Olympus Fsx100 User manual

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Introduction

SAFETY PRECAUTIONS

Observation Methods

Introduction

Fluorescence

observation

This method utilizes the

fluorescence generated

when the specimen is

irradiated with strong light

to observe the specimen

structure or identify the

substance according to the

presence/absence and color

tone of the ﬂ uorescence.

Phase contrast

observation

T h i s m e t h o d m a k e s i t

possible to observe non-

stained specimen (living

specimen) or nearly

transparent specimen by

enhancing the contrast

between brightness and

darkness.

Bright Field

observation

This is the most general

method for use in obser-

vation of stained specimens

and contrasted specimens.

Observation/Acquisition Flow Main Controls

During observation and acquisition, the cover is

kept closed to avoid the risk on the user. However,

when placing or replacement, the cover should be

opened so care is required in these operations.

Caution against

high temperature

This section accommodates

the light source bulb and may

become hot.

Caution against

ﬁ nger being caught

Do not approach your hand or

ﬁ nger to the motorized stage

and motorized revolving

nosepiece.

Caution against

direct view

This section outputs bright

light during ﬂ uorescence

observation. Be careful not to

stare it directly.

Main Controls

Introduction Observation/Acquisition Flow

Main Controls

Controrls

Main switch

： ON

： OFF

Close lever

Push down to close the

cover.

Open button

Push down to open the

cover.

STATUS LED

Lights in green: Sub-switch ON

Blinks in green: Sub-switch OFF.

Sub switch

To turn OFF, keep it depressed for

5 seconds.

FL BURNER LED

Lights blue when the ﬂ uorescence

observation light is ON.

Cover

The controls other than the

above can be adjusted and

set from the software.

(The operations are easy

thanks to the operation guide

display.)

Do not set the main switch to OFF for at

least 3 minutes after setting

the sub-switch to OFF to allow the bulb to

cool down.

Observation/Acquisition Flow

IntroductionMain Controls

Observation/Acquisition Flow

Starting the

System

p. 6

Placing the

Specimen

p. 7

Selecting the

Observation/

Acquisition Methods

p. 8

This section describes the basic

operation ﬂ ow in sequence together

with the monitor screen displays.

Observation/Acquisition Flow

Main ControlsIntroduction

When using the unit for the ﬁ rst time:

Activation is required.

(For the Activation procedure, refer to

the Instruction Manual [Hardware].

The description in this section assumes

that the following preparation work has

been completed.

・ The cables are connected properly.

・ The software has been installed.

・ The lamp is attached.

(For the operating procedures for the

above, refer to the Instruction Manual

[Hardware].

CAUTION

Observation/

Acquisition

Procedure

p. 9

Operation Guide

Display

p. 10

Expanding

the Scope of

Acquisition

p. 11

Observation/Acquisition Flow

Main Controls Introduction

Double-click on the FSX-BSW icon on

the desktop to launch the software.

1. Starting the System

Observation/Acquisition Flow

Set the main switch to “ l ” (ON).

Push the sub switch to turn it on.

Set the main switches of the computer

and monitor to ON.

Log on Windows Vista with your own

user ID.

The splash screen appears on the

monitor screen.

The image data acquired with this unit

and the acquisition settings are saved

on a per-user ID basis.

Now the system has started up.

2. Placing the Specimen

The specimen can be placed in position

when the instruction to place the

specimen is displayed.

Push down the

open button 2,

open the cover and

place the specimen.

Push down the close lever and close

the cover.

Click on the button

and open the Acquisition menu.

Push in the lock lever toward the left

and hold it in the pushed-in position.

Pull out the moving lever toward you

(all the way until it stops).

Pull out the slide glass holder toward

the right to set the slide glass in

position.

Push down the close lever and close

the cover.

Click on the button

and open the Acquisition menu.

Push in the lock lever toward the left

and hold it in the pushed-in position.

Push in the moving lever toward the

deep (all the way until it stops).

Observation/Acquisition Flow

Main ControlsIntroduction

Place the slide glass.

Be sure to place it with the cover glass

facing down.

Slide glass specimen

Now the specimen has been placed in position.

Place the dish by applying against the

dish stopper 5.

Φ 35 mm dish specimen

Observation/Acquisition Flow

Main Controls Introduction

3. Selecting the Observation/Acquisition Methods

Observation/Acquisition Flow

Click on the buttons enclosed in yellow frames.

Select the

observation method.

Fluorescence & Phase

contrast observation /

Phase contrast

observation /

Bright Field observation

Select the acquisition

method.

Single /

Time-Lapse /

Z-Stack /

Stitching

(Scheduled to be available

in March 2009)

Select the ﬂ uorescence color to be

used as the observation standard

or the phase contrast.

Green / Red / Blue / Phase contrast

Start button The selection

summary is

displayed.

The subsequent description in this manual assumes that the following

selections are made.

Observation method: Fluorescence & Phase contrast observation

Observation standard color: Green

Acquisition method: Single

Now the observation and acquisition methods have been selected.

4. Observation/Acquisition Procedure

Adjust the focus and magniﬁ cation.

Optimize the image.

Fluorescence only Overlap the

ﬂ uorescence color or phase contrast

images and conﬁ rm the acquisition

condition.

Click on to acquire

the image.

Observation/Acquisition Flow

Main ControlsIntroduction

Locate the observation target ﬁ rst.

Move the observation position

(with drag & drop) to locate the

observation target.

Click on to go

to the next screen.

See next page for the operation guide display.

Tightly target at the observation

area in the macro image.

:Move the target frame.

：Enlarge or reduce

the target frame.

Click on to go

to the next screen.

Now the acquisition is complete.

Adjust the image and acquire it.

Observation/Acquisition Flow

Main Controls Introduction

5. Operation Guide Display

Observation/Acquisition Flow

The operation

guide shows the

basic procedure

for completing

acquisition with

the selected

observation/

acquisition methods.

Click to view the description

text.

Click to view the one-point

tip.

Click to go to the next screen.

Description text.

The Online Help gives more detailed explanations.

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