Olympus MIC-D User manual

OLYMPUS

Quick Start Guide

Learn

more about your MIC-D, down-load free software and explore

vast image libraries at the Olympus MIC-D website.

Focus

Control

Knob

...

---

-Rotation

Ann

Slide

MIC

·O

Oblique

Illumination

Mode

LED

illuminator

C~anrr£1

(Wires

illuminator

Head

Diffusion

FlIler-

Condenser

Lens---

Stage

Glass

Body

Housing

Focus

ing"""':

Lens-

Reflected"'-

Light

Port

Zoom

Handle-

Ellipt iCally . nd

Ci~u

lllrty

Pol.,.tzod

Li9 h

••

Abs

orpti

on Spo<:lta 01Human Visual

,--

O.B _ G_

Detailed software installation instructions may also be found at

the

MIC-D Website

Quick

Start

Guide

In the lab or in the field, the Olympus MI

C-D

por

table

digita

l microscope

captures still and moving images instantly. This Quick Start Guide will

int

roduce you

the MIC-D's i

nnovat

ive design and spectacular imaging

capabilities, so

u'll be up and taking p

ictur

es in no time.

MIC-D Pa

rts

Explained

MIC-D

Software

Introduction

Choos

ingYour Specimen

MIC-D

Basic

Controls

Illumination Technique

Image Capture Window

Still Imagesand Mov

ies

Time Lapse Movies

Sav

ingImages

Library Window

- ViewingCaptured Images

Image

Process

ingWindow

Line Drawingand Annotation

Image Manipulation and Adjustme

nts

Polarized Light Kit

2-3

4-5

B-9

10-11

12-13

15-1B

21-22

Get

Know

The

M C-D

Lamp

Head

Light Diffuser~

Stage

'\.

Optical Zoom

Control

'\.

Magnification

Indicator

Base

Tilting

Lamp

Arm

(

Focus

Control

Light Intensity

Control

....._ Lamp

/;I

The Lamp Head contains an extremely bright white

E.D. This device is brighter than an

ordinary bulb and uses less power. The Lamp Head rotates slightly to allow fine tuning to

the illumination angle. Make sure th

it is rotated all the way clockwise for Transmitt

Light work (Brightfield) and all

the

way

unte r clockwise for Re

ected

ght work.

....._

ght Diffuser

The

ght Diffuser can be pushed in and pulled out. Keep the Diffuser a

the way in

during Transmitted

ght Illumination, and a

the way out for Re

ected

ght

Ill

umination. Vary as ne

eded

for Oblique and Darkfield settings.

....._I ilting Lamp Arm

Adjustments to the angle of the L

amp

Arm are the key to the differ

t illumination

techniques. Use the live image to guide adjustments. The Arm should be set to the

vertical position for Transmitted

Lig

ht, to 11 o'clock for

lique

Ill

umination

and

about

10:30 f

Darkfield. The arm should be rotated all the way counter clockwise for the

Reflect

ght Setting.

......

Gliding

Stage

The Gliding Stage is

the

platform to place specimens or samples for observation. The MIC-

D's Stage glides 5mm in any horizontal direction,

and

can be ro

tat

ed 360 degrees. Use

the Gliding Stage to adjust the specimen position. Reme

mbe

r t

hat

up, down and rotational

movements are inverted.

-.Lo

oom Control

The MIC-D boasts an all glass optical

zoo

m l

s to enable continuous magnification

changes. The magnification range is an amazing 22x to 255x.

-I

'vlagnification Indicator

Use

the

Magnification Scale to read the zoom

pow

er, by aligning

the

scale markings on

the blue

zoom

handle to the

spot

below. Adjust the Magnification Slide Bar in

the

Image

apture

software window to match the setting on

the

MIC-D, in order to calibrate

the

scale marking in

the

live image window and captured image.

....._ Light

Intensity.

Control

ght intensity (and focus) should be initially set with the

zoo

minimum pow

prevent under exposure. The

C-D's exposure setti ng will then be auto

mat

ically

adjusted to maintain consistent brightness in

the

live image. For Reflected

ght

Illumin

ion turn

the

Lig

ht Int

sity Control to maximum.

....._

ocus Control

Rotate this knob to bring specimens and samples into clear focus. The MIC-D's lens can

focus on surfaces up to 5mm above the stage.

