Olympus Fluoview FV1000 User manual

User’s Manual

[Quick Start]

FLUOVIEW

FV1000

LASER SCANNING

BIOLOGICAL MICROSCOPE

FV10-ASW [Ver2.0]

Notice

Thank you for your purchase of Olympus microscope at this time.

Hold this manual by your side when using this microscope all the time and keep it with care after reading. AX7797

Caution

FV1000MPE is a CLASS 4 laser product; FV1000 is a CLASS 3B laser product.

The procedures for using this system are classified as follows:

zService

“Service” means any adjustment or repair performed by highly trained and skilled technical personnels who

are provided the service training following to the service manual for this system.

The performance has influence on the feature of this system, and there is a risk which unintended CLASS

3B or CLASS 4 laser light is emitted.

zMaintenance

“Maintenance” means adjustment or other procedures performed by customers to maintain that this system

functions properly.

zOperation

“Operation” means all performance described in the user’s manuals in this system.

CLASS 3B or CLASS 4 laser light is only emitted from the objective lens during the actual execution.

The User’s Manuals of this system consist of the following:

In order to maintain the full performance of this system and ensure your safety, be sure to read these user’s

manuals and the operating instructions for the laser unit and light source unit before use.

User’s manual constitution of FV1000MPE

zFV1000MPE / FV1000 User’s Manual [Laser Safety Guide]

zFV1000MPE User’s Manual [Safety Manual] or [Safety Guide]

zFV1000 User’s Manual [Safety Guide]

zFV1000MPE User’s Manual [Operation Manual] or [Operation]

zFV1000 User’s Manual [Hardware Manual]

zFV1000 FV10-ASW User’s Manual [Quick Start]

User’s manual constitution of FV1000

zFV1000MPE / FV1000 User’s Manual [Laser Safety Guide]

zFV1000 User’s Manual [Safety Guide]

zFV1000 User’s Manual [Hardware Manual]

zFV1000 FV10-ASW User’s Manual [Quick Start]

Also, we have prepared one service manual for this system as below. Technical personnels who perform the

service require to take the service training.

zFV1000MPE / FV1000 Service Manual

In case of purchasing the laser simultaneously, we have prepared the following manual for the laser.

zMaiTai Series User’s Manual [Quick Start]

In addition, we have prepared one service manual for the laser as below. Technical personnels who perform the

laser service require to take the service training.

zMaiTai Series Service Manual

Part or whole of this software as well as manual shall not be used or duplicated without consent.

Registred trademark

Microsoft, Microsoft Windows are registered trademarks of Microsoft Corporation.

Other brand names and product names are trademarks or registered trademarks of their respective owners.

CONTENTS

This Quick Start has divided into the volume by the following system configurations.

IX81 (Spectral Type)

IX81 (Filter Type)

BX61 (Spectral Type)

BX61 (Filter Type)

Laser Conforcal Scanning

Microscope

FV1000D Spectral Type

(invertedMicroscpeIX81)

Operation Manual

Contents

System introduction 3

FV1000D Laser DyeList 4

System Preparation 5

Visible Observation

Observation of Fluorescence image 6

Observation of Differential Interference Contrast Images 7

Image Acquisition

Overview of Operation Panel for Image Acquisition 8

Single Stain on XY Image 9-11

Complement of adjusting the image 12-13

Double Stain on XY Image 14-15

Sequential scan Line Sequential 16

Double Stain on XYZ image 17-18

Four Stain on XY Image 19-21

Single Stain + DIC on XY Image 22-23

Merge the image between fluorescent XY image and DIC image 24

Single Stain on XYZT Image / ZDC XYZT image 25-26

Spectral Image on XYZ Image 27-29

Unmixing 30-33

Reload the image conditions 34

Image Analysis

Overview of the 2D Operation Panel / Opening a file 35

Making 2D Z projection file Images 36

Saving a Z section image as 2D image 37

Inserting the Scale Bar 38

Rotating a Three-dimensional Image 39

Saving an Image 40

Saving Rotating a Three-dimensional animation file 41

2D Image Analysis

Edit the image color and contrast 42

The image of Z section 43

Intensity Profile of each Z sections 44

Measure 45

Line Intensity Profile on the 2D image / Histogram 46

Line Series Analysis / Co-localization 47

TimeLapse Analysis 48

Closing the System 48

Spectral Type Main Scanner

Dye List (FV1000D Lasers are available below)

LD405nm LD440nm LD473nm LD559nm LD635nm

Ar458nm Ar488nm Ar515nm

HeNe(G)543nm

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