Okolab LEO User manual

LEO

Handheld Analyzer for IVF Applications

Manual

IST1446_REV05

SV 3.1.64.0

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Index

1SYMBOL DESCRIPTION ..........................................................................................................................................................1

1.1 SYMBOLS USED IN THIS MANUAL AND ON THE PRODUCT ......................................................................................................................1

1.2 SYMBOLS ON THE PRODUCT LABEL ...........................................................................................................................................................1

2SAFETY NOTES..........................................................................................................................................................................2

3REGULATORY COMPLIANCE ................................................................................................................................................3

3.1 REGULATORY STATEMENT.........................................................................................................................................................................3

3.2 WASTE ELECTRICAL AND ELECTRONIC EQUIPMENT DIRECTIVE (WEEE DIRECTIVE)..................................................................3

4PREFACE .....................................................................................................................................................................................4

4.1 SENSOR MODULES.......................................................................................................................................................................................5

5SUPPLIED EQUIPMENT..........................................................................................................................................................6

5.1 POWER SUPPLY............................................................................................................................................................................................7

5.2 SODA LIME KIT ............................................................................................................................................................................................8

5.3 MOISTURE TRAP..........................................................................................................................................................................................8

5.4 OTG-CABLE..................................................................................................................................................................................................9

5.5 FITTING TUBES ............................................................................................................................................................................................9

5.6 CALIBRATION KIT........................................................................................................................................................................................9

5.7 TUBE-A.......................................................................................................................................................................................................10

5.8 TUBE-B.......................................................................................................................................................................................................10

5.9 ADDITIONAL TUBES..................................................................................................................................................................................11

5.10 CO2-O2-MODULE (EMBEDDED IN LEO) .........................................................................................................................................11

5.11 PUMP (EMBEDDED IN LEO) ...................................................................................................................................................................11

6OPTIONAL PARTS................................................................................................................................................................. 13

6.1 T1-MODULE...........................................................................................................................................................................................13

6.2 T2-MODULE...........................................................................................................................................................................................13

7LEO CONNECTION PORTS .................................................................................................................................................. 14

8PRELIMINARY OPERATIONS............................................................................................................................................. 15

8.1 CHARGE THE BATTERY.............................................................................................................................................................................15

8.2 DATE &TIME SETTING ............................................................................................................................................................................16

8.3 MEMORY CHECK .......................................................................................................................................................................................16

9NAVIGATION MENU ............................................................................................................................................................. 17

9.1 HOMEPAGE.................................................................................................................................................................................................17

9.2 ALARM ICONS AND MEANINGS................................................................................................................................................................19

9.3 SETTINGS....................................................................................................................................................................................................19

9.3.1 Device...............................................................................................................................................................................................20

9.3.2 Calibration......................................................................................................................................................................................21

9.3.3 Pump.................................................................................................................................................................................................22

9.3.3.1 Pump Leak Test ................................................................................................................................................................................................ 22

9.3.4 Alarm ................................................................................................................................................................................................25

9.3.5 System..............................................................................................................................................................................................26

9.3.5.1 Date & Time........................................................................................................................................................................................................ 27

9.3.5.2 Power Saving..................................................................................................................................................................................................... 28

9.3.5.3 Memory ................................................................................................................................................................................................................29

9.3.5.4 Info .........................................................................................................................................................................................................................29

9.3.6 Display .............................................................................................................................................................................................30

9.3.6.1 Brightness ........................................................................................................................................................................................................... 31

9.3.6.2 Theme ...................................................................................................................................................................................................................31

9.3.6.3 Touchscreen .......................................................................................................................................................................................................32

9.3.7 Units..................................................................................................................................................................................................32

9.3.8 Factory Reset.................................................................................................................................................................................33

10 CO2-O2 MEASUREMENT PROCESS.............................................................................................................................. 34

10.1 CO2-O2 MEASUREMENT PROCESS:PRELIMINARY OPERATIONS....................................................................................................35

10.1.1 Diffusion Mode –Preliminary Operations .......................................................................................................................35

10.1.2 Aspiration Mode –Preliminary Operations ....................................................................................................................37

10.1.3 CO2-O2 Single Point Measurement.....................................................................................................................................39

10.1.4 CO2-O2 Logging ...........................................................................................................................................................................40

10.1.5 Wet Mode........................................................................................................................................................................................42

11 TEMPERATURE MEASUREMENT PROCESS.............................................................................................................. 43

11.1 TMODULES –PRELIMINARY OPERATIONS..........................................................................................................................................43

11.2 TEMPERATURE SINGLE POINT MEASUREMENT ..................................................................................................................................44

11.3 TEMPERATURE LOGGING.........................................................................................................................................................................44

