Olympus CX31 User manual

CX31

INSTRUCTIONS

BIOLOGICAL MICROSCOPE

AX6654

This instruction manual is for the Olympus Biological Microscope Model CX31. To ensure

the safety, obtain optimum performance and to familiarize yourself fully with the use of this

microscope, we recommend that you study this manual thoroughly before operating the

microscope. Retain this instruction manual in an easily accessible place near the work

desk for future reference.

CX31

CONTENTS

IMPORTANT — Be sure to read this section for safe use of the equipment. — 1-3

1 NOMENCLATURE 4

SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE

3 ASSEMBLY — See this section for the replacement of the light bulb. — 6-7

4 USING THE CONTROLS 8-14

5 TROUBLESHOOTING GUIDE 15-16

7 OPTICAL CHARACTERISTICS 18

4-1 Base..........................................................................................................................................................................................8

Turning On the Bulb Field Iris Diaphragm

4-2 Focusing Block...............................................................................................................................................................9

Adjusting the Coarse Adjustment Knob Tension Simplified Pre-focusing Dial

4-3 Stage..............................................................................................................................................................................10-11

Placing the Specimen Moving the Specimen

4-4 Observation Tube................................................................................................................................................11-12

Adjusting the Interpupillar Distance Adjusting the Tilt Adjusting the Diopter

Using the Eye Shades Using the Eyepiece Micrometer Disk

4-5 Condenser........................................................................................................................................................................13

Centering the Field Iris Diaphragm Aperture Iris Diaphragm

4-6 Immersion Objectives............................................................................................................................................14

Using the Immersion Objectives

PROPER SELECTION OF THE POWER SUPPLY CORD..................................................19-20

6 SPECIFICATIONS 17

Page

1 2

4 5

Fig.1

1. After the equipment has been used in an observation of a specimen

that is accompanied with a potential of infection, clean the parts

coming in contact with the specimen to prevent infection.

· Moving this product is accompanied with the risk of dropping the

specimen. Be sure to remove the specimen before moving this

product.

· In case the specimen is damaged by erroneous operation, promptly

take the infection prevention measures.

2. To avoid potential shock hazard and fire, always set the main switch @

to “ ” (OFF) and disconnect the power cord from the AC receptacle

at the rear of the microscope and from the wall outlet before replacing

the bulb. Allow the lamp housing cover ² and the bulb to cool before

touching them.

3. Install the microscope on a sturdy, level table.

The air vents on the underside of the base should never be blocked

by placing the microscope on a flexible surface such as a carpet, as

this could result in overheating and cause a fire.

4. Always use the power cord provided by Olympus. If no power cord is

provided, please select the proper power cord by referring to the

section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the

end of this instruction manual. If the proper power cord is not used,

Olympus can no longer warrant the electrical safety and performance

of the equipment.

5. When installing the microscope, route the power cord away from the

microscope base. Should the power cord come in contact with the hot

microscope base, the power cord could melt and cause electric shock.

6. Connect the power cord correctly and ensure that the grounding

terminal of the power supply and that of the wall outlet are properly

connected. If the equipment is not grounded, Olympus can no longer

warrant the electrical safety and performance of the equipment.

7. Never set the main switch @ to “ ” (ON) while any metallic object is

inserted into the air vents of the microscope frame as this will result in

electrical shock, personal injury and equipment damage.

8. When the microscope is not in use or when it is malfunctioning,

disconnect the power cord plug from the AC receptacle or from the

wall outlet.

IMPORTANT

SAFETY PRECAUTIONS

CX31

A warning label is affixed at parts where special precaution is required when handling and using the microscope. Always

heed the warnings.

Fig. 2

Indicates that the surface becomes hot, and should not be touched with bare hands.

Before use, carefully read the instruction manual. Improper handling could result in personal

injury to the user and/or damage to the equipment.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

ExplanationSymbol

1. A microscope is a precision instrument. Handle it with care and avoid

subjecting it to sudden or severe impact.

2. Do not use the microscope where it is subjected to direct sunlight,

high temperature and humidity, dust or vibrations. (For the operating

environment conditions, refer to and adhere to the conditions specified

in Chapter 6, “SPECIFICATIONS” on page 17.)

3. The tension of the coarse focus adjustment knob should only be

adjusted by means of the tension adjustment ring.

4. Heat from the microscope is led away by natural convection.

Consequently, leave an enough space (10 cm or more) on the rear of

the microscope and ensure that the room is well ventilated.

5. When moving the microscope, carefully carry it with one hand under

the base @ and the other hand holding at the recessed handle on the

rear of the arm ² as shown in the illustration on the left.

