Olympus AH3 Operating instructions

RESEARCHPHOTOMICROGRAPHICMICROSCOPESYSTEM

MODEL:AH3

REPAIRMANUAL

INDEX

Outline of product . . . . . . . ..C................ Gray

Repair procedure . . . . . . . . . ..*................ Green

Trouble shooting . . . . . . . . . . . . . . ..*........... Yellow

Jigs, Tools and Chemicals list . . . . . . . . . . . . . . Orange

Appendix . . . . . ..*............................ Blue

E-l A2KCOlll Instruction Manual

E-2 BUILT-IN BATTERY Replacement Manual

OLYMPUS

AH3 A. OUTLINE OF PRODUCT

CONTENTS

1. OUTLINE OF MERCHANDISE..................................... A-2

AHBS3 ..; ................................................... A-2

AHBT3 ...................................................... A-2

AHMT3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..-...... A-2

2. FEATURES ................................................... A-2

AHBS3 ...................................................... A-2

AHBT3 ........................................................ A-3

AHMT3 ...................................................... A-3

3. USING CONDITIONS ........................................... A-4

AHBS3 ................................................ ...... A-4

AHBT3 ...................................................... A-5

AHMT3 ...................................................... A-6

4. COMPATIBILITY .............................................. A-8

Compatibility between AH2 and AH3 units .................... A-8

Table of compatibility between AH2 and AH3 (other

microscope) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ..--...... A-9

Table of AH3 revolution unit used sliders .*........-....... A-9

5. SPECIFICATIONS ............................................. A-10

AHBS3 ...................................................... A-10

AHBT3 ...................................................... A-12

AHMT3 ...................................................... A-13

6. IDENTIFICATION OF VARIOUS COMPONENTS

..*.............-......- A-16

A-l

AH3 A. OUTLINE OF PRODUCT

1, OUTLINE OF MERCHANDISE

AHBS3

This is the automatic type PHOTOMICROGRAPHICAH3 body

for

biology

designed for use in laboratories, which is remodeled from AH2 (AHBS)

by widening the auto focus range.

AHBT3

This is the manual type PHOTOMICROGRAPHICAH3 body for biology

designed for use in laboratories, which is remodeled from AH2 (AHBT)

by making the CONDENSERTURRET automatically interlocking with

the objective magnification.

AHMT3

This is the UPRIGHT TYPE PHOTOMICROGRAPHICAH3 body for metallurgy

designed for use in laboratories, which is remodeled from AH2 (AHMT)

by increasing the stage vertical mountable range 18mm to permit

observation of a thick specimen.

2, FEATURES

AHBS3

(1) Built-in photographing unit of the AVERAGE/SPOTMETERING

selectable type

(2)

Attachable with three cameras and one TV camera at same time

(3) Auto focusing from low to middle magnification (objective 1X - 40X)

(Auto focus features called AF)

(4j

Scale and data imprintable and photographing in half size

(5) Single lens REFLEX FINDER

(6) Motorized REVOLVINGNOSEPIECE

(7) Illumination systems such as condenser, field diaphragm, aperture

diaphragm are all adjusted automatically, linked to each objective

magnification.

(8) Full KOHLERILLUMINATION

(9) Super wide field of view (FIELD NUMBER26.5)

(10) Four built-in photographic eyepieces (2.5X, 3.3X, 4x, 5x)

(11) Built-in light balancing filter, ND filter and contrast filter

(12) 'Gu‘henthe built-in lamp switch is turned off in observation of

reflected light fluorescence (using AH3-PFCA), the condenser, AS

and ND are not moved even if an objective is changed.

(This is the feature for the user who use the reflected light

fluorescence only and does not use the transmitted light.)

(13) This model (A2-AAC-2) has improvement against

protection

of self

fluorescence light of A2-AAC in observation with the reflected

light fluorescence

A-2

AH3 A. OUTLINE OF PRODUCT

Table of compatibility between AH2 and AH3 (other

microscope)

A24RE2

A2-AAC2

A2-CHM

A%TCS2

A2-TCT2

AH3-UMA

AH3-RFCA

AHBS

*;3424

*5,7

*25,27

*26,28

AHBT

*2,24

*5,6,7

*25,27

*28

AHMT AHBS3

*i?ii

*5:6,7

*5,6,7

*27,30

*28

*2,3,24

*5,7

*25,27

ABBT3 1 AHMT3

*1 - 23 See the preceding page.

"24: Overlap with the guide dovetail decreases if attached with the

fixing screw located top.

"25: Interferes with the stage when mounting the reflected light

Nomarski prism.

