Millipore Assurance 73400-1BOX User manual

The life science business of Merck KGaA,

Darmstadt, Germany operates as

MilliporeSigma in the U.S. and Canada

Assurance®GDS Adaptor Box

Part No: 73400-1BOX

General Description

The Assurance®GDS Adaptor Box is designed to allow up to 5 Assurance®GDS Rotor-Gene®Q (RGQ) thermocyclers

to be connected to 1 laptop. In addition, the adaptor box improved connectivity between the Assurance® GDS RGQ

and laptop, preventing loss of signal during times of interruption (power loss, networking, etc.). The image below

shows the adaptor box in between an Assurance®GDS RGQ (73070BC) on the left and the Assurance®GDS RGQ

laptop (73071BC) on the right.

Included Components

The Assurance®GDS Adaptor Box contains the following:

•73401 Adaptor Box (iEi uIBX-250-BW-N3/2G-R21)

•82001-1 Ethernet Cable

•82005-1 Power Cable US

•82006-1 Power Supply (110V/220V)

Equipment / Materials Required

Other necessary regional materials not provided, but available separately include:

•82002-1 Power Cable ANZ

•82003-1 Power Cable EU

•82004-1 Power Cable UK

Other necessary materials not provided:

•If networking laptops as described in Section B below, an extra computer monitor screen to plug into the HDMI

port will be needed during setup for viewing the IP address information

•If using WiFi as described in Section B, Step 10, a regional compliant WiFi dongle will be needed during and

after setup

SetUp Procedures

There are two options:

A. No Network, 1 Assurance®GDS RGQ to 1 Laptop Interface

B. Network, Multiple Assurance®GDS RGQ to 1 Laptop Interface

Please follow the procedure per the chosen option as shown below.

Page 2 of 14

A. No Network, 1 Assurance®GDS RGQ to 1 Laptop Interface

Follow the procedure below to setup the Assurance®GDS RGQ with the Assurance®GDS Adaptor Box

when using without online networking. If networking the laptop, please go directly to Section B, below.

Note: without a network setup, the platform can only accommodate a 1:1 ratio between Assurance®GDS

RGQ-Adapter and laptop.

1. Connect the Assurance®GDS adaptor box to the Assurance®GDS RGQ with the standard USB cable

and to the controlling laptop with a standard CAT5/6 network cable. The network cable must be

plugged into port 1. [See images below]

2. Turn on laptop and navigate to the “Network Connections” panel [see images below]

Windows 10

Page 3 of 14

Windows 7

3. Select the Ethernet adaptor box; right-click on the icon and select “Properties” [see images below]

Note: Multiple icons may be present. Be sure to select the “Ethernet” icon. Windows 7 may say “Local

Area Connection” instead of “Ethernet”

Windows 10

Example of numerous icons: Select “Ethernet”

Windows 7

4. In the Ethernet Properties dialogue box, select “Internet Protocol Version 4” and then select

“properties” [see image below]

Note: there may be some additional end characters like “(TCP/Pv4)” or something similar

Page 4 of 14

5. In the “Internet Protocol Version 4 Properties” dialogue box fill in the following information for each

section [see image below], IP address: “192.168.1.10”, Subnet mask: “255.255.255.0” [SHOULD

AUOTFILL], Select “OK”

6. Start the Assurance®GDS RGQ laptop software

7. Navigate to the configuration dialog menu [see images]

Select “Setup”

Page 5 of 14

8. In the configuration dialog, enter [see image below for example]

RGQ serial number

RGQ NickName; optional

RGQ Location; optional

Base URL: fill in “http://192.168.1.20:8080/”

Interface: select “ethernet”

IP Config: select “static”

Netmask: fill in “255.255.255.0”

Leave remaining fields in default state

Save when completed

9. After the adaptor box configuration file is set up, save the configuration on a blank, standard USB

drive. [saving a backup of the “rgq.cfg” file on a computer or network is recommended]

Page 6 of 14

Note: The software will select an empty USB drive by default if one is connected to the laptop [see image

below]

10. Turn on the Assurance®GDS RGQ and Assurance®GDS adaptor box

Note: the Assurance®GDS adaptor box power buttons are designed to prevent accidental turn on/off

actions. To power on/off the adaptor box, hold the power button until button lights up [power on] or

hold power button until button light turns off [power off].

11. Insert the USB drive into each Assurance®GDS adaptor box one at a time. Ensure the respective

Assurance®GDS RGQs are connected to the Assurance®GDS adaptor boxes via USB and are turned

on.

