Olympus Fluoview FV1000 User manual

User’s Manual

FLUOVIEW

FV1000

CONFOCAL LASER SCANNING

BIOLOGICAL MICROSCOPE

[OPERATION] FV10-SW Ver 5.0c

This user’s manual is for the software to be run on Olympus FLUOVIEW FV1000 Confocal Laser

Scanning Biological Microscope. To ensure safety, obtain optimum performance and familiarize

yourself fully with this product, we recommend that you study this manual thoroughly before operation.

This user’s manual is composed of two volumes including “OPERATION INSTRUCTIONS” and

“MAINTENANCE”. Together with this manual, please also read the “SAFETY GUIDE”, “HARDWARE

GUIDE” of User’s manual FLUOVIEW FV1000 and the instruction manual of the microscope in order to

understand overall operation methods. To ensure the safety operation of laser system, we recommend

you to study the manual of each laser and the light source equipment besides this manual.

Retain this manual in an easily accessible place near a system for future reference.

Petition

X7274

CAUTION

Page

CAUTION

1. Reproduction, copying or duplication of a part or all of this software and manual is prohibited.

2. The information described in this manual may be subject to change without notice.

Registered Trademarks

Microsoft, Microsoft Windows, Excel for Windows are registered trademarks of Microsoft Corporation.

Other brand names and product names are trademarks or registered trademarks of their respective owners.

FLUOVIEW MANUAL CONFIGURATION

2Page

FLUOVIEW MANUAL CONFIGURATION

The FLUOVIEW system uses two manuals including this “User’s Manual” and the on-screen

manual built into the software (“Online Help”).

The User’s Manual is composed of the five following volumes and subject matter:

OPERATION INSTRUCTIONS

Describes the operation procedures of the FLUOVIEW system, for example, methods

for image acquisition and various image processing.

1 Getting Started FLUOVIEW................................................................................ 1-1

2 APPLIED OPERATIONS.................................................................................... 2-1

Appendix A List of Hot Keys.................................................................................. A-1

Appendix B Glossary ............................................................................................. B-1

Appendix C USER REGISTRATION OF FV1000 ................................................. C-1

Appendix D Change of Default Folder for [File I/O] Panel..................................... D-1

Appendix E List of Functions in the [Active Overlays] Dialog Box......................... E-1

Appendix F Hand Switch and Microscope Frame Function Allocation .................. F-1

MAINTENANCE

Describes maintenance of the FLUOVIEW system.

1 Software Setup................................................................................................... 1-1

2 Maintenance of Major System Units................................................................... 2-1

3 Setting the Confocal Aperture ............................................................................ 3-1

TROUBLESHOOTING

Describes countermeasures in case trouble occurs.

1 TROUBLESHOOTING GUIDE ........................................................................... 1-1

For Online Help, please see “1-3 Online Help” in “OPERATION INSTRUCTIONS” of this

manual.

NOTATIONS IN THIS MANUAL

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NOTATIONS IN THIS MANUAL

This manual complies with the following notations.

Notation of Caution, Notes and Tips

Notation Description

Caution to prevent injuries to the user or damage to the

product (including surrounding objects).

NOTE

Note for the user.

TIP

Hint or one-point advice for user reference.

Notation of panel, Command Buttons and Dialog Boxes

Notation Description

[Acquire] panel The name of a panel, dialog box, list box or check box is

enclosed inside square brackets ([ ]).

<OK> button

<Open File> button

The name of a button in a panel or dialog box is enclosed

inside angled brackets (< >).

Notation of Mouse Operations

Notation Description

clicking Action of pressing, then immediately releasing the mouse

button.

double-clicking Action of clicking the mouse button twice in quick succession.

dragging Action of moving the mouse while holding down the mouse

button, then releasing the mouse button at the desired

destination.

(Note) In this manual, clicking, double-clicking and dragging involves pressing the left button

of the mouse, unless otherwise specified.

NOTATIONS IN THIS MANUAL

4Page

Notation of key operations

Notation Description

Enter The name of a key is enclosed inside .

