Olympus SZX16 User manual

INSTRUCTIONS

A X 7 5 0 0

Modules described in this manual

SZX16

SZX-R/SZH-P400/SZH-P600

SZX-2-2RE16

SZX-STAD1

SZX2-AN

SZX-PO

SZX-STAD2

SZH-STAD1

SZX16

RESEARCH HIGH-CLASS STEREO

MICROSCOPE

This instruction manual is for the Olympus SZX16 Research High-Class Stereo Microscope System.

To ensure the safety, obtain optimum performance and to familiarize yourself full with the use of this

microscope, we recommend that you study this manual thoroughly before operating the microscope.

Retain this instruction manual in an easily accessible place near the work desk for future reference.

This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic

medical devices. CE marking means the conformity to the directive.

SZX16

CONTENTS

IMPORTANT — Be sure to read this section for safe use of the equipment. —

1 NOMENCLATURE

2 CONTROLS

3 SUMMARY OF OBSERVATION PROCEDURE

4 USING THE CONTROLS

1-3

7-13

4-1 Base ...................................................................................................................................................................................................................... 7

1Using the Stage Plate 2Placing the Specimen

4-2 Microscope Body and Focusing Assembly ......................................................................................... 7-9

1Adjusting the Tension of the Coarse Focus Adjustment Knob

2 Engaging and Disengaging the Zooming Knob Click Stop Function

3Adjusting the Aperture Iris Diaphragm 4Zoom Magnification Indication

52X Objective Correction Collar

4-3 Observation Tube.................................................................................................................................................................. 10-12

1Adjusting the Interpupillary Distance

2Adjusting the Diopter (Zoom Parfocal Adjustment)

3Using the Eye Shades 4Using the Eyepiece Micrometer Disk

5Selecting the Light Path 6Adjusting the Tilt

4-4 TV Observation and Photomicrography ........................................................................................................ 13

1Selecting the TV Adapter Magnification 2Mounting the TV Adapter

3Selecting the TV Camera Light Path

6 SPECIFICATIONS

OBSERVATION MAGNIFICATIONS AND OBSERVATION AREAS

5 TROUBLESHOOTING GUIDE 14

15,16

8 ASSEMBLY

9 OPERATION OF OTHER MODULES

9-1

Drop Prevention Collar SZX-R and Auxiliary Pillar SZH-P400/SZH-P600

........ 22

9-2 Revolving Nosepiece SZX2-2RE16.......................................................................................................... 23-25

9-3 BX Stage Adapter Type 1 SZX-STAD1 ................................................................................................. 26-28

9-4 BX Stage Adapter Type 2 SZX-STAD2 ................................................................................................. 29-30

9-5 Stage Adapter Type 1 SZH-STAD1 ...................................................................................................................... 30

18-21

22-30

SZX16

IMPORTANT

SAFETY PRECAUTIONS

1. After the equipment has been used in an observation of a specimen that is accompanied with a potential of infection,

clean the parts coming in contact with the specimen to prevent infection.

· Moving this microscope is accompanied with the risk of dropping the specimen and causing infections. Be sure to

remove the specimen before moving this product.

· In case the specimen is damaged by erroneous operation, promptly take the infection prevention measures.

· The microscope may become unstable when certain intermediate attachments and/or photography unit are mounted

on it. Take the measures so that the microscope does not turn over.

Fig. 1

2. To prevent the microscope body from turning over, its left-right pivot angle

must be limited to 30° as shown in Fig. 1. The tilt of the desktop surface

should be no more than 5°.

3. Sufficient care is required during observation with a low-magnification

objective, because the objective’s long WD (Working Distance) can

displace the microscope body to a higher position.

The measures for prevention of turning over are also required when using

an auxiliary pillar (SZH-P400/P600), because this also makes the

microscope body to be displaced to a higher position.

