Olympus BHA-P User manual

OLYMPUS POLARIZING MICROSCOPE

MODELS

BHA

-P&B;H.-P

ATTACHMENT

This

instruction rnancral has been written for the use

the Olyn~pusPolarizing Microscope

Modcl

CHA-P

and Polarizirl!j Attach~nentModel

131-I-P.

recommended

r-ead

the

manual carefully in order to familiarize yourself fully

with

the use

Ihe microscope on the

polarizing attachment so

lhal

you

can

ob~an

the

best

performance

and

~ffectiveness.

IMPORTANT

Observe

the

following points carefully:

Operation

Always

handle

the microscope

with

the

care

deserves, and avoid abrupt motions.

Avoid exposureof

the

microscope

direct sunlight.

dust

and vibration.

Only

use

the

tension adjustment ringfor altering the tension

the coarse adjustment.

not twist the two coarse adjustment

knobs

the

opposite directions sirnultane-

ously, which

might

cause damage.

Ascertain that

the

voltage selector switch on the base plate is set

conformwit11

the

local mainsvoltage.

Disconnect

the

line cord frorn

the

power outlet for fuse replacement.

Maintenance

Lenses

must

always

kept

clean. Fine dust

lens

surfaces

should

blown or

wiped off by means

an air blower

clean

brush.

Carefi~llywipe off oil

finger-

prints deposited

the

lens

surfaces

with

yaclzs moistened

with

small amount

xylene, alcohol or ether.

not use organic solutions

wipe

the surfaces of various components.

Plastic

parts, especially,should

cleaned with

neutral detergent.

Never disassemble the rnicroscope for repair

The microscope should

stored

its container immediately aftel- use. li this

not

possible, it slioi~ld

covered with

vinyl dust cover.

is best

kcap

objectives and

eyepieces in

desiccator, containingcjcsiccants.

Disconnect the linecord

frorn

the

power

soirrce before

fuse

replacement.

CONTENTS

STANDARD

EQUIPMENT

. . .

. .

it.

NOMENCLATURE

. .

I. ASSEMBLY

. .

. . . . .

1V.

IDENTIFICATIONAND FUNCTION OF VARIOUS

COMPONENTS

. .

V. OPERATION

. .

ElectricSystem

lnterpupillary Distance

and

Diopter

Adjustments

Light Path Selection

Centering

the Condenser

. .

Centering

the

Stage

Centering

the

Objectives

Use of

Iris

Diaphragms

Focusing Adjustment

. .

OrthoscopicObservation

10.

Conoscopic Observation

. .

11.

Photomicrography

VI. OPTICAL

DATA

. .

VII. TROUBLESHOOTING.

. .

STANDARD

EQUIPMENT

Optional

Accessories:

Mechanical

stage

for

polarizing

use Model

AH-FMP

Berek

cornpensalor

Modsl

AH-CTP

Qhjectivc

(srrain-free)

P020X

Model

Microscope

stand

wrth

aux~lrary

lens

BHA-F

Rcvo

lving

nosepiece

RH-PRE

l~itermediate

polarizing

a~tachmenl

AH-PA

Quarter

wave

plate

AH-TPI47

BHA-751-P

BHA-651-P

BH-P-1

Sensrt~ve

tint

plate

(53OrnpCI)

AH-TP530

Polarlztng

b~necular

tube

(30~)

BH-PB

130

Polari~lnq

tr~nocular

tube

(30~1

BH-PTH30

Circular

rotatable

stage

BH-SRP

Swing-out

condenser

-POC

Tungsten

lamp

house

BH-LH

30-watt

tungsten

filament

bulbs

LS30

Objecrrves

(strain-free)

BH-P-2

PO4

POIOX

P040X

PO1

00X

Iollj

111

K5X

(with

cross

hatrs)

1-1

WFlOX

(with

cross

ha~rs}

Eyepieces

WF1

(with

1011

00mm

micrometer)

BIKSX

WFlOX

Photo

~yep~ece

K5X

Spare

fuses

F~lter

45C

Immersion

nil

V~nyt

dust

cover

II.

NOMENCLATURE

Base

Ill.

ASSEMBLY

Tfic

141ctl1re

i~clow11111~t~atCsthe

scq~leritidl

~prncodr~r

assernbly

T!ie

nt~nhrvc

~ndtcrlt~

the

assembly

cjrrlnr

vilrlous

colnponenls. Rel~iove

dust

caps

before mounting

Lurnpunents.

