Olympus BX51 User manual

INSTRUCTIONS

BX51/BX52

SYSTEM MICROSCOPE

A X 9 8 5 5

This instruction manual is for the Olympus System Microscopes Models BX51 and BX52. To ensure

the safety, obtain optimum performance and to familiarize yourself fully with the use of this micro-

scope, we recommend that you study this manual thoroughly before operating the microscope.

Retain this instruction manual in an easily accessible place near the work desk for future reference.

BX51/52

CONTENTS

4-5

6-7

8-20

TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE

3-2 Focusing Block ...................................................................................................................................................................................... 11

1Voltage Indication; 2Using the Light Intensity Preset Switch; 3Using the Filters

21-23

24-25

27-29

Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble

the microscope yourself, please read section 7, “ASSEMBLY” (pages 27 to 29) carefully.

IMPORTANT — Be sure to read this section for safe use of the equipment. —

1 NOMENCLATURE

3 USING THE CONTROLS

3-1 Base ................................................................................................................................................................................................................. 8-10

3-3 Stage............................................................................................................................................................................................................ 12-14

3-4 Observation Tube ...................................................................................................................................................................... 15-17

3-5 Condenser .......................................................................................................................................................................................... 18-19

1Centering the Condenser; 2Compatibility of Objectives and Condensers

3-6 Immersion Objectives ................................................................................................................................................................. 20

1Using Immersion Objectives

3-7 Objectives with Correction Collar ............................................................................................................................. 20

4 TROUBLESHOOTING GUIDE

5 SPECIFICATIONS

6 OPTICAL CHARACTERISTICS

7 ASSEMBLY

PROPER SELECTION OF THE POWER SUPPLY CORD ................................................................. 30-31

1Replacing the Fine Adjustment Knob;

2Adjusting the Coarse Adjustment Knob Tension; 3Pre-focusing Lever

1Placing the Specimen; 2 Adjusting the X- and Y-Axis Knob Tension;

3Rotating the Stage; 4Adjusting the Stage Height

1Adjusting the Interpupillar Distance; 2 Adjusting the Diopter;

3Using the Eye Shades; 4 Using Eyepiece Micrometer Disks;

5Selecting the Light Path; 6Adjusting the Tilt

1-3

— See this section for the replacement of the light bulb. —

BX51/BX52

IMPORTANT

SAFETY PRECAUTIONS (Fig. 1)

Fig. 1

Designated bulb 12V100WHAL (PHILIPS 7724)

12V50WHAL-L (LIFE JC)

This microscope employs a UIS (Universal Infinity System) optical design, and should be used only with

UIS eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed for the BX

series are also usable. For details, please consult Olympus or the catalogues.) Less than optimum

performance may result if inappropriate accessories are used.

1. Install the microscope on a sturdy, level table or bench so as not to block

the air vents on the underside of the base.

Do not place the microscope on a flexible surface, as this could result in

blocking the air vents and cause overheating or a fire.

2. The surfaces of the lamp housing on the rear of the microscope will

become extremely hot during operation. When installing the microscope,

make sure to allow ample free space (10 cm or more) around and in

particular above the lamp housing.

3. When installing the microscope, route the power cord away from the

lamp housing. Should the power cord come in contact with the hot lamp

housing, the power cord could melt and cause electric shock.

4. To avoid potential shock hazards and burns when replacing the light

bulb, set the main switch @ to “ ” (OFF) then disconnect the power

cord from the wall outlet in advance. Whenever you replace the bulb

during use or right after use, allow the lamp socket ‚ and bulb to cool

before touching. (Fig. 1)

#The microscope also incorporate a fuse (this should be replaced by

the manufacturer or an authorized agent).

5. Always use the power cord provided by Olympus. If no power cord is

provided, please select the proper power cord by referring to the section

“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of

this instruction manual. If the proper power cord is not used, product

safety performance cannot be warranted.

6. Always ensure that the grounding terminal of the microscope and that

of the wall outlet are properly connected. If the equipment is not grounded,

Olympus can no longer warrant the electrical safety performance of the

equipment.

7. Never insert metallic objects into the air vents of the microscope frame

as this could result in electrical shock, personal injury and equipment

damage.

Symbol Explanation

Indicates that the surface becomes hot, and should not be touched with bare hands.

Before use, carefully read the instruction manual. Improper use could result in personal injury to

the user and/or damage to the equipment.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

Warning engraving

position Lamp housing

(Warning against high temperature)

Fig. 2

1Getting Ready

Safety Symbols

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment

in the safest possible manner.

Warnings

Warning engraving is placed at parts where special precaution is required when handling and using the microscope.

Always heed the warnings.

1. A microscope is a precision instrument. Handle it with care and avoid

subjecting it to sudden or severe impact.

2. Do not use the microscope where it is subjected to direct sunlight, high

temperature and humidity, dust or vibrations. (For the operating condi-

tions, refer to section 5, “SPECIFICATIONS”.)

3. When moving the microscope, carefully carry it with the grasping part on

the rear of the arm and the base as shown in Fig. 2 (Weight: approx. 16

kg).

#If a module such as a photographic device is installed, the micro-

scope system will be very heavy. Remove the intermediate attach-

ment before carrying.

#Damage to the microscope will occur if you grasp it by the stage,

coarse/fine adjustment knob or binocular section of the observa-

tion tube.

4. The BX51/52 series can be used with up to two intermediate attach-

ments (e.g. a U-CA magnification changer, U-EPA2 eyepoint adjuster, etc.).

For restrictions when using two intermediate attachments, make sure to

read the instruction manual provided with the respective intermediate

attachments.

BX51/BX52

2Maintenance and Storage

1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly

moistened with a mixture of ether (70%) and alcohol (30%).

Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keep

these chemicals away from open flames or potential sources of electrical sparks –– for example, electrical equip-

ment that is being switched on or off. Also remember to always use these chemicals only in a well-ventilated

room.

2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean

them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.

3. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance.

4. When not using the microscope, keep it covered with a supplied dust cover.

3Caution

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the

equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

: Indicates that failure to follow the instructions in the warning could result in bodily harm to the

user and/or damage to equipment (including objects in the vicinity of the equipment).

# : Indicates that failure to follow the instructions could result in damage to equipment.

} : Indicates commentary (for ease of operation and maintenance).

NOMENCLATURE

Interpupillary distance

adjustment scale (Page 15)

Diopter adjustment ring

(Page 15)

Slide holder (Page 12)

Aperture iris diaphragm ring

(Page 19)

X-axis knob (Page 13)

Y-axis knob (Page 13)

Pre-focusing Lever (Page 11)

Filter mount (Page 9)

Field iris diaphragm ring

(Page 18)

Allen screwdriver

Condenser height

adjustment knob (Page 18)

Voltage indicator LED

Main switch (Page 1)

Light intensity preset

switch (Page 8)

Light intensity preset

adjustment screw

(Page 8)

Fine adjustment knob (Page 11)

Coarse adjustment knob (Page 11)

Coarse adjustment tension adjustment ring (Page 11)

Brightness adjustment knob (Page 8)

(Lamp voltage adjustment knob)

(accommodation position)

(Detachable)

} If you have not yet assembled the microscope, read section 7, “ASSEMBLY” (pages 27 to 29).

Transmitted light specification models BX51TF or BX52TF microscope frame.

Filter knobs (Page 9)

Optional filter knob (Page 9)

Light path selector knob (Page 16)

ND6, ND25 and LBD from the front to the rear

BX51/BX52

Transmitted/reflected ight

switch

Transmitted light/reflected light specification models BX51TRF and BX52TRF microscope frames

NOTE

* For the reflected illuminators and reflected lamp housings, please also refer to their instruction manuals.

The controls are identical to those of the transmitted light specification models except for the transmitted/

reflected light switch and the reflected light system (vertical illuminator, reflected lamp housing).

Reflected light illuminator*

· Universal illuminator

(BX-URA2)

· Fluorescence illuminator

(BX-RFA)

Reflected lamp housing*

· 100 W mercury burner housing

(U-LH100HG)

· 100 W mercury apo-burner housing

(U-LH100HGAPO)

· 75 W xenon lamp housing

(U-LH75XEAPO)

· 100 W halogen lamp housing

(U-LH100-3)

· System lamp housing

(U-ULH, halogen bulb specification)

(for exclusive use with

the halogen light source)

(Controls Used) (Page)

(P. 8)

Start observation.

(P. 16)

(P. 12)

(P. 13)

(P. 11)

(P. 15)

(P. 18)

(P. 19)

(P. 18)

(P. 9)

TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE

Set the main switch to “ I ” (ON) and adjust the

brightness. @Main switch

²Brightness adjustment knob

Set the transmitted light

(BX51TRF/BX52TRF only)

³Transmitted/reflected light

switch

Engage only the LBD filter. |LBD filter knob

Select the light path (trinocular tube). ƒLight path selector knob

Place the specimen on the stage. …Slide holder

†X-/Y-axis knobs

Engage the 10X objective in the light path. ‡Revolving nosepiece

Bring the specimen in focus. ŠCoarse/fine adjustment knobs

Adjust the interpupillary distance.

Adjust the diopter.

Adjust the light axis.

‰Binocular tube

‹Diopter adjustment ring

ŒCondenser height adjustment

knob

™Condenser centering knob

Adjust the aperture iris and field iris diaphragms. šAperture iris diaphragm ring

›Field iris diaphragm ring

Engage the objective to be used in the light path

and bring the specimen in focus. ‡Revolving nosepiece

Adjust the brightness. ²Brightness adjustment knob

Engage the required filters. œFilters

(P. 5)

(P. 9)

(P. 8)

BX51/BX52

²|

…

†

‡

‰

‹

™

} Copy the observation procedure pages on separate sheets and post it near your microscope.

›

USING THE CONTROLS

3-1 Base

Fig. 3

Fig. 4

1Voltage Indication (Fig. 3)

1. Turn the brightness adjustment knob @ clockwise to increase the volt-

age and make illumination brighter.

2. The numerals to the right of the lamp voltage indicator LEDs ² indicate

the voltage.

2Using the Light Intensity Preset Switch (Fig. 4)

}The light intensity preset switch @ makes it possible to limit the light

intensity to a preselected level regardless of the position of the bright-

ness adjustment knob.

The light intensity preset has been preset to optimum brightness for

photography when the built-in LBD filter is engaged (approx. 9 V with

marking).

1. Press the light intensity preset switch @ to the ON position. (The face of

the switch lights when it is ON.)

2. Using a small flat-blade screwdriver, turn the preset adjustment screw ²

to obtain the required light intensity. Turning the screw clockwise increases

brightness.

3. When the light intensity preset switch is set to OFF, the brightness returns

to the level set by the brightness adjustment knob.

#While the light intensity preset switch is ON, turning the light bright-

ness adjustment knob does not affect brightness.

3Using the Filters (Figs. 5 - 10)

}You can place a filter in the light path with one of the following methods.

· Push in the knob of the desired built-in filter to engage it in the light path.

(Page 9)

· Place a filter on the filter mount on the base and engage in the light path.

(Page 9)

· Insert a filter in the U-FC filter cassette, attach it on the filter mount and

engage it in the light path by sliding the filter lever. (Page 9)

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