Olympus CK2 User manual

MODELCK

INVERTED

MICROSCOPE

.------WARNING

-------,

This instruction manual

for

use

the Inverted Micro-

scope Model CK2. Before

putting

the microscope

into

operation,

recommend

you

read this manual carefully

order

familiarize yourself

fully

with

the

use

this

micrO$Cope so that

you

may

obtain

optimum

performance.

BEFORE

USE

Operait,",on!ii:===~

iiJ

microscope

is;

aprecision

innrument,

always handle

with

the

care

deserves

and

avoid

abrupt

motions

and

5hocb.

When

you

retnO\le

the

mICroscope

Irom

the

styrofOilm

CCI5f!,

hold

the

stage legs at Aand B

(Fill

For

mcwemefll

the

microscope,

hold

the

stage

leg

and

lhe

observation

lube

nol

hold

the

lamp

hous-

ing pillar. (Fig.

4The light source incorPOriltflS a 6 V

20W

halogen

bulb.

not use

any

olher bulb.

5Avoid expO$Ure

the

microscope to

dirK!

sunlight, high

temperature

and

humidity,

dUSI

and

vibration.

Before use, ascertain thaI

the

line voltage selector switch

the

base

plate

the

microscope Stand

SIlt

con-

formity

wIth

the

local

line

Yoltage.

Specifications

the

eleetrical

components

differ

from

others

than

the

equipment

with 12QV

supply circuit,

the

eq.,ipment

in compliance with the reQUirements

Underwriull

Labor"o,i~.

Also,

the

equipment

not

needed

$~ect

volt

•.

7Before fuse

replacement,

dIsconnect

the

pawer

cord

from

the

outlet.

The

fuse

boK

Iocilted

the

back

the

micrOSCOPe

stand.

IF'9

Use

ascre.vdnYllJ 10 remove this

boK

•

Always

Qfound

the

micrOSCOPe.

Fig 1

Fig 2

:s'

•

Maintenance and

sto~

lens

surfaces

must

ajways

kept clean F,ne dust on lens surfaces should

blOWll

off

means

Mild

blower Carefully Wipe

off

011

or flngerpnnts on the lens surfaces

with

gauze ffiOlstened WIth a

small

amount

alcohol and ether (3 7) m1lrture, or xylene

not

use organiC solutions

wipe the surfaces of vanous components Plastic parts. especlllily. should

be cleaned With aneutral detergent

@Be careful not to spill the culture solution, etc.

spilled. It should be Wiped

off

immediately.

When

objectives are not

screwed

Into the nosepiece apertures. seal the apertures With dust plugs, which

Will protect

the

lenses located In the

lower

light path from dust. culture solution, etc.

When

not

use, the microscope should be covered WIth the dust cover provided.

@AtenSion

adjustment

ring

provided neKt

the coarse

focus

adjustment

knobs

With

thiS deVIce

the

tenSion

the

coarse adjustment IS freely adjustable

lor

elther heavy

IlQht

movement.

depending

upon

operator

prelerence

(Fig 3)

Applying

the

tiP

large

screwdriver

at a

groove

the

penphery

the

tenSIOn

adjustment

nng

CD.

rotate the flng

the

d,rectlon

the

arrow

Increase

the

tensIOn.

feverse

the

flng

loosen,

However, do

not

loosen the lenslOfl adJustment nng

tOO

much.

because thiS

may

cause

the

line

locus

adjustment knobs

slip. Fig 3

CONTENTS

SPECIFICATIONS I

STANDARD

CONFIGURATIONS

NOMENCLATURE

OPERATION

4-1

Assembly

and

Operation

Each

Component 4

4-2

Phase Contrast Observation 7

PHOTOMICROGRAPHY

5-1

Photomicrographic System

and

Optional Accessories 8

TROUBLESHOOTING

GUIDE

SPECIFICATIONS

,,~

Des:rjptiOn

Focus

adlustment

vertical movemel'll of

the

nosepiece

(stage

is tilled)

mNtIS

Focusadjustment

the

OOltfw/fine

adjustment

knobs. provided With tension adju$lmel'll mechanism.

Foo.aing range lfrom

lhe

focal point

lhe

stage

surflcel:

6mm upward

arod

21TWTl

down

...