12 READING DATA.................................................................................................................................................................. 47

12.1 VIEWING DATA..........................................................................................................................................................................................47

12.2 DOWNLOADING DATA..............................................................................................................................................................................49

12.3 DELETING DATA .......................................................................................................................................................................................54

13 CO2-O2-MODULE CALIBRATION ................................................................................................................................. 57

13.1 VIEW CURRENT.........................................................................................................................................................................................58

13.2 SPAN CALIBRATION..................................................................................................................................................................................58

13.2.1 CO2/O2 User Calibration using Span Gas Cylinder: Preliminary Operations ..................................................59

13.2.1.1 Span Gas Cylinder with Pressure Regulator......................................................................................................................................... 59

13.2.1.2 Span Gas Cylinder with Flow Regulator................................................................................................................................................. 60

13.2.2 CO2/O2 User Calibration with Certified Gas at atmospheric pressure: Preliminary Operations ...........61

13.2.3 Span Setting ...................................................................................................................................................................................62

13.2.4 Start...................................................................................................................................................................................................64

13.2.4.1 Auto Gas calibration.......................................................................................................................................................................................64

13.2.4.2 Manual Gas Calibration ................................................................................................................................................................................65

13.3 FACTORY ....................................................................................................................................................................................................66

13.4 CO2 ZERO RESET .....................................................................................................................................................................................67

13.4.1 CO2 Zero Reset with Air...........................................................................................................................................................68

13.4.2 CO2 Zero Reset with N2 ...........................................................................................................................................................70

13.5 OFFSET .......................................................................................................................................................................................................74

14 T1 AND T2 MODULES CALIBRATION......................................................................................................................... 77

14.1 VIEW CALIBRATION..................................................................................................................................................................................77

14.2 START MULTI-POINT CALIBRATION .....................................................................................................................................................78

14.3 FACTORY CALIBRATION...........................................................................................................................................................................79

15 TOUCHSCREEN CALIBRATION ..................................................................................................................................... 81

16 CLEANING & MAINTENANCE......................................................................................................................................... 82

17 CO2-O2-MODULE EXTRACTION................................................................................................................................... 83

18 PUMP EXTRACTION ......................................................................................................................................................... 85

19 SUPPORT ............................................................................................................................................................................. 89

20 TROUBLESHOOTING........................................................................................................................................................ 90

21 TECHNICAL SPECIFICATION ......................................................................................................................................... 91

21.1 LEO.............................................................................................................................................................................................................91

21.2 CO2-O2-MODULE ................................................................................................................................................................................92

21.3 T1-MODULE...........................................................................................................................................................................................93

21.4 T2-MODULE...........................................................................................................................................................................................93

22 FIGURE LIST........................................................................................................................................................................ 94

MANUAL REVISION TABLE ........................................................................................................................................................ 96

1Symbol description

This paragraph describes the symbols used in this manual and on the product label.

1.1 Symbols used in this manual and on the product

The following symbols identify important information:

CAUTION or WARNING or IMPORTANT: This symbol warns of circumstances or practices that

can affect the instrument’s functionality. Please refer to accompanying documents.

Tip ►Supplies you with helpful suggestions.

Note ►Supplies you with important information to successfully use the instrument.

1.2 Symbols on the product label

CE MARKING: This symbol indicates the product’s compliance with EU legislation.

PRODUCT DISPOSAL: this symbol indicates that this product must not be disposed as urban solid

waste.

This symbol indicates the product production date.

This symbol indicates the Manufacturer data.

This symbol warns you to read the user manual before starting the device.

This symbol indicates the protection degree against ingress of solids or liquids inside the product.

IP 40

2Safety Notes

In order to achieve maximum performance and to ensure proper operation of your new equipment, please

read carefully the following safety notes and the instructions. If you have any question, please contact Okolab.

−This equipment must be used only as intended and described in this Manual.

−This equipment should be operated only by qualified personnel.

−Do not use this equipment if any parts are damaged.

−This instrument is not intended for use in locations subject to flammable or explosive gases.

−Always transport equipment with care.

−The equipment and its internal parts may be damaged by dropping or shock.

−Do not use a volatile solvent, such as paint thinner, to clean the instrument, deformation or

discoloration will occur.

−Use a soft, dry cloth to remove stains from the instrument.

−Not following these instructions could damage the device and its accessories.

−Do not exceed the voltages indicated in this manual or on the product label.

−Avoid excessive induction noise, static electricity, or magnetic fields.

−Do not expose this instrument to water or moisture.

−Avoid throttling and kinking of tubes.

−Check tubing from time to time for possible wear and tear.

−Check that all tubing is fully inserted into connectors, and secure.

−This device is not designed for use in medical applications.

