Olympus SZX10 User manual

INSTRUCTIONS

SZX10

RESEARCH HIGH-CLASS STEREO

MICROSCOPE

This instruction manual is for the Olympus SZX10 Research High-Class Stereo Microscope System.

To ensure the safety, obtain optimum performance and to familiarize yourself full with the use of this

microscope, we recommend that you study this manual thoroughly before operating the microscope.

Retain this instruction manual in an easily accessible place near the work desk for future reference.

A X 7 4 8 0

SZX10

SZX-EPA

SZX-R/SZH-P400/SZH-P600

SZX2-2RE10

SZX-STAD1

SZX2-AN

SZX-PO

SZX-STAD2

SZH-STAD1

Modules described in this manual

This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic

medical devices. CE marking means the conformity to the directive.

SZX10

CONTENTS

IMPORTANT — Be sure to read this section for safe use of the equipment. —

1 NOMENCLATURE

2 CONTROLS

3 SUMMARY OF OBSERVATION PROCEDURE

4 USING THE CONTROLS

4-1 Base ........................................................................................................................................................................................................................... 7

4-2 Microscope Body and Focusing Assembly................................................................................................ 7, 8

4-3 Observation Tube ......................................................................................................................................................................... 9-11

4-4 TV Observation and Photomicrography ............................................................................................................ 12

1Using the Stage Plate 2Placing the Specimen

1-3

5 TROUBLESHOOTING GUIDE 13

7-12

1Adjusting the Rotation Tension of the Coarse Focus Adjustment Knob

2 Engaging and Disengaging the Zooming Knob Click Stop Function

3Adjusting the Aperture Iris Diaphragm 4Zoom Magnification Indication

1Adjusting the Interpupillary Distance

2Adjusting the Diopter (Zoom Parfocal Adjustment)

3Using the Eye Shades 4Using the Eyepiece Micrometer Disk

5Selecting the Light Path of the Trinocular Tube

6 Adjusting the Tilt

1Selecting the TV Adapter Magnification 2Mounting the TV Adapter

3Selecting the TV Camera Light Path

6 SPECIFICATIONS 14,15

OBSERVATION MAGNIFICATIONS AND OBSERVATION AREAS

8 ASSEMBLY 17-20

9 OPERATION OF OTHER MODULES 21-30

9-1 Eyepoint Adjuster SZX-EPA .............................................................................................................................................. 21

9-2 Drop Prevention Collar SZX-R and

Auxiliary Pillar SZH-P400/SZH-P600 ................................................................................................................... 22

9-3 Revolving Nosepiece SZX2-2RE10............................................................................................................ 23-25

9-4 BX Stage Adapter Type 1 SZX-STAD1 ................................................................................................... 26-28

9-5 BX Stage Adapter Type 2 SZX-STAD2 .................................................................................................... 29,30

9-6 Stage Adapter Type 1 SZH-STAD1......................................................................................................................... 30

SZX10

IMPORTANT

SAFETY PRECAUTIONS

1. After the equipment has been used in an observation of a specimen that is accompanied with a potential of infection,

clean the parts coming in contact with the specimen to prevent infection.

· Moving this microscope is accompanied with the risk of dropping the specimen and causing infections. Be sure to

remove the specimen before moving this product.

· In case the specimen is damaged by erroneous operation, promptly take the infection prevention measures.

· The microscope may become unstable when certain intermediate attachments and/or photography unit are mounted

on it. Take the measures so that the microscope does not turn over.

2. To prevent the microscope body from turning over, its left-right pivot

angle must be limited to 30° as shown in Fig. 1. The tilt of the desktop

surface should be no more than 5°.

3. Sufficient care is required during observation with a low-magnification

objective, because the objective’s long WD (Working Distance) can dis-

place the microscope body to a higher position.

The measures for prevention of turning over are also required when us-

ing an auxiliary pillar (SZH-P400/P600), because this also makes the

microscope body to be displaced to a higher position.

4. To adjust the microscope body height, be sure to hold the microscope

body with one hand while loosening the focusing assembly clamping

knob @. (Fig. 1)

(Use the drop prevention collar (SZX-R) to prevent a hazard from occur-

ring.)