Quadruple,

with

provision for mountingasimple lilter analyzer.

160 mm

(XI

240mm

(Y). Substage: 70mm

IXI

180

(V).

Anilchilble Traversing area

120

(XI x78

(Vl;

coaxial

low

drive control

knobl

the

rnechiJ~ical

stage fIQht

left

'ide

the

plain

stage;

provided

with

2culture

vessel

holders

Light source 6 V 20 Whalogen

bulb

Light

intensity

control Continuously variable by resistor

Illumination F

iller

holder For 45 mm-dia. filters.

Built-in filter Frosted.

heat

absorbIng filter

Condenser Ultra Iong-work.ing distance condenser

NA.

0.3.

W.O.

mm; detaChable.

Optics

for

observation Afacal

optical

system.

Inclined

45-;

interpupillary distance adjustment

range

through

mm;

With

adi-

Binocular

lube

opter IIdlUstment helicoid

tl'le left

51_

Inclined

45-;

Interpupillafy distance adjustment

range

53 through nmm; WIth d,opter

Trinocular tube IIdjustment helicoid

both

sleeves..

Provided WIth aa:ommodiU'on

lor

3annuli, ioc:tuding one annulus for lOX

(oJ,1K)

U$Ild

Lig,t

annulus slider

lor

2OX)

OOJ8C11Ye

and

twO anouli

lor

and

4QX

objectives; detachable

Plan

4XPl

NA.

0.13; W.O. 15.5 mm

Dl0XPL

NAO.25;

w.O.

7.2

For

phase

COtllfast

LWD CD 20XPL

NAO,40;

W.O. 5.4 mm

LWD COPlan 4QXPL

NAO.60;

W.O,

1.9mm

Dbl8Ctiv@s

(correction collar)

D4X

NAO.lO;

W.O. 18.2 mm

For

bright field

Dl0X

NAO.25;

W.D.

7.2mm

LWD

CD20X

N.A.0.40;

W.O.

5.4 mm

Dimensions 200 mm

(W)

340

mm (D) X469 mm (HI

Evepoint height

Bil"lOCUlar

lube 342 mm (13.5 in.). TrinOOJlar tube

332mm

(13.3in.1

Weighl

About

k.g

(15.4Ib.1

STANDARD

CONFIGURATIONS

......

,CK2·BIP CK2-81C

CK2·TRP

CI(l·lRe

ComPQtlent

-\ ,

Microscope

stand

with

lil<ed

binocular

lube.

including

CK2Bl·F 0 0 0 0

CK2-U LWCD cooclenllef

M,croscope stand. includir'lQ

CK2TR·F

0 0 0 0

CK2·ULWCD condenser

Trinouclar

lUbe

BH·TR45·W 0 0 0 0

SliIQe eXlenS;Qf1

plates

CK2·SS

0 0 00

(2~1

000

"~,

(211U)

(2PC:f!

Attachable

med'lllnical stage

CK2-MVR

0 0 0 0

Halogen

lamp

socke! LS20HM 00000000

Halogen bulbs

6V2ONHAl

00000000

~cord

uycp

00000000

\)4X

0000

DIOX

0 0 0 0

PCSPlan 4XPL 0 0 0 0

Objectives

PC010XPL

0 0 0 0

LWDCD20XPL

0 0 0 0

lWOCD20X 0 0

LWQCDPlan4QXPL 0 0

Phase

slider"

CK2·SL 0000

Phaseanoular

ring for CK2·RS40 0 0

40X

objective

(Bltering te!elcoPe

CT~

0 0 0 0

eyeplec:*

lOX

P(1)

CWHK10X

00000000

photo

eyepiece 5X

NFK5XLD

0 0 0 0

Fitter

set·'

PM·FIL-7 0 0 0 0

Note 0indicates the compatible componems

for

each

model.

'With

annul,

lor

<IX

and 10l2OX

obJeCtives

•

"Incl.

45LBD2N

&43ND25-W45 filters

Ell

Optional Accessories

Attachable

mechanical stage

Db"ective

E ,

....