*26: AF malfunction, different AS/FS initial setting, malfunction

in magnification display, A2-TCS2 works with the lamp switch OFF.

*27: Ghost is seen when viewed from the oblique direction.

*28: Too much SELF FLUORESCENCELIGHT (when using A2-AAC)

"29: Composite observation is impossible.

*30: No stage stroke increase

. Table of Sliders for each revolving nosepiece unit.

A2-6RE2 .

AH2-AN4:

AH2-KPO

AH2-NAS

(AZ-NA)

AH2-NAS

(A2-NS)

Transmitted

polarization

0 AN

0 PO

- NIC+AN

- NIC+PO

.* Combination is the same as AH2.

* A2-NS ,is for SPlan.

AN: Analyzer

PO: Polarizer

NIC: Nomarski observation.

Slider Transmitted NIC Transmitted

DPLAN SPLAN polarization

AH3-ANF 0 0 0 AN

AH3-NAF 0 0 - NIC

AH3-NSF 0 - - NIC+PO

AH2-NAS - 0 NIC+PO

(A2-NS)

AH2-KPO - - 0 PO

* AH3-NSF is for DPlan.

* AH3-ANF is used for AH3-UMA.

A-9

AH3 A. OUTLINE OF PRODUCT

4, COMPATIBILITY

- Compatibility between AH2 and AH3 units

AHBS3 AHBT3 AHMT3

A2-BTL

*1 *l *l

A2-CH *2,3 *2 *2

A2-AAC *4 *4 -

A2-TCS *5 *5 -

A2-TCT *5,6,7 *5,6,7 -

A2-AAS *8 - -

A2-AST *8 *8 -

A2-LS *lO *lO *lO

A2-FIL 0

A2-MM 8 8 0

A2-CB 0

4H2-MA *ll

4H2-RLA *ll 0 g

*12

4H2-RFL(RFA) *13 *14 *15

4H2-TFL(RFA) *13

*14 -

3H2-SIC6R(L) *l&17 *17 *17

4H2-WHR

* 16317 * 17 *17

4H2-ilH

* 16,17 *17 *17

tH2-LWCD g 0 0

\H2-RS

iH2-LSRF 0 Ei

\H2-SLM 8 g s

\H2-SLH

\H2-ADF

\H2-DL-2 O t? 00

0 0

\H2-#R(L) *18

*I8

- *18

\H2-NA *9 *9 -

\H2-NS *9 *9 -

\H2-?l-NIC *Cl,19 *I9 *19

\H2-M-NICUL x11,19 *19 *19

\H2-N-NIC

*11,20 *20 *20

\H2-AN-45 *21

*21 *22

\H2-KPO

*23 a23 -

\H2-DCD

\H2-DCW 8 s -

\H2-DCH

\H2-KDC 8 8 =

{ANDSW-1 0 0 0

0: Compatible

*: Conditioned

-: Not mountable or not operable

*1 : Fs centration of low power

objective is not ensured.

*2 : Stage squareness adjustment

required

*3 :

standard position adjust-

ment required .A

*4 : FS/AS centration is not ensured

due to inclination (adjust in

pairs with CH)

*5 : FS centration of 4X or

1eSS not

"6 : Manual operation

ensured

: A2-CS not mounted

*8 : AS centration not ensured A

*9 : -

*10: Lamp centration not ensured

*11:

Specimen may be broken when

taking correction data.

*12: Momentary malfunction during

interlocking of the revolving

nosepiece and Condenser

"13: AF impossible, parts related to

the revolving nosepiece not

operated, objective not displayed,

stage speed indefinite.

"14: Condenser not interlockedwith

objective-A

*15: Transmitted light fluorescence

is impossible

*16: Focusing part not durable

*17: Not perpendicular to optical axisA

*18: Stage insert plate is not com-

patible due to partial defocusingA

*19: Possible if AH2-MA is used A

*20: Possible if AH2-RLA is used A

*21: Use a modified part inclined 3O

-when combining with AH2-KPO in

transmitted SIMPLE POLARIZ1NG.A

*22: Reflected light Nomarski Observa-

tion is possible by attaching to

AH2-MA. A

"23: Use a modified part inclined 3O

when combining with AH2-AN45 in

transmitted simple polarizing. A

Note) "A" is the same condition

as AH2.

A-8

AH3 A. OUTLINE OF PRODUCT

Stage: SVR, SVL Unit: mm

g With Nomarski Prism Without Nomarski Prism

$ :{ Observable stage stroke Observable Stage stroke

i-3; specimen of Y axis specimen of Y axis

[I) a thickness thickness

@ o-2 50 0 - 20 50

0 o-20 50 17 - 38 50

@ / 19 - 40 / 46 37 - 58 46

Same as

AH2

* At the position@ when the stage is moved toward the depth

direction, it interferes with the main body dovetail and the

stage stroke is decreased about 4mm.

