Thermo Scientific Shake n Stack 6240 Operating instructions

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User Manual

Shake ‘n Stack

Hybridisation Oven

Operating and Maintenance Manual 7026240 Rev. 1

Thermo Scientific

Manual Number 7026240

1 ECNM-000224 02/06/2020 Removed model numbers 6244 & 6245 from the manual. TFI

0 25036/OV-368 8/16/11 New controller - Release 3 ccs

EV EC /ECN DATE DESC IPTION By

Preface

Shake ‘n Stack i

Models covered by this manual

Models Voltage Includes

6240 220 Shaking platform, drip tray, rotisserie

6241 110 Shaking platform, drip tray, rotisserie

6242 220 Drip tray, rotisserie

6243 110 Drip tray, rotisserie

Thermo Scientificii Shake ‘n Stack

Preface

Contains Parts and Assemblies

Susceptible to Damage by

Electrostatic Discharge (ESD)

CAUTION

Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual

may result in damage to the unit, injury to operating personnel, and poor equipment performance. s

Caution All internal adjustments and maintenance must be performed by qualified service personnel. s

Material in this manual is for information purposes only. The contents and the product it describes are subject

to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this

manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to

the use of this manual.

Thermo Scientific Shake ‘n Stack iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this

symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.

ot surface(s) present which may cause burns to unprotected skin, or to materials which may be

damaged by elevated temperatures.

Marking of electrical and electronic equipment, which applies to electrical and electronic equipment

falling under the Directive 2012/19/EU (WEEE) and the equipment that has been put on the market

after 13 August 2005.

This product is required to comply with the European Union’s Waste Electrical & Electronic

Equipment (WEEE) Directive 2012/19/EU. It is marked with the WEEE symbol. Thermo Fisher

Scientific has contracted with one or more recycling/disposal companies in each EU Member State

European Country, and this product should be disposed of or recycled through them. Further

information on Thermo’s compliance with this directive, the recyclers in your country and

information on Thermo Scientific products will be available at www.thermofisher.com.

4Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4Always dissipate extreme cold or heat and wear protective clothing.

4Always follow good hygiene practices.

4Each individual is responsible for his or her own safety.

Thermo Scientificiv Shake ‘n Stack

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct

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service.led.marietta@thermofisher.com Tech Support Email Address

Certified Service Web Pagewww.unitylabservices.com

Our staff can provide information on pricing and give you quotations. We canSales Support

take your order and provide delivery information on major equipment items or make

arrangements to have your local sales representative contact you. Our products are listed on the

Internet and we can be contacted through our Internet home page.

Our staff can supply technical information about proper setup, operation orService Support

troubleshooting of your equipment. We can fill your needs for spare or replacement parts or

provide you with on-site service. We can also provide you with a quotation on our Extended

Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your

applications. If you are experiencing technical problems, working together, we will help you

locate the problem and, chances are, correct it yourself...over the telephone without a service

call.

When more extensive service is necessary, we will assist you with direct factory trained

technicians or a qualified service organization for on-the-spot repair. If your service need is

covered by the warranty, we will arrange for the unit to be repaired at our expense and to your

satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you

Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by

telephone or fax. If you wish to write, our mailing address is:

Thermo Fisher Scientific (Asheville) LLC

401 Millcreek Road, Box 649

Marietta, OH 45750

International customers, please contact your local Thermo Scientific distributor.

Thermo Scientific

Table of Contents

Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Oven Temperature Monitoring . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

Unpacking and Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

Set the Required Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-2

Set Up the Shaking Platform . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Methodology of Hybridisation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Place Membranes in a Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Insert Membranes in a Bottle . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2

Pre- ybridisation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3

ybridisation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3

Washing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4

Washing in ybridisation Bottles . . . . . . . . . . . . . . . . . . . . . . . . . .4-4

Washing in a Sandwich Box . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-4

otisseries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

Variable Axis Rotisseries . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1

50ml & 15ml Tube Rotisseries . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Section 1

Section 2

Section 3

Section 4

Section 5

Shake ‘n Stack v

vi Shake ‘n Stack Thermo Scientific

Cleaning and Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Meshes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Bottles & Caps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Oven Interior/Shaking Platform . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Rotisserie . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Use of adioactive Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

Additional Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3

Exploded Parts Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-4

Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

Warranty Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1

Troubleshooting Guide for Nucleic Acid Hybridisations . . . . . . . . .AI-1

Background Reduction - General . . . . . . . . . . . . . . . . . . . . . . . . . . .AI-1

Factors Resulting in igh Backgrounds . . . . . . . . . . . . . . . . . . . . . .AI-3

Factors Resulting in Signal Lower than Expected . . . . . . . . . . . . . . .AI-4

Hybidisation Bottle Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .AII-1

Bottle Care . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .AII-1

Table of Contents

Section 6

Section 8

Section 7

Section 9

Section 10

Appendix I

Appendix II

Shake ‘n Stack 1-1Thermo Scientific

Oven Temperature

Monitoring

Section 1 Introduction

The Thermo system of ybridising in bottles is considered by many

molecular biologists to be the best method for conducting ybridisations

with Southern, Northern, Dot, Slot or Colony Blots. Additional

information, including detailed protocols, is contained in the included

ybridisation Guide.

ybridising in bottles means that probe volumes may be significantly

reduced compared to experiments performed in conventional systems, and

the continual movement of the probe across the surface of the membrane

results in very efficient hybridisation reactions.

