Olympus CX21LED User manual

INSTRUCTIONS

CX21LED

BIOLOGICAL MICROSCOPE

A X 7 8 6 9

This instruction manual is for the Olympus Biological Microscopes Model CX21LED. To ensure the

safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope,

we recommend that you study this manual thoroughly before operating the microscope. Retain this

instruction manual in an easily accessible place near the work desk for future reference.

NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection

against harmful interference when the equipment is operated in a commercial environment. This

equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in

accordance with the instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference in which case

the user will be required to correct the interference at his own expense.

FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance

could void the user’s authority to operate the equipment.

This device complies with the requirements of directive 98/79/EC concerning in vitro diag-

nostic medical devices. CE marking means the conformity to the directive.

In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment,

this symbol indicates that the product must not be disposed of as unsorted municipal waste, but

should be collected separately.

Refer to your local Olympus distributor in EU for return and/or collection systems available in your

country.

CX21LED

CONTENTS

7-11

IMPORTANT — Be sure to read this section for safe use of the equipment. —

1 STANDARD COMPONENT UNITS

2 NOMENCLATURE

4 DETAILED OBSERVATION PROCEDURE

SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE

1-3

10-1 System Diagram of Optional Accessories .............................................................................. 18

10-2 Installation and Operation of Optional Accessories ................................................ 18

1Turning the LED Illumination ON .......... 7

3Adjusting the Focus ................................................ 8

5Adjusting the Diopter ............................................ 9

Placing Specimen on the Stage

..................... 7

Adjusting the Interpupillary Distance

.................... 9

5 ONE-POINT ADVICE

6 TROUBLESHOOTING GUIDE

7 SPECIFICATIONS

8 OPTICAL CHARACTERISTICS

9 ASSEMBLY

10 OPTIONAL ACCESSORIES

13,14

18-20

1Filter Holder CH2-FH and Darkfield Ring CH2-DS........................................................................................................ 18

2Reflection Mirror CH20-MM ........................................................................................................................................................................... 19

3Eyepieces WHC15X/WHC15X-H

........................................................................................................................................................................................................

4Using the eyepiece micrometer

6Adjusting the Condenser Position and Aperture Iris Diaphragm ............................................................. 10

7Switching the Objectives............................. 10 8

Using the 100X Immersion Objective ....................

PROPER SELECTION OF THE POWER SUPPLY CORD ......................................................... 21,22

IMPORTANT

SAFETY PRECAUTIONS

1. After the equipment has been used in an observation of a specimen that is accompanied with a potential of

infection, clean the parts coming in contact with the specimen to prevent infection.

· Moving this product is accompanied with the risk of dropping the specimen. Be sure to remove the specimen before

moving this product.

· In case the specimen is damaged by erroneous operation, promptly take the infection prevention measures.

2. Install the microscope on a sturdy, level table or bench.

3. Always use the AC adapter and power cord provided by Olympus. If no power cord is provided, please select the proper

power cord by referring to the section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruc-

tion manual. If the proper power cord is not used, product safety performance cannot be warranted.

4. Always ensure that the grounding terminal of the microscope and that of the wall outlet are properly connected. If the

equipment is not grounded, Olympus can no longer warrant the electrical safety performance of the equipment.

5. After operation or in case of abnormality, be sure to disconnect the power cord of the AC adapter from the connector on

the microscope or from the wall power outlet.

Safety Symbols

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment

in the safest possible manner.

Symbol Explanation

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

Before use, carefully read the instruction manual. Improper use could result in personal injury to

the user and/or damage to the equipment.

LED (light emitting diode) safety warning

The LED built in this product is classified as Class 1 LED Product and is basically eye-safe. However, direct viewing the

light from the LED unit for extended time may cause damage to the eyes.

Class 1 LED Product

CX21LED

1Getting Ready

1. A microscope is a precision instrument. Handle it with care and avoid

subjecting it to sudden or severe impact.

2. Do not use the microscope where it is subjected to direct sunlight,

high temperature and humidity, dust or vibrations. (For the operating

conditions, see chapter 7, “SPECIFICATIONS” on Page 15.)

3. Always use the tension adjustment ring to adjust the rotation tension of

the coarse adjustment knob.

4. Be sure to leave enough space around the AC adapter when installing

the microscope.

5. When carrying the microscope, first make sure that the AC adapter has

been disconnected and hold both sides around the hole of the arm as

shown in Fig. 1 and carry carefully.

# To prevent damage, do not hold the microscope by the stage 1or

observation tube 2.

Be sure to remove the specimen; otherwise, it may fall.

Fig. 1

2Maintenance and Storage

1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and wipe

gently using a piece of cleaning paper (or clean gauze).

If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute

alcohol.

Since the absolute alcohol is highly flammable, it must be handled carefully.

Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical

equipment that is being switched on or off, since such operation may cause ignition of a fire.

Also remember to always use it only in a well-ventilated room.

2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean

them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.

3. Do not disassemble any part of the microscope as this could result in malfunction or reduced performance.

4. When not using the microscope, store it in a dry locker or cover it with a dust cover.

Fig. 2

5. To clean the condenser, fully loosen the securing knob 1, then remove

the condenser by lowering it with the condenser height adjustment knob

2, and wipe the front lens of the condenser.

The condenser can be attached by reversing the above removal

procedure.

