Olympus SLIDEVIEW VS200 User manual

Instructions for use

SLIDEVIEW

VS200

Optical Microscope and Accessory

Any copyrights relating to this manual shall belong to

OlympusSoftImagingSolutionsGmbH.

We at Olympus Soft Imaging Solutions GmbH have tried to make the information contained

in this manual as accurate and reliable as possible. Nevertheless, Olympus Soft Imaging

Solutions GmbH disclaims any warranty of any kind, whether expressed or implied, as to any

matter whatsoever relating to this manual, including without limitation the merchantability

or fitness for any particular purpose. Olympus Soft Imaging Solutions GmbH will from time to

time revise the software described in this manual and reserves the right to make such

changes without obligation to notify the purchaser. In no event shall Olympus Soft Imaging

Solutions GmbH be liable for any indirect, special, incidental, or consequential damages

arising out of purchase or use of this manual or the information contained herein.

No part of this document may be reproduced or transmitted in any form or by any means,

electronic or mechanical for any purpose, without the prior written permission of

OlympusSoftImagingSolutionsGmbH.

All brands are Trademark or registered Trademark of their respective owners.

Version 510_InstructionsForUse_VS200_de_00_xxJuly2019

Original instructions

Manufactured by

OLYMPUS SOFT IMAGING SOLUTIONS GmbH

Johann-Krane-Weg 39

48149 Münster, Germany

Phone: +49 251 - 798 00 0

Fax: +49 251 - 798 00 6060

Email: info@olympus-sis.com

Contents

1 About this manual 7

1.1 Notes on qualifications 7

1.2 Intended use 7

1.3 Other applicable documents 8

1.4 Symbols in this documentation 8

2 Safety 9

2.1 Categories and symbols in the safety instructions 9

2.2 Caution labels 9

2.3 Caution labels on the product 9

2.4 General safety instructions 10

2.5 Safety instructions for the laser 13

2.6 Safety instructions for X-Cite Turbo and X-Cite Xylis 13

3 Hinweise zum Aufstellen 14

3.1 Betriebs- und Lagerbedingungen 14

4 Tools and accessories 16

5 Scope of supply 17

6 System diagram 20

6.1 Available fluorescence components 20

7 Trays 22

7.1 Tray description 22

7.2 VS200 Tray types 22

8 Specifications 24

9 Unboxing 26

9.1 VS200 Scanner 26

9.1.1 Mounting of handles - VS200 scanner 26

9.2 Transportation locks for the VS200 scanner 26

9.2.1 Removing transportation lock from stage 27

9.2.2 Removing transportation lock from transmitted light condenser

(BX3-UCD8A) 28

9.3 VS200 Loader 30

9.3.1 VS200 Trays 32

9.3.2 PC and monitor 32

9.4 VS200 loader transportation locks 32

9.4.1 Removing transportation lock from VS200 loader’s tray hotel 33

9.4.2 Removing transportation lock from SCARA robot arm 34

- 3 - Instructions for useOLYMPUS VS200 System

9.4.3 Removing transportation lock from SCARA robot arm’s coun-

terweight 35

10 Mounting of components 37

10.1 Scanner 37

10.1.1 Objectives 37

10.1.2 Immersion Objectives 38

10.1.3 Phase Contrast (PH) objectives 39

11 Mounting fluorescence components 40

11.1 IX3-RFACA and IX3-RFALFE 40

11.2 Fluorescence filter wheels or camera adapter for monochrome camera 45

11.2.1 U-FFWR (Motorized fast reflected light filter wheel) 45

11.2.2 Add or replace filter (U-FFWR) 47

11.2.3 U-FFWO T3 (Motorized fast observation filter wheel) 49

11.2.4 Add or replace filter (U-FFWO) 51

11.2.5 TV 1.0x adapter 52

11.3 Monochrome camera 52

11.3.1 Orientation of the monochrome camera 53

11.4 X-Cite adapter 56

11.5 4x Objective 57

11.6 LED light source 57

11.7 Filter set 58

11.7.1 U-FF filter cube (IX3) 58