(For exclusive ose w,th CK2·TRP/-TACI

omicrograptoiC

sy1tem

Ma\Jn,latl()fl changer

O~ing

atachment

IMT2-MVR

ULWOCO

Plan

4QXPL

WHK1QX&WHKI5X

BH2-eA

BHl.QA

NOMENCLATURE

•

CK2-BIP/-BIC

Bulb

socket

Phase slider

Ultra

long-working

distance

condenser

lamp

housing pillar

Petri dish

holder

Accommodates a

35mm.{jia.

petri dish.

Stage

extension

plate

Objoctive

NO$epiece

Power cord receptacle

Incorporatl!s(I fuse.

Fine

adjustment

knob

Coarse

adjustment

knob

Attachable mechanical

...

Provided

with

2specimen

holders.

Specimen

holder

2types

specimen holder

are available: Terasaki holder

and slide

glass

holder.

Filter

holder

(for

45mm·dia.

filter)

Use

the

cobalt

filter

(45Cllor

brightfield,and

thegreen

filter

(IF550l for phase contrast

(both

filters provided).

Aperture

iris diaphragm

can be stOpped down for

easier focusing on an

un·

stained specimen,

opooed

for

phase contrast.

Eyepiece

Diopter adjustmant ring

Use

thiS

ring

for correction of

dioptric

difference between

both

eyes.

Binocular

tube

Bulb voltage

adjustmenl

knob

Use

the bulb

voltage

lower than rated prolongs

its

expected service life.

Main switch

Photo

tube

Eyepiece

Tube length ll<:Ijustment

rings

Trinocular

tube

IBH·TR45·W)

OPERATION

the halogen

bulb

burnl

out

dJring

operation,

w.it

until the

defective bulb

and

socket

Ir.

completely cooled before bulb

reo

plac:em.nt

prltYlnl •burn.

lmen

the

OOnlaa pins' Icompletely into thesocket

(Fig.

NeYel'

use

excessi

....

force 10

twist

bend

the

bulb,

but

IIftltly

push

the

pirn,

not

lea.... fingefl)rinu,

1WtC.

the

mirror

surfac.

, . II

Rnudged,

should

wiped

with

gauu

moinened

with

smtll

tmount

t1cohol

tnd~

... 13:11

mixture.

(Fig.

Looking at the

bulb

from its

froot

direction,

ascertain thilt

the

lower- half

the

iICl'"ew

head

the

mirror

surface

coY8l"ed

the

filament. (Fig.

the

filtment

devilles

horizonttlly

from

the

center

the

screw

head,

the

bulb

tilU

the

right

left.

Attach

the

bulb

again

perpendiCl.llerly.

(FiG-

the filament

devilles

vertically,

the

contact

pim

tre

not

in-

serted

completely.

Pu~

the

pins

completely.

(Fig.

P\J1I

001 the

contact

pins

the

halogen

bulb

lrem

the

polyethylene

....

certain

not

love

fingerprinu

smudge5

tta

bulb.

aSet

1in«.¥Cll

Je1-aor_i

Set

the

line

vollage

"ector

PNllch

located

the

base

plate

the

mjqO$COpe

n¥ld

the

loc:al

line voltage IF'll 4

shows

the

setting

the

_itch

l00V),

Inna

!1ll.

the

h,llOgl!!n

ulb

For Ihe U.S.:

Frames sold

the

U.S.

meellhe

standard

end

8fe

selat

115V. Therefor,

Ihis

selector

switch

not

included.

Fig 4a

•

Fig 5

Polyethylene

bag

Correct

Deviated

(homonlally)

(venically)

• - I Assembly and Operation

Each Component

Fig

AttIC

1118

the

bulb

socbt

Aligning

lhe

guide

pins

lhe

guide holes

<I>

the

lamp houSing,

slowly insert

the

bulb

socket

completely

without

tilling. (Fig

Connectlog

the

mechanical Slage

Screw

the

mecharucal stilge

the

right

left side

the

plainstage

with

clamping screws IprOl/ided

lhe

underside

lhe

stage,

Ti\tlten

wilh

coin. (Fig.

Connecting

the

stage tKtellSlOn Platll$

The

extension

plales can be

allached

lhe

plaIn

stage

means

the

clamping screws 2in

the

same manner as

lhe

mechanical

''''''.