MIC-D

Software

Image

Capture

Window

Library

Window

Image

Processing

Window

MIC-D

Software

The MIC-D Software enables you to view, capture, process and

annotate

images in

three

simple windows.

Io-_Image

Capture

Window

Capture and save still and moving images viewed in

the

Live

Image Window with

the

simple click of a mouse button.

You

can

view all of your captured images in

the

tile bar below

the

live

window. The scale bar, light intensity and image format may also

be adjusted here.

Io-_

bra

Window

The Library Window

where

you can view your captured

images as thumbnails or in

full

size. Still images may be

displayed side by side for comparison purposes. Moving images

may also viewed here.

Io-_Image

P-

rocessing

Window

Annotation, color and brightness adjustments are performed in

this window. Digital zooming as well as white and black

balancing functions are available for selection.

Choose

Your

ecimen

Sam

~le

With

the

MIC-D

inv

erted

lens

design,

you

can

image

just about any sample,

regardless

its

shape or whether

light

can

pass

through

it-

thanks

to the

Reflected

Light

capacity.

Micros

cope

slide

preparations and thin, translucent specimens can

viewed

Brightfield

,Oblique or

Darkfield

Use

Oblique

Illumination

highl

ight

structural details and

Darkfield

for

low

contrast specimens. Oblique

Illumination

will

shade

rises

and depressions

your

specimen orsample.

Darkfield

can be

used

to emphasize

color

Use

Refle

cted

Light

for

anything

large

and opaque.

Don't

forget

that

for

field

work

you

can

use

the

MIC-D

anywhere

you

can take a

laptop computer.

smitt

Light

!Brightfield!

Oarkfield

Reflected

Light

Obliq

Illumin

ation

MIC-D

Basic

Controls

Let's

start

getting

familiar

with

the

basic

hardware

controls

Keep

eye on the

Live

Image

Window

you

manipulate the

MIC-D

controls

Make

sure

that the

Light

Diffuser

pushed

in,

the

Illumination

Arm

the

vertical

position

and the

lamp

head

rotated

fully

clockwise

Turn

the

MIC-D's

light

source on and

increase

the

light

intensity

turning the

control

clockwise,

about one

half

far

will

The

MIC-D

will

automatically

make

adjustments tothe

light

intensity

after

initially

set.

Turn

the

Optical

Zoom

Control

to a

minimum

setting.

The

Optical

Zoom

Control

enables the

user

physically

adjust the

position

of the

microscope's

lens

to obtain

magnification

powers

from

22x

255x!

Bring

the

specimen

into

focus

the

Live

Image

Window

rotating

the

Focus

Control

Knob.

Make

any

final

adjustments to the

light

intensity.

Notice

that the

Gliding

Stage

Moves

freely

any

direction.

Moving

the

Gliding

Stage

instead

ofthe specimen or

sample

will

allow

you

scroll

the

image

smoothly,

even

high

magnification

When

you

have

found

an interesting deta

il,

center it

the

Live

Image

Window

and

zoom

in.

Continue to

make

adjustments to the

focus

needed.

Illumination

Techni9

_ue

There are four different i

umination techniques that you can use to

view your specimen or sample

Transmitted

ght

Use

thistechnique to viewconventional microscope slidesor especially

thin specimens.

This

technique

also

known

as 'Brightfield'.

1. Rotate the Illumination

Arm

fully

clockwise to the vertical position.

.Rotate the

Lamp

Head

fully

counter clockwise.

Push

in the

Light

Diffuser

Obli

que and Darkfield Illumination

Use

either of these techniques on any transmitted light specimen or

sample to emphasize contrast.

Use

Oblique Illumination to highlight

structural detail by shadowing the 'hills' and 'valleys'.

Use

Darkfield

to create a black background and to emphasize specimen color.

1. Rotate the

Lamp

Head counter-clockwise.

Pull

out the

Light

Diffuser.Adjustments to the light

diff-

user position

may

be required.

Slowly

rotate the Illumination

Arm

counter-clockwise:

For

Oblique Illumination rotate the arm

until

the back-

ground just begins to darken. Three dimensional details

should displaya shadowing effect.

For

Darkfield Illumination rotate the

Illum

ination

Arm

further counter-clockwise. Stop when the background

turns black.

Best

results are achieved at

high

magnification.

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