4. To adjust the microscope body height, be sure to hold the microscope

body with one hand while loosening the focusing assembly clamping

knob @. (Fig. 1)

(Use the drop prevention collar (SZX-R) to prevent a hazard from occurring.)

Be careful not to pinch your finger during the adjustment.

1Getting Ready

1. A microscope is a precision instrument. Handle it with care and avoid

subjecting it to sudden or severe impact.

2. Do not use the microscope where it is subjected to direct sunlight, high

temperature and humidity, or vibration. (For the operating environment,

see Chapter 6, “SPECIFICATIONS” on page 16.)

3. When moving the microscope, remove the specimen in advance. Then,

hold the front part of the base with one hand and hold the rear part of the

base with the other hand to avoid tilting the microscope.

Also remove any module or attachment from the microscope to minimize

the weight before moving.

4. Observe the following cautions when operating the coarse focus

adjustment knob or the zooming knob.

Operation

Manipulated Controls

Caution

Focusing Coarse/fine focus

adjustment knobs @

(Fig. 3)

1. If the knob hits the upper or lower

limiting mechanism violently or

it is rotated after it hits a limiting

mechanism, the internal

mechanism may be damaged.

2. If the knobs on the left and right

are rotated in opposite directions,

the internal mechanism will be

damaged. (The rotation tension

of the coarse focus adjustment

knob should be adjusted using

the rotation tension adjustment

ring ³ on the knob.)

Zooming Zooming knob ²

(Fig. 3) 1. If the knob hits the upper or lower

limiting mechanism violently or

it is rotated after it hits a limiting

mechanism, the internal

mechanism may be damaged.

2. If the knobs on the left and right

are rotated in opposite directions,

the internal mechanism will be

damaged.

Fig. 2

Fig. 3

5. Only one intermediate attachment can basically be used, but two

attachments may also be used though the peripheral part of the image

may be obscured.

For the coaxial vertical illuminator (SZX2-ILLC16) and reflected fluorescent

light illuminator (SZX2-RFA16), only either can be attached because they

must always be attached at the lowest position.

}The SZX2-ILLC16 is not considered to be an attachment. Therefore, the

SZX2-LBS can be mounted above it without any restriction.

²³@

SZX16

2Maintenance and Storage

1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe

gently using a piece of cleaning paper (or clean gauze).

If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute

alcohol.

Since the absolute alcohol is highly flammable, it must be handled carefully.

Be sure to keep it away from open flames or potential sources of electrical sparks -- for example, electrical

equipment that is being switched on or off.

Also remember to always use it only in a well-ventilated room.

2. The equipment uses plastic resins extensively in its external finish. Do not attempt to use organic solvents to clean them

but simply wipe them using a lint-free, soft cloth. To clean an extremely dirty part, use a soft cloth lightly moistened with a

diluted neutral detergent.

3. Never disassemble any part of the microscope as this could result in malfunctions or reduced performance.

4. When not using the microscope, keep it covered with the dust cover provided. If a module containing a source of heat is

mounted on the microscope, wait until it has cooled down before covering.

5. This equipment should be disposed of by following the rules and regulations of your national or local government.

3Caution

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,

the microscope may also be damaged. Always use the microscope as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

: Indicates that failure to follow the instructions in the warning could result in bodily harm to the

user and/or damage to equipment (including objects in the vicinity of the equipment).

# : Indicates that failure to follow the instructions could result in damage to equipment.

} : Indicates commentary (for ease of operation and maintenance).

4Intended use

This instrument has been designed to be used to observe magnified images of specimens in routine and research

applications.

Do not use this instrument for any purpose other than its intended use.

Eyepieces

{WHN10X-H

· WHSZ15X-H

· WHSZ20X-H

· WHSZ30X-H

NOMENCLATURE

}The following illustration shows a typical system composed of modules marked with “{” in the list of each module, and

other modules may also be used in place of them. For the modules that are not shown in the module lists below, please

contact Olympus or the latest catalogues.

