Thermo Scientific 3403 User manual

Midi 40 CO2 Incubator

Models 3403 and 3404

Service Manual 7993403 Rev. 2

_________________________________________________________________________________

Part of Thermo Scientific

Thermo Scientific

Manual Number 7993403

2 28728 6/18/12 Added full CO2 calibration procedure - Section 7 ccs

1 27807 10/3/11 Updated with op manual revisions ccs

0 -- 12/10/09 Original, was 97665H08 ccs

REV ECR/ECN DATE DESCRIPTION By

Preface

Midi 40 CO2 Incubator i

Thermo Scientificii Midi 40 CO2 Incubator

Preface

Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual

may result in damage to the unit, injury to operating personnel, and poor equipment performance. s

Caution All internal adjustments and maintenance must be performed by qualified service personnel. s

Material in this manual is for information purposes only. The contents and the product it describes are subject to

change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this

manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to

the use of this manual.

Progressive product changes will be entered into the service manual as it is revised. The manual is intended as an

aid to persons qualified in the service of applicable equipment. It is not intended to teach unqualified persons

on applicable equipment all procedures necessary to make repairs.

Thermo Scientific Midi 40 CO2 Incubator iii

Preface

Important operating and/or maintenance instructions. Read the accompanying text carefully.

Potential electrical hazards. Only qualified persons should perform procedures associated with this

symbol.

Equipment being maintained or serviced must be turned off and locked off to prevent possible injury.

Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be

damaged by elevated temperatures.

Marking of electrical and electronic equipment, which applies to electrical and electronic equipment

falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market

after 13 August 2005.

This product is required to comply with the European Union’s Waste Electrical & Electronic

Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher

Scientific has contracted with one or more recycling/disposal companies in each EU Member State

European Country, and this product should be disposed of or recycled through them. Further

information on Thermo’s compliance with this directive, the recyclers in your country and

information on Thermo products will be available at www.thermo.com.

4Always use the proper protective equipment (clothing, gloves, goggles, etc.)

4Always dissipate extreme cold or heat and wear protective clothing.

4Always follow good hygiene practices.

4Each individual is responsible for his or her own safety.

Thermo Scientificiv Midi 40 CO2 Incubator

Preface

Do You Need Information or Assistance on

Thermo Scientific Products?

If you do, please contact us 8:00 a.m. to 6:00 p.m. (Eastern Time) at:

1-740-373-4763 Direct

1-800-438-4851 Toll Free, U.S. and Canada

1-740-373-4189 FAX

http://www.thermo.com Internet Worldwide Web Home Page

Service E-Mail Address

Thermo Scientific

Controlled Environment Equipment

401 Millcreek Road, Box 649

Marietta, OH 45750

Our staff can provide information on pricing and give you quotations. We can

take your order and provide delivery information on major equipment items or make

arrangements to have your local sales representative contact you. Our products are listed on the

Internet and we can be contacted through our Internet home page.

Our staff can supply technical information about proper setup, operation or

troubleshooting of your equipment. We can fill your needs for spare or replacement parts or

provide you with on-site service. We can also provide you with a quotation on our Extended

Warranty for your Thermo Scientific products.

Whatever Thermo Scientific products you need or use, we will be happy to discuss your

applications. If you are experiencing technical problems, working together, we will help you

locate the problem and, chances are, correct it yourself...over the telephone without a service

call.

When more extensive service is necessary, we will assist you with direct factory trained

technicians or a qualified service organization for on-the-spot repair. If your service need is

covered by the warranty, we will arrange for the unit to be repaired at our expense and to your

satisfaction.

Regardless of your needs, our professional telephone technicians are available to assist you

Monday through Friday from 8:00 a.m. to 6:00 p.m. Eastern Time. Please contact us by

telephone or fax. If you wish to write, our mailing address is:

International customers, please contact your local Thermo Scientific distributor.

Sales Support

Service Support

service.led.marietta@thermofisher.com

Midi 40 CO2 Incubator vThermo Scientific

Table of Contents

General Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1

Included Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-2

Wiring Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

CO2 Gas Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

Model and Serial Number . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-3

Component Locations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-4

Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1

Technical Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Cabinet Construction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Microprocessor Control Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Features and Benefits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1

Alarm Monitoring System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2

Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2

Control Systems Theory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Door Heat System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Overtemperature Monitoring System . . . . . . . . . . . . . . . . . . . . . . . . .4-1

CO2 Control Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Temperature Control System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1

Preventive Maintenance and Diagnostics . . . . . . . . . . . . . . . . . . . . . . .5-1

Recommended Tool List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-2

Troubleshooting Parameter List . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3

Troubleshooting Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-4

Section 1

Section 2

Section 3

Section 4

Section 5

vi Midi 40 CO2 Incubator Thermo Scientific

Maintenance & Repair Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Replacing the Gas Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1

Replacing the Temperature Sensor . . . . . . . . . . . . . . . . . . . . . . . . . . .6-2

