Formulatrix MANTIS User manual
Original Instructions
Applies to Continuous Flow, LAM, ACC, LC3, RFID models
January 2018
MAG-V40R018
© 2018 FORMULATRIX. All Rights Reserved.
User’s Guide
MANTIS®Liquid Handler
FORMULATRIX Product Information
Formulatrix.com
FORMULATRIX Corporate Headquarters
10 DeAngelo Drive, Bedford, MA 01730 USA Phone: +1 781 788 0228
For information requests, email [email protected]. For sales inquiries, email [email protected]. For support,
email [email protected]. To comment on FORMULATRIX documentation, email publications@formulatrix.com.
FORMULATRIX European Representative
Carl Pedersen
Support, Europe
Phone: +44 7805512001
Table of Contents
Chapter 1: Introduction.....................................................................................................................................................................................................................................1
Symbols and Conventions.................................................................................................................................................................................................................................................2
Chapter 2: Safety Information.......................................................................................................................................................................................................................3
Introduction...................................................................................................................................................................................................................................................................................3
Regulatory Compliance.......................................................................................................................................................................................................................................................3
Equipment Safety Guidelines..........................................................................................................................................................................................................................................4
Mechanical Hazards................................................................................................................................................................................................................................................................4
Electrical Hazards.....................................................................................................................................................................................................................................................................4
Chapter 3: Specifications................................................................................................................................................................................................................................5
Physical Dimensions...............................................................................................................................................................................................................................................................5
Computer Requirements....................................................................................................................................................................................................................................................7
Electrical Requirements.......................................................................................................................................................................................................................................................7
Chapter 4: Overview..........................................................................................................................................................................................................................................8
Key Features................................................................................................................................................................................................................................................................................8
Chapter 5: Hardware Overview..................................................................................................................................................................................................................10
MANTIS Components.........................................................................................................................................................................................................................................................10
Chapter 6: Initial Setup...................................................................................................................................................................................................................................13
Setting Up the MANTIS......................................................................................................................................................................................................................................................13
Adding Plate Types to the Plate Type Library.................................................................................................................................................................................................14
Adding Circular Plates to the Plate Type Library...........................................................................................................................................................................................23
Adding Stock Reagents to the Reagent List.....................................................................................................................................................................................................26
Calibrating the Chip Port Positions...........................................................................................................................................................................................................................27
Calibrating Continuous Flow Chips..........................................................................................................................................................................................................................27
Default Volume and Tubing Length Recommendation...........................................................................................................................................................................28
Chapter 7: Software Overview...................................................................................................................................................................................................................30
Main Software Overview..................................................................................................................................................................................................................................................30
Device Control Panel...........................................................................................................................................................................................................................................................32
Input Control Panel...............................................................................................................................................................................................................................................................35
Dispense List Designer.......................................................................................................................................................................................................................................................41
Well Selection Patterns.....................................................................................................................................................................................................................................................43
Status Bar.....................................................................................................................................................................................................................................................................................43
Chapter 8: Setting Up the Hardware.......................................................................................................................................................................................................44
Chapter 9: Dispensing With the MANTIS...............................................................................................................................................................................................47
Designing a Dispense.........................................................................................................................................................................................................................................................47
Designing a Multi-Plate Dispense.............................................................................................................................................................................................................................49
iii
iv
Viscous Dispensing Tips and Tricks.........................................................................................................................................................................................................................51
Adding a Dispense Delay.................................................................................................................................................................................................................................................53
Using Excel to Edit a Dispense Design..................................................................................................................................................................................................................53
Creating and Using Protocols.......................................................................................................................................................................................................................................54
Creating and Using Reagent Configurations....................................................................................................................................................................................................55
Priming the Chips...................................................................................................................................................................................................................................................................56
Executing a Plate Dispense............................................................................................................................................................................................................................................57
Recovering Excess Reagent Volume.....................................................................................................................................................................................................................58
Importing Dispense Data..................................................................................................................................................................................................................................................59
Reloading Dispense Data................................................................................................................................................................................................................................................68
Normalizing Concentration............................................................................................................................................................................................................................................69
Cleaning the Chips................................................................................................................................................................................................................................................................73
Dispensing ROCK MAKER®Crystallization Software Plate Designs...............................................................................................................................................74
Dispensing FORMULATOR®Screen Builder Plate Designs..................................................................................................................................................................75
Performing a MANTIS Factory Reset......................................................................................................................................................................................................................76
Resolving Dispense List Errors....................................................................................................................................................................................................................................77
Using the Options Menu...................................................................................................................................................................................................................................................78
Using the Security Settings............................................................................................................................................................................................................................................83
Chapter 10: Large Capacity Chip Changer (LC3)...............................................................................................................................................................................88
LC3 Components...................................................................................................................................................................................................................................................................88
Setting Up the LC3 for a Dispense............................................................................................................................................................................................................................91
Tuning the LC3 Carousel..................................................................................................................................................................................................................................................92
Chapter 11: MANTIS Radio-Frequency Identification (RFID)........................................................................................................................................................95
Using the RFID Functionality in MANTIS..............................................................................................................................................................................................................97
Chapter 12: MANTIS Configuration........................................................................................................................................................................................................101
Configuring MANTIS-ROCK MAKER Integration..........................................................................................................................................................................................101
Configuring MANTIS-FORMULATOR Integration.......................................................................................................................................................................................104
Chapter 13: Deck Geometry Correction..............................................................................................................................................................................................106
Chapter 14: System Maintenance...........................................................................................................................................................................................................108
Sterilizing MANTIS Chips ..............................................................................................................................................................................................................................................108
Chapter 15: Troubleshooting and FAQs...............................................................................................................................................................................................110
Troubleshooting....................................................................................................................................................................................................................................................................110
FAQs...............................................................................................................................................................................................................................................................................................114
Accessories........................................................................................................................................................................................................................................................115
Index......................................................................................................................................................................................................................................................................117
1
Introduction
Chapter 1
Thank you for purchasing the MANTIS, a low dead-volume, non-contact liquid dispenser.