Mounti

the

trinocul,,'J.'.".""' _

The

trillOClJlar

lube

can

mounted

by loosening

the

observation

tub!l clamping knob and insening

the

circular dOl/etail

under

lhe

tube

into the microscope stand;

then

tighten

the

knob

again.

QMoontmg

"'h'.~ob_~·

~.;n;;::::::::::::::::J

SCrew

each objective inlO

the

nosepiece 1Irom

1(Mt

power

hi\tl

power.

rotating

the

nosepiece in I

dockwlse

d.rection,

IFIlJ.

Please

note

that

the

front lens

the

obj&ctive in

thl

inverted

microscope faces upward, and

exposed

contamr~ion

more than

the

objective

an upright microscope. Therefore,

clean

the

front

lens occasionally, and

th.re

are

empty

open·

ings in

the

nosepiece, use

the

dust

plugs prO\lided

prevent

dust

debris

hom

IllIing

into

the

nosepiece.

Anaching

the

mechanical

st~

ext_ion

P.:,'

••

.....

•

Fig 8

oInserting

the

eyepieces

Fig 10

Fig

Insert

the

eyep.eces

into

the

observatiOn

tube

lnterpupilliry

distance

8dju~.~m~~~'~==:::::::::J

Hold

the

k,....rled sliding grips

the

fight

and

left eyep.ece

sleevll$ With

both

haods

and

push

the

tubes

together

pull them

apart

laterally whIle kicking through

the

evepieees

with

both

eyes.

until perfect

bll'lOCUlar

vision

obtaIned

you

kn()N your

Interpupillary distance setllrlQ. set

ill

the

scale

<Z)

between

the

eyepiece

sleeves.

IFlg 10)

Diopter adjustment

Binocular

tube

Look at

the

Image through

lhe

right eyepiece

with

your

right

eye

and focus

the

specimen WIth

the

locus

knobs. Next. look

ing at

the

image through

the

left

eyeplElOe

With

your

left eve.

rOtate

the

dio';lIer

adjustment

riog s

locus

the

spec;.mert

without

using

the

locus knobs.

(F'9_

10)

Trinocul.

tube

After

interpupIllary distance

adjustment

step

rotate

the

tube

length

adjustment

(1l'1Q

the

right eyepIeCe sleeve

match

your interpupillary distance setting which

you

obtained

lI'om

the

scale. Looklog

the

image

throu\tl

the

right eyepIece. locus

lhe

specimen by

ffi8a1"l$

the

focus knobs, Next. looking Ihrough

the

left eyepIece,

rotate

the

tube

length

adjuSllTll!f1t

ring 2

locus

the

specimen

these

adjustments,

partocahlY

can

maintained when oblectives

are

changed.

with

touch-up adjust·

...

,,,

11=."

111

Il!I

U5B

the

mechanical

Fig

•

Fig

lAs

seen

through eyepiece tube,

with eyepiece removed}

ATetasaki holdef Iis oplJOnally available to hold aTetasaki plate

65mm-dia.

petri dish. while

optional

slide glass hoidel' 2

can

used

hold

slide glass

a54mm-dia.

petri

dIsh on

the stage IFig.

121

96-wel1

micro-titre

plate

a24-we1l micro-titre

plate

can

directly

mounted

the

stage. [Fig

121

*The

mm-di

•.

petri dish holder @provided

used

prevent

aspecimen

veu&!

from falling through

the

opening

the

plein

stage. (Fig.

121

mAperture iris diaphragm

aperture

iris diaphragm buill in

the

illuminator

used

limit

lhe

angular aPerture of

objecllVll in brightfjeld.

and

controllable

oblain

optimum

obitctiVll

perforfT\itf"lOll,

depending

upon

focal

IengIh.

contrast

and

r8lOlution. The ope'llng

IN!

aperture

ins

diaphragm should

matched

the

N.A

the

objecti..e in u

•.

often

preferable,

however,

Stop

down

lhe

apertUre d

pl'lfagm

about 70% through 80% of

the

ootectlVe

IF'O

131

unsuined

specimen can be

nsily

observed

with

stopped-down

aperture

in bright filtld.