Microscope

body

{16X zoom microscope

body: SZX2-ZB16

Objective

· SDFPLFL0.3X**

· SDFPLAPO0.5XPF

· SDFPLAPO0.8X

{SDFPLAPO1XPF

· SDFPLAPO1.6XPF

· SDFPLAPO2XPFC

Observation tube

{Trinocular observation

tube: SZX2-TR30/TR30PT

· Tilting trinocular observation

tube: SZX2-TTR/TTRPT

Focusing assembly

· Coarse/fine focusing assembly:

SZX2-FOF

· Focusing assembly: SZX2-FO

{Coarse/fine focusing assembly

for heavy load: SZX2-FOFH

· Motorized focusing assembly:

SZX2-FOA2

Intermediate

attachments

· Coaxial vertical illuminator:

SZX2-ILLC16*

· Beam splitter: SZX2-LBS*

· Vertical fluorescent light

illuminator: SZX2-RFA16*

Base

{Standard base: SZX2-ST**

· Large base: SZX2-STL

· Transmitted light base: SZX2-ILLB*

SZX2-ILLD*

SZX2-ILLK*

SZX2-ILLT*

Stage plate

{Monochrome plate: SZ2-SPBW/SP-BW2

· Glass plate: SP-C

· Fluorescence center plate: SP-FL

*A separate instruction manual is available for the module marked *.

**The standard base (SZX2-ST) requires use of the optional auxiliary pillar (SZH-P400) and optional drop prevention collar

(SZX-R). The large base (SZX2-STL) comes with the auxiliary pillar (SZH-P400) mounted as standard. This enables the

large base to be used as is. However, be sure to use the drop prevention collar (SZX-R) in combination.

SZX16

CONTROLS

}If the microscope is not yet assembled, see Chapter 8, “ASSEMBLY” (pages 18 to 21) before the following.

Diopter adjustment ring (P.10)

±5m-1 (per meter)

Eyepiece clamping knob

Aperture iris diaphragm ring (P. 8)

Zoom magnification indication (P. 9)

0.7 to 11.5 (14 indications)

Click stop ON-OFF screw (P. 8)

Engages or disengages the click stop

function for each zoom magnification.

Light path selector knob (P. 12)

Zooming knob

0.7X to 11.5X

Coarse focus adjustment knob

tension adjustment ring (P. 7)

Fine focus adjustment knob

(SZX2-FOF/FOFH)

Stroke: 80 mm

Stroke per turn: 0.77 mm

Coarse focus adjustment knob

Total Stroke: 80 mm

Stroke per turn (SZX2-FOF/FOFH): 36.8 mm

Stroke per turn (SZX2-FO): 21 mm

Specimen holder

Locks the specimen.

Focusing assembly

clamping knob

SUMMARY OF OBSERVATION PROCEDURE

3-1 Preparation

Ref. Page

1. Check and tighten the connection of each component, especially the observation tube................................................... (Page 20)

2. Check that the angled formed by the microscope body with respect to the base is less than the

turning-over prevention angle. .................................................................................................................................................................................................................................(Page 19)

3. Adjust the tension of the coarse focus adjustment knob. .................................................................................................................................................. (Page 7)

4. Confirm the correct settings.

3-2 Observation Procedure

1. Place a specimen on the stage. (Page 7)

2. Adjust the interpupillary distance. (Page 10)

3. Make diopter adjustment of the eyepieces. (Page 10)

(The adjustment procedure is variable depending on whether the eyepiece

micrometer disk is used or not.)

4. Set the zooming knob @ to the lowest zoom magnification and bring the

microscope into focus by rotating the coarse focus adjustment knob ².

5. Rotate the zooming knob @ to the desired magnification and precisely

focus the microscope on the specimen with the coarse focus adjustment

knob ² and fine focus adjustment knob ³ (the fine focus adjustment

knob is not provided with the SZX2-FO).

}The contrast of the observed image and the focal depth of the specimen

can be adjusted with the aperture iris diaphragm ring.

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