11.7.2 Add or replace filter cube (IX3) 59

11.7.3 U-FDICT filter cube 60

11.7.4 X-Cite Turbo 61

12 Assembly of the housing for scanner 62

12.1 Left panel 62

12.2 Right Panel 64

12.3 Back panel 65

12.4 Top panel 66

13 Cabling 70

14 Assembly of the loader housing 72

14.1 Top panel 72

14.2 Back panel 72

14.3 Front panels 73

15 Verbindung VS200 Scanner und VS200 Loader 75

15.1 Mechanical Connection VS200 scanner and VS200 loader 75

- 4 - Instructions for useOLYMPUS VS200 System

16 PC operating system language 77

17 VS200 ASW software setup 78

18 VS200 device configuration 85

18.1 Activate the motorized polarizer 86

18.2 Activate the VS200 liquid dispenser 86

18.3 Device settings - objectives 87

18.4 Device settings - filter 88

18.5 Manual device configuration 89

18.5.1 ORCA monochrome camera 90

18.6 Device customization 90

18.6.1 ORCA camera adjustments 91

18.6.2 Setup phase contrast (PH) observation method 92

18.6.3 Setup polarization (Pol) observation method 94

18.6.4 Create or adjust an observation method 95

18.7 VS200 LED lamp voltages 97

18.7.1 Orca Flash 4.0 voltages (%) 97

18.7.2 Orca Fusion voltages (%) 97

18.7.3 VS-304M voltages (%) 98

18.7.4 VS-264C voltages (%) 98

19 How to insert a slide into a tray 99

19.1 How to insert a tray into the VS200 scanner 99

19.2 Insert a tray into the VS200 loader 100

20 Calibrate VS200 using the Olympus Calibration Slide 101

20.1 Stage limits - Z Axis 103

20.2 Camera adapter calibration for iDS (VS-264C) camera 106

20.3 Camera-To-Stage Rotation 110

20.4 XY Objective Shift / Parfocality 112

20.5 Shading Correction (Brightfield) 115

20.6 Shading correction of the label area 123

20.6.1 Preparation of calibration slide 123

20.6.2 Shading correction for polarization (Pol) 131

20.7 Magnification Test Scan 136

21 Additional calibrations for a fluorescence system 138

21.1 Camera adapter 139

21.1.1 Camera adapter U-FFWO T3 139

21.2 Camera-To-Stage Rotation 144

21.3 Camera shift 146

- 5 - Instructions for useOLYMPUS VS200 System

21.4 Lens Correction 150

21.5 Shading correction BFMono 153

21.6 Magnification Test Scan 158

21.7 Shading correction for fluorescence observation methods 160

21.8 Shading correction for darkfield (DFMono) 164

21.9 Shading correction for PhaseContrast (PH) 167

21.10 Channel-XY-Shift 171

22 Cleaning the system 174

22.1 Cleaning the scanner 174

22.2 Cleaning the loader 174

22.3 Cleaning the trays 175

22.4 Cleaning the X-Cite Turbo 175

22.5 Cleaning the immersion objective 175

23 Additional software installation 178

23.1 OlyVIA 178

23.2 VS200 ASW Desktop 178

23.3 NetImage Server SQL (NIS-SQL) and Webinterface 178

24 Troubleshooting 179

24.1 Hardware not available 179

24.2 "No camera" error 179

24.3 Tray not active 179

24.4 Image too bright 180

24.5 Setting the Koehler illumination 180

25 Proper selection of the power supply cord 186

26 Declarations of conformity and disposal 189

26.1 CE Conformity (Europe) 189

26.2 WEEE declaration (Europe) 189

26.3 RoHS Conformity (Europe) 189

26.4 FCC conformity (USA) 189

26.5 For Korea only 190

26.6 China RoHS conformity (China) 190

26.7 RFID 190

26.7.1 RFID (Korea) 190

26.7.2 RFID (USA) 190

26.7.3 RFID (Canada) 191

26.7.4 RFID (Taiwan) 191

27 Support 192

- 6 - Instructions for useOLYMPUS VS200 System

1 About this manual

1 About this manual

This manual for the VS200 system is for Olympus technicians as well as users of the

VS200 system who have been trained by Olympus. The manual contains important

information on how to operate the VS200 system safely and correctly.