If)

Sening

the

correction collar

An Inverted microscope is conveniently arranged

observe

weeimem

contained

vessels of various

bonom

thicknesses. In

order

al;hieve

optimum

obiectiVll performance of

lhe

inverted

micrOSCoPe, the high N.A. objectives are provided with a

correction

collar

<D.

[Fig 14)

The

correction collar

effective

with

aveueI

bottom

from

0to

2mm

thickness.

the

thickness of

the

vessel

bottom

known.

Match

the

CO'rection collar

thickness

the

vessel

bollom

the

collar

scal.

provided

2) "

lhe

thickness of

the

bollom

unknown:

The

OPtimum POSition for

the

correction

collar can

obta,....:l

from

the

image resolution.

After

focus

adjustment,

satisfac·

tory

sharp image is not

obtained,

rotate

1M correction collar to

the

right

and

left

lnat

you

can

compare

the

imagll!i

both

sides. Reset

lhe

collar

tnt

bener

Image, then S1arting from

lhis position, further rOtate

the

collar

the

right

and

left until

both

images can

Obtained

for

comparison with each Other.

As you repeat Ihis procedure several times,

you

can find besl

position for the correction collar, Refocus after rotaling the correc·

tion collar.

lEI

Filters

...:===:..

Optimum

selection

filters enhances the

elleaive

performance

observati(lfl and

photomicrography,

Filter

Pu""""

1InterlerfnO! 19reenl

4S.IF550

Enh¥Fces Pl'lam

CX)l'ltrast

ifn9S.

2Neutral

d."ity

(gray) 43ND2S-W45 Reduces hght intensity

w,thout

ctFaog

ngspect

....

characteristics

the

light

3OUrce.

3Light balancing 4S.LBD-2N For color photomicrography With daylight film.

,Heat abtorbing 45·HAo Absorbs heat waves from Ihe light

KlUrCl

pro·

teet

tnt

specimen.

*This

f,lt.r

bu,lt

the

CK2. It

recommended to

add.

second heat absorbing

fllt.r

for prolonged

I~'

2Phase Contrast Observation I

Legend comes

atop.

Legend comes atop,

Fig 15

@-@

Fig 18

DPre aration

the

philSe slider

Put the li!tlt annuli for

ill

and 40X

objectives inlO the phase

slider.

The

annulus@

for

lOX

also

use<!

for 20X objectives. The

annuli for 4X and

lOX

{lOX Iobjectives are centered by means of

the

centering

lever@,

(Fig. 15)

Mount the light annulus

for 40X objective into the center hole

the

phase slider (as

the

ARROW indicates).

l:'ko~

~rti:i~O~O~flJjh~.~p~h~LiIT;'~;:============::::l

Insert

the

phase slider

into

the

slot

the condenser

such a

manner that its engraved surface with figures faces upward. and

the notched side toward the lamp housing pillar.

Rotate

the aper-

ture iris diaphragm lever

all

the way

the

left, and insen the

green filter provided

intO

the filter

mount

(j).

(Fig. 16)

Cantratl 0

tha

;tu

Place aspecimen on the

st~

and focus

it.

Remove one

the

eyepieces from

the

observation tube

and

insert

the

centering telescope CT·5

provided into

the

observation

tube. {Fig. 17)

Ascertain

that

objective magnification and annular ring are matched.

Rotate

the

top

lens assembly

the

centering telescope

bring

the bright ring (light annulus) and

the

dark ring (phase annulus)

into focus.

Operate

the

centering lever of

the

light annulus until

both

annuli

are

concentric and superimposed. (Fig.

181

aspecimen slide or

the

bottom

surface

Icufture

ve$Sill

not flat. either annulus

likely

slip off

the

center while

centering the other.

such a

caSIl.

repeat centering adjustment

again. This trouble, however, rarefy occurs with hi!tler magnifica·

tions, so

that

the 40X

philSe

annulus needs no centering adjust·

ment.

After centering adjustment with

the

lOX

objective, check

the

centration with

the

20X objective.

After centration

complete, remove

the

CT.s

and insert

the

eye·

piece back into

the

observation tube.

Now

ready for phase contrast observation.

This manual suits for next models

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