Drawell K960-A User manual
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K960 GRADIENT
THERMAL CYCLER
OPERATING MANUAL
Drawell International Technology Limited
Chongqing Drawell Instrument CO,.Ltd
Add:Suite 2705,Building No.12,Shiyou Road No.1,Yuzhong District,
Chongqing,China
Tel: 0086-023-63268643
Shanghai Drawell Scientific Instrument Co.,Ltd
Add : Suite 1117,Lane561 XiuChuan Rd.,PuDong New
Area,Shanghai,China
Web : www.drawell.com.cn
Email : sales05@drawell.com.cn
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Contents
1.
Important Note................................................................................2
1. 1 Unpacking check...................................................................................................................2
1. 2 Security Warning................................................................................................................... 2
1.2.1 Safe operation.................................................................................................................. 3
1.2.2 Maintenance of security...................................................................................................3
2. Instrument Introduction................................................................. 4
2.1 PCR Technical Summary........................................................................................................4
2.2 Features.....................................................................................................................................5
2.2.1 Power-on intro..................................................................................................................5
2.2.2 Software intro...................................................................................................................5
2.2.3 Structure intro...................................................................................................................6
2.2.4 Button intro....................................................................................................................12
2.2.5 Instrument working condition and performence indicators........................................... 13
3. Menu Operation.............................................................................14
3.1 File Operation.......................................................................................................................14
3.1.1 New 14
3.1.2 Edit. 16
3.1.3 Copy 16
3.1.4 Delete 16
3.2 Parameter setting...................................................................................................................17
3.2.1 Operating parameter setting...........................................................................................17
3.2.2 heat lid setting................................................................................................................17
3.2.3 System setting................................................................................................................18
3.3 Information inquiry...............................................................................................................18
3.3.1 Log inquiry.....................................................................................................................18
3.3.2 Gradient inquiry.............................................................................................................19
3.3.3 Help information............................................................................................................19
4. Common Failure Analysis.............................................................21
4.1 Failure code and corresponding phenomenon...................................................................... 21
4.2 Common failure analysis and corresponding solving method............................................. 21
4.2.1 Bad experiment results.................................................................................................. 21
4.2.2 Failure reason analysis and corresponding solving method.......................................... 22
5. Instrument Warranty....................................................................23
5.1 Warranty content...........................................................................................................23
5.2 Warranty reference........................................................................................................23
6. Question and Answering............................................................... 24
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1.
Important Note
1.1 Unpacking check
When you received the K960 Thermal Cycler, please open the package and check whether the
items listed below are contained:
1. Thermal cycler ( one block included )
2. Power supply line ( single-phase three threads)
3. Instrument direction
4. Instrument warranty card
5. Instrument qualification
6. Instrument packing list
7. Instrument factory testing report
8. Two tablets of spare fuse ( AC 250V 10A)
9. Other scheduled components
If you find the components in the box are not consistent with
the list, please contact us as soon as possible, and our company
will try our best to solve the problem.
Note:When you receive the K960 and find the package seriously impaired or the
instrument impaired, please keep the package and contact us immediately, we will replace
the product and find out the reason.
1.2 Security Warning:
Please carefully read the manual before operating the instrument, otherwise the improper
operations may cause damage to the instrument or harm your personal safety
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1.2.1 Operating safety
A.C. power’s grounding should be reliable for fear of an electric shock.
The 3-pin plug with this instrument’ s power line is a safety device that
should be matched with a grounded socket. Before turn on the power,
always check if the mains voltage is consistent with the instrument required.
Ensure the rated load of socket is not lower than required.
If the A.C. power line is damaged, it must be replaced with a new one.
The power line should be free of heavy objects during the instrument’s
operation. Keep the power line away from the place where people gather
regularly. While connect or disconnect the power line, you should hold the 3-
pin plug with your hand. Insert the plug thoroughly to ensure good contact
between the plug and socket. Pull the plug, not the line, when you need to
disconnect to the mains.
During instrument operation, the metal part and the hotlid can reach
hightemperature, may cause burns and liquid boil over，is not allowed to be
touchedby any part of the body for fear of the body burn, during the whole
operation.
Don’t put anything around the instrument closer than 30 cm when it is
working to ensure the heat dispersion.
1.2.2 Maintenance safety
The instrument need timely clean the block and the heat lid to keep the experiment
precise. It is advised that you take out the temperature changing block before you clean.
Use a piece of clean soft cloth with some absolute alcohol to swap lightly. Corrosive scour
is not allowed, and you should never drop the scour into the heat lid. Put back the block
after cleaning.
Once one of the following events occurs, you are suggested to
disconnect the power line with mains, and contact the distributor or ask a
certificated maintenance worker for help：
●Reagent, rain, or other liquid into the device;
●The device malfunctioning, giving off abnormal sound or odor, or the
screen displays Error!
●The device falling from high place onto the floor or its shell damaged;
●Significant changes in the device’s performance, such as can’t power
on or operate normally.
Note: For your safety and the normal operating of the instrument, don’t open the
instrument. Otherwise your safety is not guaranteed and you will lost the right to warranty.
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2.
Instrument Introduction
2.1 PCR technique brief intro
Polymerase Chain Reaction (PCR) is a technique which is used to amplify the number
of copies of a specific region of DNA developed in middle of 80s. There are many distinct
merits in the PCR technology, such as specialization, sensitivity, high productivity, celerity,
convenience, good reproductively, automatization and so on. In a reaction tube, target
gene or segment of DNA, in a matter of hours, will be amplified a million fold, and can be
seen by naked eyes; from a hair, a drop of blood, even a cell can be amplified to enough
number of DNA for analyzing and identifying. In the past, the procedure may take days
and weeks, but now it only take a few hours. PCR is a revolutionary innovation and
landmark in the field of biological medicine.