International caution symbol marks this device. It is important to read the “Safety Notes” before

installing, using and commissioning this device, as the notes contain important information relating

to safety and EMC. Not following these instructions can result in damage or breakdown of the device

and its accessories.

We reserve the right to make technical upgrades and changes.

IN NO EVENT, SHALL OKOLAB S.R.L. BE LIABLE FOR ANY DIRECT, INCIDENTAL, OR CONSEQUENTIAL

DAMAGES OF ANY NATURE –INCLUDING: LOSSES OR EXPENSES RESULTING FROM ANY DEFECTIVE PRODUCT,

OR THE USE OF ANY PRODUCT.

3Regulatory Compliance

3.1 Regulatory Statement

The product complies with the essential requirements of the LVD Directive 2014/35/EU and the EMC

Directive 2014/30/EU. The product has been tested and it complies with the requirements of the following

standards:

−European standard EN 61010-1: 2010 “Safety requirements for electrical equipment for

measurement, control and laboratory use”

−European standard EN 61326-1: 2013 “Electrical equipment for measurement, control and

laboratory use –EMC requirements”

The product meets the technical requirement of the Directive 2011/65/EU Restriction of the use of certain

hazardous substances (RoHS). The product has been tested according to European Standard EN 50581: 2012

“Technical documentation for the assessment of the electrical and electronic products with respect to the

restriction of hazardous substances”.

3.2 Waste Electrical and Electronic Equipment Directive (WEEE Directive)

The product must not be disposed as a general household waste. At the end of the product’s life, take the

product to a collection point designed for recovering and recycling of electrical and electronic devices.

In case of doubt, please return the product to Okolab s.r.l. for proper treatment.

4Preface

LEO (see Figure 1 for front and rear view of LEO) is a handheld meter for IVF Applications, allowing

measurement of CO2-O2 gas composition, temperature, relative humidity, gas flow of the high-top and bench-top

incubators, by using multiple sensor modules.

The CO2-O2-MODULE is embedded into LEO, and it is included standard. It measures, with a high accuracy,

the CO2-O2 volume concentration in the unit of %, and CO2-O2 partial pressure in the units of mbar, psi and KPa.

Other (external) sensor modules that can be connected to LEO’s connection ports are: T1-MODULE, T2-

MODULE, CO2-PPM-MODULE, HUMIDITY-MODULE, FLOW-RATE-MODULE. All sensor modules are calibrated by

using traceable standards, and store the calibration data in the internal memory. The sensor modules can be

delivered with traceable or accredited calibration certificate, and sent to Okolab for re-calibration. Moreover,

sensor calibration can be performed by using LEO intuitive user calibration routines.

LEO works in Diffusion Mode when the gas to be sampled is pressurized and in Aspiration Mode when it

works at atmosphere pressure, sucking the gas from the incubator by an internal pump. In both cases, if the gas

inside the incubator is humid, prior de-humidification with the Moisture Trap (included standard with LEO) is

needed to retain water molecules.

LEO can improve the CO2 and O2 reading accounting the water content of your incubator by use Wet

Mode. The temperature and relative humidity (RH) values of your incubator must be entered.

LEO can perform both single measurement and extended time logging, storing the data in its local memory.

LEO allows organization of the stored data, associating a label to each device. You can store up to 40 devices labels.

The stored data to be downloaded through the micro USB port, and analyzed by using the free LEO DATA IMPORT

Excel Macro.

LEO features a user-friendly 4.3” touch screen interface.

a b

Figure 1. LEO: front (a) and rear (b) view.

4.1 Sensor Modules

The available Sensor Modules (see paragraph 10 and paragraph 13.4)

−CO2-O2-MODULE (embedded into LEO)

−T1-MODULE (external)

−T2-MODULE (external)

−CO2-PPM-MODULE (external)

−HUMIDITY-MODULE (external)

−FLOW-RATE-MODULE (external)

Figure 2. LEO with T1-MODULE and T2-MODULE

5Supplied equipment

Figure 3 shows the Hard Case containing LEO’s equipment. The optional and spare parts can be purchased

from Okolab s.r.l. Please see www.oko-lab.com or contact info@oko-lab.com for more details.

Figure 3. LEO Hard Case.

A LEO. Hand-held meter for IVF applications.

B Power Supply (see paragraph 5.1).

C USB Cable.

D Soda Lime Kit (see paragraph 5.2).

E Moisture Trap, for humid gas measurement (see paragraph 5.3).

F Filter

G OTG Cable (see paragraph 5.4).

H Lanyard.

I Fitting Tubes (see paragraph 5.5).

J Tube-B (see paragraph 5.8).

K Tube-A (see paragraph 5.7).

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