Be careful not to pinch your finger during the adjustment.

Fig. 1

1Getting Ready

1. A microscope is a precision instrument. Handle it with care and avoid

subjecting it to sudden or severe impact.

2. Do not use the microscope where it is subjected to direct sunlight,

high temperature and humidity, or vibration. (For the operating envi-

ronment, see Chapter 6, “SPECIFICATIONS” on page 15.)

3. When moving the microscope, remove the specimen in advance. Then,

hold the front part of the base with one hand and hold the rear part of

the base with the other hand to avoid tilting the microscope.

Also remove any module or attachment from the microscope to mini-

mize the weight before moving.

4. Observe the following cautions when operating the coarse focus adjust-

ment knob or the zooming knob.

Operation

Manipulated

Controls Caution

Focusing Coarse/fine focus

adjustment knobs

@ (Fig. 3)

1. If the knob hits the upper or lower limit-

ing mechanism violently or it is rotated

after it hits a limiting mechanism, the

internal mechanism may be damaged.

2. If the knobs on the left and right are ro-

tated in opposite directions, the internal

mechanism will be damaged. (The ro-

tation tension of the coarse focus ad-

justment knob should be adjusted us-

ing the rotation tension adjustment ring

³ on the knob.)

Zooming Zooming knob ²

(Fig. 3)

1. If the knob hits the upper or lower limit-

ing mechanism violently or it is rotated

after it hits a limiting mechanism, the

internal mechanism may be damaged.

2. If the knobs on the left and right are

rotated in opposite directions, the in-

ternal mechanism will be damaged.

5. Only one intermediate attachment can basically be used, but two

attachments may also be used though the peripheral part of the

image may be obscured.

However, the coaxial vertical illuminator (SZX2-ILLC10) is not consid-

ered to be an intermediate attachment.

}When more than one intermediate attachment is to be combined, they

should be stacked according to the following order, from the bottom to

the top: SZX2-ILLC10, SZX-RFA, SZX-SDO, SZX-DO, SZX2-LBS, SZX-DA,

SZX-FAD, SZX-EPA.

Fig. 2

Fig. 3

²³

SZX10

2Maintenance and Storage

1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and

wipe gently using a piece of cleaning paper (or clean gauze).

If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute

alcohol.

Since the absolute alcohol is highly flammable, it must be handled carefully.

Be sure to keep it away from open flames or potential sources of electrical sparks --- for example, electrical

equipment that is being switched on or off.

Also remember to always use it only in a well-ventilated room.

2. The equipment uses plastic resins extensively in its external finish. Do not attempt to use organic solvents to clean them

but simply wipe them using a lint-free, soft cloth. To clean an extremely dirty part, use a soft cloth lightly moistened with a

diluted neutral detergent.

3. Never disassemble any part of the microscope as this could result in malfunctions or reduced performance.

4. When not using the microscope, keep it covered with the dust cover provided. If a module containing a source of heat is

mounted on the microscope, wait until it has cooled down before covering.

5. When disposing of the microscope. Check the regulations and rules of your local government and be sure to

observe them.

3Caution

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,

the microscope may also be damaged. Always use the microscope as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

: Indicates that failure to follow the instructions in the warning could result in bodily harm to the

user and/or damage to equipment (including objects in the vicinity of the equipment).

# : Indicates that failure to follow the instructions could result in damage to equipment.

} : Indicates commentary (for ease of operation and maintenance).

4Intended use

This instrument has been designed to be used to observe magnified images of specimens in routine and research

applications.

Do not use this instrument for any purpose other than its intended use.

}The following illustration shows a typical system composed of modules marked with “{” in the list of each module, and

other modules may also be used in place of them. For the modules that are not shown in the module lists below, please

contact Olympus or the latest catalogues.

*A separate instruction manual is available for the module marked *.

