Apera Instruments DO850 User manual

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DO850

Portable Optical Dissolved Oxygen Meter

Instruction Manual

APERA INSTRUMENTS, LLC

www.aperainst.com

v2.1

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Table of Contents

1. Overview --------------------------------------------------------------------------------------------------

2. Technical Specifications -------------------------------------------------------------------------------

3. Instructions -----------------------------------------------------------------------------------------------

3.1 LCD Screen -----------------------------------------------------------------------------------------

3.2 Key Operation --------------------------------------------------------------------------------------

3.3 Batteries ---------------------------------------------------------------------------------------------

3.4 Instrument Socket ---------------------------------------------------------------------------------

3.5 Reading Stability Mode ----------------------------------------------------------------------------

3.6 Auto Lock Mode -------------------------------------------------------------------------------------

3.7 Backlighting ------------------------------------------------------------------------------------------

3.8 Automatic Power-Off ------------------------------------------------------------------------------

4. Optical Dissolved Oxygen Probe -------------------------------------------------------------------

4.1 Probe Structure -------------------------------------------------------------------------------------

4.2 Probe Maintenance ----------------------------------------------------------------------------------

4.3 Sensor Cap ------------------------------------------------------------------------------------------

5. Preparation for Calibration ---------------------------------------------------------------------------

5.1 Dissolved Oxygen Units Selection ------------------------------------------------------------

5.2 Resolution Selection ------------------------------------------------------------------------------

5.3 Temperature Unit Selection ---------------------------------------------------------------------

5.4 Air Pressure Compensation ----------------------------------------------------------------------

5.5 Salinity Compensation-------------------------------------------------------------------------------

6. Calibration ------------------------------------------------------------------------------------------------

6.1 Saturated Oxygen Calibration --------------------------------------------------------------------

6.2 Zero -Oxygen Calibration --------------------------------------------------------------------------

6.3 Special Notes for Calibration ---------------------------------------------------------------------

7. Measurement ----------------------------------------------------------------------------------------------

8. Parameter Settings ---------------------------------------------------------------------------------------

9. Complete Kit -----------------------------------------------------------------------------------------------

10. Warranty ----------------------------------------------------------------------------------------------------

11. Trouble Shooting -----------------------------------------------------------------------------------------

Appendix A: Oxygen Solubility Table (760mm Hg)----------------------------------------------- !

Appendix B: DO% Calibration Values --------------------------------------------------------------

10!

11!

13!

14!

15!

16!

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1 Overview

Thank you for purchasing Apera Instruments DO850 Portable Optical Dissolved Oxygen

Meter. The DO850 measures dissolved oxygen in water using luminescence technology

through an optical sensor and displays data using advanced intelligent instrument.

Compared to electrochemical oxygen analyzers, the DO850 is more accurate and stable,

easier to use and cost-effective portable dissolved oxygen meter.

Before you use the instrument, please carefully read the instruction manual to help you

properly use and maintain.

1.1 Luminescent optical sensor.

l Stability and Accuracy: Oxygen is not consumed during measurements. It is not

affected by sample flow rate and thus provides a stable measurement.

l Easy to Use: No electrolytes and membranes are present in the meter; no warm-up;

frequent calibration is not necessary.

l Interference-Free: Sensor cap is coated with a light-shielding layer and minimizes the

impact from external light sources. The use of non-chemical sensors helps reduce a

variety of heavy metal ions interference in the aqueous environment, along with H2S

and NH4 and other chemical substances.

l Long service life. Other than mechanical deterioration (such as scratches to the light

shielding layer), the sensor cap has up to 8000 hours of service life.

l Easy to Calibrate and Maintain. Probe is equipped with calibration/storage sleeve,

which makes calibration and maintenance more convenient and reliable.

1.2 Advanced Intelligent Instrument

l Automatic temperature compensation, automatic pressure compensation and manual

salinity compensation.

l Clear large-size LCD display with white backlight.

l Reading stable and automatic locking modes.

l Meets IP57 waterproof rating; in addition, a standard instrument suitcase is provided.

