Olympus U-CBE User manual

A X 5 8 9 4

INSTRUCTIONS

COMPENSATORS

This instruction manual is for the Olympus Compensators. As this manual pertains only to the compensators,

also refer to the instruction manual for your polarizing microscope (BX53/BX53M-P or CX31-P) so that you

understand the operating procedures of the entire system.

To ensure the safety, obtain optimum performance and familiarize yourself fully with the use of these units,

we recommend that you study this manual thoroughly before using them.

Retain this instruction manual in an easily accessible place near the work desk for future reference.

Optical Microscope Accessory

IMPORTANT

These units employ a UIS2/UIS (Universal Infinity System) optical design, and should be used only

with a microscope, eyepieces, objectives and condenser matching the UIS/UIS2 optics.

Less than optimum performance may result if inappropriate accessories are used.

1Getting Ready

1. A compensator is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe

impact.

2. Do not use the compensator where it is subjected to direct sunlight, high temperature and humidity,

dust or vibrations.

(Working environment ambient temperature and humidity should be in the range of 0-40 °C and 30-

90%, respectively. Temperature at storage location should never be allowed to get below -10 °C).

1. To clean the lenses and other glass components, simply blow dirty away using a commercially

available blower and wipe gently using a piece of cleaning paper (or clean gauze). If a lens is stained

with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute

alcohol.

!Since the absolute alcohol is highly ﬂammable, it must be handled carefully.

Be sure to keep it away from open ﬂames or potential sources of electrical sparks --- for

example, electrical equipment that is being switched on or off.

Also remember to always use it only in a well-ventilated room.

2. Do not disassemble any part of the compensator.

If the equipment is used in a manner not specified by this manual, the safety of the user may be imperiled.

In addition, the equipment may also be damaged. Always use the quipment as outlined in this instruction

manual.

2Care and Storage

3Caution

1 OUTLINE 1

2 NOMENCLATURE 2

3 ASSEMBLY 5

4 PREPARATION AND MEASUREMENT 7

4-1

Berek Compensator (U-CBE), .............................................................................................................................................................................7

Thick Berek Compensator (U-CTB)................................................................................................................................................................7

4-2 Brace-Köhler Compensator (U-CBR1, U-CBR2)............................................................................................................11

4-3 Quarts Wedge (U-CWE2)....................................................................................................................................................................14

4-4 Senarmont Compensator (U-CSE).............................................................................................................................................16

CONTENTS

1OUTLINE

Compensators are used for measuring the retardation in specimens with double refraction. Some

compensators* may also be used to enhance image contrast in polarized light observation. The following

table shows the retardation measuring range of the various compensators.

Measuring Range of Compensators

Compensator Name Measuring Range

Thick Berek (U-CTB) 0 -- 20 λ

Berek (U-CBE) 0 -- 3 λ

Quartz Wedge (U-CWE2) 1 -- 4 λ

* Senarmont Compensator (U-CSE) 0 -- 1 λ

* Brace-Köhler Compensator 1/10 λ(U-CBR1) 0 -- 1/10 λ

* Brace-Köhler Compensator 1/30 λ(U-CBR2) 0 -- 1/30 λ

λ= 546.1 nm (e-line)

U-CBE, U-CTB

2NOMENCLATURE

Berek Compensator (U-CBE)

Thick Berek Compensator (U-CTB)

Compensator

Scale 2

One turn equals 10°

(minimum reading 0.05°)

Compensator knob

Scale 1

0-60°

(minimum reading 10°)

* The illustration shows the U-CTB. The U-CBE’s γaxis direction is different.

U-CBR1, U-CBR2

Brace-Köhler Compensator

(U-CBR1, U-CBR2)

Compensator Scale 2

One turn equals 20°

(minimum reading 0.1°)

Compensator knob

Scale 1

40-0-40°

(minimum reading 20°)

* The illustration shows the U-CBR2. The U-CBR1 is the model with the same design

as the U-CBR2 but only the product name is different.

U-CWE2, U-CSE

Quartz Wedge

(U-CWE2)

Senarmont Compensator

(U-CSE)

Compensator

Retardation indication

} The assembly method is the same for all the compensators.

The compensator is designed to be inserted in the test plate

adapter (U-TAD) or polarization attachment (U-PA).

With U-TAD

1. Loosen the clamping knob @ at the front of the revolving

nosepiece and remove the dummy slider.

2. Insert the test plate adapter (U-TAD) ² and tighten the

clamping knob @.

3. Loosen the test plate adapter clamping knob ³ and insert

the compensator | as far as the click-stop position (where

the compensator is not engaged into the light path), then

tighten the clamping knob ³.

With U-PA

1. Disengage the Bertrand lens from the light axis.

2. Engage the analyzer in the light path and set the rotary scale

to position “0”.

3. Push in the compensator as far as it will go, and then pull it

slightly out until it stops in the click position.

# If the compensator is inserted at a tilted position, it

may impinge against the insertion slot. Always set to

position 30°.

} When measuring, insert the compensator one step further

to position it in the light path.

Preparation for Measuring

} Preparation for measuring is the same for all compensators.

Fig. 1

3ASSEMBLY

Perform the cross-Nikol adjustment and the eyepiece cross

line alignment as described in the instruction manual for the

polarizing microscope (BX53/BX53M-P or CX31-P).

Note on Measuring:

} Measuring is usually performed with the condenser’s top-lens swung out and the aperture iris

diaphragm stopped down. If it is required to maintain brightness and resolution, the top-lens should

be left engaged although measuring accuracy will decrease.

} When measuring retardation, backlash error may occur when the compensator knob is rotated (U-CBE,

U-CTB, U-CBR1, U-CBR2). Accordingly, keep the rotation direction always uniform. If the knob is rotated

too far, turn it fully back and restart rotation by keeping the direction uniform till the measured portion.

1Adjusting the Extinction

U-CBE, U-CTB

4PREPARATION AND MEASUREMENT

1. Position the specimen on the rotatable stage (U-SRP) and

focus on the specimen.

} Do not use the interference filter in this adjustment.

1. Rotate the stage until it attains the position of extinction

(position where the observed portion of the specimen looks

darkest).

2. Rotate the stage further by +45° and then clamp it.

3. Rotate the compensator knob and set the U-CBE or U-CTB

scale to position 30°.

4. Insert the U-CBE or U-CTB into the test plate adapter (U-TAD)

or polarization attachment (U-PA) as far as it will go.

Measuring

} If an interference filter (IF 546 or IF 550) is available, use of

this filter will improve measurement accuracy.

1Adjusting the Extinction

4-1

Berek Compensator (U-CBE), Thick Berek Compensator (U-CTB)

2Adjusting the Rotation Position

Fig. 2-1

Fig. 2-2

Interference

fringe

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