Olympus FHT-521 User manual

OLYMPUS

RESEARCH

MICROSCOPES

INSTRUCTION

MANUAL

MODELS

FHT&EHTWITH§W:

INSTRUCTION

MANUAL

OLYMPUS RESEARCH MICROSCOPES

MODELS

FHT&EHT:~~:~~

TABLE OF CONTENTS

STANDARD

EQUIPMENT " 2

II.

SPECiFiCATiONS 4

Ill.

IDENTIFICATION

VARIOUS

COMPONENTS 5

IV.

DESCRIPTION OF EACH COMPONENT 6

V. OPERATING THE MICROSCOPE

VI.

OPTICAL

CHARACTERiSTICS

...............•......

·19

STANDARD

EQUIPMENT

Before assembly,

please

check

your

standard

outfit,

which consists

the following items:

Model

FHT

, ) Binocular Tube Versions

Con- Total

Model No. Eyepieces Objectives Stage denser magni-

fication

Bi P7x, Bi WF lOx, Ach

4x,Ach

lOx,

N.A.

28x-

FHT-521 Bi P15x, paired Ach 40x. 1.25 1500x

Ach

l00x

(oill

Bi P7x, Bi High-Eye- Ach

4x.Ach

lOx,

FrS

N.A.

28x-

FHT-522

pointWF

10x,Bi

P15x, FI

40x,

100x ISquare 1.40 2000x

K20x.

paired

loill

mechani·

Bi P7x, Bi High-Eye- Plan 4x. Plan

lOx,

cal stage

with left

N.A.

28x-

FHT-523

pointWF

lOx.

BiP15x. Plan

40x,

&right 1.40 2000x

K20x,

paired Plan

loox

loill

hand

coaxial

N.A.

Plan4x,Plan

lOx.

controls) 0.85

FHT-523- Bi SW10x, paired Plan

20x,

Plan40x with

40x-

SW SW

Plan

looxloill

aux. con

l000x

denser

lens

Trinocular Tube Versions

Con- Total

Model No. Eyepieces Objectives Stage

denser

magni-

fication

Bi P7x, BiWF10x,

Bi P15x. paired

Ach

4x,

Ach

lOx,

N.A.

28x-

FHT-531 Photo eyepieces:

Ach

40x,

1.25 1500x

FK2.5x,

FK3.3x

Ach 100x

(oill

FK5x,FK6.7x,

leach

Bi P7x, Bi High-Eye- FrS

point

WF10x,

Bi P15x,

Ach4x,Ach

lOx, (Square

FHT-532 Bi

K20x,

paired

FI40x,Fll00x

mechani-

N.A.

28x-

Photo

eyepieces:

loill

cal stage 1.40

2000x

FK2.5x,

FK3.3x.

with

left

FK5x,FK6.7x,l

each &right

Bi P7x, Bi High-Eye- hand

coaxial

point

lOx,

Bi P15x, Plan4x, Plan lOx. controls)

FHT-533 Bi

K20x.

paired Plan

40x,

N.A.

28x-

Photo

eyepieces: Plan

l00x

loill

1.40 2000x

FK2.5x,

FK3.3x,

FK5x,FK6.7x,l

each

N.A.

SW10x paired

Plan4x,Planl0x,

0.85

FHT-533-

Photo

eyepieces: Plan20x.Plan40x,

with

40x-

FK2.5x,

FK3.3x,

SWPlan

looxloill

aux. con·

looox

FK5x,FK6.7x,l

each

denser

lens.

Other items supplied

with

each

version:

Spare Bulbs,6V.5A,

TB-'

_. _.

2pcs.

Filter(blue) ..... ..................

pc.

Filter

Mount

(provided

only

with

achromatic/aplanatic condenser) 1pc.

Wooden Carrying Case. . 1

pc.

Certificate . . . . . 1

pc.

2. Model

EHT

, ) Binocular Tube Version

Can· Total

Model No. Eyepieces Objectives Stage denser magni-

fication

Bi P7x, Bi

WF10x.

Ach4x,

Ach

lOx.

N.A.

28x-

EHT

-421

Ach

40x.

P15x, paired Ach

loox

(oill

1.25 1500x

CrS·VH

Bi P7x,

High·Eye·

Ach4x.

Ach

lOx

ISquare N.A.

28x-

EHT-422

pointWFl

Ox.

5x. FI

40x.

loox

mechani- 1.40 2000x

K20x.

paired

loill

cal

stage

Bi P7x, Bi High·Eye- Plan

4x.

Plan

lOx.

with

co-

axial

low

N.A.

28x-

EHT

-423

pointWF

1Ox.Bi

5x. Plan

40x.

drive 1.40 2000x

K20x.

paired Plan

loox

loill

controls) N.A.

Plan

4x.

Plan

lOx.

0.85

EHT

-423· with

40x-

Bi SW10x. paired Plan20x.Plan40x, aux.

con·

looox

SWPlan

looxloill

denser

lens

Trinocular Tube Version

Con- Total

Model No. Eyepieces Objectives Stage denser magni·

fication

Bi P7x. Bi WF

lOx,

Bi P15x. paired. Ach

4x.