Take

cars

Ireep

all

glaqs

surfaces cleat),

and

avalcl

si.4

al~4t111g

tlie

srrr

faces.

Eyepiece

Insert

the

objective

10X

inlo

the

fixed

aperture

the

nose-

piece.

Objective

Qbservat~on

tu11e

Clamping

screw

Quarter

wave

plate

Sensitive

rint

lntermedrate Dolarizing

tube

plate

Attach

the

tube

the

stand

wrth

rhe

letters

"OLYMPUS"

Berek

facing

in front

the

micro-

compensator

scope

(or

align

the

red

dots).

(TI

Mechanical

stage

Nosepiece

k---

Rotatable

stage

M~croscops

stand

Lamp

house

Insert

the

two

pins

the

aux~liary

lens ~nto

the

bush.

rng5 on

the

microscoae

stand

unt~l

the

lens

clicks

into

posi-

tion.

w~th

the

convex

surface

(with

engraving

"UPS1DE")

tac~ng

upward.

Align~ng

red dots,

condens-

mount

and

condenser,

Insert

the

condenser

inro

the

mount

Prior

mounting

the

stage.

rack

down

the

condenser

mount

all

the

way.

rernwe

the

stage,

(*move

rhe

cbn-

denser

firs[.

Insert

the

lamp

house

and

pres-

sing

the clamping

screw

the

bottom

the

base,

rotate

lamp

house

until

the

locating

hole

and

clamplng

screw

are

al~sned.

Electr~c

connection

Pluq 111

11i(!

t:o~hnr?ct~ng

cord

th~

lari~p

hocrsc

~hc

r~ccptactr:

tho

back

the

bds~

Inscr

onc

plz~g

the

linc

cord

the

Illis

cord

socket

rhe

llasc

and

coiincct

the

orher

or lug

rhe

[lnwer

outlet.

;

)

IV. IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS

L~ght

path

selector

kr~ol)

Pull out the selector knob all

the way for photomicrography.

Analyicl- scaic

Tension adjustment ring

Arrow mark ~ndicatesIncrease

in coarse adjustment tension.

Voltmeter

SIlcIinq cont~~ollever

For cont~nuouslyvariable light

intensity.

L~icvol~~g~

selector

switch

Mer.lianical tube length

adjust-

Clarnping screw for

mechanical stage

ngraved letters "IN" for in-

sertion

Bertrand lens into

the light path;

"OUT"

for

removal of the Bertrand lens

from the light

path.

45'

click stop lever

Clamping screw for

stage

uated.

Filter mount

Polarizer scale

Reading to

5'.

Field iris diaphragm ring

Polarirer rotation ring

Rheostat trimmer screw

screwdriver until the

voltmeter

indicates IV,

with the slid~ng control lever positioned

closest to vou (low voltage).

Larnp house clamping screw

Nosepiece

clamping

Automatic

the cap

the fuse holder, and

replace the defective fuse with

replacement fuse.

(Disconnect BEFORE RE-

PLACEMENT.)

A. Match the linevoltage selector switch to local mains voltage (seepage

6).

Summary of Putting the Microscope in Operation

B. Switch on the light source.

Model BHA-P

Rotate the trimmer screw until the voltmeter indicates

(page

Place a specirnen slide on the mechanical stage.

Remove the Bertrand lens and analyzer from the ligh! path.

F. Coarse focc~swith the

10X

objective.

Make interpirpillary and diopter adjustments (page

10)

Center the condenser (page

10)

I. Center the stage (page 12).

Center objectives other than

10X

(page 12)

Swing in the desired objective.

Set the condenser, analyzer and Bertrand lens correctly according to your microscopic

purpose (pages 13 and 14).

Adjust illumination system.

N. Adjust light intensity.

Finefocus.

Adjust aperture irisdiaphragm and field iris diaphragm (page

12).

(

Adjustment of illumination system

For biological use ol the Moclel BHA-P, however, remove the analyzer, Bertrand lens

and

sensitive tint plates, and place the high/low magnification selector lever into the

"L"

posi-

tion lor

and

lox,

aricl the

"H"

positior?for

20X. 40X

and

100X

object~ves.

Microscopic

Orthoscopic

observation

Conoscopic

observation

Cut

off this page at dotted line and

put

~t on the wall near the microscope for

use

reminder of

microscopic procedure.

Objective

100X

20X

100X

Intermediate

polarizingat-

tachment

OUT

Conder~ser

top lens

OUT

Highllow magnifica-

tion selector lever

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