Special Notes

lSensor cap surface coating can not withstand high temperatures, so the

optical dissolved oxygen electrode can not test the water of more than 50 ℃.

lSensor cap must be kept in a humid environment, if the surface coating is

dry, treat it by hydration, otherwise the measured value is unstable or the

response is slow, as described in section 4.2 (Probe Maintenance)

lAfter meter power on, wait about 30 seconds to read value or operatation.

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2 Technical Specifications

Dissolved

Oxygen

Dynamic Range

(0-20.00) mg/L (ppm), (0-200.0)%

Resolution

0.01/0.1mg/L (ppm), 0.1/1%

Accuracy

±2% reading or ±2% saturation,

whichever is greater

±2% reading or ±0.2 mg/L,

whichever is greater

Response Time

≤30 s (25℃ , 90% response)

Calibration Points

Saturation Point & Zero Oxygen

Tem pera tu re Co mpen sa tion

Automatic, (0 to 50)℃

Pressure Compensation

Automatic, (60 to 120) kPa

Salinity Compensation

Manual, (0 to 45) ppt

Temperature

Range

(0 to 50.0) ℃

Resolution

0.1 ℃

Accuracy

±0.5 ℃

Other

Batteries

AA x 3 (1.5V×3)

IP Rating

IP57

Dimensions and Weight

Meter: 88×170×33 mm/313g

With case: 360×270×76 mm/1.3kg

Product Certificate

RoHs, CE & ISO9001:2008

3 Instructions

3.1 LCD Screen

Fig.-1

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3.2 Key Operation

Short press: key press time < 2 s; Long press: key press time> 2 s.

Power on: Press to turn on. Shutdown: long press 2 seconds off.

Special notes: After meter power on, wait about 30 seconds to read value or operatation.

Tabl e - 1 Key operation and functions

Key

Operation

Functions

Short

Press

l In the shutdown mode: press the key to boot

l In measurement mode: press to turn backlight on or off

Long Press

l Press and hold for 2 seconds to turn off

Short

Press

l In measurement mode: press the key to switch unit: %→mg/L

or %→ppm

Long Press

l In the measurement mode: press the key for 2 seconds to enter the

calibration mode

Short

Press

l To cancel any operation, press to return to measurement mode

Short

Press

l In the menu mode: press the key to change the serial number or

select the parameter

Short

Press

l In the menu mode: press the key to change the serial number or

select the parameter

①!

Measurement mode icon

⑦!

Auto lock icon

②!

Reading/Measured Value

⑧!

Tem pera tu re Co mpen sa tion

ATC — Auto Temperature Compensation

MTC — Manual Temperature Compensation

③!

Symbol

④!

Units of Measurement

⑤!

Tem pera tu re un it

⑨!

Reading Stability Icon

⑥!

Tem prat ure v al ue / Symbol

⑩!

Low Voltage Icon

Fig.-2

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Short

Press

l In measurement mode: press to enter menu mode;

l In calibration mode: press key to calibrate;

l In the menu mode: press key to confirm the parameter.

3.3 Batteries

The instrument uses three AA batteries, please use the LR6-type

alkaline batteries to ensure battery quality. Battery life> 200 hours (no

backlight). When the display shows symbol as Figure-3, replace

the battery.

3.4 Instrument Socket

The instrument uses an 8-pin socket that is protected by a gray rubber cap seal. When

inserting the Probe plug, please insert it after the notch position, and tighten the plug nut.

The end face of the socket and the plug connection has the sealing ring, which can

effectively maintain the waterproof protection of the socket.

3.5 Reading Stability Mode

When the measured value is stable, the LCD screen displays the icon

as shown in Figure-4. If there is no icon or icon flashing,

indicating that the measured value is not stable, the measured value

should not be read or calibrated.

3.6 Auto Lock Mode

In parameter setting P4.2 you can select the auto-lock mode (Off-On),

select On to turn on automatic locking. When the reading is

stable for more than 10 seconds, the meter automatically locks the

measured value and displays the HOLD icon, as shown in Figure 5.

When auto locked, press to unlock.

3.7 Backlighting

The Instruments LCD screen has a white backlight suitable for use in dimly lit environments.