PCR has been widely applied in immunology, human genome project, forensics,
oncology, population biology, paleontology ，zoology ，botany research field and clinical
diagnosis of virus, tumor, hereditary disease, etc. , by PCR detection.
As the gradient PCR becoming the optimal method of optimizing the reaction condition,
Jingle gradient has become the standard of modern molecule laboratory.
A freely controlled grads temperature can not only optimize the anneal temperature but
also optimize all the temperature of each PCR step, even the most stringent application.
Thanks to the application of the semi-conductor, an optimal, constant increasing and
decreasing temperature can be assured, which means the gradient experiment results
can be used easily and precisely in common application, decreasing the intensity of the
exploring experiment and increase the quality and efficiency of the experiment.
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2.2 Features
2.2.1 Power on intro
After connecting to the electricity, turn on the power, the instrument will beep, that
means the power have been supplied, and the LCD will display starting windows(see
fig.1), and the device will have self-testing, after the self-testing is passed, the device is
ready for operation(see figure 2).This demonstrate the instrument is normal and we can
begin the experiment. If the LCD don’t light on or something abnormal happens, please
cut of the power supply and connect the producer or get it repaired according to chapter 5
in this manual.
2.2.2. Software intro
This system provides us with a customer selection programming platform. It can held the
temperature at constant degree, it can also realize quick temperature adjusting between
different temperature set point. The block is designed to be substitutable, which enable
the customers to select different blocks according to their own demand, such as
96*0.2mL,54*0.5 mL 、96*0.2mL+77*0.5 mL 、384well, providing sufficient experiment
sample.
Running in a low temperature is the basic feature of the advanced gene amplification
instrument. Its ability to deal with the sample in low temperature，together with the PCR
itself, make it possible that all procedure can be done automatically. This system uses a
advanced and reliable semi-conductor refrigeration scheme, providing an efficient
temperature adjusting speed and super wide temperature controlling scale.
Referring to the software designing , the 320*240 super big LCD display can display
complete information simultaneously. All buttons are labeled by numbers, making the
programming quick and easy. This system slam the door of the link method in the tradition
programming method, making use of the specified nature programming method. Link
method is hard to learn and command, and the saved programming segment is easy to be
damaged.
30 ℃-99 ℃super long grads range, furnishing the customer more experiment authority,
and get results of different temperature in one experiment, decreasing the cost and the
intension of the experiment. Particularly to some experiment with precise temperature and
uncertainty, the operator can adjust the temperature flexibly, make sure to achieve the
efficient experiment.
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2.2.3 Structure intro
2.2.3.1 appearance view
⑴
⑶
⑷
⑸
⑹
⑵
⑺
⑻
⑼
⑽
⒁
⒂
⒃
⑾
⑿
⒀
⒄
⒇
⒅
⒆
1 heat dispersion hole 2 bottom 3 eet cushion 4 air in hole 5 upper cover
6 button panel 7 display 8 button 9 switch 10 power supply socket 11 Fuse
socket 12 network interface 13 USB interface 14 232 interface 15 shell 16 air out
hole 17 LOGO 18 heat lid spanner 19 grade knob 20 grade calibration
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Draw the
spanner and
push
it
2.2.3.2 free adjusting angle between the upper cover and the shell
This instrument uses the gemel connecting technique and enables the upper cover to
stay at any angle, solving the problem brought by the traditional spring connecting
structure such as unreliable, instability and short using time.
stay at any
angle according
to your demand
As the above figure shows, when you draw the spanner, the upper cover come away from
the shell and could be fixed at any angle. The personalize structure makes the product
more beautiful and more convenient to use for the operator.
Note: The angle between the upper cover and the shell range from 0 to 90, please don’t
push it more than the upper limit 90 so as not to break the instrument.
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2.2.3.3 selectable heat lid calibration
Due to the demand of the experiment, we need to use different test tubes, so we
need different instrument suit the tests. This instrument has a design, with which the
operator can meet the need of different test tubes on the same instrument simply by
changing different block assembly.
As the above figures show, the calibrations 0.5,0.2,0.2and384 on the panel
correspondingly suits the 0.5ml,0.2ml(dome),0.2ml(flat) and 384well test tube. Suppose
you have to use the 0.2ml(flat) test tube, you just need to take on the block assembly with
the 0.2ml groove( see details in 3.4 substitutable block assembly), then revolve the knob
to the calibration of 0.2(flat). We use a rational and practical design, slam the door of
traditional idea of changing heat lid or even change the instrument.
Note: The calibration knob circles clockwise, namely from 0.5 to 384. Please don’t circle
it anti-clockwise so as not to break it.
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2.2.3.4 Substitutable block assembly
In order to do different experiments with different test tubes on the same instrument,
and to make it easier to change the block assembly, this instrument uses a design of
portable and self-lock. This personalized design enables the instrument can meet different
demands by changing the least assembly.
1 handle 2 press frame 3 block 4 Radiator
As the above figure shows, the consolidated design of the block assembly makes it
convenient to install and replace, all blocks are made of the same exterior size, the same
assemble method and the same installment method(different block assembly corresponds
to different test tubes). this kind of design assures the operator can quickly and efficiently
replace the block assembly according to their demand.
handle
Figure 1
Figure 1shows the state of replacing the block assembly. When the operator is replacing
the block, pull the holder upward, when the holder become vertical the block automatically
unlock from the holder, operator can easily take out the block assembly.
Figure 2 shows the state of installment. When operator has chosen the suitable block, he
only needs to hold the holder, put the block into the crust, and put down the holder.
The alnico will attract the block and the holder will self-lock it to the shell.
axis
handle
shell
axis
Figure 2
shell
This manual suits for next models
3
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