Replacing CO2 Sensor and Interface PCB . . . . . . . . . . . . . . . . . . . . .6-3

Replacing Printed Circuit Boards . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-4

Testing the Outer Door Heater . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-5

Replacing the Outer Door Heater . . . . . . . . . . . . . . . . . . . . . . . . . . .6-6

Replacing CO2 Solenoid Assembly . . . . . . . . . . . . . . . . . . . . . . . . . .6-7

Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Access Program or Service Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1

Temperature Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-2

CO2 Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-3

Appendices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

IntrLogic Power/Key On Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1

IntrLogic Test Points and Jumpers . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2

Common Diagnostics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-2

How to Enter Test Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3

IntrLogic: Board Test Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-3

Telemetry Tables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-5

Detailed Descriptions of IntrLogic PCBs . . . . . . . . . . . . . . . . . . . . .8-10

Relay and Triac Allocation on Power PCB . . . . . . . . . . . . . . . . . .8-11

General Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-13

Interface PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-20

CO2 Sensor PCB . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-24

Electrical Schematic and Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1

Parts List . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-2

Table of Contents

Section 6

Section 8

Section 7

Section 9

Midi 40 CO2 Incubator 1-1Thermo Scientific

Section 1 General Information

The purpose of this manual is to provide procedures developed to assist

the field refrigeration technician in troubleshooting and repairing certain

conditions.

The incubators described in this manual are classified for use as stationary

equipment in a Pollution Degree 2 and Overvoltage Category II

environment, according to the UL3101-1 and IEC 664 standards.

These units are designed to operate under the following environmental

conditions:

• Indoor use

• Altitude up to 2000m

• Maximum relative humidity 80% for temperatures up to 31°C

• Main supply voltage fluctuations not to exceed 10% of the nominal

voltage.

Symphony incubators are designed to create a stable, reliable environment

for cell culture applications. They operate at temperatures ranging from

5°C above ambient temperature to +40°C, accurate to ±0.2°C. The gas

system controls within ±0.1% of measurable setpoint.

Each chamber consists of two sections: the cabinet (where the product is

stored) and the control section (containing the temperature, CO2, and

humidity control systems). On single-chamber models, the control section

is to the side of the cabinet.

Table 1: Typical Dimensions

Height Width Front to Back

18 in. 23.5 in. 18.5 in.

46.7 cm 59.7 cm 47 cm

1-2 Midi 40 CO2 Incubator Thermo Scientific

Section 1

General Information

Locate the unit in a level area free from vibration with a minimum of

three inches (7.6 cm) of space on the sides and rear and 12 inches (30.5

cm) at the top. The table must be able to support 40 PSI. Also, allow

enough clearance so that the door can swing open at least 90 degrees, and

the power cord can easily be reached to disconnect power. Do not

position the equipment in direct sunlight or near any HVAC

duct/diffusers. The ambient temperature range at the location must be

59°F to 90°F (15° to 32°C).

The following items are packaged and shipped inside the incubator

cabinet:

• The operating manual

• Shelves and shelf brackets

• 1/4 in. (476 mm) ID clear tubing for the gas connection

• Cordset

• 5” round humidity pan

• Additional CO2sensor gasket

•CO

2disposable filter 99.97

Location

Included Parts

Figure 4-1. Included Components (shelves and brackets not shown)

Cordset

Additional

gasket

Humidity

pan

Tubing

Filter

Operating

manual

Midi 40 CO2 Incubator 1-3Thermo Scientific

Section 1

General Information

Verify that the incubator gas supply is available near the installation area.

The required gas supply pressure is 15 PSI, controlled by a high-quality,

two-stage regulator suitable for the connected input gas.

Do not connect the gas at this time.

Caution Connect the equipment to the correct power source. Be sure to

operate the incubator at the voltage specified on the dataplate. Incorrect

voltage can result in severe damage to the equipment. s

Warning Failure to properly ground the equipment may cause personal

injury or damage to the equipment. s

Caution Always conform to National Electrical Code and local codes. s

Warning Do not connect the unit to overloaded power lines. s

The incubators described in this manual are rated for supply voltage

115/120VAC, single-phase, 60Hz, 3 amps.

Always connect the unit to a dedicated (separate) circuit. Do not exceed

the electrical and temperature ratings printed on the data plate. Electrical

codes require fuse or circuit breaker protection for branch circuit

conductors.

Model numbers are important because they identify the unit for service

capability. The serial number is a unique number that identifies the unit

as a specific unit. No other unit has that same number.

The model and serial numbers of your incubator are printed on a plastic

identification plate, commonly referred to as the data plate. The most

important information concerning your unit can be gathered from the

model number and the serial number identification. Additional

information is available on the data plate; such as the voltage, frequency,

and current ratings.

The data plate is mounted on the outside of the cabinet, beside the upper

door hinge. Some older models may have the plate mounted on the front

of the frame, just above the lower door hinge.

Model and Serial

Number

CO2 Gas Supply

Wiring Cautions

This manual suits for next models

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