Turning on the backlight will consume more power. The instrument is equpped with

automatic backlight and manual backlight mode. In automatic backlight mode, when

thepower key is pressed, backlight will be on for a minute then automatically shuts off;

In manual backlight mode, when the key is pressed, backlight will be on and will be off

only is pressed again. In the parameter setting P4.3, you can select the auto backlight

mode (On-Off), select On to turn on auto backlight, select Off to turn off auto backlight.

3.8 Automatic Power-Off

In the parameter setting P4.4, you can select the auto power off function (On-Off), select On

to turn on auto power off, the instrument will shut down automatically after 20 minutes, select

Off to disable this function.

Fig.-3

Fig.-4

Fig.-5

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4 Optical Dissolved Oxygen Probe

4.1 Probe Structure

The instruments DO803 optical dissolved oxygen probe has a cable length of 3m and built-

in temperature sensor for automatic temperature compensation. The electrode structure is

shown in Fig.-6

1. Bottom cover of the calibration sleeve

5. Locking cap

2. Sponge for water storage

6. Optical DO electrode

3. Sensor cap

7. O ring

4. Calibration sleeve

Immersion line:

The tested solution should be above this line

4.2 Probe Maintenance

The sensor cap of the optical DO electrode must be kept in a moist environment. If the

surface coating of the sensor cap dries out, the measured value will be unstable or the

response will be slow. The electrode calibration sleeve is used to store the probe.

(A) Short-term storage (less than 30 days): The probe head is kept in the calibration

sleeve. Always keep the sponge inside the calibration sleeve wet. Several drops of

clean water should be added to a dry sponge (let the sponge be saturated, but not

dripping), and tighten the lock cap, so that the sensor cap is kept in the moist-

saturated air.

(B) Long-term storage (greater than 30 days): The probe head is kept in the calibration

sleeve. Check whether the water storage sponge is moist every 30 days or user can

store the electrode in a beaker containing water.

sponge is dry or if the electrode is exposed to dry air for more than 8 hours, the

surface coating of the sensor cap may be completely dry. So the electrode should be

Fig.-6

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immersed in tap water at room temperature 25℃ for 24 hours. If the water temperature

is low, soaking time is 48-72 hours.

(D) The sponge can not be allowed to get stained or moldy, otherwise it will consume or

produce oxygen. If stained or moldy, please clean immediately.

4.3 Sensor Cap

(A) The sensor cap is an important part of the optical DO probe. The surface coating of

the cap cannot be scratched or mechanically worn. Otherwise, the life of the sensor

cap will be reduced or the probe will be damaged. Please pay special attention to it

when using the probe.

(B) The surface coating of the sensor cap cannot withstand high temperatures, so the

optical DO probe can not be tested in water above 50 ℃.

organic solvents to clean, otherwise it may damage the probe. It can be gently wiped

with a soft cloth or paper towels. To disinfect the probe, immerse it in 3% hydrogen

peroxide for 15 to 30 minutes and then rinse off with water.

(D) The sensor cap has a service life of more than 8000 hours. When the probe is not

being used, it does not "bleach" luminescence layer; in addition, the storage time will

not reduce the life of the probe, so the actual use time of the sensor cap is far more

than a year. The major factor affecting the service life of the sensor cap is the surface

coating being damaged under external force. So the key is to protect the sensor cap

from external damage.

(E) If the sensor cap is damaged or deteriorated, users need to purchase a new one.

Every new cap has a set of calibration codes which need to be input into the

instrument. The specific input method will be described in the instruction manual of

sensor cap.

(F) The probe that comes with the instrument can be used directly without the input of the

calibration codes. So users should not take off the sensor cap when it is not in use.

Nor should one swap the caps from different instruments. When being installed, the

sensor cap must be tightened and the interior can not be contaminated or wet.

5 Preparation for Calibration

5.1 Dissolved Oxygen Units Selection

Dissolved oxygen units displays in two forms: mg / L and %, and ppm and %. Press to

switch between mg / L → %, or ppm → %. Users can choose mg / L or ppm in parameter

setting P3.1, but only a percentage (DO %) is displayed in calibration.

