Hanna Instruments HI97727 User manual

INSTRUCTION MANUAL

HI97727

Color of Water

Photometer

Thank you for choosing a Hanna Instruments product.

Please read this instruction manual carefully before using the

instrument.

This manual will provide you with the necessary information for correct

use of the instrument, as well as a precise idea of its versatility.

If you need additional technical information, do not hesitate to

e‑mail us at [email protected] or view our worldwide contact

list at www.hannainst.com.

All rights are reserved. Reproduction in whole or in part is prohibited without the written consent

of the copyright owner, Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

Dear

Customer,

TABLE OF CONTENTS

1. PRELIMINARY EXAMINATION ........................................................................

2. SAFETY MEASURES......................................................................................

3. SPECIFICATIONS..........................................................................................

4. ABBREVIATIONS..........................................................................................

5. DESCRIPTION..............................................................................................

5.1. GENERAL DESCRIPTION AND INTENDED USE...........................................

5.2. FUNCTIONAL DESCRIPTION....................................................................

5.3. PRECISION AND ACCURACY....................................................................

5.4. PRINCIPLE OF OPERATION.....................................................................

5.5. OPTICAL SYSTEM ..................................................................................

6. GENERAL OPERATIONS.................................................................................

6.1. METER VALIDATION: CAL CHECK/CALIBRATION ........................................

6.2. GLP .....................................................................................................

6.3. LOGGING DATA/LOG RECALL...................................................................

6.4. GENERAL SETUP...................................................................................

6.5. CONTEXTUAL HELP................................................................................

6.6. BATTERY MANAGEMENT........................................................................

7. COLLECTING AND MEASURING SAMPLES........................................................

8. METHOD PROCEDURE ..................................................................................

9. ERROR DESCRIPTIONS .................................................................................

10. BATTERY REPLACEMENT .............................................................................

11. ACCESSORIES............................................................................................

CERTIFICATION................................................................................................

RECOMMENDATIONS FOR USERS......................................................................

WARRANTY .....................................................................................................

1. PRELIMINARY EXAMINATION

Remove the instrument and accessories from the packaging and examine it carefully to

make sure that no damage has occurred during shipping. Notify your nearest Hanna

Customer Service Center if damage is observed.

Each HI97727C is supplied with:

• Sample cuvette (2 pcs.)

• Sample cuvette cap (2 pcs.)

• Plastic stopper (2 pcs.)

• A ZERO ‑ CAL Check Cuvette A

• HI97727B ‑ CAL Check Cuvette B for Color of Water

• Cloth for wiping cuvettes

• 1.5V AA Alkaline batteries

• Instruction manual

• Meter quality certificate

• CAL Check standard certificate

Each HI97727 is supplied with:

• Sample cuvette (2 pcs.)

• Sample cuvette cap (2 pcs.)

• Plastic stopper (2 pcs.)

• 1.5V AA Alkaline batteries

• Instruction manual

• Meter quality certificate

Note: Save all packing material until you are sure that the instrument works correctly.

Any damaged or defective item must be returned in its original packing material with the

supplied accessories.

2. SAFETY MEASURES

• The chemicals contained in the reagent kits may be hazardous if

improperly handled.

• Read the Safety Data Sheets (SDS) before performing tests.

• Safety equipment: Wear suitable eye protection and clothing when required, and

follow instructions carefully.

• Reagent spills: If a reagent spill occurs, wipe up immediately and rinse with plenty

of water. If reagent contacts skin, rinse the affected area thoroughly with water.

Avoid breathing released vapors.

• Waste disposal: For proper disposal of reagent kits and reacted samples, contact

a licensed waste disposal provider.

PRELIMINARY EXAMINATIONSAFETY MEASURES

Color of

Water

Range 0 to 500 PCU (Platinum Cobalt Units)

Resolution 1 PCU

Accuracy ±10 PCU ±5% of reading at 25 °C

Method

Adaptation of the Standard Methods

for the Examination of Water and

Wastewater, 18th edition, Colorimetric

Platinum Cobalt method

Measurement

System

Light source Light Emitting Diode

Bandpass filter 420 nm

Bandpass filter

bandwidth 8 nm

Bandpass filter

wavelength accuracy ±1.0 nm

Light detector Silicon photocell

Cuvette type Round 24.6 mm diameter (22 mm inside)

Additional

Specifications

Auto logging 50 readings

Display 128 x 64 pixel B/W LCD with backlight

Auto‑off After 15 minutes of inactivity

(30 minutes before a READ measurement)

Battery type Alkaline 1.5 V AA (3 pcs.)

Battery life > 800 measurements (without backlight)

Environment 0 to 50 °C (32 to 122 °F);

0 to 100% RH, non‑serviceable

Dimensions 142.5 x 102.5 x 50.5 mm (5.6 x 4.0 x 2.0")

Weight (with batteries) 380 g (13.4 oz.)

Case ingress protection

rating IP67, floating case

3. SPECIFICATIONS

SPECIFICATIONS

4. ABBREVIATIONS

mg/L milligrams per liter (ppm)

mL milliliter

°C degree Celsius

°F degree Fahrenheit

LED Light Emitting Diode

HDPE High Density Polyethylene

GLP Good Laboratory Practice

PCU Platinum Cobalt Units

DIW Deionized Water

NIST National Institute of Standards and Technology

ABBREVIATIONS

DESCRIPTION

5. DESCRIPTION

5.1. GENERAL DESCRIPTION AND INTENDED USE

The HI97727 is an auto‑diagnostic portable meter that benefits from Hanna’s years

of experience as a manufacturer of analytical instruments. It has an advanced optical

system that uses a Light Emitting Diode (LED) and a narrow band interference filter

that allows for accurate and repeatable readings.

