterclockwise until it
clicks. TransmittanceandAbsorbance
SPECTRONIC~ 200 and 200+
Notes:
.Flashing display
A flashing display indicates that the reading is out of
range and the I OO%T/OA control must be adjusted.
This adjustment controls an optical occluder which
regulates the amount of light passing through the
sample.
In %Tmode:
A reading greater than 200%T will cause the display
to flash.
-If the flashing reading is -1999, turn the
100%T/OA control clockwise until the display
operates normally.
-If the flashing reading is +1999. turn the
lOO%T/OA control counterclockwise until the
display operates normally.
In absorbancemode:
A reading greater than 2A will cause the display to
flash.
-If the flashing reading is -1999, turn the
100%T/OA control counterclockwise until the
display operates normally.
-If the flashing reading is +1999. turn the
100%T/OA control clockwise until the display
operates normally.
1. Turn onthe insn-umentbyturning the Power Switch
(knob ontheleft sideof insn-ument)clockwise.
Allow the spectrophotometerto warm up for at least
15minutesto stabilize.
2. After the warmup period, setthe desired
wavelength with theWavelength Control Knob.
3. Setthe ffiter lever to theappropriate position for
the selectedwavelength(not required for
SPECTRONICCB>20D).4.
Adjust the displayto O%Twith theZero Control
(knob onthe front left sideof the insn-ument).Make
surethatthesamplecompartmentis emptyandthe
coveris closed.
5. Setthe display mooe to TRANSMI1TANCE or
ABSORBANCE bypressingtheMODE control key
until the appropriatelED is lit.
6. Fill a cleancell with water (or anotherblank
solution) andwipe the cell with atissueto remove
liquid droplets,dustandfingerprints.
7. Place the cell in the sample compartment and
aligntheguide mark onthe cell with theguide mark
atthe frontof the samplecompartment.Pressthe
cell firmly into the samplecompartmentandclose
thelid.
8. Adjust the display to lOO%T or O.OAwith the
Transmittance/AbsorbanceControl (knob onthe
right sideof insn-ument).
9. Removethe cell from the samplecompartmentand
emptythewater.
10. Rinsethe cell twice with smallvolumesof the
solutionto be measuredandfill it with the
solution.
It mayrequire severalcompleteturns ofthe
lOOo/fiTcontrolto return to the properrange.
