OPTO-EDU A12.0910 User manual

A12.0910

Research Scientific Laboratory Microscope,

Semi-APO, Upgrade BF+DF+PL+PH+FL+DIC

Instruction Manual

This instruction manual is for the operation guide, troubleshooting and

maintenance to the A12.0910 series biological microscope. Please study this

manual thoroughly before operating, and keep it with the instrument. The

manufacturer reserves the rights to the modifications by technology

development. On the basis of operation ensured, technical specifications

may be subject to changes without notice.

 
 
Contents A12.0910 
Before Use 
1. Components..................................................................................................................................1 
2. Assembling....................................................................................................................................5 
2-1 Assembling Scheme ............................................................................................................5 
2-2 Assembling Steps................................................................................................................7 
3. Operation....................................................................................................................................10 
3-1 Set Illumination ................................................................................................................10 
3-2 Place the Specimen Slide..................................................................................................10 
3-3 Select the Light Path.........................................................................................................11 
3-4 Adjust Focusing................................................................................................................11 
3-5 Adjust the Focusing Tension ............................................................................................12 
3-6 Adjust the Interpupillary Distance ...................................................................................12 
3-7 Use the Eye-cap................................................................................................................12 
3-8 Adjust Stage......................................................................................................................12 
3-9 Center the Condenser.......................................................................................................14 
3-10 Adjust the Field Diaphragm...........................................................................................14 
3-11 Adjust the Aperture Diaphragm .....................................................................................15 
3-12 Use the Color Filter .......................................................................................................15 
3-13 Replace the Fuse ............................................................................................................16 
3-14 Assemble and Use the TV Device...................................................................................16 
4. Assembling and Operation of Fluorescence Illuminator............................................................17 
4-1 Assemblage Steps .............................................................................................................17 
4-2 Operation .........................................................................................................................21 
5. Troubleshooting..........................................................................................................................26 
5-1 Solutions of A12.0910 Series Biological Microscope Problem........................................26 
5-2 Solutions of Mercury Bulb Fluorescent Device Problem .................................................28 
 
 

Before Use A12.0910

1. Operation Notice 1. As the microscope is a high precision instrument,

always operate it with care, and avoid physical shake

during the operation.

2. Do not expose the microscope in the sun directly,

either not in the high temperature, damp, dust or acute

shake. Make sure the worktable is flat and horizontal.

Following environment is required when operating:

Indoor temperature: 5 ℃ ～ 40 ℃, Max relative

humidity: 80%.

3. When moving the microscope, use both hands to

hold its back hand-clasping ①and the front base ②,

and lay it down carefully (see Fig. left).

★It will damage the microscope by holding the

stage, focusing knob, head or light source when

moving.

4. When working, the surface of light source will be

very hot. Make sure there is enough room for the heat

dissipating around the light source.

5. Connect the microscope to the ground to avoid

lightning strike.

6. For safety, make sure the power switch is at “O”

(off) and power it off before replacing the bulb or fuse

(see Fig. left).

7. Wide voltage range is supported as 100~240V.

Additional transformer is not necessary. Make sure the

voltage is in this range.

8. Use the special wire supplied by our company.

2. Maintenance

1. Wipe the lens gently with a soft tissue. Carefully wipe off the oil marks and fingerprints on the

lens surfaces with a tissue moistened with a small amount of 3:7 mixture of alcohol and ether or

dimethylbenzene.

★As the alcohol and ether is flammable, don’t place these chemical near to fire or fire

source. For example, when turning on or turning off the electrical device, please use these

chemical in a ventilated place.

2. Don’t use organic solution to wipe the surfaces of the other components. Please use the neutral

detergent if necessary.

3. If the microscope is damped by liquid, please power it off immediately and wipe it dry.

4. Never disassemble the microscope, otherwise the performance will be affected or the

instrument will be damaged.

5. After using, cover the microscope with a dust cover.

3. Safety Sign

Sign

Signification

The surface gets hot and don’t touch it with bare hand.

Study the instructions before use. Unsuitable operation would lead to

person hurt or instrument faulty.

Main switch ON

Main switch OFF

1. Components A12.0910

A12.0910 Series Biological Microscope Components

Objective

Trinocular head

Light Path

Selecting Pole

Inner

Hexagon

Spanner

Body

Voltage

Indicator

Light

Source

Group

Reset Button

For Intensity

Mechanical

Stage

Condenser

Illuminator

Light

Adjustment

Knob

Tension

Adjustment

Knob

Switch Lever

For Filters

Y-axis Moving

Knob

X-axis Moving

Knob

A12.0910

Fine

Focusing

Knob

Coarse

Focusing

Knob

Condenser

up-down knob

Dust Cover

Coarse Adjusting

Limit Ring

Nosepiece

Objective

A12.0910

A12.0910FL Series Biological Fluorescence Microscope Components

Trinocular

head

Flashboard

knob

XY Body

Vertical centering

Screw

Blinkers

Board

Field Diaphragm

Adjusting Pole

Aperture Diaphragm

Adjusting Pole

Lens Focusing Knob

Diaphragm

Centering Screw

Horizontal

centering Screw

Power Supply Box

Vertical

Fluorescence

Illuminator

A12.0910

ND Filter Slot

2. Assembling A12.0910

2-1 Assembling Scheme

Following is theAssembling Scheme, and the numbers denote the assembling order.

★Before assembling, make sure there is no dust, dirt or other matters to affect the

assembly. Assemble carefully and do not scrap any part or touch the glass surface.

1. A12.0910 Series Biological Microscope Assembling Scheme

A12.0910

2. A12.0910 Series Biological Fluorescence Microscope Assembling Scheme

A12.0910

2-2 Assembling Steps

2-2-1 Assemble and Replace the Halogen Bulb

(1) Loose lock-screw ①completely by M4 spanner ,

then take off the cover group. (See Fig. 1)

(2) Open the bulb lock piece②, and take the bulb③

with clean glove or soft tissue, insert the bulb pins④

into the jack on the bulb holder⑤, the bulb should be

vertical after assembly. (See Fig. 2)

(3) Loose the bulb lock piece②, lock the bulb and

install the cover group.

★Don’t touch the bulb with fingers. If there is a

fingerprint left on the bulb, please wipe it with

clean soft cloth.

★Bulb selected only: 12V/100W Halogen bulb

(Philips 7724).

★Before replacing the bulb, make sure to cut off the

main power and wait for both the bulb base and

bulb cooling down.

2-2-2 Assemble the Light Source Group

Push the light source holder①into the body holder,

Make the light source group to be horizontal with the

body group and lock the screw②. (See Fig. 3)

2-2-3 Assemble the Stage

(1) Loosen the lock-screw①on the stage fully. (See

Fig. 4)

(2) From a rear area of the rounded hole center on the

base, carefully ring the two “V”buttons on the bottom

of the stage into the “V”rounded groove②, then screw

down the lock screw①.

Fig.3

Fig.2

Fig.1

Fig.4

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