Olympus Fluoview FV1000 Instruction Manual

Olympus FV1000 confocal microscope

Please

•read and follow our guidelines.

•don´t use without introduction by ALMF staff

•Only switch on parts you need for your purposes

(e.g. if you don’t need the Hg lamp for epifluorescence like for overnight on confocal

microscopes)

•report problems to us without delay

Laserlines:

Laser diode 405 nm (DAPI, Photoactivation, Uncaging)

Argon laser: 458, 488, 515 nm (CFP, GFP, YFP, Alexa 488, FITC)

DPSS Laser: 559 nm (RFPs, Cy3, TRITC, Alexa 555/568)

Red Diode Laser: 635 nm (Cy5, Alexa 633/647)

Switching on the system:

•Switch on the PC (button next to floppy drive).

•Switch on the microscope components with the power manager (Mic-

control, PSU1+2, HBO lamp if neccessary)

•Switch on the lasers:

A) Laser combiner (FV10-MCPSU)

B) 559 nm laser if needed

1) Main switch

2) Wait until 'TEMP' is not blinking anymore

3) Turn key to 'on' position

C) Argon-laser if needed:

1) Main switch

2) Key switch

NEW: Always turn off key switch, if the laser is not needed for more

than 1 hour (e.g. next booking >1 hour later) !

•Log on to with the Username "ALMF".

•Start the microscope control software using the icon on the desktop

Switching off

•Check if someone is booked after you on the same day! In case someone is

booked on the same day, just turn the intensity turnknob of the

Argon-laser to the minimum position, switch off the key switch of the

559 nm laser, if there is at least 1 hour to next booking and leave

everything else on.

If nobody else is booked on the same day:

•Turn the Argon laser key (d) to the "Off"-position.

•Exit the control software.

•Switch off the HBO lamp.

•turn the key switch of the 559 nm laser to off and switch off the main

power.

•Switch off the laser combiner.

•Switch off the power manager (black main switch).

•Shutdown the PC.

•Switch the Argon-laser main switch off (c).

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