Stage: SIC6 Unit: mm

With Nomarski Prism, Without Nomarski Prism

g.5 Observable

Stage

stroke Observable Stage stroke

:::: specimen of Y axis specimen of Y axis

* (4 thickness thickness

0 Not mountable Not mauntable

@ 0 - 2.5 150 O-20:5 150

0 -1*5 - l9 150

19.5 ..,

37 150 Same as

AH2

19 - 22.5

37 "40.5

94 I

* At the

position@,

if the Nomarski prism is attached, it interferes

with the stage, and impossible. (At the position@ a thin L

specimen can be observed, and the position@is unnecessary in

SICG.)

* At the position@ when the stage is moved toward the depth

direction, it interferes with the main body dovetail and the

stage stroke is decreased.

(13) If the stage is set at lower position for observing a thick

specimen, the stage rotation angle is decreased. (At combination

of SVR and SVL) (The decreasing level differs for X and Y positions

of the stage. The rotation angle in the worst condition is about 300.)

(14) If the stage is set lower, the stage handle is close to the base

affecting maneuverability. (At combination of SVR and SVL)

(15) When the halogen lamp other than the specified one is used, the

following conditions are added. (Same as AH2)

@ The lamp optical axis may not be cantered.

@ The lamp life time is not ensured.

A-7

AH3 A. OUTLINE OF PRODUCT

(10) When the halogen lamp other than the specified one is used, the

following conditions are added. (Same as AH2)

1 The lamp optical axis may not be centered.

2 The lamp life time is not ensured.

AHMT3

(1) use the OBJECTIVE and EYEPIECE of the LB series.

(2) Use C-35AD, C-35AD-2 or C-35AD-4 camera. The other 35mm cameras

can not be used.

(3) Allowable supply voltage fluctuation

rt13% of the input voltage

(4) Operating temperature: 0 - 40°C

(5) Wait at least 3 seconds when turning on after it was turned

Off.

(6) Transmitted light observation is possible only when AH2-LWCD

attached to AH2-SIC6. In the standard combination of AH2-SVR

and SVL, the LONGWORKINGDISTANCE CONDENSER(AH2-LWCD) can not

be attached and the transmitted light observation is

impossible.

(7) When setting stage to location other than three screw locations,

ncted below, fix stage securely to the dovetail of the main body.

(8)

The uppermost screw position (0) can not be

(See the drawing below.) used in with AH2-SIC6.

(9) As the specimen load is increased, traceability becomes worse

and the stage bending amount is increased. (S&e level as the

structure is the same as AH-2)

(10) W:-.en the guide dovetail becomes llrrun or less from the top in the

transmitted light illumination, the luminous flux is blurred.

This is no problem in practical use. (Same level as AH-2)

(11) A turret condenser (A2-TCS, A2-TCT, A2-TCS2, A2-TCT2) can not

be attached.

(12)

Ii the stage is set lower than the middle screw position (0)

(-&hen observing a thick specimen), the stage stroke of Y axis

is decreased.

A-6

AH3 A. OUTLINE OF PRODUCT

- Contrast 10% min. in AF sensor range

Contrast = (I max - I min)/(I max/+ I min) x lOO(%)

-...------..___. Imax

'c __-__--_-_.

Imin

- Pattern Not regular pattern

- Color SPECTRALCHARACTERISTICS 460 - 650nm

(6) Wait for 30 minutes at least after the lamp is lit for photo-

grapbing. (When the general magnification is 200X or bigger and

exposure time is 10sec. or more)

(7) DPlanApo40xoilUV can not be used in the Nomarski observation

with AH3-NSF. (Only dry type can be used.)

(8) For the compatibility of each unit, refer to No. A-8 Compatibility.

(9) When the objective of 40X is used, the slide glass may not be

inserted in the still state.

(10) If tbe correction ring is extremely displaced, the AF may not work.

(11) Unless the specimen thickness is within 31.lmin AF, the focus may not

be adjusted on the target point.

(12) When the halogen lamp other than the specified one is used, the

following conditions are added. (Same as AH2).

@ The lamp optical axis may not be centered.

@ The lamp life time is not ensured.

AHBT3

(1) Use the OBJECTIVE and EYEPIECE of the LB series.

(2) Use C-35AD, C-35AD-2 or C-35AD-4 camera. The other 35mm cameras

can not be'used.