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5.2 Resolution Selection

The resolution unit can be selected in parameter setting P3.2: 0.01 or 0.1mg/L (ppm). After

setting, the meter will display resolution of 0.1 or 1 in according to %.

5.3 Temperature Unit Selection

The temperature unit can be selected in parameter setting P4.1: ℃ or ℉.

5.4 Air Pressure Compensation

The instrument has automatic air pressure compensation function. The instrument has been

performed the air pressure calibration before it leaves the factory. So generally users do not

have to calibrate the air pressure. If necessary, calibrate it according to the value of the

standard barometer. Refer to parameter setting P3.4 for the procedure of barometric

calibration.

5.5 Salinity Compensation

The instrument has manual salinity compensation. It is set in the parameter P3.3 (0 to 45

ppt). To obtain accurate readings in mg/L and ppm, it is necessary to know the salinity of the

solution to be tested and input it into the instrument. As the salinity of the solution increases,

the level of DO decreases. Generally, salinity of freshwater is 0 to 0.5ppt, salinity of seawater

is 35ppt.

6 Calibration

6.1 Saturated Oxygen Calibration

(A) This procedure requires the use of a calibration sleeve to allow the probe to be calibrated

in a humidity-saturated atmosphere

(B) Check that the sponge in the calibration sleeve is wet. Attach the calibration sleeve to

the probe. Tighten the locking cap. Be careful not to have water droplets on the head of

the cap. Wait for 5 to 10 minutes after turning on the instrument. Saturate the air in the

calibration sleeve with water vapor. In addition, wait for the temperature to completely

stablize.

upper right corner of the LCD. Wait for the stable icon to appear and stay, press the

key to calibrate, once the instrument displays a stable 100%, it is ready to use.

6.2 Zero -Oxygen Calibration

Zero-Oxygen calibration is only performed when a probe or sensor cap is replaced or the

probe has not been used for a long period. It is usually not necessary to conduct zero oxygen

calibration. The instrument is factory calibrated for zero oxygen, so it is not necessary to

perform a zero-oxygen calibration for the first time. Zero-oxygen calibration should follow

these steps:

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(A) Preparation of 100ml of oxygen-free water: in the 100ml beaker weigh 2g anhydrous

sodium sulfite (Na2SO3) and add 100ml of pure water or tap water to dissolve.

Oxygen-free water is effective within 1 hour.

(B) Put the electrode into the oxygen-free water, wait for 3 to 5 minutes after the

instrument is turned on, and wait for the temperature and measured value to

completely stabilize. The measured value should be very low, 0.1mg / L or so.

flash CAL. Wait for a stable icon. Press and the zero-oxygen calibration is

completed.

6.3 Special Notes for Calibration

(A) Optical dissolved oxygen probes have better stability and smaller calibration drift

compared to conventional electrochemical dissolved oxygen electrodes, which means

that the instrument can maintain its calibration data for a few months. However, for better

accuracy, it is recommended that the oxygen saturation calibration be performed

according to section 6.1 before use every day.

(B) Drying of the surface coating of the sensor cap can adversely affect the stability of the

measurement. Please pay special attention to this situation. See Section 4.2 (Probe

Maintenance) for details.

the meter will be calibrated to the theory value.

7 Measurement

7.1 When measuring, place the probe in sample solution, swirl quickly in the solution for a

few seconds and rest it to remove bubbles from the measuring surface of the sensor cap.

The solution must be above the immersion line of the probe. Note that brief shaking the

probe in solution is only to eliminate bubbles. Not like conventional electrochemical

electrodes, the measuring via optical dissolved oxygen probes does not require constant

stirring of the solution or flowing fluid.

7.2 Users can read the measured values when the icon appears and stays. Note that

the measurement time is related to temperature. When the solution temperature and the

probe temperature is close, the measurement time is about one minute. When the solution

temperature and the electrode temperature differ a lot, it takes about 3 minutes to reach a

stable reading. This is because the reading of dissolved oxygen is heavily influenced by

temperature, and the probe senses temperature much more slowly than dissolved oxygen.

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