Ach

lOx,

N.A.

28x-

EHT

-431 Photo eyepieces :

Ach40x,Achloox

1.25 1500x

FK2.5x,FK3.3x,

(oill

FK5x,FK6.7x,1

each

Bi P7x. Bi High·Eye-

pointWF10x,

Bi P15x,

Ach4x,Ach

lOx,

CrS·VH

EHT-432 Bi

K20x,

paired. FI

40x.

loox

(Square

N.A.

28x-

Photo eyepieces:

(oill

mechani- 1.40

2000x

FK2.5x,

FK3.3x,

cal

stage

FK5x,

FK6.7x,

1each

with

low

Bi P7x, Bi High·Eye- drive

controls)

pointWF10x,

5x, Plan

4x,

Plan

lOx,

EHT-433

K20x,

paired. Plan

40x,

N.A.

28x-

Photo

eyepieces :Plan

loox

(oil)

1.40

2000-

FK2.5x, FK3.3x,

FK5x,

FK6.7x,l

each

N.A.

lOx,

paired. Plan4x, Plan

lOx,

0.85

EHT·433·

Photo

eyepieces: Plan

20x

,Plan40x,

with

40x-

FK2.5x,

FK3.3x

SWPlan

lOOx(oill

aux. con- 1000x

FK5x,

FK6.7x,l

each

denser

lens

Other items supplied

with

each

version:

Spare

Bulbs,6V,5A.TB·'

2pcs.

Filler(blue) ....................................

pc.

Filter Mount(provided

only

wilh

achromatic/aplanalic condenser) 1

pc.

Wooden Carrying Case. 1pc.

Certificate . . . . . . . . 1

pc.

SPECIFICATIONS

--------

FHT

EHT

Vertical stage movement. Vertical stage

movement.

Focusing coax

ial

coarse and fine ad· separate coarse

and

fine

justments, with

automatic

adjustments.

with

auto-

pre·focusing lever. matic pre-focusing lever.

Coarse

Devetait slideways, rack

and

pinion

type:

adjustment adjustment

range

32.5

Fine Adjustment ragne

1.2mm.

Adjustment

range

1.2mm,

adjustment graduated in increments

graduated in increments

Micra-

micron.

2microns.

scope

Tungsten lamp,

6V.

5A,

with

centering device and

built·in

Stand

Light transformer.

source

Auxiliary

lenses

for

low,

medium

and

high

power

objectives.

Revolving

Quintuple,

on ball bearings. Engravings. coded Ato E.

nosepiece to facilitate objective insertion.

Condenser

With

condenser centering device and rack·and-pinion

mount

height adjustment. Height displacement 23.5mm.

Tube

inclination

45-,

rotatable

360-,

both

eyepiece tubes

Binocular provided

with

diopter

and tube length adjustments,

tube except Bi-SW observation tube inclined

30-,

and

diopter

Obser-

difference

can

adjusted

the

eyepiece

tube.

vation I

nterpupillary

distance adjustment

from

56mm

74mm.

Tubes

Tube

inclination

45·,

rotatable

360·,

both

eyepiece tubes

provided

with

diopter

and tube length adjustments, except

Trinocular

Tr-SWobservation

tube inclined

30·,

and

diopter

difference

tube

can

adjusted by

the

eyepiece

tube. Interpupillary

distance adjustment

from

56mm

74mm,

with

photo

tube. Light path selector

lever.

FrS

CrS·VH

Rotatable

graduated mech· Rotatable

graduated

mech·

Stages

anical stage

with

horizontal anical

stage

with

low

drive

drive controls, movement controls, movement range

range

44mm

76mm.

Ver·

52mm

76mm.

Vernier

nier

scales

reading

scales

reading

0.1mm.

Achromatic!

1.40,

with

decenterable aperture iris diaphragm and

aplanatic

graduated

scale.

condenser

Can·

Abbe N.A.

1.25,

with

aperture iris diaphragm and swing-out

densers condenser filter

mount.

CO-4

N.A. 0.85, for super widefield of view,

with

variable

condenser aperture iris diaphragm, filter holder

and

auxiliary

condenser lens.

Photo Eyepieces FK

The

new

photo

eyepieces FKare specially designed

for

photo-

micrography

with

the

Olympus

Photomicrographic

System Camera Model

PM-10

(optional).

The

eyepiece powers available are

2.5x,

3.3x,

and 6.7x. Each

magnification

computed

focus

image at aprojec-

tion

length

125mm

the

same plane

the

35mm

film

plane, thus

compensating spherical aberration.

The

respective magnifications

are engraved on the FKeyepieces:

Total

magnification

picture

when the FK eyepiece

used.

formulated

follows:

Total

magnification

with

35mm

film

=Objective

power

eye-

piece power.

