Thermo Scientific Evolution 300 User manual

Evolution™ 300 and Evolution 600

User Guide

The information in this publication is provided for reference only. All information

contained in this publication is believed to be correct and complete. Thermo Fisher

Scientific shall not be liable for errors contained herein nor for incidental or consequential

damages in connection with the furnishing, performance or use of this material. All

product specifications, as well as the information contained in this publication, are subject

to change without notice.

This publication may contain or reference information and products protected by

copyrights or patents and does not convey any license under our patent rights, nor the

rights of others. We do not assume any liability arising out of any infringements of patents

or other rights of third parties.

We make no warranty of any kind with regard to this material, including but not limited

to the implied warranties of merchantability and fitness for a particular purpose.

Customers are ultimately responsible for validation of their systems.

may be stored in a retrieval system, transmitted, or reproduced in any way, including but

not limited to photocopy, photograph, magnetic or other record, without our prior written

permission.

For technical assistance, please contact:

Technical Support

Thermo Fisher Scientific

5225 Verona Road

Madison, WI 53711-4495

U.S.A.

Telephone: 1 800 532 4752 (U.S.A.) or +1 608 273 5017 (worldwide)

Fax: +1 608 273 5045 (worldwide)

E-mail: [email protected]

World Wide Web: http://www.thermo.com/spectroscopy

Microsoft, Windows and Windows NT are either trademarks or registered trademarks of

Microsoft Corporation in the United States and/or other countries. Spectralon is a

registered trademark of Labsphere, Inc. All other trademarks are the property of Thermo

Fisher Scientific Inc. and its subsidiaries.

269-165900, Rev. B

Contents

Introduction.........................................................................................1

Conventions used in this manual .............................................................. 2

Questions or concerns............................................................................... 3

Spectrophotometer Basics ....................................................................4

Spectrophotometer components................................................................ 4

Connectors ............................................................................................ 5

Accessories............................................................................................8

Manual accessories .................................................................................... 8

Installation............................................................................................. 9

Standard rectangular cell holder ........................................................... 10

Adjustable micro cell holder.................................................................10

Long pathlength rectangular cell holder ............................................... 12

Cylindrical cell holder.......................................................................... 13

Thermostatted single cell holder .......................................................... 13

Test tube/vial holder ............................................................................ 15

Rectangular cell holder......................................................................... 15

Fiber optic sampling accessory ............................................................. 16

DRA-EV-600 Diffuse Reflectance accessory ........................................18

Solid Sample Holder............................................................................ 19

Smart Accessories .................................................................................... 20

System accessories ................................................................................... 21

Installing Smart Accessories..................................................................... 22

Using the Instrument .........................................................................25

Local Control.......................................................................................... 25

Main Menu page .................................................................................27

Text entry ............................................................................................ 37

Editing numeric fields..........................................................................38

Pop-up menus...................................................................................... 39

File operation....................................................................................... 39

Scan application................................................................................... 41

Fixed application.................................................................................. 45

Quantitation application...................................................................... 48

Kinetics application.............................................................................. 50

Multicomponent application ............................................................... 54

Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide i

ii Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific

Biological tests .....................................................................................56

Using UVcalc .......................................................................................68

Instrument verification tests .................................................................71

VISIONsecurity and VISIONpro.............................................................74

Maintenance.......................................................................................75

Routine maintenance ..............................................................................75

Cleaning instrument exterior...................................................................75

Internal printer........................................................................................76

Light sources (Evolution 600 only) .........................................................78

Deuterium lamp ..................................................................................80

Tungsten-halogen lamp .......................................................................81

Mercury lamp (Evolution 300 and Evolution 600) ..............................82

Introduction

Congratulations on your purchase of an Evolution™ 300 or Evolution 600

spectrophotometer! Our spectrophotometers integrate advanced hardware

features with the power and flexibility of a wide range of Smart

Accessories™.

Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 1

Conventions used in this manual

This manual includes safety precautions and other important information

presented in the following format:

Note Notes contain helpful supplementary information. 

Notice Follow instructions labeled “Notice” to avoid damaging the system

hardware or losing data. 

Caution Indicates a hazardous situation which, if not avoided, could result in minor

or moderate injury. 

Warning Indicates a hazardous situation which, if not avoided, could result in death

or serious injury. 

Danger Indicates a hazardous situation which, if not avoided, will result in death or

serious injury. 

2 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific

The following safety symbols are used on this product:

Symbol Description Indication

Black graphical

symbol inside a

yellow triangle with a

black triangular band

This is a warning symbol. The graphic in

this symbol is used to alert the user to

potential hazards.

Black graphical

symbol inside a red

circular band with a

red diagonal bar

This is a prohibition symbol. The graphic in

this symbol is used to alert the user to

actions that shall not be taken or shall be

stopped.

White graphical

symbol inside a blue

circle

This is a mandatory action symbol. It is

used to indicate that an action shall be

taken to avoid a hazard.

Black graphical

symbol inside a

yellow triangle with a

black triangular band

This is the general warning sign. Failure to

heed the safety precautions could result

in personal injury.

White graphical

symbol inside a blue

circle

This is the general data loss or property

damage symbol and is not related to

personal injury. Failure to heed these

precautions can result in irreparable

damage to property or permanent data

loss.

Questions or concerns

In case of emergency, follow the procedures established by your facility. If

you have questions or concerns about safety or need assistance with

operation, repairs or replacement parts, you can contact our sales or service

representative in your area or use the information at the beginning of this

document to contact us.

Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 3

Spectrophotometer Basics

This chapter describes the major components of your spectrophotometer.

Spectrophotometer components

The following illustration identifies some major components visible on the

outside of a typical Evolution spectrophotometer. (Some components may

not be present on your spectrophotometer.)

Contrast

Keypad

LCD screen

Zero

Run

789

456

123

Optional printer

Floppy di

drive

Sliding door

Sample

compartment

ort for optional

power supply

4 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific

Connectors

The following illustrations show the locations of the connectors on each

side of the spectrophotometer.

Right side

Zero

Run

789

456

123

PRINTER KEY BD EXT ACCY RS232 DISPLAY

EXT

Connector Function

Printer Use to connect a printer with a parallel interface.

Key Bd Use to connect an English keyboard (this is supplied by

us to meet electrical emission requirements).

Ext Use to connect an external TTL (or contact closure)

trigger.

Accy Use to connect an external accessory.

RS232 Use to connect a computer with an RS232 interface.

Ext Display Use to connect to a computer monitor.

Thermo Fisher Scientific Evolution 300 and Evolution 600 User Guide 5

Left side

Port for optional

Peltier power supply

Power ON/OFF switch

Power supply

connection

Power cord

Zero

Run

789

456

123

Danger Avoid shock hazard. Always power off the spectrophotometer and

disconnect the power supply from the wall outlet or power strip before

disconnecting the power supply from the spectrophotometer. 

Inside sample compartment

The following illustrations show the locations of the connectors inside the

sample compartment.

Evolution 300

Reference

connector

Sample

connector

6 Evolution 300 and Evolution 600 User Guide Thermo Fisher Scientific

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