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User’s ManUal
Concentric Microdialysis Probe
Model AZ-X-Y
• AllEicomproductsareforanimalresearchpurposeonly.
• Keeptheprobeawayfromphysicalshock.
• Inletneedleislongerthanoutletneedle.
• Duramustbebrokenpriortoinsertionintothebrain.
AZ-X-Y Probe
the probe membrane. After flushing for several minutes (it is not
necessarytoflushforanextendedperiodoftime)themembranemay
“sweat”orappearwetthisisnormal,notduetodamageoraclog.
2. Ifnoflowisobservedviatheoutletneedle,soaktheprobeindistilled
water for a few minutes and repeat instructions in item 1. After
conrmingthedistilledwaterflowfromtheoutlet,pleaseremovethe
probefromplasticsheathandconrmnoleaking.
3. We test each probe for defect/leaking prior to shipping. If you nd
adefectiveprobeafterfollowingtheabovestepsEicomwillreplace
theprobeatnocharge.Wedonotguaranteeprobesafterinsertion
toanimals.
Removing Air Bubble
1. Ifthereisanairbubbleintheprobe,pleaseremoveit.Becausethe
airbubblewillcovertheactivemembranesiteandkeeptheperfusate
away.Thiswillresultinalowerrecoveryrate.Theairbubbleshouldbe
removedbeforestudy.Ifitissmall,itwilldisappearincaseofinvivo
studybutmaynotinvitro.
2. Perfusateneedstobedegassedbeforeuse.
3. Toremovetheairbubble,pleaseintroduceabigairtotheinletand
flushout.Donotconnectoutlettubingatthistime.Thebigairbubble
willtrapandremoveasmallairbubble.
Connecting Outlet
1. Conrmneitherleakingorairbubblesarepresentinthemembrane.
Settheflowrateonthesyringepump.Astandardflowrateis1-2μl/
min.Ahighflowratecoupledwithalongoutlettubingcanresultin
damageoftheprobemembrane.
2. Connect Teflon tubing (model JT-10) or other tubing to the outlet.
A long tubing length (more than 150 cm) can cause leaking of the
membraneortheliquidswivelduetotheinducedhighpressure.Ifyou
usetubinglongerthan150cm,pleaseavoidusingflowrateshigher
than 2 μl/min. Polyethylene tubing can cause confounding peaks
whenanalyzingsamplesusingHPLCwithelectrochemicaldetection.
Cleaning Probes and Storage
Removetheprobefromthesystemandflushwithdistilledwatertowash
out salts. Then put the probe in the distilled water and place at 4°C for
storage. Keep the membrane wet to prevent itfrom shrinking. Keep the
probeawayfromphysicalshock.
Cleaning Lines (Teflon, FEP tubing)
Bacteriagenerationandproteinpollutionoftubingcanbeacauseoflow
recoveryrates,especiallyintheoutlettubing.Inordertoavoidthisproblem,
washthelineswith50timesdilutedcommercialkitchenbleach(chlorine)in
purewater.Perfusethesolutionintothelines(nottheprobe)andleaveitfor
atleastonehour,thenthoroughlyrinsewithwater.
Perfusion Solution
Wearecommendusinganon-bufferedaCSFsolutionbecausephosphate
buffersolutionsmayprecipitatecalciumphosphateandcanresultinclogs.
Probe Check
1. Injectdistilled water using a1 or 2 mldisposable (plastic) syringe.
Connect the syringe to the inlet (longer needle) of the probe using
Bitontubing(JB-30).Theprobemaybecomecloggedordamagedif
youinitiallyflushwithasaltsolution.Werecommendusingdistilled
waterfortheinitialprobeflush.Donotconnecttubingtotheoutlet
oftheprobeduringflushing.Pleaseconrmtherearenoleaksfrom
AD12-0718
inner 0.105/0.040 mm PFS
outer 0.63/0.33 mm SUS
inner 0.150/0.075 mm PFS
outer 0.63/0.33 mm SUS
0.3/0.2 mm SUS
0.18/0.12 mm SUS
Inner Dead Volume
inlet: 0.05 µL
outlet: 0.4 µL
9.0 mm
12.0 mm
X + 5
PFS: Polyimide-Resin
Coated Fused Silica
Membrane: Artificial Cellulose
Cut off: MW 50,000
OD/ID: 0.22/0.20 mm
SUS (Stainless Steel) 316
Y
Model: AZ-X-Y
X = (guide cannula length)
Y = (membrane length)