The unique design of this small machine minimizes dead volume and accepts pipette tip or
tube inputs.
Your MANTIS must be used in the manner described in this user’s guide. Any other use may
result in damage to your MANTIS or personal injury. FORMULATRIX, Inc. is not responsible
in whole or in part for any damages caused by: improper use, unauthorized alterations,
adjustments or modifications, failure to comply with the procedures outlined in the MANTIS
User’s Guide, or use of the products in violation of applicable laws, rules, or regulations.
Except as otherwise expressly provided in this user guide, any alteration, adjustment, or
modification to the products will void the product warranty.
Read, understand, and observe all safety information and instructions in this manual before
using your MANTIS. Please note specific safety requirements as explained in this user’s guide.
Failure to follow these instructions could result in serious personal injury.
Please save this guide for future reference and have it available to all operating personnel.
The MANTIS is manufactured to the current technical safety-relevant regulations.
2
This icon denotes a caution and advises you of precautions to take.
This icon denotes a prohibited action.
This icon denotes a compulsory action and advises you of actions
you must take.
This icon denotes a caution relating to electric shock and advises you
of precautions to take.
This icon denotes a fire hazard and advises you of precautions to
take.
This icon denotes a warning.
Bold text denotes items you must select or click in the software, such
as menu items and dialog box options. Bold text also denotes
parameter names.
bold
Text in this font denotes text or characters you should type from the
keyboard.
monospace
Symbols and Conventions
MANTIS User's Guide | Chapter 1: Introduction
3
The safety recommendations in this guide are basic guidelines. If the lab where the
MANTIS is to be kept has additional safety guidelines they should be followed as well,
along with all applicable national and international safety codes.
FORMULATRIX products are CE (Conformité Européenne) Machinery, Low Voltage, and
EMC directive compliant. If necessary, request the official Declaration of Conformity
(DOC) from FORMULATRIX.
Safety Information
Chapter 2
Introduction
Only FORMULATRIX personnel are permitted to transport, assemble, or service the
Use only parts supplied or recommended by FORMULATRIX in this document or via
email.
Regulatory Compliance
4
üRead all safety and operating instructions before operating the MANTIS.
üDo not place the MANTIS connections (power, waste lines, and communications
cables) where they could cause a safety hazard.
üDo not place the product in a location where it may be subject to physical damage.
üEnsure that all power connections to the product are properly grounded.
üDo not remove the nameplate, or any warning, hazard, or Equipment Identification
labels.
üTurn OFF power before inserting or removing power cables.
The MANTIS is a complex electromechanical instrument. Only persons with the proper
training should service or operate the product.
All facilities to the product must be disconnected before servicing, or injury may result
from the automatic operation of the equipment.
The proper precautions for operating electrical equipment must be observed.
Equipment Safety Guidelines
Mechanical Hazards
Electrical Hazards
Avoid damage to the power cables. Do not apply unnecessary stress, place heavy objects
on, or crush the cables or fire may occur.
Do not use extension cables unless approved by FORMULATRIX.
Switch off all power sources before removing connectors or electric shock may occur.
Arrange cables in accordance with the Technical Standard for Electric Properties and
Extension Rules or fire may occur.
MANTIS User's Guide | Chapter 2: Safety Information
5
MANTIS
●Width: 354 mm or 13.9” (smaller without ACC or reagent holders)
●Height: Full Extension: 226 mm or 8.9” (not including pipette tip input)
●Height: No Extension: 193 mm or 7.6” (not including pipette tip input)
●Depth: 212 mm or 8.3”
Specifications
Chapter 3
Physical Dimensions
Front Side
226 mm
354 mm 212 mm
6
Large Capacity Chip Changer (LC3)
●Width: 240 mm or 9.4”
●Height: Full Extension: 284 mm or 11.2”
●Height: No Carousel: 88 mm or 3.5”
●Depth: 240 mm or 9.4”
Front Side
MANTIS User's Guide | Chapter 3: Specifications
MANTIS with Large Capacity Chip Changer (LC3)
●Width: 449 mm or 17.7” (MANTIS with LC3)
●Height: Full Extension: 284 mm or 11.2” (not including pipette tip input)
●Depth: 240 mm or 9.4”
Front Side
284 mm
240 mm 284 mm
449 mm
284 mm
240 mm
Table of contents