Protection from exposure to radiation is provided by the heavy walled

borosilicate glass bottles.

Warning Based on tests, these bottles block almost 100% of BETA

radiation emissions of a 1,000 pCi P-32 source. owever, users should

observe precautions due to induced X-ray phenomenon (Breemsstrahlung)

occurring, depending on the type(s) of radionuclides used. s

The ybridisation Oven itself provides additional shielding. In the event

of a spillage within the Oven, the stainless steel drip tray will contain up to

6.8 fl. oz. (200 ml) of liquid.

Note If the Shake ‘n Stack is not used as specified in this manual, the

protection provided by the equipment may be impaired.

All Ovens are calibrated by matching the thermistor temperature read-out,

seen on the LCD display, to the actual measurement of distilled water

contained within a ybridisation Bottle as it rotates. The temperature

measuring device used inside the calibration bottle is traceable to national

standards.

This calibration method ensures all experiments using the rotisserie meet

our temperature accuracy specification of ±1°C and indeed, due to the

rotisserie action, uniformity within the bottle is ±0.25°C. owever, it is

important to note that the temperature inside the oven chamber will vary

from point to point and therefore, the temperature of objects/vessels,

which are not placed in the rotisserie, will vary from that which is

displayed. Refer to “Setting the Required Temperature” for

recommendations on temperature setting when using shaking platforms.

1-2 Shake ‘n Stack Thermo Scientific

Section 1

Introduction

The Shake ‘n Stack is designed to be used with Thermo’s hybridisation

bottle. These high quality leak-proof containers can be used at

temperatures up to 70°C (for catalogue numbers, see Section 8).

Bottles should not be used above 70°C without taking the precaution of

releasing the pressure formed by the release of gases from solutions as they

are warmed. Simply loosening, then retightening the bottle cap at

approximately 60°C should relieve the pressure.

Caution Dropping or over-tightening lids can cause cracking and failure.

Lids should be inspected prior to each use. s

Caution Always wear gloves when handling the bottle and use the bottle

gripper provided (see Appendix II for full instructions on Bottle Care). s

Finally, the Shake ‘n Stack Oven is designed for reliability and ease of

maintenance. The rotisserie, shaking platform and drip tray can be easily

removed for cleaning.

Supply

Power rating: . . . . . . . . . . . . . . . . . . . . . . . . .250 W

Frequency: . . . . . . . . . . . . . . . . . . . . . . . . .50/60 z

Fuse rating: . . . . . . . . . . . . .110/120 V: 6.3A type T

. . . . . . . . . . . . . . . . . . .220/240 V: 1.6A type T

Ingress Protection Rating . . . . . . . . . . . . . . . . .IP22

Rotisserie speed control PCB fuse: . . . . . . . .500 mA

Note This item should not be serviced by the customer.

Working Environment: . . . . . . . . . . . .15°C to 25°C

Caution Care should be taken when lifting the Oven. It is advisable that

help is sought when removing an Oven from its packaging and when

stacking Ovens for use. s

Caution During operation, caution should be taken with moving parts

that are accessible when the Oven door is open. s

Thermo offers full service and technical support for all its products.

Alternatively, contact your local authorised distributor.

Safety Precautions

Oven Temperature

Monitoring (cont’d)

Shake ‘n Stack 2-1Thermo Scientific

Section 2 Unpacking and Installation

Shake ‘n Stack Oven

4 Leveling Feet

Shaking Tray (Models 6240, 6241)

Shaking Tray Drive Arm Assembly (Models 6240, 6241)

Shaking Tray Bracket (Models 6240, 6241)

Power Cable

Drip Tray

Note If any item is missing or damaged, contact Thermo or an authorised

Thermo distributor.

Warning If it is intended to use the Oven with radioactive isotopes, the

oven must be located in a designated Radiation Area. Radiation safety

procedures must be followed at all times. s

• In the event of spillage, refer to Section 6 for guidance on cleaning and

decontamination.

• All ybridisation Oven standard rotisseries are designed to accept the

long, medium or short ybridisation Bottles.

Once unpacked, attach leveling feet to base, and position on a flat surface.

Level the oven by adjusting the height of the leveling feet. Once leveled,

the oven is ready for use. If adding to a stack of Shake ‘n Stack Ovens

(maximum of 3), attach feet and adjust height, then position feet in the 4

indents on the top of the supporting Shake ‘n Stack Oven. Some

additional adjustment of the feet may be necessary until the ovens sit in a

stable position.

Note Shake ‘n Stack Ovens have been designed to stack 3 units high. No

attempt should be made to stack higher than this.

Connect each individual oven to the mains supply using only the

removable mains lead supplied. The mains lead should be fitted with a

10 A fuse for both 110/120 V and 220/240 V regions. The oven is

grounded via the mains lead and should only be connected to an grounded

supply. (See “Technical Specifications”).

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