#Damage to the microscope will occur if you hold it by the stage, X-

axis/Y-axis knob, binocular section of the observation tube, etc.

Also be sure to remove the specimen to prevent it from falling off.

#Sliding the microscope on the surface of the table may damage or

tear off the rubber feet and/or scratch the table top surface.

Getting Ready

Safety Symbols

Warning Label

The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment in

the safest possible manner.

If the warning label becomes soiled, peeled off, etc., contact Olympus to have it replaced.

Base underside

(Caution for bulb replacement)

Warning label

position:

1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze

slightly moistened with a mixture of ether (70%) and alcohol (30%).

Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to

keep these chemicals away from open flames or potential sources of electrical sparks -- for example,

electrical equipment that is being switched on or off. Also remember to always use these chemicals only in a

well-ventilated room.

2. Do not attempt to use organic solvents to clean the non-optical components of the microscope. To clean them, use a

lint-free, soft cloth lightly moistened with a diluted neutral detergent.

3. Do not disassemble any part of the microscope as malfunction or damage may occur.

4. When not using the microscope, ensure that the frame is cooled down and store it in a locker or cover it with a dust

cover.

5. When disposing of the microscope, check the regulations and rules of your local goverment and be sure to observe

them.

Maintenance and Storage

If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In

addition, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.

The following symbols are used to set off text in this instruction manual.

: Indicates that failure to follow the instructions in the warning could result in bodily harm to the

user and/or damage to equipment (including objects in the vicinity of the equipment).

# : Indicates that failure to follow the instructions could result in damage to equipment.

} : Indicates commentary (for ease of operation and maintenance).

Caution

This device complies with the requirements of directive 98/79/EC concerning in vitro diagnostic medical devices.

CE marking means the conformity to the directive.

NOTE : This equipment has been tested and found to comply with the limits for a Class A digital device,

pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection

against harmful interference when the equipment is operated in a commercial environment. This

equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in

accordance with the instruction manual, may cause harmful interference to radio communications.

Operation of this equipment in a residential area is likely to cause harmful interference in which case the

user will be required to correct the interference at his own expense.

FCC WARNING : Changes or modifications not expressly approved by the party responsible for compliance

could void the user's authority to operate the equipment.

CX31

NOMENCLATURE

}The revolving nosepiece is fastened with a band to prevent it from turning during transportation. Remove the band when

unpacking the microscope. Be sure to store the band for re-transportation of the microscope.

* The stage is shipped with the two transport pins locked. When using the microscope for the first time, remove the transport

lock pins before use.

}If you have not yet attached the lamp bulb and power cord to the microscope, read Chapter 3, “ASSEMBLY” on pages 6 to 7.

Specimen holder

(Page 10)

Condenser

Aperture iris

diaphragm knob

(Page 13)

Filter holder

Place a 45 mm filter on this.

Condenser

centering screws

(Page 13)

Field iris diaphragm ring

Objective

Revolving Nosepiece

Diopter

adjustment ring

(Page 11)

Transport lock pin*

Observation Tube

Binocular tube: CX31RBSF microscope frame

CX31RBSFA microscope frame

Tilting tube: CX31RTBISF microscope frame

Microscope Frame

CX31RBSF

CX31RBSFA

CX31RTBISF

Stage

Interpupillar distance scale (Page 11)

Tube clamping screw

(Use the provided Allen wrench.)

Simplified pre-focusing dial (Page 9)

Transport lock pin*

Main switch

(Page 8)

Light intensity

control knob

(Page 8)

Fine focus

adjustment knob

(Page 9)

Coarse focus

adjustment knob

(Page 9)

X-axis knob (Page 11)

Y-axis knob (Page 11)

(Fixed at 10X)

Eyepieces

SUMMARY OF BRIGHTFIELD OBSERVATION

PROCEDURE

123

456

789

· Turn the revolving nosepiece to

engage the 10X objective.

#Make sure that the revolving

nosepiece stops with an audible

click.

· Place a specimen on the stage.

(Page 10)

· Turn the X-axis knob and Y-axis

knob to move the specimen into

the light path. (Page 11)

· Set the main switch to “ I” (ON)

and adjust the brightness with the

light intensity knob. (Page 8)

· Turn the coarse and fine

adjustment knobs to bring the

specimen into focus.

· Adjust the interpupillary distance.

(Page 11)

· When the tilting observation tube is

used, adjust the angle of tilt.

(Page 11)

· Adjust the diopter. (Page 11) · Center the field iris diaphragm.

