PerkinElmer Lambda 365 User manual

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PerkinElmer, 710 Bridgeport Avenue,

Shelton, CT06484-4794, U.S.A

Produced in the USA.

Lambda 365 Water Jacketed 8-Position Multi-Cell Holder

Installation Instructions

This instruction sheet describes the installation of this accessory which is used with the Lambda 365

Spectrophotometer.

NOTE:

Read these instructions before you install this accessory.

Contacting PerkinElmer

Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part

numbers.

See our website:

http://perkinelmer.com

PerkinElmer's catalog service offers a full selection of high-quality supplies.

To place an order for supplies and many replacement parts, request a free catalog, or ask for information:

If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be

shipped promptly, usually within 24 hours.

If you are located outside of the U.S., call your local PerkinElmer sales or service office.

Features

•Suitable size for the standard cells

•Excellent durability

•Controls the temperature by circulation of liquid

Figure 1 Lambda 365 Water Jacketed 8-Position Multi-Cell Holder [P/N: N4101006]

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Dimensions and Specifications

Dimensions

Physical Characteristic Specification Comment

Outline

Height (mm) 143

Width (mm) 135

Depth (mm) 354

Inner

Height (mm) 35

Suitable for the Standard

Cell

Width (mm) 14

Depth (mm) 12.6

Weight (kg) 2.3

Specifications

Physical Characteristic Specification Comment

Space between cells (mm) 10.4

Moving distance of one cell (mm) 11.5

Moving distance of eight cells (mm) 80.5

Moving time of one cell (sec) 1.3

Moving time of eight cells (sec) 8.5

Environmental Conditions for Operation

Specification Comment

RT~ 100℃Controls the temperature using Circulator

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Configuration of Water Jacketed 8-Position Multi-Cell Holder

Figure 2 Water Jacketed 8-Position Multi-Cell Holder Configuration

- Used to fix a cell holder, a base plate or a front plate for Lambda 365.

- Spare screws (2ea) are enveloped with the accessory.

Figure 3 Phillips round headscrew with washer (M4 *12L)

Figure 4. Male connector of quick coupling (2 each)

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Installation

1. Prepare the Lambda 365 Spectrophotometer to install this accessory.

2. Connect the power cord and the communication cable. DO NOT turn on the power of the

instrument!

3. Remove the two Phillips round head screws with washer (M4*12L) to disassemble the existing cell

holder and base plate

Figure 5. Location of the Phillips round head screws with washer

4. Pull out the cell holder and base plate by hand.

Figure 6. Pulling out the cell holder and base plate

CAUTION

ATTENTION

Make sure the instrument is turned off while installing this accessory.

Assurez-vous que l’instrument est é

teint lors de l’installation de cet

accessoire.

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5. Insert the Water Jacketed 8-Position Multi-Cell Holder in the sample compartment.

Figure 7. Inserting the Water Jacketed 8-Position Multi-Cell Holder into the sample

compartment

6. Gently press the Water Jacketed 8-Position Multi-Cell Holder to connect the communication port

(male) under the bottom of the Water Jacketed 8-Position Multi-Cell Holder to the port (female) in

the sample compartment.

Figure 8. Connecting the communication ports

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7. Tighten the Water Jacketed 8-Position Multi-Cell Holder in the sample compartment with the

screws. (The red circles indicates the location of the screws in the figure).

Figure 9. Tighten the Water Jacketed 8-Position Multi-Cell Holder

8. Assemble the tube with the male connector of quick coupling.

Figure 10. Assembling the tube with the male connector

NOTE:

The tube size is recommended 6 mm-ID and 10 mm-OD silicon tube which has thermal resistance at least

100ºC.

9. Connect the male connector with the tube to the quick coupling mount of the front plate.

Figure 11. Connecting the male connector to the quick coupling

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10. Turn on the power switch of the instruments.

Measurement

NOTE:

Before executing System Self Test,the Water Jacketed 8-Position Multi-Cell Holder has to be installed first.

Otherwise, the instrument can be damaged electrically and do not operate properly.

1. Install the Water Jacketed 8-Position Multi-Cell Holder.

2. Close the sample compartment cover and turn on the instrument.

CAUTION

not

start the UV Express software before finishing the initialization of grating.

NOTE:

Start the System Self Test after warming up the system for at least 20 minutes.

3. Double-click on the UV Express Software folder and select one of the mode for starting.

4. The following message box will be shown. Empty the cell holder and close the lid firmly. Click OK.

5. System Self Test stars. Click OK after finishing the System Self Test.

6. Select New to open a new window. Select Experiment Type and select OK.

7. Open Method in the main menu.

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8. ①Select the Accessory tab and ②Select Multi-cell on Accessory Type, and then click the

③Method Setup button to open the Multicell window.

9. Using the button, select cells to be used among the available cells list. Check (√) Multi

Baseline or Multi Zero, if it is required to use.

a. Available Cells: Indicate cells that are available for measurement.

b. Using Cells: Shows the cell positions which are selected for measurement. Remove cells

using key and switch positions using and keys.

c. Multicell Options

Multi Zero: If checked (√), zero will be measured all the selected positions. If not, zero will

be only measured at the 1st cell position among the using cells.

Multi Baseline: If checked (√), the baseline will be measured at each selected cell. If not

checked, the baseline is only measured at the 1st cell position among the using cells.

NOTE:

To prevent inadequate baseline (zero) measurement because of the difference in transmittance among

cuvettes or any other reasons, Multi Baseline (Multi Zero) measurement is recommended.

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10. Click OK.

11. After setting parameters for Experiment, Lamp, Internal Reference and Scan Setup, click

Apply and then select OK.

12. After placing the reference solution into the selected Using Cell positions and the reference cell

position of the Multicell holder, click the Baseline or Zero button to perform the Baseline or Zero

function.

NOTE:

Baseline is required to run only one time when the first measurement is done, not every measurement.

However, it should be done whenever the system is powered off/on, the wavelength is changed, SBW is

changed or Reference sample is changed.

13. Place the reference into the reference cell position and sample into the selected Using cell

positions

14. Select the Sample button to measure.

15. After the measurement is finished, the results are displayed in the result window. Save or print

the results as required.

Calibration of Multi-Cell Position

Calibrate the beam position of the Water Jacketed 8-Position Multi-Cell Holder when the Multicell is

installed for the first time or beam position is incorrect.

1. Execute Scan Setup mode and ①click the Accessory tab of Method window. ②Select Multi-

cell on Accessory Type, and then click the ③Method Setup button to open the Multicell

window.

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2. Select the Calibration tab in the Multicell window. The following dialog box will appear.

3. The functions of the Multicell Calibration are shown as follows.

Command Function

MultiCell Position Show saved steps about each cell position of the Multi-Cell.

Multicell Move

Used for moving Multi-Cell position as clicking buttons

Used for moving Multi-Cell position using

buttons by

entered step.

Multicell Calibration Used to perform the Multi-Cell calibration.

Stop Used to stop the Multi-Cell calibration.

Save Result Used to save the calibrated result.

Reset Multicell Used to move to ‘0’ step of Multi-Cell position.

4. Click on Reset Multicell to format the Multi-Cell steps. Click OK.

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