Alt The positive sign (+) expresses the combination of more than

one key operation.

For example, F1

Alt refers to pressing the

F1 key while holding the Alt key down.

Direction keys Generic names given to the →, ←, ↑and

↓keys.

Notation of system-specific terms

Notation Description

XY observation

(Other observations)

Refers to observation with XY scanning.

(The same principle also applies to other observations such

as XZ, Xt, XYZ, XYt and XYZt.)

Note that some of the panels and dialog boxes shown in this manual are not the precise

reproductions of the originals. Some windows are resized to facilitate the reading and some

grayed-out characters are printed in readable characters.

Software Functional Configuration

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Software Functional Configuration

This software uses panel-type windows.

Usually, it is required to “select a menu then select the command to be executed” in order to

execute a function provided by software. With the panel system, a software function can be

executed easily by “selecting the panel page tab of the function to be executed”, just like

when using a system notebook or file folder.

Function Window and Image Window

The FLUOVIEW software is organized by two kinds of windows, the function windows and

the image .window.

The function windows include the [Acquire], [File I/O], [Tile], [Process], [Analyze] and

[Visualize] panels.

The image .window shows either the [Live] panel or the panel image loaded from a file

([(filename)] window).

In this manual, the function windows are referred to simply using their

page tabs.

Namely, the [File I/O] panel of function windows is referred to simply as the

[File I/O] panel.

NOTE

The function window

The image window

Software Functional Configuration

6Page

Panel Structure of the Software

This software cannot show the all function panels at a glance.Please use the following list of

the panels for reference in scrolling.

Acquire

Settings

Z Stage

Sets up the image acquisition and executes actual acquisition.

Sets the zooming ratio and observation mode for image acquisition.

Sets the microscope light path for image acquisition.

Sets the Z-direction scanning range for image acquisition.

Time Series Sets the interval period for image acquisition.

File I/O

Tile

Process

Math

Filters

Histogram

Experiment Editor

Analyze

Single

Saves, loads and deletes images.

Changes the image display method.

Processes the acquired images.

Performs mathematical and logical operations between images.

Filters images.

Changes the image contrast.

ppends two images, adds and extracts the channel.

nalyzes image data.

Obtains the intensity values, intensity distribution, length, area and

average intensity in images.

Orientation

Other Options

Series Obtains the change in the sum of intensity values in images.

Visualize Constructs an image from a different viewpoint or displays a 3D image.

Sets the image rotation angle, direction and number.

Sets the various 3D Rendering.

Dyes Sets the fluorescence dye method for image acquisition and reagent for

each channel.

Lasers Sets the intensity of laser and other option for image acquisition.

Software Functional Configuration

Page

Icons Executed by Dragging & Dropping

This software selects image files and observation methods (dye name) by means of

dragging & dropping. This allows simple selection based on an intuitive operation of

“selecting an icon (image file or observation method), dragging it to the desired position and

dropping it there”.

Software Functional Configuration

8Page

Identification of Images Depending on the Observation Methods

On many occasions, FLUOVIEW displays image

icons to allow identification of the observation

method used when each image is acquired. (See

table on the left.)

When the [File I/O], [Tile], [Process], [Analyze] or

[Visualize] panel is selected, the icon of the image

selected in the image window is displayed in a

frame at the top of the function panel. The image

icons are also displayed in the [Icon] field in the

[Files] list box in the [File I/O] panel or during

dragging of an image file.

Use these icons to identify the observation methods

used in image acquisition.

In all observation modes, the icons for 3

or more channels are identical.

Image Icon Significance

XZ observation

XZ observation, 2-channel mode

Xt observation

Xt observation, 2-channel mode

XZT observation

XZT observation, 2-channel

mode

XY observation

XY observation, 2-channel mode

XYt observation

XYt observation, 2-channel

mode

XYZ observation

XYZ observation, 2-channel

mode

XYZt observation

XYZt observation, 2-channel

mode

Point Scan

Animation image

Stereo 3D image: Image to be

viewed with color eyeglasses.

3 or more channels

TIP

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