Invitrogen Qubit 2.0 User manual
User Manual
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Qubit®2.0 Fluorometer
Catalog no. Q32866
Rev. Date: 4 October 2010
MAN0003231
2
Contents
Product Contents...........................................................................................................3
Safety Information ........................................................................................................4
Introduction..........................................................................................5
Description of Qubit®2.0 Fluorometer ......................................................................5
Product Specifications ..................................................................................................8
Methods................................................................................................9
Getting Started...............................................................................................................9
Guidelines for Using the Qubit®2.0 Fluorometer .................................................13
Calibrating the Qubit®2.0 Fluorometer...................................................................14
Reading Samples .........................................................................................................19
Calculating the Original Sample Concentration....................................................22
Cleaning and Maintenance........................................................................................29
Troubleshooting ..........................................................................................................30
Appendix ............................................................................................33
Critical Qubit™Assay Considerations.....................................................................33
Accessory Products.....................................................................................................36
Regulatory Requirements ..........................................................................................37
Technical Support .......................................................................................................38
Purchaser Notification................................................................................................39
3
Product Contents
Qubit®2.0
Fluorometer The Qubit®2.0 Fluorometer is shipped with the components
listed below:
Component Quantity
Qubit®2.0 Fluorometer 1
Universal Power Cord with 4 adaptor plugs
(for U.S./E.U./U.K./Australia) 1
Qubit®2.0 USB drive
(includes the instruction manual) 1
Qubit®2.0 Fluorometer Quick Reference Card
(QRC)
1
Certificate of Conformity (COC) 1
The complete instruction manual is also available on our website
at www.invitrogen.com/qubit.
See pages 5–8 for the description and specifications of the
Qubit®2.0 Fluorometer.
Upon Receiving
the Instrument Examine the instrument carefully for damage incurred during
transit. Ensure that all parts of the instrument, including
accessories listed above, are included with the product. Damage
claims must be filed with the carrier; the warranty does not cover
in-transit damage.
See page 9 to install the instrument.
Registering Your
Instrument Visit probes.invitrogen.com/qubit to register your instrument.
You will be asked to supply the serial number, your name, and
your contact details. Registering your instrument ensures that
you will receive notifications of software upgrades and
information on new assays for use with the Qubit®2.0
Fluorometer.
Intended Use For research use only. Not intended for any animal or human
therapeutic or diagnostic use.
4
Safety Information
Safety
Precautions Review and follow the safety instructions below.
Do not install the instrument in heavy humidity, such as in a
greenhouse or an incubator, to avoid a danger of electric
shock. If water or other material enters the instrument, the
adaptor, or power inlet, disconnect the power cord and
contact a service person. For operating environment, refer to
Product Specifications (page 8).
Do not touch the main plug or power cord with wet hands.
Always ensure that the power-supply input voltage matches
the voltage available in your location.
Do not install the instrument on a slant or a place prone to
vibrations; vibrations increase the risk of instrument
malfunction or damage.
Plug the power cord firmly into the wall outlet and AC
adapter.
To avoid potential shock hazard, make sure that the power
cord is properly grounded.
Be sure to position the equipment so that it is easy to
disconnect the instrument.
Turn off the instrument before unplugging the power cord
and/or moving the instrument.
If the instrument is broken or dropped, disconnect the power
cord and contact a service person. Do not disassemble the
instrument.
Use only authorized accessories (adaptor, power cord, and
USB drive).
5
Introduction
Description of Qubit®2.0 Fluorometer
Qubit®2.0
Fluorometer The Qubit®2.0 Fluorometer is a benchtop fluorometer for the
quantitation of DNA, RNA, and protein, using the highly sensitive
and accurate fluorescence-based Qubit™quantitation assays. Use
of the state-of-the-art dyes selective for dsDNA, RNA, and protein
minimizes the effects of contaminants in your sample that affect
the quantitation. Further, the very latest illumination and
detection technologies used in the Qubit®2.0 Fluorometer for
attaining the highest sensitivity allow you to use as little as 1 L of
sample and still achieve high levels of accuracy, even with very
dilute samples.
Features Important features of the Qubit®2.0 Fluorometer are:
Provides a user-friendly, benchtop design for simple, fast, and
highly accurate quantitation of DNA, RNA, and protein in
less than 5 seconds per sample (with sample incubation times
of 2 minutes for DNA and RNA, and 15 minutes for proteins).
