Olympus FLUOVIEW FV300 User manual

User’s Manual

FLUOVIEW

FV300

CONFOCAL LASER SCANNING

BIOLOGICAL MICROSCOPE

Ver 4.3a

This user’s manual is for the software to be run on Olympus FLUOVIEW FV300 Confocal Laser

Scanning Biological Microscope. To ensure safety, obtain optimum performance and familiarize yourself

fully with this product, we recommend that you study this manual thoroughly before operation.

This user’s manual is composed of two volumes including “SYSTEM OVERVIEW”, “OPERATION

INSTRUCTIONS”, “MAINTENANCE” and “TROUBLESHOOTING”. Together with this manual, please

also read the “User’s manual FLUOVIEW FV300” and the instruction manual of the microscope in order

to understand overall operation methods. To ensure the safety operation of laser system, we

recommend you to study the manual of each laser and the light source equipment besides this manual.

Retain this manual in an easily accessible place near a system for future reference.

X6338

Petition

I . SAFETY GUIDE (Another manual)

II . SPECIFICATIONS (Another manual)

III . SYSTEM OVERVIEW

IV . OPERATION INSTRUCTIONS

V . MAINTENANCE

VI . TROUBLESHOOTING

CONFIGURATION

Page

CAUTION

1. Reproduction, copying or duplication of a part or all of this software and manual is prohibited.

2. The information described in this manual may be subject to change without notice.

Registered Trademarks

Microsoft, Microsoft Windows, Excel for Windows are registered trademarks of Microsoft Corporation.

Other brand names and product names are trademarks or registered trademarks of their respective owners.

FLUOVIEW MANUAL CONFIGURATION

2Page

FLUOVIEW MANUAL CONFIGURATION

The FLUOVIEW system uses two manuals including this “User’s Manual” and the on-

screen manual built into the software (“Online Help”).

The User’s Manual is composed of the five following volumes and subject matter:

I . SAFETY GUIDE (Another manual)

Describes notes and cautions on using the FLUOVIEW system and on types of

warning labels.

1 SAFETY CAUTION ............................................................................... I. 1-1

2 WARNING LABELS ........................................................................................... I. 2-1

3 CAUTION FOR SYSTEM OPERATION............................................................. I. 3-1

4 USER’S SAFETY PROTECTION MEASURES ACCORDING TO IEC60825-

1:1993+A1:1997+A2:2001 “LASER PRODUCT RADIATION SAFETY STANDARD”

..........................................................................................................................I. 4-1

II . SPECIFICATIONS (Another manual)

Describes the specifications of the FLUOVIEW system.

1 SPECIFICATIONS..............................................................................................II. 1-1

III . SYSTEM OVERVIEW (This manual)

Describes the outline of the FLUOVIEW system.

1 SYSTEM OVERVIEW ........................................................................................ III. 1-1

IV . OPERATION INSTRUCTIONS (This manual)

Describes the operation procedures of the FLUOVIEW system, for example,

methods for image acquisition and various image processing.

1 Getting Started FLUOVIEW ...............................................................................IV. 1-1

2 APPLIED OPERATIONS.................................................................................... IV. 2-1

Appendix A List of Tools........................................................................................ IV. A-1

Appendix B List of Hot Keys.................................................................................. IV. B-1

Appendix C Glossary............................................................................................. IV. C-1

Appendix D Formatting of Magnetic Optical Disk.................................................. IV. D-1

FLUOVIEW MANUAL CONFIGURATION

Page

Appendix E Converting Analysis Data into a Chart Using EXCEL ........................IV. E-1

Appendix F User Registration................................................................................IV. F-1

Appendix G USER REGISTRATION OF FV300 ...................................................IV. G-1

Appendix H Change of Default Folder for [File I/O] Panel.....................................IV. H-1

Appendix I List of Functions in the [Active Overlays] Dialog Box ..........................IV. I-1

Appendix J Hand Switch and Microscope Frame Function Allocation ..................IV J-1

V . MAINTENANCE (This manual)

Describes maintenance of the FLUOVIEW system.

1 Software Setup ...................................................................................................V. 1-1

2 Maintenance of Major System Units ...................................................................V. 2-1

VI . TROUBLESHOOTING (This manual)

Describes countermeasures in case trouble occurs.

1 TROUBLESHOOTING GUIDE ...........................................................................VI. 1-1

For Online Help, please see “1-3 Online Help” in “OPERATION INSTRUCTIONS” of this

manual.

NOTATIONS IN THIS MANUAL

4Page

NOTATIONS IN THIS MANUAL

This manual complies with the following notations.

Notation of Caution, Notes and Tips

Notation Description

Caution to prevent injuries to the user or damage to the product

(including surrounding objects).

NOTE

Note for the user.

TIP

Hint or one-point advice for user reference.

Notation of panel, Command Buttons and Dialog Boxes

Notation Description

[Acquire] panel The name of a panel, dialog box, list box or check box is

enclosed inside square brackets ([ ]).

<OK> button

<Open File> button

The name of a button in a panel or dialog box is enclosed

inside angled brackets (< >).

Notation of Mouse Operations

Notation Description

clicking Action of pressing, then immediately releasing the mouse

button.

double-clicking Action of clicking the mouse button twice in quick succession.

dragging Action of moving the mouse while holding down the mouse

button, then releasing the mouse button at the desired

destination.

(Note) In this manual, clicking, double-clicking and dragging involves pressing the left

button of the mouse, unless otherwise specified.

NOTATIONS IN THIS MANUAL

Page

Notation of key operations

Notation Description

Enter The name of a key is enclosed inside .

Alt The positive sign (+) expresses the combination of more than

one key operation.

For example, F1

Alt refers to pressing the

F1 key while holding the Alt key down.

Direction keys Generic names given to the o,m,nand

pkeys.

Notation of system-specific terms

Notation Description

XY observation

(Other observations)

Refers to observation with XY scanning.

(The same principle also applies to other observations such

as XZ, Xt, XYZ, XYt and XYZt.)

Note that some of the panels and dialog boxes shown in this manual are not the precise

reproductions of the originals. Some windows are resized to facilitate the reading and

some grayed-out characters are printed in readable characters.

This volume describes the outline of the FLUOVIEW FV300

system.

Please read this volume so that you can understand the

system before use.

On This Volume

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