BioLAB BSFL-103 User manual
Operation Manual
Fluorescence Spectrophotometer
BSFL-103
Thank you for Choosing Biolab products. Please read the “Operating Instructions” and
“Warranty” before operating this unit to assure proper operation.
Index
. Preface.................................................................................................................................................03
2. Appearance and Performances of the Instrument .............................................08
3. Operation of the Instrument....................................................................................................... 4
4. Installation of the Instrument...................................................................................................... 9
5. Maintenance of the Instrument..................................................................................................24
6. Environmental Requirements of Using Software and Installation.................................26
7. Operation of Software.....................................................................................................................27
8. Operation of Wavelength Scan Mode (Connected with a Computer).........................3
9. Operation of Time Scanning Mode (When Connected with a Computer).................39
0. Operation of Quantitative Analysis Mode (When connected with a Computer)......42
. Appendix: Data Processing with Microsoft Excel 2000.......................................................50
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Fluorescence Spectrophotometer BSFL-103
Preface
The method of fluorescence analysis (fluorometry) is a modern analytical method of high PMT
level and high selectivity. It much can provide information, including an excitation spectrum,
an emission spectrum, fluorescent intensity, fluorescence life, and fluorescence polarization.
The linear range of working curve is wide and hence it has been an important analytical
method in the field of trace analysis.
The BSFL- 03 Fluorospectrophotometer is a fluorophotometer using filters with a
monochromator. It has three functions, namely, wavelength scan, time scan for fluorescent
intensity, and quantitative analysis. The instrument is capable of performing two modes of
measurement: measuring with a single instrument only, and measuring with an on-line
computer. The amounts of the functions and the convenience of measuring vary with the
different modes of measurement.
For proper use of the BSFL- 03 Fluorospectrophotometer, the user of the instrument should
have a basic knowledge of using optical instruments and a basic knowledge of molecular
fluorescence spectroscopy. The user whose instrument is equipped with a computer also
should have a corresponding knowledge and skills of operating the computer.
Before installation or the first use of this instrument, the user must read through this
instruction manual carefully. According to the user’s different equipment, the
spectrophotometer main unit may be equipped with an external computer or a special printer.
Our company guarantees that if the instrument is subject to damage or fails to work normally
due to the imperfect manufacture of our product, the company will be responsible for the
repair of the instrument (excluding quick-wear parts and consumables) without charge within
the warranty period of 2 months from the delivery date under the condition that the user
complies with the rules of transport, maintenance and operation. If the instrument is in need
of repair, please send it and its accessories back to our company, along with the quality
assurance card of our product.
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Fluorescence Spectrophotometer BSFL-103
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Alert Symbols of Safe Operation
!
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Fluorescence Spectrophotometer BSFL-103
Symbol indicates important information of operating the instrument
Please read carefully and operate according to the instructions
following.
This symbol indicates a potentially hazard to the electrical appliances
through the operation, and indicates that a qualified professional man is
needed to deal with such a hazard according to the corresponding
procedures. (This label is attached on the power switch and trigger.)
This symbol indicates heating on the surface. Please
perform cautiously according to the instructions following.
Points for Attention to Operation
. The BSFL- 03 Fluorospectrophotometer is suitably used in a laboratory room to perform
analytical measurements. If the instrument needs to be used at a worksite, the working
environment of the worksite should accord with the demands of the laboratory environment
basically.
2. If the user needs to move the instrument to other working site for analytical measurements,
please use original packing materials on moving.
3. Be sure to turn on∕off the power of the instrument according to the operating procedure.
When starting up the instrument, please turn on the power source of the xenon lamp first.
Then turn on the power source of the spectrophotometer main unit after the xenon lamp is lit.
When shutting down the instrument, please turn off the power source of the
spectrophotometer first and then the power source of the xenon lamp. Otherwise, the
instrument may not work normally. After the xenon lamp is lit, it is necessary to wait for 30
minutes over till the lamplight becomes stable. After the power source of the xenon lamp is
turned off, please wait for 60 seconds, and then turn on the lamp if the lamp needs to be used
again.
4. When the power source of the xenon lamp is turned on but the lamp fails to be lit and a
squeaking sound of high frequency or a cracking sound of sparking is produced continuously,
please turn off the power source of xenon lamp immediately and wait for several seconds,
then turn on the lamp again. Because the life of the xenon lamp is closely related to the
number of on-off of the lamp power source, the lamp should avoid being turned on/off
repeatedly under a high voltage and the unnecessary number of on/off of the xenon lamp
power source should be cut down as far as possible.
5. When the instrument is working, the surface temperature of the heat dissipation opening at
the top of the left back side of the instrument (xenon lamp position) becomes very high. Be
sure not to touch the surface of the heat dissipation opening with hands in order not to be
burnt. The air around the heat dissipation opening should be kept circulating.
6. After each startup of the instrument, please first confirm that the heat dissipation fan at the
side of the instrument runs normally in order to ensure normal work of the instrument. If the
user finds anything wrong with the heat dissipation fan, the instrument should be closed
down for an overhaul.
7. In order to protect the photomultiplier when PMT level is higher (>6), do not let high light
Fluorescence Spectrophotometer BSFL-103
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pass into the sample compartment. When a sample of unknown concentration is measured,
PMT level should be set from low grade to high grade ( - 7) gradually.
8. After PMT level is adjusted, the original set for zero-point of fluorescence should be
checked and note that zero-point adjustment may be needed again.
9. When and only when a computer error is due to the operator¹s false operation or other
interferences, the power switch of the spectrophotometer main unit should be turned off
immediately. When the software cannot be operated, please use task manager to end the
“BSFL- 03pc.exe” process, then restart the software and the instrument.
0. Within the monochromator the tightened screws must not be loosened. The optical
devices and the working environment of the instrument should be kept clean.
. When opening the cover to inspect the instrument, be sure to turn off the power source.
Particularly after the upper cover of the instrument is removed, as there are electrical
appliance parts with high voltage located at the left back of the instrument base, be sure not
to contact these parts while the power source is still turned on.
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Fluorescence Spectrophotometer BSFL-103
0 Appearance and Performances of the
Instrument
. Appearance of the Complete Instrument and Main Components
. 38% The Complete InstrumentThe Complete Instrument
Fig. - BSFL- 03 Fluorospectrophotometer Main Unit
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Fluorescence Spectrophotometer BSFL-103
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Heat Dissipation Opening at the Top of the Instrument
Heat Dissipation Fan
Lid of Sample
Compartment
Power Line
Operating Panel
USB Interfa e
RS232 Communi ation Conne tor
Fig. -2 The Left Side View of BSFL- 03 Fluorospectrophotometer Main Unit
Sample Compartment (inside it a cell holder used to hold a sample cell), Power Switch (used
to turn on/off the power source of the instrument), Fuse Holder (used to mount a fuse),
Power Socket (used to interconnect a feed cable), Xenon Lamp Switch (used to turn on/off
the Xenon lamp).
Main Components
1. Xenon Lamp
Fig. -3 Xenon Lamp and Lamp Holder
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Fluorescence Spectrophotometer BSFL-103
Line
Xenon Lamp (The
lightening part)
Lens
Line
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