Uses the Qubit™assays that contain advanced dyes that only
fluoresce when bound to DNA, RNA, or protein. This
specificity allows you to get very accurate results because
Qubit™technology only reports the concentration of the
molecule of interest, not contaminants.
Uses disposable assay tubes that eliminate washing steps and
cross contamination between samples.
Presents comprehensive data with graphic reports and a .CSV
(comma separated value) file for sample comparisons.
Continued on next page
6
Description of Qubit®2.0 Fluorometer, Continued
Instrument
Exterior
Components
The image below shows the location of the various parts of the
Qubit®2.0 Fluorometer.
Power Inlet connects the Qubit®2.0 Fluorometer to an electrical
outlet using the supplied power cord and the appropriate plug,
based on the electrical outlet configuration in your country.
Touchscreen located in the front of the instrument is the user
interface containing the buttons for all the functions needed and
displays data from the assays.
Sample chamber is used to insert the assay tube containing the
sample into the fluorometer for analysis.
USB port allows you to transfer and save data to your computer
for record keeping and printing purposes. The Qubit®2.0 USB
drive supplied with the instrument or any other standard USB
drive is inserted into the USB port for data transfer.
Continued on next page
7
Description of Qubit®2.0 Fluorometer, Continued
User Interface Use the touch screen of the Qubit®2.0 Fluorometer to operate the
instrument. The touch screen consists of:
The touch screen buttons to operate the instrument, including
alphanumeric buttons to enter sample names.
The digital display showing the data and graphs for the
standards and sample.
8
Product Specifications
Qubit®2.0
Fluorometer
Specifications
Environmental Conditions
Operating power: 100–240 VAC, 0.4 A
Frequency: 50–60 Hz
Electrical output: 9 VDC, 1.33 A
Installation site: Indoor use only
Operating temperature: 15–40°C
Maximum relative humidity: 20–80%, non-condensing
Instrument Specifications
Instrument type: Benchtop fluorometer
Instrument dimensions: 5.3” (w) × 8.5” (l) × 1.6” (h)
(13.3 cm × 21.3 cm × 4 cm);
oval shaped
Weight: 11.5 oz (340 g)
Dynamic range: 5 orders of magnitude
Processing time: ≤ 5 seconds/sample
Light sources: Blue LED (max ~470 nm)
Red LED (max ~635 nm)
Excitation filters: Blue 430–495 nm
Red 600–645 nm
Emission filters: Green 510–580 nm
Red 665–720 nm
Detectors: Photodiodes: measurement
capability from 300–1,000 nm
Calibration type: 2- or 3-point standard
Tube type: 0.5 mL Real Time PCR
(polypropylene) tubes
Warm-up time: 2 seconds
Qubit®2.0 USB Drive: 2 Gigabyte
9
Methods
Getting Started
Installing the
Qubit®2.0
Fluorometer
The Qubit®2.0 Fluorometer is a stand-alone instrument that does
not require a connection to a computer.
1. After unpacking the instrument, place the instrument on a flat,
level, dry surface.
2. Plug one end of the supplied power cord into the Qubit®2.0
Fluorometer. To the other end attach the appropriate plug
adaptor, based on the electrical outlet configuration in your
country.
3. Plug the power cord into the electrical outlet. Be sure to use
only the power cord supplied with your instrument. Powering
the instrument with an unapproved power cord may damage
the instrument.
4. The instrument is automatically powered on when it is
plugged in.
5. To power down the Qubit®2.0 Fluorometer, unplug the
instrument.
Continued on next page
10
Getting Started, Continued
Sleep Mode The Qubit®2.0 Fluorometer has an auto-standby (i.e., sleep) mode
that is triggered after 30 minutes of inactivity. You can also trigger
the stand-by mode by touching the Power button ( ) located on
the top left of the touch screen.
To recover from the standby mode, touch anywhere on the touch
screen. Recovery from standby mode returns you to the same
screen present when sleep mode commenced. After 7.5 hours of
inactivity, touching the screen returns you to the Home Screen
(see below).
Home Screen When the instrument is turned on, the Home Screen is displayed.
From the Home Screen, you can proceed immediately to the
quantitation assays (DNA, RNA, or protein), choose to calibrate
the instrument with the appropriate standards, access the saved
data, set up the date and time, or adjust the screen brightness.
Continued on next page
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