9
6. Internal quality control
We recommend using the Diaglobal control solutions LAC QS and GLU QS to
check the accuracy of measurements of lactate and glucose.
We recommend using the blood control HEM QS and ERY QS with target value in
the normal concentration area for checking the accuracy of measurements of
haemoglobin, haematocrit and erythrocyte counts.
We recommend using the Diaglobal control caps BIL QS to check the accuracy of
measurements of bilirubin.
We recommend using the Diaglobal control caps DIA QS to check the accuracy
and precision of the system photometer and reagent.
For all other parameters we recommend using the universal control sera from
company Roche, www.roche.de:
Precinorm U Order-No.: 10 171 735 122 (4 x 5 mL) for normal range
Precipath U Order-No.: 10 171 760 122 (4 x 5 mL) for pathological range
7. Measuring process
7.1 End point measurement
The absorbency after reaching the end point is measured.
It is measured against the reagent’s blank count.
Parameters: Haemoglobin (HB), Haemoglobin-SLS (HB SLS), Erythrocytes (ERY),
Haematocrit (HCT), Uric Acid (UA), HDL Cholesterol (HDL/CHO)
Calculation
Concentration = Absorbance x Factor
The erythrocyte and haematocrit counts are determined using stored reference
curves.
7.2 End point measurement, taking the sample’s blank count and pre-
programmed measuring time into account
After measurement of the sample blank count, the colour reaction is started in
the cuvette and the end point absorbency is measured after a preset period has
lapsed.
Parameters: Bilirubin (BIL), Bilirubin for the newborn (BIL N), and Creatinine
(CRE).
Calculation: Concentration = Absorbance x Factor
Measurement time: 2 Minutes
The samples are measured in succession:
Sample 01: Measurement 1 (sample blank value)
Sample 01: Measurement 2 (result)
Sample 02: Measurement 1 (sample blank value)
Sample 02: Measurement 2 (result)
etc.