(3) Allowable supply voltage fluctuation

210% of the input voltage

(4) Operating temperature: 0 - 40°C

(5) Wait at least 3 seconds when turning on after it was turned

off.

(6) Condenser is not switched at a low magnification (1X m 4X) in the

Nomarski observation with a transmitted light.

(7) Photograph release is ineffective during switching of the condenser,

revolving nosepiece or camera optical path.

(8) Setting and operation of the condenser and revolving nosepiece are

not accepted during photograph releasing.

(9) DPlanApo40xoilW can not be used in the Nomarski observation

with AH3-NSF. (Only dry type can be used.)

A-5

AH3 A. OUTLINE OF PRODUCT

(5) S-iper wide field of view (Field Number 26.5mm)

(6) Friur built-in photographic eyepieces (2.5X, 3.3X, 4X, 5X)

(7) B;lilt-in light balancing filter, ND filter

(8) Provided with the inter-pupillary distance adjustment knob

(9) Specimen of thickness up to 58rmn can be observed.

(Zithout changing the vertical stroke of 21mm)

Specimen of thickness up to 40mm can be observed when using

t?e reflected light Nomarski observation. (When AH2-SIC6

is used, specimen of thickness up to 22mm is same as AH2)

3, USINGCONDITIONS I

AHBS3

(1) Use the OBJECTIVE and EYEPIECE of the LB series.

(2) Use C-35AD-2 or C-35AD-4 camera. The other 35mm cameras can

not be used.

(3) Operating conditions

Temperature: 0 - 40°C

Humidity: 30 - 85%

(4) Allowable supply voltage fluctuation

510% of input voltage

(5) AZ (Auto Focus)

1 TRANSMITTED LIGHT BRIGHT FIELD ILLUMINATICN FOR BIOLOGICAL is

only permitted.

0 AF applicable objectives SPlan 1X _ 40x

SPlanApo 1X - 40X

@ AF sensor range w

Hatched area of the ""I D = Reticule circle

drawing on the right &

$Ihw??wA

I0.3D

diameter

Specimen

- Slide glass thickness 0.8 - 1.4mm

. Minimum specimen size 1X 20pm

2x 10um

10x am

20X l.Oum

40x 0.5pm

. TRANSMITTANCE 3% min. in AF sensor range

Transmittance = (EMERGENTLIGHT / INCIDENT LIGHT) X lOO(%)

A-4

AH3 A. OUTLINE OF PRODUCT

(14)

AHBT3

(1)

(2)

(3)

(4)

(5)

(6)

(7)

(8)

(9)

(10)

(11)

(12)

AHMT3

(1)

(2)

(3)

Difference from AHBS (comparing with AHBS . ..)

* A2-TCS position indication screw is eliminated. (A2-TCS2)

* AZ-BLT CT hole and its cover are eliminated. (A2-6RE2[6REDl)

* line voltage selector switch is eliminated. (AHBS3-F)

* Applicable to install AH3-RS232C (AHBS3-F)

When connecting AH3-RS232C to AHBS, it is necessary to replace

the built-in ROMof AHBSwith ROMof AHBS3 (Option)

When connecting AH2-GPIB-2 to AHBS3, it is also necessary to

replace the built-in ROMof AHBS3with ROMof AHBS

(Option)

In either case, the ROMcan not be replaced by the user.

Built-in auto exposure photographing unit of the 30% AVERAGE/l%

SPOT METERING selectable type

Attachable with three cameras and one TV camera at Same time

Scale and data imprintable and photographing in half size

Motorized REVOLVINGNOSEPIECE

Super wide field of view (Field Number 26.5mm)

Four built-in photographic eyepieces (2.5x, 3.3x, 4x, 5x)

BUILT-IN LIGHT BALANCING FILTER, ND filter

Provided with the inter-pupillary distance adjustment knob

Condenser is switched automatically according to the objective

magnification. Applicable revolving nosepieces are ~2-6RE2

(A2-BTL) and AH3-RFCA. When the condenser is manually switched,

the objective does not moved with it.

When the built-in lamp switch is turned off in observation with

reflected light fluorescence (using AH3-RFCA), the condenser is

not switched even if an objective is changed. (This is the

feature for the user who use the reflected light fluorescence

only and does not use the transmitted light.)

The data setting position of the objective and condenser is

stored even if the main switch is turned off.

This model (~2-AAC-2) has improvement against protection Of self

fluorescence light of A2-AAC in observation with the reflected

light fluorescence

Built-in auto exposure photographing unit of the 30% AVERAGE/

1% SPOT METERING selectable type

Attachable with three cameras and one TV camera at Same time

Scale and data imprintable and photographing in half size

(4) Motorized revolving nosepiece

A-3

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