•On recognizing the drive, the Assurance®GDS adaptor box will copy over the correct configuration

determined by the serial number of the connected Assurance®GDS RGQ.

oSeveral beep sequences will follow once the USB drive is inserted. Wait for all sequences

to finish [there will be a final two-tone chime to designate the end of all sequences]

oIf the configuration process is successful, the Assurance®GDS adaptor box will chime with

a rising three tone sequence followed by the two-tone ending sequence and the box will

become available in the laptop software [success sequence will be slow]

o[if this beep does not occur, reformat the stick to FAT or try a different stick]

oIf there is a problem with the configuration, the system will chime a three-tone decanting

sequence [failure sequence will be fast]

•The configuration sequence will generate a set of addition files on the USB drive. These are

diagnostic files that can be used for troubleshooting should any errors occur. Saving the additional

files is recommend until the user is confident the system is functioning properly. Each Assurance®

GDS adaptor box will write its configuration and errors into a file named RCB_[0123456789ab].txt

on the USB drive, where the number in the [brackets] is the primary ethernet adaptor box MAC

address. The MAC address is unique to each box.

12. Restart Assurance®GDS adaptor box and wait for connection to be made between software and box:

•User should see tab with Assurance®GDS RGQ serial number and Nickname upon restarting the

Assurance®GDS software after configuration saving.

Page 7 of 14

•When first configuring a new setup, the icon should change from the yellow “connection lost” to

the blue “empty instrument” icon when connection is established.

•Setup now complete. Corresponding instrument icons are as follows and can be used for

diagnosing connection status:

= Setup Mode Tab | Used for setting up new runs to be sent to

open instruments. Can also be used to read run files. Note: the virtual mode tab is always present.

= Empty Instrument | Instrument cleared and ready to receive

new run file. Note: instruments must be cleared before a new run can be sent to the instrument.

= Run Complete | Run has finished, and results are ready to be

read, saved, exported, etc.

= Connection Lost | Connection to the Assurance®GDS adaptor

box or Assurance®GDS RGQ has been broken and must be established before further action.

= Run in Progress | Run has been initiated and instrument is

taking readings.

= Instrument Error | problem with instrument. Seek technical

assistance.

B. Network, Multiple Assurance®GDS RGQ to 1 Laptop Interface

Follow the procedure below to setup the Assurance®GDS RGQ with the Assurance®GDS Adaptor Box

when networking your laptop [perform with the assistance of your local IT department]:

1. Connect all adaptor boxes to the user’s network. The network cable must be plugged into port 1. [See

image of hardline connection below]

•For wireless connection see Step 10 at end of Section B.

Note: User MUST select wired OR wireless connection per adapter; both wireless and wired

connections are NOT possible on a single adapter unit.

•Turn Assurance®GDS adaptor boxes on after connecting to network.

Note: the Assurance®GDS adaptor box power buttons are designed to prevent accidental turn

on/off actions. To power on/off the Assurance®GDS adaptor box, hold the power button until

button lights up [power on] or hold power button until button light turns off [power off].

Page 8 of 14

2. Turn on each adaptor box and acquire the IP addresses for each box by plugging a standard computer

monitor into each box [recommend using HDMI cable; see images below].

•If preassigning IP see Step 11 at end of Section B.

•For IT department: If you use the box headless, use the logs of your DHCP server to locate the

box IP address.

oNote: If you use DHCP, fix the address of the adaptor box on the DHCP-Server. Otherwise,

the box may change its IP over time and thus become inaccessible to the laptop software.

If not using DHCP, the IP addresses of the box will be set in Step 11. The adaptor box will

take a few moments to acquire the IP address. The Screen will show a generic IP address

upon start following a 192.168.66.1 format [see image below]. Once the network IP

address is acquired from DHCP, the screen will show the acquired IP address [see image

below]

Page 9 of 14

3. Connect all adaptors to their respective Assurance®GDS RGQ [see image below]

4. Start the Assurance®GDS RGQ laptop software

5. Navigate to the configuration dialog menu [see images]

Page 10 of 14

Select “Setup”

6. In the configuration dialog, enter…

•the IP addresses for all adaptor boxes

•the corresponding serial numbers of each Assurance®GDS RGQ [be careful to match the correct

Assurance®GDS RGQ to the correct Assurance®GDS adaptor box]

•use port 8080 (default)

•enter the IP address assigned by the network (DHCP). [if a predesignated IP address is used,

enter the static IP]

Note: the URL format of in the example below. The IP address assigned by your DHCP should always

be followed by “:8080/” or the software will not accept the input. “RGQ NickName” and “RGQ

Location” are optional.

Fill in a new Assurance®GDS adaptor box settings dialogue box for each Assurance®GDS adaptor box-

RGQ pair using the “Add” button consecutively.

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