NOMENCLATURE

Eyepieces

{WHS10X-H

· WHSZ15X-H

· WHSZ20X-H

· WHSZ30X-H

Stage plate

{Monochrome plate: SZ2-SPBW/SP-BW2

· Glass plate: SP-C

· Fluorescence center plate: SP-FL

Microscope

body

{10X zoom microscope body:

SZX2-ZB10

Objective

· DFPL0.5X-4**

· DFPL0.75X-4

{DFPLAPO1X-4

· SZX-ACH1X

· DFPLAPO1.25X

· SZX-ACH1.25X

· DFPL1.5X-4

· DFPL2X-4

Observation tube

· Binocular observation tube:

SZX-BI30/BI45

{Trinocular observation tube:

SZX2-TR30/TR30PT

· Tilting binocular tube:

SZX-TBI

· Tilting trinocular observation tube:

SZX2-TTR/TTRPT

Focusing assembly

· Coarse/fine focusing assembly:

SZX2-FOF

· Focusing assembly: SZX2-FO

{Coarse/fine focusing assembly

for heavy load: SZX2-FOFH

Intermediate attachments

· Eyepoint adjuster: SZX-EPA

· Coaxial vertical illuminator:

SZX2-ILLC10

· Discussion observation tube: SZX-DO

· Vertical fluorescent light illuminator:

SZX-RFA

· Other

Base

{Standard base: SZX2-ST**

· Large base: SZX2-STL

· Transmitted light base: SZX2-ILLB*

SZX2-ILLD*

SZX2-ILLK*

SZX2-ILLT*

** The standard base (SZX2-ST) requires use of the optional auxiliary pillar (SZH-P400) and optional drop prevention collar

(SZX-R). The large base (SZX2-STL) comes with the auxiliary pillar (SZH-P400) mounted as standard. This enables the

large base to be used as is. However, be sure to use the drop prevention collar (SZX-R) in combination.

SZX10

CONTROLS

}If the microscope is not yet assembled, see Chapter 8, “ASSEMBLY” (pages 17 to 20) before the following.

Diopter adjustment ring (P. 9)

±5 m-1 (per meter)

Eyepiece clamping knob

Aperture iris diaphragm ring (P. 8)

Zoom magnification indication (P. 8)

0.63 to 6.3 (11 indications)

Click stop ON-OFF screw (P. 8)

Engages or disengages the click

stop function for each zoom mag-

nification.

Light path selector knob (P. 11)

Trinocular tube only.

Zooming knob

0.63X to 6.3X

Coarse focus adjustment knob

rotation tension adjustment ring

(P. 7)

Fine focus adjustment knob

(SZX2-FOF/FOFH)

Stroke: 80 mm

Stroke per turn: 0.77 mm

Coarse focus adjustment knob

Total stroke: 80 mm

Stroke per turn (SZX2-FOF/FOFH): 36.8 mm

Stroke per turn (SZX2-FO): 21 mm

Specimen holder

Locks the specimen.

Focusing assembly

clamping knob

SUMMARY OF OBSERVATION PROCEDURE

Ref. Page

1. Check and tighten the connection of each component, especially the observation tube. ............................................ (Page 19)

2. Check that the angled formed by the microscope body with respect to the base is less

than the turning-over prevention angle.................................................................................................................................................................................................(Page 18)

3. Adjust the rotation tension of the coarse focus adjustment knob....................................................................................................................... (Page 7)

4. Confirm the correct settings.

3-1 Preparation

3-2 Observation Procedure

@²

4. Set the zooming knob @ to the lowest zoom magnification and bring the

microscope into focus by rotating the coarse focus adjustment knob ².

5. Rotate the zooming knob @ to the desired magnification and precisely

focus the microscope on the specimen with the coarse focus adjust-

ment knob ² and fine focus adjustment knob ³ (the fine focus adjust-

ment knob is not provided with the SZX2-FO).

}The contrast of the observed image and the focal depth of the specimen

can be adjusted with the aperture iris diaphragm ring.

1. Place a specimen on the stage. (Page 7)

2. Adjust the interpupillary distance. (Page 9)

3. Make diopter adjustment of the eyepieces. (Page 9)

(The adjustment procedure is variable depending on whether the eye-

piece micrometer disk is used or not.)

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