The

formula

below,

however.

will

be applied in

case

the

camp.ra

adapter

with

relay lens, model PM-D L,

used

for

larger

format

camera backs:

Total

magnification

of.large

format

picture

=Objective

power

eyepiece

power

x3.

III

IDENTIFICATION

VARIOUS

COMPONENTS

Trinocular

Observation Tube

Limb

Voltage

Adjustment

Knob

FHT·533

Eyepiece

Revolving

Nosepiece

Objective

5'"",

Conden~r

Voltmeter

DESCRIPTION

EACH COMPONENT

Mic:roMlOpe

Sund

1. Limb 8nd Focusi"9 Mech..,ism

The limb

securely attached

the

sturdy base and supports the observa-

tion tube, stage, condenser, revolving nosepiece and focusing mechanism.

The focusing mechanism includes

the

coarse and fine adjustments and an

automatic pre-focusing lever. This lever

provided

prevent possible

contact between specimen and objective as well as

simplify coarse

focusing.

The

lever

locked after coarse focus has been accomplished.

This prevents further upward travel

the stage and automatically pro-

vides alimiting

stop

the stage

lowered then raised again.

The

automatic pre-focusing lever does

not

restrict fine focusing.

The filter

mount

plaoed

the light exit

the illuminator base.

Stage Clamping Lever

Co_

Adjustment

Knob

Fine

Adjustment

Knob

Automatic

Pre-focusing Lever

FHT

Aux

iory Lens

Shifting Lever

Fillllr

Mount

Light Exit

Coane

Adjustment

Knob

Fine

Adjustment

Knob

Condenser

The condenser may be mounted

the

condenser

mount

by first

inserting

the

condenser into

the

condenser

mount

from below, aligning

the

positioning

dots

the

condenser and condenser mount, and then

clamping with

the

clamping screw. Condenser centration can be

accomplished by means

two

centering knobs. Vertical movement

the

condenser can be adjusted by

the

condenser height adjustment knob.

The condenser has an excellent resolving power,

dry

oil immersion,

from

100x magnification objectives.

When

using

the

100x

objective,

the

distance between condenser and specimen should be

filled with immersion oil.

Note: For use with

the

achromaticlaplanatic condenser N.A. 1.40, the

filter

mount

placed

the

light exit of the illuminator base, while

either

the

Abbe N.A. 1.25

N.A.

0.85

condenser incorporates its

own filter mount. When

the

N.A.

0.85

condenser

used for super

widefield observation, keep

the

auxiliary condenser lens slipped

the

light exit

the

base.

The slide clamping screw permits simultaneous locking of slide and

rotation

condenser and the slide lever allows decentering and rotating

the aperture iris diaphragm for obligue illumination.

•

Abbe

Conden5el",

N.A.

1.25

Abbe

COI"denser

--------

Condenser

Clamping

Screw

Centering

Knob

Filter

Moun't-~--'

•

Achromatic/aplanatic

Condenser,

N.A.

1.4

Achromaticl

aplanatic

Condenser

-------

Condenser

Clamping

Screw

----~-.

Centering

Knob

Aperture

Iris

Diaphragm

Cuntrol

Aing-~-r-'

•CD-4 Condenser,

N.A.

0.85

Condenser

Mount

Aperture

Iris

l..::>

...

_----

Diaphragm

Control

lever

,,~~-

Alignment

Dots

/Condenser

Mount

Slide

Clamping

Screw

......

Alignment

Dots

----Slide

Lever

Condenser

MOllnt

Condenser

Clamping

Screw

Centering

Knob

Filter

Mount

Alignment

Dots

Aperture

Iris

Diaphragm

Control

Leve<

Lever

Position

Fo,

Photomicrography

lOx

Observ.

20x-l00x

Objective

For

Observation

Microscope

Base

and· Light Source

1) Microscope

Base

The lamp house

built

onto

the

base.

The lamp socket

clamped

the lamp house

with

adamping screw. The tight path

selected

with

the auxiliary

lens

shifting lever

for

high, medium and

low

magnification

of objective in use.

...

generally recommended

set the lever

the position marked

with

"Observ." (equivalent

position

for

brightfield observation

with

all the objectives

from

4x through 100x. In phase contrast

darkfield

observation, where

tenser light

required, however,

recommended

set

position M

Haccording

objective magnification

case

with

photomicrography.

Voltage

Adjustment

Knob

Grounding

terminal

..::::::;::;",::::~--

Line

Voltage

Adjustment

Screw

oVoltage Adjustment

The

minimum

voltage required

for

the light source

can

varied

means

the line voltage adjustment screw provided at the back

the

microscope

base

accordance

with

the line voltage and frequency,

since asilicon controlled rectifier (SeRl

.adopted in the dimmer

circuitry.

the

bottom

the

base

avoltage selector switch, which

can

turned

with

acoin,

correspond

with

the voltage

main supply

(11

OV, 120V, 220V or

240Vl.

The transformer

built

the

base

and

switched on and

off

with

the voltage adjustment knob, which also

controls the bulb voltage from 0

lOV.

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