The optical system is sealed from outside dust, dirt and water. The meter uses an

exclusive positive‑locking system to ensure that the cuvettes are placed into the holder

in the same position every time.

With the CAL Check functionality, users are able to validate the performance of the

instrument at any time and apply a user calibration (if necessary). Hanna's CAL Check

cuvettes are made with NIST traceable standards.

The built in Tutorial mode guides users step‑by‑step through the measurement process. It

includes all steps required for sample preparation, the required reagents and quantities.

The HI97727 meter measures color of water samples from 0 to 500 PCU. The method is

an adaptation of the Standard Methods for the Examination of Water and Wastewater,

18th edition, Colorimetric Platinum Cobalt method.

By comparison with known color of platinum cobalt chloride standards method is used

to determine “apparent color” of water (color from suspended and dissolved components

measured using un‑filtered samples) and “true color” (measured using filtered samples

after removal of slightly turbidity).

The HI97727 photometer is a compact and versatile meter suitable for field or bench

measurements, featuring a:

• Sophisticated optical system

• Meter validation using certified CAL Check cuvettes

• Tutorial Mode guides the user step‑by‑step

• Auto logging

• Waterproof IP67, floating case

• GLP Features

Keypad Description

The keypad contains 3 direct keys and 3 functional keys with the following functions:

Press the functional keys to perform the function displayed above them on the LCD.

Press and hold to power off/on. Press briefly to return to the previous screen.

Press to access the menu screen.

Press to display the context‑sensitive help menu.

DESCRIPTION

1) ON/OFF power button 3) Liquid Crystal Display (LCD) 5)Indexing mark

2) Keypad 4) Cuvette holder 6) Battery cover

5.2. FUNCTIONAL DESCRIPTION

DESCRIPTION

5.4. PRINCIPLE OF OPERATION

Absorption of light is a typical phenomenon of interaction between electromagnetic

radiation and matter. When a light beam crosses a substance, some of the radiation

may be absorbed by atoms, molecules or crystal lattices.

If pure absorption occurs, the fraction of light absorbed depends both on the optical

path length through the matter and on the physical‑chemical characteristics of the

substance according to the Lambert‑Beer Law:

‑log I/Io= elc d

A = elc d

Io= intensity of incident light beam

I = intensity of light beam after absorption

el = molar extinction coefficient at wavelength l

c = molar concentration of the substance

d = optical path through the substance

Therefore, the concentration "c" can be calculated from the absorbance of the substance

as the other factors are constant.

Photometric chemical analysis is based on specific chemical reactions between a

sample and reagent to produce a light‑absorbing compound.

5.3. PRECISION AND ACCURACY

Precision is how closely repeated

measurements are to one another.

Precision is usually expressed as

standard deviation (SD).

Accuracy is defined as the

closeness of a test result to the

true value.

Although good precision suggests

good accuracy, precise results can

be inaccurate. The figure explains

these definitions.

For each method, the accuracy is expressed in the related measurement section.

DESCRIPTION

Instrument Block Diagram

The internal reference system (reference detector) of the HI97727 photometer

compensates for any drifts due to power fluctuations or ambient temperature changes,

providing a stable source of light for your blank (zero) measurement and sample

measurement.

LED light sources offer superior performance compared to tungsten lamps. LEDs have

a much higher luminous efficiency, providing more light while using less power. They

also produce little heat, which could otherwise affect electronic stability. LEDs are

available in a wide array of wavelengths, whereas tungsten lamps have poor blue/

violet light output.

Improved optical filters ensure greater wavelength accuracy and allow a brighter,

stronger signal to be received. The end result is higher measurement stability and less

wavelength error.

A focusing lens collects all of the light that exits the cuvette, eliminating errors from

cuvette imperfections and scratches, eliminating the need to index the cuvette.

5.5. OPTICAL SYSTEM

GENERAL OPERATIONS

To perform a CAL Check:

1. Press CAL Check key from measurement mode.

The “Not Available“ message or the date/time

and status of the last CAL Check will be displayed

on the screen.

2. Press the Check key to start a new CAL Check.

Press the key at any time to abort the

validation process.

3. Use the keys to enter the certificate value

of the calibration standard found on the CAL Check

Standard Certificate. Press Next to continue.

Note: This value will be saved in the instrument for future validation. If a new

set of calibration standards is obtained please update the certificate value.

4. Insert the A ZERO CAL Check Cuvette A then press

Next to continue. The “Please Wait...“message

will be displayed during the measurement.

5. Insertthe HI97727B CALCheck CuvetteB then press

Next to continue. The “Please Wait...“message

will be displayed during the measurement.

6.1. METER VALIDATION: CAL CHECK / CALIBRATION

Validation of the HI97727 involves verifying the concentration of the certified CAL

Check standards. The CAL Check screen guides the user step‑by‑step through the

validation process and user calibration (if necessary).

WARNING: Do not use any solutions/standards other than the HANNA®CAL Check

Standards. For accurate validation and calibration results, please perform these at

room temperature (18 to 25 °C; 64.5 to 77.0 °F).

Note: CAL Check Standards will not read the specified value in measurement mode.

Protect the CAL Check cuvettes from direct sunlight by keeping them in the original

packing. Store between +5 °C and +30 °C (41 - 86 °F), do not freeze.

6. GENERAL OPERATIONS

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