In addition, this manual describes how to unbox and install the system as well as

how to install further components. These tasks must expressly only be performed by

Olympus. As a user you are not allowed to unbox or install the VS200 system by your-

self!

To ensure the safety, obtain optimum performance and to familiarize yourself fully

with the use of this product, study this manual thoroughly before installing this

product, and always keep this manual at hand when operating this product.

Retain this instruction manual in an easily accessible place near the work area for

future reference.

Installing and configuring a liquid dispenser, and upgrading to a VS200

loader are not in the scope of this document. These must only be installed

and adjusted by technical service of Olympus.

1.1 Notes on qualifications

Olympus personnel Instructions for tasks that must only be performed by Olympus

are identified with the following note:

The units described below must be assembled and adjus-

ted by Olympus. If these units are assembled or adjusted

by the customer, the operations are not ensured.

1.2 Intended use

The system is intended to be used for the acquisition of virtual slide images. The sys-

tem is intended to be used for research only.

If the VS200 system is used in a manner not specified by this manual, the safety of

the user may be at risk. In addition, the VS200 system may also be damaged. Always

use the VS200 system according to this instruction manual.

Olympus Soft Imaging Solutions GmbH takes no responsibility for damaged caused

by unintended use.

Foreseeable misuse

The system is not intended to be used for primary diagnosis.

- 7 - Instructions for useOLYMPUS VS200 System

1.3 Other applicable documents

Familiarize yourself with all of the other manuals for the components of the system.

Take special note of the safety instructions they contain.

1.4 Symbols in this documentation

Tools required for performing the steps

Preconditions for the subsequent steps

- 8 - Instructions for use

1 About this manual

OLYMPUS VS200 System

2 Safety

2 Safety

If the product is used in a manner not specified by this manual, the safety of the user

may be imperiled. In addition, the product may also be damaged. Always use the

product as outlined in this instruction manual.

2.1 Categories and symbols in the safety instructions

The safety instructions in this manual use symbols and keywords that are divided

into the following categories:

CAUTION

This warning sign and the word CAUTION indicate dangerous situations that

can lead to minor injury if ignored.

ATTENTION

The exclamation mark and the word ATTENTION indicate situations where irre-

parable damage to the product can occur if ignored.

Useful tips and important information

This symbol indicates useful notes, tips and important information.

2.2 Caution labels

Indicates a non-specific general hazard.

This warning sign indicates that there is a crush hazard.

2.3 Caution labels on the product

Caution labels have been placed at positions where special care is required when

handling and operating the system. Always pay attention to the caution labels.

CAUTION

Moving components can cause injury

Im Inneren des VS200 Systems verfahren Komponenten. Gaps open and close.

Hands and fingers can get crushed.

Sobald das System mit der Stromversorgung verbunden ist, Haare, Finger,

Hände und andere Körperteile sowie Kleidung und Gegenstände aus dem

Inneren des VS200 Systems fernhalten.

- 9 - Instructions for useOLYMPUS VS200 System

Position of caution labels

U-FFWO

Loader

Im Bereich des Mikroskoptisches

Nosepiece

Flap door

Flap door motor

2.4 General safety instructions

Do not plug anything into or unplug anything from the ports when the system is

connected to the power supply.

The system is not intended to be used for general microscopy tasks.

No guarantee is provided on an image file. For example, even if the following

state is generated attributable to an operation error of an observer, malfunction

of the device, a system failure including a potential one, or the like, or at the time

of normal operation or normal movement, it will not be possible to perform

image restoration or damage compensation.

1. Abnormal state of an image

2. Abnormal state of file property such as a file name and file date and time

3. Disappearance of an image

Confirm, edit or save the image for backup through care of the observer. To be

noted is that the electronic recording media such as USB memory, HDD and

DVD-R have retention lives in general and there may be the case that a file saved

may disappear in several years.

Further, there is no authenticity or tamper-proof characteristic.

Prevent from strong vibrations as they can impair the image quality.

Regarding to 3-axes control unit for scanning stages and motorised focus drives,

1. Item caution for use

2. Installation prevent from strong vibrations and risk of explosion

3. Temperature prevent from direct sunlight or strong heat sources

4. Humidity prevent from splash water and moisture

- 10 - Instructions for use

2 Safety

OLYMPUS VS200 System

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