(Page 13)

· Adjust the aperture iris diaphragm

and field iris diaphragm. (Page 8)

10 Engage the objective to be used for observation in the light path, then readjust the focus.

11 Place the required filter on the filter holder.

12 Re-adjust the aperture iris diaphragm, field iris diaphragm and brightness and start observation.

CX31

ASSEMBLY

1. Turn the microscope frame on its side and pull the lamp housing

knob @ on the underside of the base to open the lamp housing cover.

2. Holding the halogen bulb ² contained in the polyethylene bag to

avoid leaving fingerprints on the bulb, fully insert the contact pins into

the bulb socket ³ When properly seated, pull off the polyethylene bag.

6 V, 30 W halogen bulb: 6V30WHAL (Philips 5761)

Always use the designated bulb. Use of improper bulb may result

in fire.

Do not touch the bulb with bare hands. If fingerprints are

accidentally left on the bulb, wipe the bulb with a soft, lint-free

cloth moistened with alcohol. Using a contaminated bulb will

shorten the service life of the bulb.

3. With the lamp housing knob still pulled out, close the lamp housing

cover. Then push in the lamp housing knob to lock the cover.

#The lamp housing cover cannot be closed if the knob is pushed in

before you attempt to close the cover.

Caution for Bulb Replacement During Use or Right After Use

Whenever you replace the bulb during use or right after use, first

move the main switch to “ ” (OFF), disconnect the power cord

from the wall outlet, and allow the bulb and parts around the bulb

to cool before touching.

#If the bulb burns out during an observation and needs to be

replaced, remove the specimen, filter and other objects likely to

fall off, before tilting the microscope frame to replace the bulb.

Mounting the Bulb (Replacement of Bulb) (Fig. 3)

Cables and cords are vulnerable when bent or twisted. Never

subject them to excessive force.

Make sure that the main switch @ is set to “ ” (OFF) before

connecting the power cord. (Fig. 4)

Always use the power cord provided by Olympus. IF no power

cord is provided with the microscope, please select the proper

power cord by referring to section “ROPER SELECTION OF THE

POWER SUPPLY CORD” at the end of this instruction manual.

1. Connect the power cord plug ² to the AC receptacle |. (Fig. 5)

Connect the power cord to a grounded, 3-conductor power outlet

and ensure that the ground terminal of the power supply and that

of the wall outlet are properly connected. If the equipment is not

grounded, Olympus can no longer warrant the electrical safety

performance of the equipment.

2. Plug the power cord plug | into the wall outlet ƒ. (Fig. 6)

Connecting the Power Cord (Figs. 4 - 6)

Fig. 6

Fig. 3

Fig. 5

Fig. 4

}By attaching the optional CH3-CH cord hanger on the rear of the

microscope frame, the power cord can be accommodated by winding

around the hanger.

Insert the hook ² of the cord hanger @ into the air vent groove on the

rear of the microscope by aligning the attaching position ³, and

clamp by sliding the cord hanger downward while pushing it against

the microscope with a strong force.

#Do not hold the microscope frame by the cord hanger when

carrying the microscope. Otherwise, the cord hanger may be

detached during transport, resulting in falling of the microscope

and personal injury.

Removal

To prevent electric shock, disconnect the power cord first. Also

make sure you use the provided Allen wrench, not a thin Allen

wrench.

Move the microscope frame to the edge of the table |, apply an

Allen wrench ƒ onto the lower part of the cord hanger @, and

move the entire hanger upward by pushing the Allen wrench

toward the directions 1 and 2 to remove. (Fig. 8)

Attaching the Optional Cord Hanger (CH3-CH)

(Figs. 7 & 8)

Fig. 7

Fig. 8

CX31

USING THE CONTROLS

4-1 Base

1. Set the main switch @ to “ ” (ON).

2. Turn the light intensity knob ² clockwise in the direction of the arrow

to make the illumination brighter or counterclockwise to make it darker.

The numbers around the knob indicates the reference voltage values.

Turning On the Bulb (Fig. 9)

Fig. 9

Using the field iris diaphragm ring @, adjust the diameter of the field

iris for objective power to the extent that it just circumscribes the field

of view. When the field iris diaphragm is stopped down to circumscribe

the field of view, it will exclude extraneous light and improve image

contrast within the field of view.

#When using the 100X objective, the field iris diaphragm image will

not be visible within the field of view. Accordingly, stop down the

diaphragm to its smallest diameter.

Field Iris Diaphragm (Fig. 10)

Fig. 10

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