6. When disposing of the microscope. Check the regulations and rules

of your local government and be sure to observe them.

4 Caution

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,

the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

: Indicates that failure to follow the instructions in the warning could result in bodily harm to the

user and/or damage to equipment (including objects in the vicinity of the equipment).

# : Indicates that failure to follow the instructions could result in damage to equipment.

} : Indicates commentary (for ease of operation and maintenance).

3Intended use

This instrument has been designed to be used to observe magnified images of specimens in routine and research

applications.

Do not use this instrument for any purpose other than its intended use.

CX21LED

STANDARD COMPONENT UNITS

}After opening the package, make sure that the correct component units for the selected set are present.

· The 100X objective and immersion oil are provided only with the CX21LEDFS1 set.

# The objectives have been screwed in tightly to prevent them from being loosened during transportation. To

remove an objective, turn it counterclockwise while holding it with a rubber sheet, etc., so that your fingers don’t slip.

Eyepieces

(Fixed at 10X)

Stage

Condenser

Microscope frame

· 4X, 10X, 40X, 100X

(CX21LEDFS1)

· 4X, 10X, 40X

(CX21LEDFS2)

Immersion oil

(CX21LEDFS1)

Optional Accessories

· Filter Holder

CH2-FH

· Reflection Mirror

CH20-MM

· Eyepieces

WHC15X

WHC15X-H

· Darkfield Ring

CH2-DS

AC adapter

Power cord

Binocular observation

tube Objectives

Revolving nosepiece

“ ”: Power ON

“ ”: Power OFF

NOMENCLATURE

}The following items have been attached at the factory to prevent deterioration during transport. Remove these items and

retain them for future use. Then loosen the observation tube clamping knob and correct the orientation of the eyepieces

as shown in the illustration below.

1 Revolving nosepiece/observation tube transport band

2 Stage and specimen holder protection sheet

3 Protective pad below the stage

}Attach the AC adapter and power cord as described in chapter 9, “ASSEMBLY” on page 17.

Diopter adjustment ring

(Page 9)

}For detailed description of each control, refer to the page indicated inside parentheses.

Specimen holder (Page 7)

Aperture iris diaphragm ring

(Page 10)

Filter holder

Light intensity adjustment

knob (Page 7)

Revolving nosepiece

(Page 10)

Observation tube clamping

knob

Pre-focusing knob (Page 9)

Specimen holder Y-axis

feed knob (Page 7)

Specimen holder X-axis

feed knob (Page 7)

Fine adjustment

knob (Page 8)

Coarse adjustment

knob (Page 8)

Main switch (Page 7)

Accommodates a

45-mm diameter filter.

CX21LED

SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE

Set the main switch to “ I" (ON) and adjust

the brightness.

1Main switch (P. 7)

2Light intensity adjustment knob (P. 7)

Place the specimen on the stage. 3Specimen holder (P. 7)

4X-axis/Y-axis feed knobs (P. 7)

Engage the 10X objective in the light path. 5Revolving nosepiece (P. 10)

Bring the specimen in focus 6Coarse/fine focus adjustment knobs (P. 8)

Adjust the interpupillary distance.

Adjust the diopter.

7Binocular tube (P. 9)

8Diopter adjustment ring (P. 9)

Adjust the aperture iris diaphragm. 9Aperture iris diaphragm ring (P. 10)

Engage the objective to be used in the light

path and bring the specimen in focus.

aFilters (Diameter: 45 mm) (P. 5)

Engage the required filters.

5Revolving nosepiece (P. 10)

6Coarse/fine adjustment knobs (P. 8)

Adjust the brightness. 2Light intensity adjustment knob (P. 7)

Start observation.

(Controls Used) (Page)

Fig. 3

DETAILED OBSERVATION PROCEDURE

1Turning the LED Illumination ON (Fig. 3)

1. Set the main switch 1to “ I” (ON).

2. Rotating the light intensity adjustment knob 2in the direction of the

arrow increases brightness and rotating it in the opposite direction de-

creases brightness. The figures around the knob indicate the reference

brightness.

2Placing Specimen on the Stage (Figs. 4 & 5)

Fig. 4

# Place the specimen gently. If the bow-shaped lever is returned with

a strong force or the knob 1of the bow-shaped lever is released in

the middle, the slide glass may be broken.

1. Rotate the coarse adjustment knob 2in the direction of the arrow to fully

lower the stage.

2. Open the bow-shaped lever 3outward, place the specimen by sliding

the specimen glass plates on the stage from the front toward the rear.

3. After sliding the specimen glass plates all the way, return the bow-shaped

lever 3gently.

4. Rotating the upper knob which is the Y-axis feed knob 4moves the

specimen in the vertical direction. Rotating the lower knob which is the X-

axis feed knob 5moves it in the horizontal direction.

#Do not move the specimen holder directly by hand, for this will

damage the rotary mechanisms of the above knobs.

#When the specimen holder reaches the stopper position, the ro-

tation force of the above knobs become heavy. Stop rotating the

knob at this time.

Cover glass

This is the glass plate placed on the specimen. To allow the objective

manifest the full performance, the cover glass thickness, which is the

distance from its surface to the specimen surface, should ideally be

0.17 mm.

Slide glass

This glass plate should ideally have a length of 76 mm, width of 26

mm and thickness between 0.9 and 1.4 mm.

Fig. 5

Placing Specimen

Cover glass

Slide glass

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